Incidental Mutation 'R1174:Tdo2'
ID99785
Institutional Source Beutler Lab
Gene Symbol Tdo2
Ensembl Gene ENSMUSG00000028011
Gene Nametryptophan 2,3-dioxygenase
Synonymschky, TO, TDO
MMRRC Submission 039247-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.220) question?
Stock #R1174 (G1)
Quality Score225
Status Not validated
Chromosome3
Chromosomal Location81957090-81976202 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 81974376 bp
ZygosityHeterozygous
Amino Acid Change Serine to Arginine at position 40 (S40R)
Ref Sequence ENSEMBL: ENSMUSP00000141237 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029645] [ENSMUST00000193879]
Predicted Effect probably damaging
Transcript: ENSMUST00000029645
AA Change: S59R

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000029645
Gene: ENSMUSG00000028011
AA Change: S59R

DomainStartEndE-ValueType
Pfam:Trp_dioxygenase 26 372 8e-177 PFAM
low complexity region 393 406 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000193879
AA Change: S40R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000141237
Gene: ENSMUSG00000028011
AA Change: S40R

DomainStartEndE-ValueType
Pfam:Trp_dioxygenase 7 353 1.4e-174 PFAM
low complexity region 374 387 N/A INTRINSIC
Meta Mutation Damage Score 0.3679 question?
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.1%
  • 10x: 95.4%
  • 20x: 89.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased plasma and brain levels of tryptophan, increased serotonin levels in the brain, decreased anxiety-related behavior, increased neuronal precursor proliferation and accelerated neurogenesis in the granule cell layer of the olfactory bulb. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2610318N02Rik T A 16: 17,117,582 H214L probably benign Het
4921507P07Rik T A 6: 50,589,141 K118M probably damaging Het
Actn3 A G 19: 4,864,756 L477P probably damaging Het
Adamts12 T C 15: 11,071,757 V129A probably benign Het
Agxt2 T C 15: 10,373,751 F81S probably damaging Het
Alpk1 T A 3: 127,680,810 S515C probably damaging Het
Bivm A G 1: 44,126,782 T131A probably benign Het
Borcs5 T A 6: 134,710,133 N150K probably damaging Het
Cdh7 G A 1: 110,061,132 V255I probably benign Het
Cox4i1 C A 8: 120,674,050 N151K probably benign Het
Cpeb4 T C 11: 31,920,472 L432P probably damaging Het
Dnajb11 T A 16: 22,870,673 D281E probably damaging Het
Eml6 A G 11: 29,749,824 S1771P possibly damaging Het
Fes A T 7: 80,377,951 I815N probably damaging Het
Ghrhr T A 6: 55,388,269 L416* probably null Het
Glipr1l2 C A 10: 112,083,466 L31I possibly damaging Het
Gm10093 A T 17: 78,492,078 Y166F probably benign Het
Gm1818 A G 12: 48,556,199 noncoding transcript Het
Gpr149 A G 3: 62,604,467 L37P probably damaging Het
Hoxa9 A G 6: 52,225,713 I131T probably damaging Het
Idh1 G T 1: 65,161,160 N348K probably benign Het
Klf6 A G 13: 5,861,712 D25G probably benign Het
Laptm4a T C 12: 8,936,716 V258A probably damaging Het
Lypd6b C T 2: 49,943,597 A83V possibly damaging Het
Map3k19 A G 1: 127,823,880 V578A probably benign Het
Mfsd13a T A 19: 46,374,686 I511N probably benign Het
Mrm3 T C 11: 76,250,024 V286A probably damaging Het
Muc6 C T 7: 141,648,101 G708S probably damaging Het
Neurl4 T C 11: 69,903,721 probably null Het
Pclo T C 5: 14,677,646 probably benign Het
Pcnt A T 10: 76,393,044 probably null Het
Pik3r4 G T 9: 105,663,174 G754C probably damaging Het
Qtrt1 A G 9: 21,412,486 T136A probably benign Het
Rgs12 T A 5: 34,966,465 C531S probably benign Het
Rin1 C A 19: 5,055,203 Q764K probably benign Het
Robo4 C T 9: 37,413,052 R959W probably damaging Het
Rxfp2 T C 5: 150,051,556 V210A probably benign Het
Serpinb9d C T 13: 33,200,625 P195L probably benign Het
Sos1 A C 17: 80,445,608 Y323* probably null Het
Ssxb8 T G X: 8,689,823 H88P probably damaging Het
Svs1 C A 6: 48,990,239 P707H probably damaging Het
Tas2r140 T C 6: 133,054,871 E308G probably benign Het
Tle4 C T 19: 14,468,262 V207I probably benign Het
Txlnb A G 10: 17,842,756 N445S probably benign Het
Ubap2l A T 3: 90,023,500 S413T probably benign Het
Vangl2 G T 1: 172,004,786 T501N probably damaging Het
Vmn2r12 A T 5: 109,092,854 I131N probably benign Het
Vwa1 C T 4: 155,773,266 G25D probably damaging Het
Zfp185 A T X: 72,999,323 E138D possibly damaging Het
Other mutations in Tdo2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02127:Tdo2 APN 3 81958925 missense probably damaging 0.99
IGL02129:Tdo2 APN 3 81958925 missense probably damaging 0.99
IGL02271:Tdo2 APN 3 81963917 splice site probably benign
IGL02686:Tdo2 APN 3 81968155 missense probably benign 0.00
IGL02802:Tdo2 APN 3 81975697 intron probably benign
IGL03171:Tdo2 APN 3 81967029 missense probably benign
IGL03285:Tdo2 APN 3 81958789 unclassified probably null
R0052:Tdo2 UTSW 3 81967025 missense probably benign 0.37
R0052:Tdo2 UTSW 3 81967025 missense probably benign 0.37
R0335:Tdo2 UTSW 3 81964000 missense probably benign
R0720:Tdo2 UTSW 3 81962758 missense probably damaging 1.00
R1175:Tdo2 UTSW 3 81974376 missense probably damaging 1.00
R1222:Tdo2 UTSW 3 81961468 unclassified probably null
R1418:Tdo2 UTSW 3 81961468 unclassified probably null
R1868:Tdo2 UTSW 3 81960546 missense probably benign 0.04
R1918:Tdo2 UTSW 3 81958940 missense probably damaging 1.00
R2031:Tdo2 UTSW 3 81969505 missense probably damaging 1.00
R2513:Tdo2 UTSW 3 81969505 missense possibly damaging 0.91
R3615:Tdo2 UTSW 3 81975428 missense possibly damaging 0.68
R3616:Tdo2 UTSW 3 81975428 missense possibly damaging 0.68
R3872:Tdo2 UTSW 3 81968086 missense probably benign 0.08
R5260:Tdo2 UTSW 3 81975323 critical splice donor site probably null
R5547:Tdo2 UTSW 3 81958940 missense probably damaging 1.00
R6029:Tdo2 UTSW 3 81961440 missense probably damaging 1.00
R6089:Tdo2 UTSW 3 81962728 missense probably damaging 1.00
R6163:Tdo2 UTSW 3 81975403 missense possibly damaging 0.49
R6379:Tdo2 UTSW 3 81958795 unclassified probably benign
R7060:Tdo2 UTSW 3 81969559 missense probably damaging 1.00
R7544:Tdo2 UTSW 3 81971635 critical splice donor site probably null
R7585:Tdo2 UTSW 3 81962758 missense probably damaging 1.00
R7724:Tdo2 UTSW 3 81968083 critical splice donor site probably null
Predicted Primers
Posted On2014-01-15