Incidental Mutation 'R0980:Trim66'
ID 97001
Institutional Source Beutler Lab
Gene Symbol Trim66
Ensembl Gene ENSMUSG00000031026
Gene Name tripartite motif-containing 66
Synonyms Tif1d, D7H11orf29
MMRRC Submission 039106-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.169) question?
Stock # R0980 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 109048213-109107341 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to A at 109054877 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Leucine at position 1240 (V1240L)
Ref Sequence ENSEMBL: ENSMUSP00000102352 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]
AlphaFold Q924W6
Predicted Effect probably damaging
Transcript: ENSMUST00000033339
AA Change: V1138L

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026
AA Change: V1138L

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000106739
AA Change: V1138L

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026
AA Change: V1138L

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000106741
AA Change: V1240L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026
AA Change: V1240L

DomainStartEndE-ValueType
RING 28 78 2.38e-2 SMART
BBOX 102 140 1.48e0 SMART
PHD 106 171 7.77e0 SMART
RING 107 170 4.38e0 SMART
BBOX 162 203 4.21e-3 SMART
BBC 210 336 1.61e-39 SMART
low complexity region 420 435 N/A INTRINSIC
low complexity region 554 588 N/A INTRINSIC
low complexity region 619 632 N/A INTRINSIC
low complexity region 670 683 N/A INTRINSIC
PHD 1100 1143 4.09e-10 SMART
BROMO 1171 1277 8.22e-27 SMART
low complexity region 1287 1301 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.0%
  • 10x: 95.1%
  • 20x: 89.2%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 46 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ankdd1a T C 9: 65,424,253 (GRCm39) H20R probably damaging Het
Arfgef3 T C 10: 18,467,866 (GRCm39) E1778G possibly damaging Het
Arhgef18 C T 8: 3,439,095 (GRCm39) probably benign Het
Blm A T 7: 80,149,706 (GRCm39) probably null Het
Ccr6 A G 17: 8,474,846 (GRCm39) E17G probably benign Het
Cep126 T C 9: 8,100,720 (GRCm39) T605A probably damaging Het
Cnga4 C A 7: 105,057,213 (GRCm39) P439T probably damaging Het
Col11a1 G A 3: 113,932,414 (GRCm39) R113H unknown Het
Cyp2a5 A G 7: 26,538,431 (GRCm39) probably null Het
D430041D05Rik A G 2: 104,079,690 (GRCm39) V1131A probably damaging Het
Elp3 T C 14: 65,815,402 (GRCm39) T197A probably damaging Het
Etl4 A G 2: 20,806,378 (GRCm39) D1200G probably damaging Het
Gapt G C 13: 110,490,273 (GRCm39) T130R probably damaging Het
Gprin1 T C 13: 54,888,214 (GRCm39) D20G possibly damaging Het
Hltf T C 3: 20,145,665 (GRCm39) S432P probably benign Het
Immt T C 6: 71,851,310 (GRCm39) V54A probably benign Het
Jhy T G 9: 40,856,133 (GRCm39) Y118S possibly damaging Het
Kif23 C A 9: 61,844,046 (GRCm39) K154N possibly damaging Het
Krt79 T C 15: 101,846,442 (GRCm39) T169A probably damaging Het
Llgl2 A G 11: 115,740,827 (GRCm39) E443G probably damaging Het
Ltbp4 A T 7: 27,023,587 (GRCm39) C786S probably damaging Het
Mme A T 3: 63,247,550 (GRCm39) E278D probably benign Het
Nt5c2 G T 19: 46,887,317 (GRCm39) Q162K probably benign Het
Obscn A G 11: 58,888,887 (GRCm39) V2109A possibly damaging Het
Or5p64 GGTAG GG 7: 107,855,229 (GRCm39) probably benign Het
Or8k28 T C 2: 86,285,704 (GRCm39) T304A probably benign Het
Or8k38 T A 2: 86,488,209 (GRCm39) I198L probably benign Het
Osmr G T 15: 6,881,921 (GRCm39) N74K probably benign Het
Pes1 AGAGGAGGAGGAGGAGGA AGAGGAGGAGGAGGA 11: 3,927,636 (GRCm39) probably benign Het
Pgd A T 4: 149,238,768 (GRCm39) probably null Het
Pld1 T A 3: 28,178,724 (GRCm39) S873T probably damaging Het
Polk A T 13: 96,620,272 (GRCm39) C664S probably benign Het
Proca1 A C 11: 78,095,773 (GRCm39) H135P probably benign Het
Ptgs2 A C 1: 149,980,061 (GRCm39) D333A probably damaging Het
Rexo5 T C 7: 119,423,035 (GRCm39) V289A probably damaging Het
Rnf125 T A 18: 21,112,117 (GRCm39) C49* probably null Het
Rprd2 C A 3: 95,673,216 (GRCm39) R729L probably damaging Het
Sipa1l1 A G 12: 82,388,994 (GRCm39) S407G possibly damaging Het
Slc35a4 C A 18: 36,815,834 (GRCm39) N221K probably damaging Het
Sorcs1 T C 19: 50,220,761 (GRCm39) D563G probably benign Het
Stk39 G T 2: 68,222,515 (GRCm39) T183K probably damaging Het
Tc2n T A 12: 101,644,835 (GRCm39) K264* probably null Het
Trim23 C T 13: 104,324,635 (GRCm39) R238W probably damaging Het
Ttn T C 2: 76,584,389 (GRCm39) T13913A probably damaging Het
Ubap1 T G 4: 41,379,832 (GRCm39) C349G probably damaging Het
Vmn1r170 A T 7: 23,305,759 (GRCm39) I54F possibly damaging Het
Other mutations in Trim66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01539:Trim66 APN 7 109,054,273 (GRCm39) missense probably benign 0.02
IGL01758:Trim66 APN 7 109,085,252 (GRCm39) critical splice donor site probably null
IGL01982:Trim66 APN 7 109,057,970 (GRCm39) missense probably benign 0.00
IGL01983:Trim66 APN 7 109,057,458 (GRCm39) nonsense probably null
IGL02149:Trim66 APN 7 109,060,109 (GRCm39) missense possibly damaging 0.66
IGL02392:Trim66 APN 7 109,059,481 (GRCm39) missense probably benign 0.01
IGL02483:Trim66 APN 7 109,076,837 (GRCm39) splice site probably benign
IGL02832:Trim66 APN 7 109,059,704 (GRCm39) missense probably damaging 1.00
IGL02945:Trim66 APN 7 109,059,383 (GRCm39) nonsense probably null
IGL03085:Trim66 APN 7 109,057,952 (GRCm39) missense probably benign 0.17
PIT1430001:Trim66 UTSW 7 109,074,454 (GRCm39) missense probably damaging 0.99
R0326:Trim66 UTSW 7 109,059,379 (GRCm39) missense probably benign 0.00
R0358:Trim66 UTSW 7 109,059,383 (GRCm39) nonsense probably null
R0401:Trim66 UTSW 7 109,074,471 (GRCm39) missense probably damaging 0.98
R0470:Trim66 UTSW 7 109,056,749 (GRCm39) splice site probably benign
R0568:Trim66 UTSW 7 109,059,902 (GRCm39) missense probably benign 0.00
R0669:Trim66 UTSW 7 109,054,199 (GRCm39) intron probably benign
R1015:Trim66 UTSW 7 109,054,440 (GRCm39) missense probably damaging 1.00
R1078:Trim66 UTSW 7 109,071,526 (GRCm39) missense probably damaging 1.00
R1099:Trim66 UTSW 7 109,074,661 (GRCm39) missense probably benign 0.34
R1181:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R1497:Trim66 UTSW 7 109,083,826 (GRCm39) missense probably benign 0.00
R1583:Trim66 UTSW 7 109,054,287 (GRCm39) missense probably damaging 1.00
R1843:Trim66 UTSW 7 109,075,046 (GRCm39) missense probably damaging 0.99
R1998:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R2016:Trim66 UTSW 7 109,071,439 (GRCm39) critical splice donor site probably null
R2143:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2144:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2145:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R3945:Trim66 UTSW 7 109,071,475 (GRCm39) missense possibly damaging 0.94
R4012:Trim66 UTSW 7 109,057,338 (GRCm39) missense probably damaging 0.98
R4464:Trim66 UTSW 7 109,076,897 (GRCm39) missense possibly damaging 0.51
R4473:Trim66 UTSW 7 109,081,202 (GRCm39) missense probably damaging 1.00
R4729:Trim66 UTSW 7 109,055,267 (GRCm39) critical splice donor site probably null
R4730:Trim66 UTSW 7 109,082,276 (GRCm39) missense probably damaging 1.00
R4775:Trim66 UTSW 7 109,056,796 (GRCm39) nonsense probably null
R4819:Trim66 UTSW 7 109,056,793 (GRCm39) missense probably damaging 1.00
R5269:Trim66 UTSW 7 109,056,797 (GRCm39) missense probably benign 0.00
R5557:Trim66 UTSW 7 109,082,944 (GRCm39) missense probably benign 0.06
R5832:Trim66 UTSW 7 109,054,409 (GRCm39) missense probably damaging 1.00
R6220:Trim66 UTSW 7 109,082,300 (GRCm39) missense probably damaging 0.97
R6243:Trim66 UTSW 7 109,059,481 (GRCm39) missense probably benign 0.01
R6374:Trim66 UTSW 7 109,085,269 (GRCm39) missense probably benign
R6450:Trim66 UTSW 7 109,059,945 (GRCm39) missense probably benign 0.09
R6543:Trim66 UTSW 7 109,075,086 (GRCm39) missense probably benign 0.01
R6788:Trim66 UTSW 7 109,076,961 (GRCm39) missense probably damaging 1.00
R6842:Trim66 UTSW 7 109,059,983 (GRCm39) missense probably benign 0.00
R7169:Trim66 UTSW 7 109,054,328 (GRCm39) missense probably benign 0.25
R7257:Trim66 UTSW 7 109,059,451 (GRCm39) missense probably damaging 1.00
R7328:Trim66 UTSW 7 109,056,958 (GRCm39) missense probably damaging 0.99
R7616:Trim66 UTSW 7 109,082,956 (GRCm39) missense probably damaging 0.99
R8423:Trim66 UTSW 7 109,074,599 (GRCm39) missense possibly damaging 0.77
R8855:Trim66 UTSW 7 109,081,188 (GRCm39) missense probably damaging 1.00
R9130:Trim66 UTSW 7 109,076,896 (GRCm39) missense possibly damaging 0.90
R9137:Trim66 UTSW 7 109,074,330 (GRCm39) missense probably damaging 0.99
R9640:Trim66 UTSW 7 109,074,825 (GRCm39) missense probably damaging 1.00
RF013:Trim66 UTSW 7 109,059,960 (GRCm39) missense probably damaging 0.99
RF024:Trim66 UTSW 7 109,059,947 (GRCm39) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- ACAGTTGACCAGTTATGAGCGCC -3'
(R):5'- AGCGAGAGACATCTCTTCCTCTTCC -3'

Sequencing Primer
(F):5'- AGTTATGAGCGCCTCCCC -3'
(R):5'- TCAGCACCTCCACTTACATC -3'
Posted On 2014-01-05