Mutagenetix > Incidental Mutation

Incidental Mutation 'R4041:Ifna13'

313873

Institutional Source

Beutler Lab

Gene Symbol

Ifna13

Ensembl Gene

ENSMUSG00000063376

Gene Name

interferon alpha 13

Synonyms

MMRRC Submission

040966-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R4041 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

88561878-88562696 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 88562228 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 132 (F132S)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105149]

AlphaFold

Q80SU4

Predicted Effect

probably benign
Transcript: ENSMUST00000072734
AA Change: F132S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000072517
Gene: ENSMUSG00000063376
AA Change: F132S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	2.2e-71	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000105149
AA Change: F132S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000100780
Gene: ENSMUSG00000063376
AA Change: F132S

Domain	Start	End	E-Value	Type
IFabd	50	167	2.2e-71	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000120871

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.1%
20x: 94.4%

Validation Efficiency

100% (29/29)

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atf5	T	C	7: 44,462,921 (GRCm39)	T68A	possibly damaging	Het
Cage1	A	G	13: 38,203,153 (GRCm39)	L637P	possibly damaging	Het
Cfl1	T	C	19: 5,542,556 (GRCm39)	I12T	probably benign	Het
Clcn1	A	G	6: 42,286,902 (GRCm39)	E653G	probably damaging	Het
Cntnap5a	C	A	1: 116,112,129 (GRCm39)	P473Q	probably benign	Het
Dnah7b	T	C	1: 46,120,655 (GRCm39)	S91P	probably benign	Het
Fcgbp	A	G	7: 27,813,404 (GRCm39)	Q2313R	probably benign	Het
Gpc5	A	T	14: 115,370,628 (GRCm39)	Q151L	probably damaging	Het
Hexim1	A	G	11: 103,007,932 (GRCm39)	E62G	probably benign	Het
Irgm2	A	G	11: 58,110,956 (GRCm39)	M228V	probably benign	Het
Klhl41	A	G	2: 69,501,054 (GRCm39)	M172V	probably benign	Het
Klk1	A	T	7: 43,878,986 (GRCm39)	N260I	probably damaging	Het
Krit1	G	C	5: 3,859,642 (GRCm39)	R110P	probably damaging	Het
Krt7	T	A	15: 101,321,161 (GRCm39)		probably null	Het
Lrrn4cl	T	A	19: 8,829,468 (GRCm39)	Y149N	probably damaging	Het
Ltbp3	G	T	19: 5,801,899 (GRCm39)	C739F	possibly damaging	Het
Mbnl2	C	T	14: 120,626,486 (GRCm39)	H239Y	probably damaging	Het
Obscn	G	A	11: 58,942,357 (GRCm39)	R4942*	probably null	Het
Or2bd2	T	C	7: 6,443,688 (GRCm39)	L263P	probably damaging	Het
R3hcc1	A	G	14: 69,944,111 (GRCm39)	S45P	probably damaging	Het
Ryr1	A	T	7: 28,785,356 (GRCm39)	H1778Q	possibly damaging	Het
Scyl2	T	A	10: 89,485,914 (GRCm39)	K558N	probably damaging	Het
Shank1	G	A	7: 43,991,586 (GRCm39)	V677M	unknown	Het
Slc39a5	T	C	10: 128,232,337 (GRCm39)	M488V	possibly damaging	Het
Styk1	A	T	6: 131,289,880 (GRCm39)		probably null	Het
Styxl2	T	C	1: 165,927,680 (GRCm39)	D644G	probably benign	Het
Usp32	A	G	11: 84,908,565 (GRCm39)	V1047A	probably benign	Het
Wnt3a	G	T	11: 59,140,470 (GRCm39)	H349N	probably damaging	Het
Zbtb39	T	C	10: 127,579,423 (GRCm39)	C666R	probably damaging	Het
Zfp420	T	C	7: 29,575,427 (GRCm39)	M549T	probably benign	Het

Other mutations in Ifna13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02748:Ifna13	APN	4	88,562,097 (GRCm39)	missense	probably damaging	0.99
R1691:Ifna13	UTSW	4	88,562,291 (GRCm39)	missense	probably benign	0.00
R1938:Ifna13	UTSW	4	88,562,412 (GRCm39)	missense	probably damaging	0.98
R1986:Ifna13	UTSW	4	88,562,588 (GRCm39)	missense	probably benign	0.00
R2279:Ifna13	UTSW	4	88,562,156 (GRCm39)	missense	probably benign	0.27
R2435:Ifna13	UTSW	4	88,562,366 (GRCm39)	missense	probably damaging	1.00
R2445:Ifna13	UTSW	4	88,562,133 (GRCm39)	missense	probably damaging	1.00
R4612:Ifna13	UTSW	4	88,562,113 (GRCm39)	missense	probably damaging	1.00
R6271:Ifna13	UTSW	4	88,562,082 (GRCm39)	missense	possibly damaging	0.79
R8783:Ifna13	UTSW	4	88,562,526 (GRCm39)	missense	probably damaging	1.00
R8833:Ifna13	UTSW	4	88,562,157 (GRCm39)	nonsense	probably null
R9433:Ifna13	UTSW	4	88,562,540 (GRCm39)	missense	probably benign	0.08
RF009:Ifna13	UTSW	4	88,562,145 (GRCm39)	missense	probably damaging	1.00
Z1177:Ifna13	UTSW	4	88,562,615 (GRCm39)	missense	probably benign	0.26

Predicted Primers

PCR Primer
(F):5'- AGAAGAGTCCTCTCCACACTTTG -3'
(R):5'- TGCCCAGCAGATCAAGAAGG -3'

Sequencing Primer
(F):5'- ACACTTTGTCTCAGGACTCAC -3'
(R):5'- CCAGCAGATCAAGAAGGCTCAAG -3'

Posted On

2015-04-30