Mutagenetix > Incidental Mutation

Incidental Mutation 'R1118:Or4f14'

97505

Institutional Source

Beutler Lab

Gene Symbol

Or4f14

Ensembl Gene

ENSMUSG00000096566

Gene Name

olfactory receptor family 4 subfamily F member 14

Synonyms

Olfr1306, GA_x6K02T2Q125-72954873-72953935, MOR245-15

MMRRC Submission

039191-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R1118 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

111742335-111743273 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 111743222 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 18 (T18S)

Ref Sequence

ENSEMBL: ENSMUSP00000151142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099607] [ENSMUST00000214844]

AlphaFold

A2BFL7

Predicted Effect

probably benign
Transcript: ENSMUST00000099607
AA Change: T18S

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000097202
Gene: ENSMUSG00000096566
AA Change: T18S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	305	4.7e-43	PFAM
Pfam:7tm_1	41	287	9.7e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214844
AA Change: T18S

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.7%
20x: 94.3%

Validation Efficiency

98% (60/61)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apcdd1	A	G	18: 63,085,095 (GRCm39)	T431A	probably benign	Het
Bcan	T	A	3: 87,896,534 (GRCm39)	I721F	probably damaging	Het
Card10	T	C	15: 78,686,643 (GRCm39)	D58G	possibly damaging	Het
Cd200r2	A	G	16: 44,729,969 (GRCm39)	N171S	probably damaging	Het
Celsr1	T	C	15: 85,916,248 (GRCm39)	D575G	probably damaging	Het
Ces1f	A	G	8: 93,993,870 (GRCm39)		probably benign	Het
Cped1	A	T	6: 22,237,698 (GRCm39)	H938L	probably benign	Het
Creld1	A	G	6: 113,468,656 (GRCm39)	D259G	probably benign	Het
Cubn	T	C	2: 13,341,053 (GRCm39)	I2223V	possibly damaging	Het
Dop1a	A	G	9: 86,397,459 (GRCm39)	D921G	probably damaging	Het
Dusp7	T	C	9: 106,250,849 (GRCm39)	S325P	possibly damaging	Het
Fat4	A	T	3: 39,037,091 (GRCm39)	D3581V	possibly damaging	Het
Fhl3	T	C	4: 124,599,584 (GRCm39)		probably null	Het
Gap43	T	C	16: 42,112,167 (GRCm39)	E198G	probably benign	Het
Garin4	G	A	1: 190,896,682 (GRCm39)		probably benign	Het
Grina	T	C	15: 76,132,779 (GRCm39)	F182S	probably damaging	Het
Gsk3b	T	C	16: 38,028,346 (GRCm39)		probably benign	Het
Gtf3c3	C	T	1: 54,456,937 (GRCm39)	A488T	probably damaging	Het
Haus6	A	G	4: 86,503,563 (GRCm39)		probably null	Het
Hmcn1	C	T	1: 150,494,679 (GRCm39)	A4137T	possibly damaging	Het
Itih4	C	A	14: 30,618,124 (GRCm39)		probably benign	Het
Itprid1	T	C	6: 55,866,155 (GRCm39)	F183L	probably damaging	Het
Kif22	A	G	7: 126,631,916 (GRCm39)	S384P	probably benign	Het
Lbr	A	C	1: 181,648,233 (GRCm39)		probably benign	Het
Mei1	G	A	15: 82,000,068 (GRCm39)		probably benign	Het
Misp	T	C	10: 79,662,969 (GRCm39)	V462A	probably benign	Het
Mrgpra3	A	C	7: 47,239,039 (GRCm39)	L296V	possibly damaging	Het
Ndufa9	A	T	6: 126,799,031 (GRCm39)	L362Q	probably damaging	Het
Nlrp9c	A	T	7: 26,083,862 (GRCm39)	D572E	probably benign	Het
Otud4	C	T	8: 80,379,980 (GRCm39)		probably benign	Het
P4ha3	T	C	7: 99,962,535 (GRCm39)	I431T	probably damaging	Het
Pcdhb15	T	A	18: 37,606,815 (GRCm39)	F16I	probably benign	Het
Pcnp	A	G	16: 55,844,754 (GRCm39)	S49P	probably damaging	Het
Pdxdc1	T	A	16: 13,697,278 (GRCm39)		probably benign	Het
Pgc	T	A	17: 48,039,828 (GRCm39)		probably null	Het
Phf11a	T	C	14: 59,521,778 (GRCm39)	D131G	probably benign	Het
Prdm2	G	A	4: 142,858,953 (GRCm39)	H1446Y	possibly damaging	Het
Rad54b	T	C	4: 11,563,352 (GRCm39)	S4P	probably damaging	Het
Slc52a2	T	C	15: 76,423,808 (GRCm39)		probably benign	Het
Slc9a4	G	A	1: 40,623,490 (GRCm39)		probably benign	Het
Smad4	T	C	18: 73,773,333 (GRCm39)	D551G	probably benign	Het
Smg7	A	T	1: 152,742,326 (GRCm39)		probably benign	Het
Sned1	G	A	1: 93,209,376 (GRCm39)	V830M	possibly damaging	Het
Sspo	A	G	6: 48,436,352 (GRCm39)	Y1234C	probably damaging	Het
Stab2	A	T	10: 86,721,582 (GRCm39)		probably null	Het
Stk36	A	G	1: 74,671,925 (GRCm39)	E875G	probably benign	Het
Stmn4	T	G	14: 66,591,844 (GRCm39)		probably benign	Het
Tagln3	C	A	16: 45,544,635 (GRCm39)	R12L	probably damaging	Het
Tex14	A	T	11: 87,413,343 (GRCm39)	R1031S	probably benign	Het
Tia1	G	A	6: 86,396,091 (GRCm39)	V96I	probably benign	Het
Ticrr	C	T	7: 79,343,701 (GRCm39)	P1189S	probably benign	Het
Tnxb	G	A	17: 34,904,017 (GRCm39)	V1053M	probably damaging	Het
Tpp2	T	C	1: 44,031,556 (GRCm39)		probably null	Het
Trpm7	A	G	2: 126,664,406 (GRCm39)	M991T	possibly damaging	Het
Ttc3	T	A	16: 94,217,127 (GRCm39)		probably benign	Het
Vcan	G	A	13: 89,853,782 (GRCm39)	P393S	probably damaging	Het
Vmn2r107	G	A	17: 20,576,860 (GRCm39)	R286Q	probably benign	Het
Wdr87-ps	G	A	7: 29,233,669 (GRCm39)		noncoding transcript	Het
Wrap73	A	T	4: 154,236,884 (GRCm39)		probably null	Het
Zfp958	A	T	8: 4,676,169 (GRCm39)	N46Y	possibly damaging	Het

Other mutations in Or4f14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Or4f14	APN	2	111,742,381 (GRCm39)	missense	possibly damaging	0.95
IGL01310:Or4f14	APN	2	111,742,652 (GRCm39)	missense	probably benign	0.34
IGL01893:Or4f14	APN	2	111,742,589 (GRCm39)	missense	possibly damaging	0.65
IGL02433:Or4f14	APN	2	111,742,762 (GRCm39)	missense	probably damaging	1.00
IGL03302:Or4f14	APN	2	111,743,167 (GRCm39)	missense	possibly damaging	0.61
R0544:Or4f14	UTSW	2	111,742,905 (GRCm39)	nonsense	probably null
R0674:Or4f14	UTSW	2	111,743,018 (GRCm39)	missense	probably benign	0.41
R1764:Or4f14	UTSW	2	111,742,526 (GRCm39)	missense	possibly damaging	0.93
R2915:Or4f14	UTSW	2	111,743,064 (GRCm39)	missense	probably damaging	1.00
R3976:Or4f14	UTSW	2	111,742,951 (GRCm39)	missense	possibly damaging	0.84
R4855:Or4f14	UTSW	2	111,742,444 (GRCm39)	missense	probably benign	0.41
R6475:Or4f14	UTSW	2	111,743,204 (GRCm39)	nonsense	probably null
R6513:Or4f14	UTSW	2	111,743,228 (GRCm39)	missense	possibly damaging	0.89
R6536:Or4f14	UTSW	2	111,743,119 (GRCm39)	missense	possibly damaging	0.94
R6748:Or4f14	UTSW	2	111,742,702 (GRCm39)	missense	possibly damaging	0.47
R6843:Or4f14	UTSW	2	111,743,260 (GRCm39)	missense	probably damaging	1.00
R7006:Or4f14	UTSW	2	111,742,601 (GRCm39)	missense	probably benign	0.16
R7169:Or4f14	UTSW	2	111,742,939 (GRCm39)	missense	possibly damaging	0.95
R7230:Or4f14	UTSW	2	111,742,906 (GRCm39)	missense	probably damaging	1.00
R7419:Or4f14	UTSW	2	111,742,435 (GRCm39)	missense	probably damaging	1.00
R7448:Or4f14	UTSW	2	111,742,637 (GRCm39)	missense	probably benign	0.00
R7753:Or4f14	UTSW	2	111,742,927 (GRCm39)	missense	probably benign	0.06
R7761:Or4f14	UTSW	2	111,743,222 (GRCm39)	missense	probably benign	0.02
R8330:Or4f14	UTSW	2	111,742,724 (GRCm39)	missense	probably benign	0.00
R8497:Or4f14	UTSW	2	111,742,964 (GRCm39)	missense	possibly damaging	0.82
R8942:Or4f14	UTSW	2	111,743,207 (GRCm39)	missense	probably benign	0.05
R9603:Or4f14	UTSW	2	111,743,128 (GRCm39)	missense	possibly damaging	0.69

Predicted Primers

PCR Primer
(F):5'- CGGGCTCATAATGGTCAGGTAATGG -3'
(R):5'- accccttcctttcctttcTCAATGC -3'

Sequencing Primer
(F):5'- TGGCTATGAGCAGCACCATC -3'
(R):5'- cctttcctttcTCAATGCTTCCC -3'

Posted On

2014-01-05