Mutagenetix > Incidental Mutation

Incidental Mutation 'R0011:Olfr965'

98983

Institutional Source

Beutler Lab

Gene Symbol

Olfr965

Ensembl Gene

ENSMUSG00000095839

Gene Name

olfactory receptor 965

Synonyms

GA_x6K02T2PVTD-33416730-33417668, MOR171-28

MMRRC Submission

038306-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R0011 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

39711353-39720614 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 39719627 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 133 (N133K)

Ref Sequence

ENSEMBL: ENSMUSP00000150401 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069342] [ENSMUST00000213335] [ENSMUST00000215164]

AlphaFold

Q7TRA7

Predicted Effect

probably benign
Transcript: ENSMUST00000069342
AA Change: N133K

PolyPhen 2 Score 0.265 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000069696
Gene: ENSMUSG00000095839
AA Change: N133K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.4e-47	PFAM
Pfam:7tm_1	41	290	4.2e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213335
AA Change: N133K

PolyPhen 2 Score 0.265 (Sensitivity: 0.91; Specificity: 0.88)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214875

Predicted Effect

probably benign
Transcript: ENSMUST00000215164
AA Change: N133K

PolyPhen 2 Score 0.265 (Sensitivity: 0.91; Specificity: 0.88)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.7%
20x: 95.9%

Validation Efficiency

100% (59/59)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430419D17Rik	T	A	7: 131,229,993 (GRCm38)	L389Q	probably damaging	Het
A930011G23Rik	T	C	5: 99,232,354 (GRCm38)	Y344C	probably damaging	Het
Alox15	A	G	11: 70,349,596 (GRCm38)	V253A	possibly damaging	Het
Ank3	A	G	10: 69,979,451 (GRCm38)		probably benign	Het
Art3	T	A	5: 92,403,612 (GRCm38)	Y17N	probably damaging	Het
Asic3	C	T	5: 24,417,492 (GRCm38)		probably benign	Het
Bach2	G	T	4: 32,244,655 (GRCm38)		probably benign	Het
Brip1	C	A	11: 86,186,998 (GRCm38)	K201N	possibly damaging	Het
Casc1	T	A	6: 145,179,055 (GRCm38)	M515L	probably damaging	Het
Ccdc88a	T	C	11: 29,374,364 (GRCm38)	F6S	probably damaging	Het
Celsr2	A	G	3: 108,413,402 (GRCm38)	I698T	probably benign	Het
Cenpf	A	G	1: 189,650,706 (GRCm38)	S2664P	probably benign	Het
Cfap54	A	T	10: 93,065,225 (GRCm38)	C156S	probably damaging	Het
Cops4	C	A	5: 100,527,981 (GRCm38)	Q28K	probably benign	Het
Cyb5a	T	A	18: 84,877,822 (GRCm38)		probably benign	Het
Diaph3	A	T	14: 86,866,408 (GRCm38)	C847S	probably damaging	Het
Dnah3	T	C	7: 120,019,701 (GRCm38)	K1648R	probably damaging	Het
Emilin2	C	T	17: 71,273,868 (GRCm38)	G621E	probably benign	Het
Enpp1	T	A	10: 24,670,002 (GRCm38)	K228*	probably null	Het
Epg5	T	C	18: 77,948,483 (GRCm38)	C132R	probably benign	Het
Epha7	G	A	4: 28,962,564 (GRCm38)	D961N	probably benign	Het
G6pc2	C	A	2: 69,226,565 (GRCm38)		probably benign	Het
Gm7361	C	T	5: 26,258,878 (GRCm38)		probably benign	Het
Grin2c	T	C	11: 115,255,750 (GRCm38)	Y476C	probably damaging	Het
Hnrnpul1	T	G	7: 25,742,915 (GRCm38)		probably benign	Het
Igf2bp1	T	C	11: 96,005,584 (GRCm38)	D17G	probably damaging	Het
Insrr	T	C	3: 87,809,616 (GRCm38)	C688R	possibly damaging	Het
Itgb2l	T	C	16: 96,427,661 (GRCm38)		probably benign	Het
Kidins220	T	A	12: 24,999,352 (GRCm38)	V322E	probably damaging	Het
Klk1	C	T	7: 44,229,535 (GRCm38)	T149I	probably benign	Het
Mbd3l1	A	G	9: 18,484,567 (GRCm38)		probably benign	Het
Miox	C	T	15: 89,336,274 (GRCm38)	L189F	possibly damaging	Het
Mrc1	T	C	2: 14,261,337 (GRCm38)		probably null	Het
Mtr	T	C	13: 12,238,052 (GRCm38)		probably benign	Het
Ncoa6	TGC	TGCGC	2: 155,408,291 (GRCm38)		probably null	Het
Npy4r	C	T	14: 34,146,723 (GRCm38)	V203M	probably damaging	Het
Pik3r4	C	A	9: 105,644,637 (GRCm38)	T134K	probably benign	Het
Rdh19	T	A	10: 127,856,911 (GRCm38)	L149Q	probably damaging	Het
Sema3e	C	T	5: 14,144,011 (GRCm38)	R85*	probably null	Het
Shtn1	A	G	19: 59,032,218 (GRCm38)	S191P	possibly damaging	Het
Slc39a11	A	T	11: 113,247,833 (GRCm38)	F279L	probably benign	Het
Slc4a1	T	C	11: 102,357,110 (GRCm38)	K353E	possibly damaging	Het
Slc6a18	A	T	13: 73,665,619 (GRCm38)	M515K	possibly damaging	Het
Snapc4	A	G	2: 26,364,813 (GRCm38)	I1225T	probably benign	Het
Spidr	A	T	16: 15,966,603 (GRCm38)	W534R	probably benign	Het
Tmem202	T	A	9: 59,524,801 (GRCm38)	N81I	probably benign	Het
Tnfrsf1b	T	G	4: 145,222,966 (GRCm38)	R297S	possibly damaging	Het
Trim55	A	G	3: 19,670,999 (GRCm38)	T227A	probably benign	Het
Trim58	A	T	11: 58,643,120 (GRCm38)	T167S	probably benign	Het
Trp53i11	A	T	2: 93,199,353 (GRCm38)		probably benign	Het
Ttn	T	C	2: 76,810,355 (GRCm38)	H5356R	probably damaging	Het
Tyrp1	C	T	4: 80,840,793 (GRCm38)	T301I	probably damaging	Het
Wdr17	A	T	8: 54,672,501 (GRCm38)	I448K	possibly damaging	Het
Wscd1	T	C	11: 71,788,828 (GRCm38)	V509A	probably damaging	Het
Zfp251	A	G	15: 76,854,554 (GRCm38)	V108A	probably benign	Het

Other mutations in Olfr965

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01705:Olfr965	APN	9	39,719,581 (GRCm38)	missense	possibly damaging	0.95
IGL02365:Olfr965	APN	9	39,720,100 (GRCm38)	missense	probably damaging	0.98
IGL02365:Olfr965	APN	9	39,719,674 (GRCm38)	missense	possibly damaging	0.60
IGL03062:Olfr965	APN	9	39,720,035 (GRCm38)	missense	probably benign	0.26
IGL03330:Olfr965	APN	9	39,719,488 (GRCm38)	missense	probably benign	0.08
R0462:Olfr965	UTSW	9	39,719,410 (GRCm38)	missense	probably benign	0.01
R1505:Olfr965	UTSW	9	39,719,478 (GRCm38)	missense	probably damaging	1.00
R1995:Olfr965	UTSW	9	39,719,413 (GRCm38)	missense	probably damaging	1.00
R2049:Olfr965	UTSW	9	39,720,115 (GRCm38)	missense	probably damaging	1.00
R2110:Olfr965	UTSW	9	39,719,722 (GRCm38)	missense	probably benign	0.30
R3817:Olfr965	UTSW	9	39,720,108 (GRCm38)	missense	possibly damaging	0.95
R4152:Olfr965	UTSW	9	39,720,000 (GRCm38)	missense	probably benign	0.10
R4153:Olfr965	UTSW	9	39,720,000 (GRCm38)	missense	probably benign	0.10
R4351:Olfr965	UTSW	9	39,719,569 (GRCm38)	missense	probably damaging	0.99
R4377:Olfr965	UTSW	9	39,719,807 (GRCm38)	missense	probably benign	0.04
R4667:Olfr965	UTSW	9	39,719,709 (GRCm38)	missense	probably benign	0.09
R5526:Olfr965	UTSW	9	39,719,596 (GRCm38)	missense	possibly damaging	0.95
R5816:Olfr965	UTSW	9	39,719,230 (GRCm38)	start codon destroyed	probably null	1.00
R7113:Olfr965	UTSW	9	39,719,677 (GRCm38)	missense	probably benign
R7336:Olfr965	UTSW	9	39,719,610 (GRCm38)	missense	probably benign	0.28
R8153:Olfr965	UTSW	9	39,719,658 (GRCm38)	missense	possibly damaging	0.68
R8291:Olfr965	UTSW	9	39,719,545 (GRCm38)	missense	probably benign	0.00
R8779:Olfr965	UTSW	9	39,719,340 (GRCm38)	missense	probably damaging	0.99
R9617:Olfr965	UTSW	9	39,719,382 (GRCm38)	missense	possibly damaging	0.80
R9631:Olfr965	UTSW	9	39,719,865 (GRCm38)	missense	possibly damaging	0.78

Predicted Primers

PCR Primer
(F):5'- ATCTACTTGTTCACGGAGCTGGGG -3'
(R):5'- CTCAAGATTGTCAGCATTGGCACAC -3'

Sequencing Primer
(F):5'- TGGTCATCTTGATCTCGATTAGC -3'
(R):5'- CCTCATTGACAAATGTGCTGGAG -3'

Posted On

2014-01-10