Mutagenetix > Incidental Mutation

Incidental Mutation 'R5628:Vmn1r82'

441923

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r82

Ensembl Gene

ENSMUSG00000058132

Gene Name

vomeronasal 1 receptor 82

Synonyms

V1rg12

MMRRC Submission

043167-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R5628 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

12038732-12039646 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 12039205 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 41 (N41K)

Ref Sequence

ENSEMBL: ENSMUSP00000154524 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072801] [ENSMUST00000191002] [ENSMUST00000227672]

AlphaFold

A0A087WRV1

Predicted Effect

probably damaging
Transcript: ENSMUST00000072801
AA Change: N158K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000072580
Gene: ENSMUSG00000058132
AA Change: N158K

Domain	Start	End	E-Value	Type
Pfam:V1R	35	295	1.3e-28	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000191002
AA Change: N159K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000140782
Gene: ENSMUSG00000058132
AA Change: N159K

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:V1R	36	296	8.4e-27	PFAM
Pfam:7tm_1	39	290	2e-5	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000227672
AA Change: N41K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam25	A	G	8: 41,208,747 (GRCm39)	D671G	probably benign	Het
Ap3b1	A	G	13: 94,613,556 (GRCm39)	D685G	unknown	Het
Atp6v1h	G	A	1: 5,206,112 (GRCm39)	W358*	probably null	Het
Atr	T	A	9: 95,756,279 (GRCm39)	Y830*	probably null	Het
B3galnt2	A	T	13: 14,169,737 (GRCm39)		probably null	Het
Casz1	T	C	4: 149,030,553 (GRCm39)	Y1191H	probably damaging	Het
Cdc40	T	G	10: 40,727,049 (GRCm39)	E169D	probably benign	Het
Cep55	C	T	19: 38,058,396 (GRCm39)	Q330*	probably null	Het
Clcn1	T	C	6: 42,275,823 (GRCm39)	V315A	probably damaging	Het
Cmya5	A	G	13: 93,226,218 (GRCm39)	F2957L	probably damaging	Het
Dnah2	C	T	11: 69,349,746 (GRCm39)	R2399Q	probably benign	Het
Dync1li2	T	C	8: 105,147,224 (GRCm39)	N490S	possibly damaging	Het
Ephb3	T	C	16: 21,036,869 (GRCm39)	Y111H	probably damaging	Het
Fam186a	A	C	15: 99,839,628 (GRCm39)	H2205Q	possibly damaging	Het
Fat3	T	C	9: 15,877,392 (GRCm39)	Y3407C	probably damaging	Het
Fbxw8	A	G	5: 118,230,622 (GRCm39)	V393A	probably damaging	Het
Fnip1	A	T	11: 54,394,459 (GRCm39)	D965V	probably benign	Het
Gramd2a	T	C	9: 59,615,006 (GRCm39)	M3T	probably benign	Het
Kctd15	T	C	7: 34,339,720 (GRCm39)	D283G	probably damaging	Het
Kif9	C	T	9: 110,343,621 (GRCm39)	R547*	probably null	Het
Map4	A	G	9: 109,910,915 (GRCm39)	T245A	probably benign	Het
Mindy4	C	T	6: 55,237,579 (GRCm39)	L385F	probably damaging	Het
Myo7b	A	G	18: 32,107,240 (GRCm39)	C1252R	probably benign	Het
Myt1l	T	A	12: 29,861,620 (GRCm39)	I134N	unknown	Het
Or5m10b	T	A	2: 85,699,149 (GRCm39)	I71N	probably damaging	Het
Osgin2	T	A	4: 15,998,998 (GRCm39)	N208I	probably benign	Het
Polr2b	G	A	5: 77,461,063 (GRCm39)	V29M	probably damaging	Het
Prdm15	T	A	16: 97,600,823 (GRCm39)	M812L	probably damaging	Het
Prr36	TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC	TGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGCTGGTCTGTGGAAGAGCGGCTTTGC	8: 4,266,273 (GRCm39)		probably benign	Het
Rev3l	T	A	10: 39,698,963 (GRCm39)	N1153K	probably damaging	Het
Rnf17	A	G	14: 56,724,409 (GRCm39)		probably null	Het
Rusc2	C	T	4: 43,425,348 (GRCm39)	T1151M	probably damaging	Het
Scrib	T	C	15: 75,921,389 (GRCm39)	T30A	possibly damaging	Het
Sephs1	T	C	2: 4,894,018 (GRCm39)	I73T	probably benign	Het
Sf3b1	C	T	1: 55,037,334 (GRCm39)	A861T	probably benign	Het
Shq1	A	G	6: 100,607,964 (GRCm39)	W316R	probably damaging	Het
Slc26a5	T	A	5: 22,021,974 (GRCm39)	D484V	probably benign	Het
Smg1	C	T	7: 117,753,924 (GRCm39)		probably benign	Het
Stard5	T	C	7: 83,282,355 (GRCm39)	I56T	probably benign	Het
Szt2	A	G	4: 118,230,414 (GRCm39)	V2653A	unknown	Het
Tmem217	A	T	17: 29,745,430 (GRCm39)	I100N	probably damaging	Het
Trpm2	C	T	10: 77,748,470 (GRCm39)	R1400Q	probably benign	Het
Zfp236	T	C	18: 82,675,247 (GRCm39)	D367G	probably damaging	Het
Zfyve1	A	T	12: 83,621,663 (GRCm39)	V244E	probably benign	Het

Other mutations in Vmn1r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01509:Vmn1r82	APN	7	12,039,096 (GRCm39)	missense	probably damaging	0.99
IGL01955:Vmn1r82	APN	7	12,039,650 (GRCm39)	splice site	probably null
IGL02267:Vmn1r82	APN	7	12,039,273 (GRCm39)	missense	probably damaging	1.00
R0336:Vmn1r82	UTSW	7	12,039,248 (GRCm39)	missense	probably benign	0.26
R2373:Vmn1r82	UTSW	7	12,038,982 (GRCm39)	missense	probably damaging	0.99
R2849:Vmn1r82	UTSW	7	12,039,333 (GRCm39)	missense	probably damaging	1.00
R4621:Vmn1r82	UTSW	7	12,039,263 (GRCm39)	missense	possibly damaging	0.71
R6180:Vmn1r82	UTSW	7	12,039,012 (GRCm39)	missense	probably damaging	0.97
R6263:Vmn1r82	UTSW	7	12,039,461 (GRCm39)	missense	probably damaging	1.00
R6877:Vmn1r82	UTSW	7	12,039,290 (GRCm39)	missense	possibly damaging	0.79
R9551:Vmn1r82	UTSW	7	12,039,600 (GRCm39)	missense	possibly damaging	0.69
R9552:Vmn1r82	UTSW	7	12,039,600 (GRCm39)	missense	possibly damaging	0.69
R9792:Vmn1r82	UTSW	7	12,039,083 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- ACCTTTATAGAGTATCCCGGGG -3'
(R):5'- CACCATGGATGTACTGGACC -3'

Sequencing Primer
(F):5'- GGGGGTTTCACTATATACAACATGCC -3'
(R):5'- CATGGATGTACTGGACCTGCTTC -3'

Posted On

2016-11-08