Incidental Mutation 'R1214:Or4f4b'
ID 99512
Institutional Source Beutler Lab
Gene Symbol Or4f4b
Ensembl Gene ENSMUSG00000061195
Gene Name olfactory receptor family 4 subfamily F member 4B
Synonyms MOR245-6, GA_x6K02T2Q125-72534883-72535821, Olfr1289
MMRRC Submission 039283-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.154) question?
Stock # R1214 (G1)
Quality Score 225
Status Not validated
Chromosome 2
Chromosomal Location 111313777-111314673 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 111314237 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Serine at position 154 (F154S)
Ref Sequence ENSEMBL: ENSMUSP00000147119 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000102551] [ENSMUST00000104889] [ENSMUST00000120021] [ENSMUST00000207494] [ENSMUST00000214816] [ENSMUST00000217611]
AlphaFold A0A288CFY5
Predicted Effect probably damaging
Transcript: ENSMUST00000102551
AA Change: F154S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000099611
Gene: ENSMUSG00000108908
AA Change: F154S

DomainStartEndE-ValueType
Pfam:7tm_4 17 289 6e-45 PFAM
Pfam:7TM_GPCR_Srsx 20 288 1.4e-7 PFAM
Pfam:7tm_1 27 273 7e-24 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000104889
SMART Domains Protein: ENSMUSP00000100485
Gene: ENSMUSG00000044039

DomainStartEndE-ValueType
Pfam:7tm_4 31 304 1.3e-43 PFAM
Pfam:7tm_1 41 287 2.5e-20 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000120021
AA Change: F182S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect probably damaging
Transcript: ENSMUST00000207494
AA Change: F154S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect probably benign
Transcript: ENSMUST00000214816
Predicted Effect probably benign
Transcript: ENSMUST00000217611
Predicted Effect noncoding transcript
Transcript: ENSMUST00000225425
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.2%
  • 10x: 96.1%
  • 20x: 92.0%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 13 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alkbh2 C T 5: 114,262,287 (GRCm39) E148K probably damaging Het
Dcdc2c A T 12: 28,580,429 (GRCm39) Y201* probably null Het
Dock9 C T 14: 121,823,728 (GRCm39) V1395I probably benign Het
Dync1h1 G A 12: 110,602,943 (GRCm39) E2195K probably benign Het
Micall2 T C 5: 139,697,396 (GRCm39) S652G probably damaging Het
Or1j13 G T 2: 36,369,981 (GRCm39) H54N possibly damaging Het
Or2ad1 C T 13: 21,326,337 (GRCm39) V297I probably benign Het
Ptpre A T 7: 135,280,987 (GRCm39) S643C probably damaging Het
Slc26a5 T A 5: 22,019,981 (GRCm39) I530F probably damaging Het
Slfn5 A G 11: 82,850,917 (GRCm39) M405V probably benign Het
Ttc29 A G 8: 79,052,211 (GRCm39) Y364C probably damaging Het
Tut7 A G 13: 59,953,140 (GRCm39) V100A possibly damaging Het
Wasf3 G A 5: 146,407,098 (GRCm39) V473M probably damaging Het
Other mutations in Or4f4b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01149:Or4f4b APN 2 111,314,446 (GRCm39) missense probably damaging 1.00
IGL01682:Or4f4b APN 2 111,314,188 (GRCm39) missense probably damaging 1.00
IGL02028:Or4f4b APN 2 111,313,816 (GRCm39) missense probably benign 0.01
IGL02731:Or4f4b APN 2 111,313,873 (GRCm39) missense probably benign 0.00
IGL03035:Or4f4b APN 2 111,314,168 (GRCm39) missense probably benign 0.04
R1471:Or4f4b UTSW 2 111,314,351 (GRCm39) missense probably damaging 1.00
R1714:Or4f4b UTSW 2 111,314,008 (GRCm39) missense probably damaging 1.00
R2088:Or4f4b UTSW 2 111,314,623 (GRCm39) missense probably damaging 1.00
R2136:Or4f4b UTSW 2 111,313,961 (GRCm39) missense probably damaging 1.00
R2141:Or4f4b UTSW 2 111,313,975 (GRCm39) missense probably benign 0.23
R3945:Or4f4b UTSW 2 111,314,032 (GRCm39) nonsense probably null
R4276:Or4f4b UTSW 2 111,313,849 (GRCm39) missense probably damaging 1.00
R4562:Or4f4b UTSW 2 111,313,909 (GRCm39) missense probably benign 0.00
R4896:Or4f4b UTSW 2 111,314,005 (GRCm39) missense possibly damaging 0.82
R4946:Or4f4b UTSW 2 111,314,311 (GRCm39) missense possibly damaging 0.93
R5004:Or4f4b UTSW 2 111,314,005 (GRCm39) missense possibly damaging 0.82
R5686:Or4f4b UTSW 2 111,314,488 (GRCm39) missense probably damaging 1.00
R6032:Or4f4b UTSW 2 111,314,195 (GRCm39) missense probably damaging 1.00
R6032:Or4f4b UTSW 2 111,314,195 (GRCm39) missense probably damaging 1.00
R6960:Or4f4b UTSW 2 111,314,071 (GRCm39) missense possibly damaging 0.70
R7293:Or4f4b UTSW 2 111,313,699 (GRCm39) splice site probably null
R7642:Or4f4b UTSW 2 111,313,823 (GRCm39) missense probably damaging 0.96
R8429:Or4f4b UTSW 2 111,313,840 (GRCm39) missense possibly damaging 0.55
R8447:Or4f4b UTSW 2 111,314,101 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GTGTCCTCTGTCACAGCTCCAAAG -3'
(R):5'- TAGATGACCTATCAGAAGGGCGGC -3'

Sequencing Primer
(F):5'- GCAAAGTCATCTCTGTTAAGGGC -3'
(R):5'- CACTGTTGGCAATGACCATG -3'
Posted On 2014-01-15