|Institutional Source||Beutler Lab|
|Gene Name||interleukin 16|
|Is this an essential gene?||Probably non essential (E-score: 0.234)|
|Stock #||R1018 (G1)|
|Chromosomal Location||83642825-83745726 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 83674538 bp|
|Amino Acid Change||Asparagine to Serine at position 268 (N268S)|
|Ref Sequence||ENSEMBL: ENSMUSP00000001792 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000001792] [ENSMUST00000153560]|
|Predicted Effect||probably damaging
AA Change: N268S
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: N268S
|Predicted Effect||probably benign
|Meta Mutation Damage Score||0.2461|
|Coding Region Coverage||
|Validation Efficiency||100% (50/50)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
PHENOTYPE: Mice homozygous for a knock-out allele display a transient but consistent increase of thymidine incorporation in anti-CD3-stimulated CD4+ T cells, but fail to show a hyperproliferative T cell phenotype using BrdU labeling. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Il16||
(F):5'- ACCTTGCGGAAGAAAGTGATGCTG -3'
(R):5'- TCAAGCATGGTGAATCCAAAGGCTAA -3'
(F):5'- agagcagaacagggagagag -3'
(R):5'- CTAAGAGCAAGGTTTGGAGAGC -3'