Incidental Mutation 'R1219:Slc1a1'
| ID |
99971 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Slc1a1
|
| Ensembl Gene |
ENSMUSG00000024935 |
| Gene Name |
solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 |
| Synonyms |
D130048G10Rik, EAAC1, MEAAC1, EAAT3 |
| MMRRC Submission |
039288-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R1219 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
19 |
| Chromosomal Location |
28812535-28891360 bp(+) (GRCm39) |
| Type of Mutation |
splice site |
| DNA Base Change (assembly) |
G to A
at 28882146 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000137486
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000025875]
[ENSMUST00000175647]
[ENSMUST00000179171]
|
| AlphaFold |
P51906 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000025875
|
| SMART Domains |
Protein: ENSMUSP00000025875
Gene: ENSMUSG00000024935
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:SDF
|
20 |
464 |
2.3e-135 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000160702
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000161119
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000162189
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000175647
|
| SMART Domains |
Protein: ENSMUSP00000135813
Gene: ENSMUSG00000064202
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:SPATA6
|
6 |
78 |
4.5e-22 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000179171
|
| SMART Domains |
Protein: ENSMUSP00000137486
Gene: ENSMUSG00000064202
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
36 |
58 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.6%
- 10x: 97.0%
- 20x: 94.8%
|
| Validation Efficiency |
98% (44/45) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]
PHENOTYPE: Mice homozygous for disruptions in this gene display reduced locomotor activity and excessive excretion of glutamate and aspartate. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 43 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Atad2 |
A |
G |
15: 57,998,307 (GRCm39) |
S22P |
probably benign |
Het |
| Atrn |
A |
T |
2: 130,862,927 (GRCm39) |
T1336S |
possibly damaging |
Het |
| Bltp1 |
T |
A |
3: 37,000,619 (GRCm39) |
L1266* |
probably null |
Het |
| Car9 |
G |
T |
4: 43,512,439 (GRCm39) |
|
probably null |
Het |
| Ccdc113 |
T |
C |
8: 96,264,895 (GRCm39) |
|
probably benign |
Het |
| Ccdc158 |
A |
G |
5: 92,802,040 (GRCm39) |
|
probably benign |
Het |
| Ciao3 |
T |
C |
17: 25,994,075 (GRCm39) |
I41T |
probably damaging |
Het |
| Dcun1d3 |
G |
T |
7: 119,458,631 (GRCm39) |
Q135K |
probably damaging |
Het |
| Dnah7b |
A |
C |
1: 46,379,280 (GRCm39) |
E3671D |
probably benign |
Het |
| Eea1 |
A |
G |
10: 95,846,623 (GRCm39) |
|
probably benign |
Het |
| Entrep3 |
T |
C |
3: 89,091,155 (GRCm39) |
V42A |
probably damaging |
Het |
| Gdf10 |
G |
A |
14: 33,654,710 (GRCm39) |
A406T |
probably benign |
Het |
| Gm5111 |
A |
G |
6: 48,567,328 (GRCm39) |
|
probably benign |
Het |
| Golga3 |
G |
T |
5: 110,332,215 (GRCm39) |
E50* |
probably null |
Het |
| Junb |
T |
C |
8: 85,704,268 (GRCm39) |
E264G |
probably damaging |
Het |
| Kash5 |
A |
G |
7: 44,838,832 (GRCm39) |
|
probably benign |
Het |
| Kifc1 |
G |
A |
17: 34,103,685 (GRCm39) |
R195C |
probably benign |
Het |
| Krt18 |
T |
C |
15: 101,939,723 (GRCm39) |
|
probably benign |
Het |
| Man1a |
G |
A |
10: 53,795,249 (GRCm39) |
|
probably benign |
Het |
| Mapkbp1 |
G |
T |
2: 119,849,831 (GRCm39) |
G768* |
probably null |
Het |
| Mybpc2 |
A |
C |
7: 44,165,458 (GRCm39) |
|
probably null |
Het |
| Nectin3 |
A |
T |
16: 46,275,042 (GRCm39) |
C238* |
probably null |
Het |
| Ntf3 |
G |
T |
6: 126,079,174 (GRCm39) |
R98S |
possibly damaging |
Het |
| Nup153 |
T |
G |
13: 46,840,695 (GRCm39) |
Q971P |
probably benign |
Het |
| Nup155 |
A |
G |
15: 8,146,822 (GRCm39) |
T221A |
possibly damaging |
Het |
| Ppp2r1b |
T |
C |
9: 50,778,621 (GRCm39) |
|
probably benign |
Het |
| Prkd1 |
A |
T |
12: 50,435,125 (GRCm39) |
V534E |
probably damaging |
Het |
| Rabep2 |
A |
G |
7: 126,028,799 (GRCm39) |
E26G |
probably damaging |
Het |
| Rnf213 |
C |
A |
11: 119,327,003 (GRCm39) |
N1663K |
probably damaging |
Het |
| Slc36a4 |
T |
C |
9: 15,634,832 (GRCm39) |
Y125H |
probably damaging |
Het |
| Slc6a11 |
G |
A |
6: 114,202,772 (GRCm39) |
|
probably benign |
Het |
| Stab1 |
C |
T |
14: 30,862,578 (GRCm39) |
|
probably null |
Het |
| Sumf2 |
G |
T |
5: 129,883,613 (GRCm39) |
A164S |
probably benign |
Het |
| Sv2b |
T |
C |
7: 74,786,160 (GRCm39) |
D420G |
probably benign |
Het |
| Ube2v1 |
T |
C |
2: 167,459,831 (GRCm39) |
D56G |
probably benign |
Het |
| Ung |
A |
G |
5: 114,270,228 (GRCm39) |
|
probably benign |
Het |
| Vcan |
T |
C |
13: 89,828,023 (GRCm39) |
Y2181C |
probably damaging |
Het |
| Vmn1r238 |
G |
A |
18: 3,123,135 (GRCm39) |
T93I |
possibly damaging |
Het |
| Vmn2r14 |
A |
C |
5: 109,372,440 (GRCm39) |
S17A |
probably benign |
Het |
| Vmn2r25 |
A |
G |
6: 123,816,282 (GRCm39) |
V433A |
probably benign |
Het |
| Zfp36l2 |
A |
G |
17: 84,495,070 (GRCm39) |
|
probably null |
Het |
| Zfp646 |
G |
A |
7: 127,482,292 (GRCm39) |
G1490S |
probably benign |
Het |
| Zfp839 |
A |
G |
12: 110,834,707 (GRCm39) |
D654G |
possibly damaging |
Het |
|
| Other mutations in Slc1a1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02170:Slc1a1
|
APN |
19 |
28,880,153 (GRCm39) |
missense |
possibly damaging |
0.66 |
|
IGL02726:Slc1a1
|
APN |
19 |
28,889,169 (GRCm39) |
missense |
probably benign |
0.04 |
|
IGL02865:Slc1a1
|
APN |
19 |
28,882,738 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0008:Slc1a1
|
UTSW |
19 |
28,878,884 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0008:Slc1a1
|
UTSW |
19 |
28,878,884 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0490:Slc1a1
|
UTSW |
19 |
28,874,931 (GRCm39) |
missense |
probably benign |
|
|
R1333:Slc1a1
|
UTSW |
19 |
28,812,611 (GRCm39) |
start gained |
probably benign |
|
|
R1623:Slc1a1
|
UTSW |
19 |
28,882,122 (GRCm39) |
missense |
probably benign |
0.09 |
|
R1669:Slc1a1
|
UTSW |
19 |
28,889,194 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1746:Slc1a1
|
UTSW |
19 |
28,871,869 (GRCm39) |
missense |
probably benign |
0.31 |
|
R2516:Slc1a1
|
UTSW |
19 |
28,870,312 (GRCm39) |
missense |
probably benign |
0.31 |
|
R4198:Slc1a1
|
UTSW |
19 |
28,878,852 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4199:Slc1a1
|
UTSW |
19 |
28,878,852 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4200:Slc1a1
|
UTSW |
19 |
28,878,852 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4432:Slc1a1
|
UTSW |
19 |
28,880,109 (GRCm39) |
missense |
probably benign |
0.21 |
|
R4744:Slc1a1
|
UTSW |
19 |
28,871,925 (GRCm39) |
missense |
probably benign |
|
|
R5110:Slc1a1
|
UTSW |
19 |
28,889,208 (GRCm39) |
missense |
probably benign |
0.14 |
|
R5341:Slc1a1
|
UTSW |
19 |
28,874,968 (GRCm39) |
missense |
probably benign |
|
|
R6136:Slc1a1
|
UTSW |
19 |
28,882,810 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6153:Slc1a1
|
UTSW |
19 |
28,886,935 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6640:Slc1a1
|
UTSW |
19 |
28,871,970 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7950:Slc1a1
|
UTSW |
19 |
28,889,161 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8182:Slc1a1
|
UTSW |
19 |
28,878,848 (GRCm39) |
missense |
probably benign |
0.07 |
|
R8534:Slc1a1
|
UTSW |
19 |
28,882,746 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8962:Slc1a1
|
UTSW |
19 |
28,886,869 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9222:Slc1a1
|
UTSW |
19 |
28,882,794 (GRCm39) |
missense |
probably benign |
0.12 |
|
R9383:Slc1a1
|
UTSW |
19 |
28,889,125 (GRCm39) |
missense |
probably benign |
0.01 |
|
R9513:Slc1a1
|
UTSW |
19 |
28,812,734 (GRCm39) |
critical splice donor site |
probably null |
|
|
R9655:Slc1a1
|
UTSW |
19 |
28,870,283 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9773:Slc1a1
|
UTSW |
19 |
28,870,283 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R9774:Slc1a1
|
UTSW |
19 |
28,870,283 (GRCm39) |
missense |
probably damaging |
0.98 |
|
RF020:Slc1a1
|
UTSW |
19 |
28,856,555 (GRCm39) |
critical splice donor site |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TAAGCAAGCCTTGACATCCCCATCG -3'
(R):5'- GAATCTTTCCTGGGTATCAGGCCAC -3'
Sequencing Primer
(F):5'- GACATCTGTTCGCTGTCAGAC -3'
(R):5'- GGTATCAGGCCACATGGAG -3'
|
| Posted On |
2014-01-15 |
Incidental Mutation