Incidental Mutation 'R1116:Nkg7'
ID 97271
Institutional Source Beutler Lab
Gene Symbol Nkg7
Ensembl Gene ENSMUSG00000004612
Gene Name natural killer cell group 7 sequence
Synonyms 2500004F03Rik
MMRRC Submission 039189-MU
Accession Numbers

Genbank: NM_024253.4; Ensembl: ENSMUST00000070518

Is this an essential gene? Non essential (E-score: 0.000) question?
Stock # R1116 (G1)
Quality Score 225
Status Validated
Chromosome 7
Chromosomal Location 43437073-43438249 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) G to A at 43437454 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Valine to Isoleucine at position 51 (V51I)
Ref Sequence ENSEMBL: ENSMUSP00000068946 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000004732] [ENSMUST00000040227] [ENSMUST00000070518] [ENSMUST00000177375] [ENSMUST00000206741] [ENSMUST00000206839]
AlphaFold no structure available at present
Predicted Effect probably benign
Transcript: ENSMUST00000004732
SMART Domains Protein: ENSMUSP00000004732
Gene: ENSMUSG00000093639

DomainStartEndE-ValueType
Pfam:PMP22_Claudin 1 157 5.5e-44 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000040227
SMART Domains Protein: ENSMUSP00000037980
Gene: ENSMUSG00000038973

DomainStartEndE-ValueType
Pfam:PMP22_Claudin 6 147 2.7e-19 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000070518
AA Change: V51I

PolyPhen 2 Score 0.090 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000068946
Gene: ENSMUSG00000004612
AA Change: V51I

DomainStartEndE-ValueType
Pfam:PMP22_Claudin 6 150 5e-14 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000177375
SMART Domains Protein: ENSMUSP00000135834
Gene: ENSMUSG00000093639

DomainStartEndE-ValueType
low complexity region 26 46 N/A INTRINSIC
low complexity region 116 131 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000206251
Predicted Effect probably benign
Transcript: ENSMUST00000206741
AA Change: V51I

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)
Predicted Effect probably benign
Transcript: ENSMUST00000206839
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.3%
  • 10x: 96.1%
  • 20x: 92.8%
Validation Efficiency 100% (49/49)
Allele List at MGI

All alleles(1) : Targeted, knock-out(1)

Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9230019H11Rik A T 10: 3,120,180 noncoding transcript Het
Abi3bp T C 16: 56,686,429 probably benign Het
Acacb C T 5: 114,210,956 P1028S probably damaging Het
Acad10 A G 5: 121,630,751 F717S probably damaging Het
Adgrg6 A T 10: 14,438,428 Y705N probably benign Het
Adm A G 7: 110,628,294 I6V probably benign Het
Agps T G 2: 75,861,925 probably benign Het
Atr C T 9: 95,867,636 Q501* probably null Het
Cacna2d3 A G 14: 29,064,321 probably benign Het
Ccnt1 G A 15: 98,544,338 R350W probably damaging Het
Cfap74 G A 4: 155,433,996 E564K probably benign Het
Clip1 T C 5: 123,579,491 E1250G probably damaging Het
Cryz A C 3: 154,621,603 probably benign Het
Dnah1 C A 14: 31,307,867 V494F probably benign Het
Dpep3 G T 8: 105,978,829 D96E probably damaging Het
Dyrk3 G A 1: 131,129,182 A418V probably damaging Het
Ehf T A 2: 103,267,009 N231I probably damaging Het
Eif4g3 T C 4: 138,091,775 probably null Het
Ergic3 G A 2: 156,016,787 V278M probably benign Het
Fabp3 C T 4: 130,312,387 T57I probably benign Het
Fam178b A T 1: 36,578,588 C82* probably null Het
Gm1647 C T 3: 69,156,872 Q31* probably null Het
Got1 T A 19: 43,502,974 K346* probably null Het
Grid2ip G A 5: 143,382,914 G656D possibly damaging Het
Grm2 A G 9: 106,647,927 Y530H probably damaging Het
Hyou1 T C 9: 44,384,681 I381T probably damaging Het
Kirrel C T 3: 87,089,151 M380I probably null Het
March11 T C 15: 26,409,295 L360P probably damaging Het
Mettl17 T C 14: 51,889,598 V281A probably benign Het
Micu2 A T 14: 57,954,200 D131E probably benign Het
Mug1 G C 6: 121,870,645 V661L probably benign Het
Myo18b A T 5: 112,803,279 D1488E probably damaging Het
Nlgn1 A C 3: 25,433,874 S766A probably benign Het
Olfr462 T A 11: 87,889,408 M163L probably benign Het
Otog T C 7: 46,300,601 probably benign Het
Pax2 T C 19: 44,757,424 S11P probably damaging Het
Pck2 A G 14: 55,545,366 D392G probably benign Het
Plxnd1 A T 6: 115,967,005 probably null Het
Prkdc A G 16: 15,783,079 D2868G probably benign Het
Prl3d2 T C 13: 27,126,002 L149P probably damaging Het
Purg A T 8: 33,386,745 H137L probably benign Het
Slc38a8 A T 8: 119,496,133 L150Q probably damaging Het
Tifab T A 13: 56,176,212 R139S possibly damaging Het
Txndc16 T C 14: 45,162,985 H353R probably benign Het
Upk2 A G 9: 44,453,789 probably null Het
Zdhhc25 G A 15: 88,600,620 V53I probably benign Het
Zfp738 T A 13: 67,670,243 probably null Het
Zfp810 C T 9: 22,279,085 E176K probably benign Het
Zfp846 T A 9: 20,593,263 W140R possibly damaging Het
Other mutations in Nkg7
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02997:Nkg7 APN 7 43437867 missense probably damaging 1.00
1mM(1):Nkg7 UTSW 7 43437401 missense probably damaging 1.00
R1288:Nkg7 UTSW 7 43437662 splice site probably null
R1467:Nkg7 UTSW 7 43437433 missense probably damaging 1.00
R1467:Nkg7 UTSW 7 43437433 missense probably damaging 1.00
R9500:Nkg7 UTSW 7 43437805 missense probably damaging 1.00
X0065:Nkg7 UTSW 7 43437591 missense possibly damaging 0.95
Predicted Primers PCR Primer
(F):5'- TGTCCCCTAAGAAGTGGAAACCCAG -3'
(R):5'- TCACCAGTCTAGGTGTCCCAACAG -3'

Sequencing Primer
(F):5'- TGGAAACCCAGAGCTGTGTC -3'
(R):5'- AGCCATGACAGTTGAGACC -3'
Posted On 2014-01-05