Incidental Mutation 'R6663:Zfp668'
| ID |
526874 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp668
|
| Ensembl Gene |
ENSMUSG00000049728 |
| Gene Name |
zinc finger protein 668 |
| Synonyms |
E130018B19Rik |
| MMRRC Submission |
044783-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.147)
|
| Stock # |
R6663 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
7 |
| Chromosomal Location |
127462219-127476000 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to A
at 127466941 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Cysteine
at position 212
(R212C)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000101870
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000054415]
[ENSMUST00000106261]
[ENSMUST00000106262]
[ENSMUST00000106263]
[ENSMUST00000144721]
|
| AlphaFold |
Q8K2R5 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000054415
AA Change: R212C
PolyPhen 2
Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000056105
Gene: ENSMUSG00000049728
AA Change: R212C
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
22 |
44 |
7.67e-2 |
SMART |
|
ZnF_C2H2
|
84 |
106 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
112 |
134 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
140 |
162 |
2.36e-2 |
SMART |
|
ZnF_C2H2
|
168 |
190 |
1.98e-4 |
SMART |
|
ZnF_C2H2
|
196 |
218 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
224 |
246 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
252 |
274 |
5.21e-4 |
SMART |
|
ZnF_C2H2
|
280 |
302 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
308 |
330 |
5.59e-4 |
SMART |
|
ZnF_C2H2
|
336 |
358 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
364 |
386 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
392 |
414 |
5.5e-3 |
SMART |
|
low complexity region
|
428 |
470 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
516 |
538 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
544 |
566 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
572 |
594 |
5.59e-4 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000106261
AA Change: R212C
PolyPhen 2
Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000101868
Gene: ENSMUSG00000049728
AA Change: R212C
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
22 |
44 |
7.67e-2 |
SMART |
|
ZnF_C2H2
|
84 |
106 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
112 |
134 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
140 |
162 |
2.36e-2 |
SMART |
|
ZnF_C2H2
|
168 |
190 |
1.98e-4 |
SMART |
|
ZnF_C2H2
|
196 |
218 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
224 |
246 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
252 |
274 |
5.21e-4 |
SMART |
|
ZnF_C2H2
|
280 |
302 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
308 |
330 |
5.59e-4 |
SMART |
|
ZnF_C2H2
|
336 |
358 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
364 |
386 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
392 |
414 |
5.5e-3 |
SMART |
|
low complexity region
|
428 |
470 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
516 |
538 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
544 |
566 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
572 |
594 |
5.59e-4 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000106262
AA Change: R212C
PolyPhen 2
Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000101869
Gene: ENSMUSG00000049728
AA Change: R212C
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
22 |
44 |
7.67e-2 |
SMART |
|
ZnF_C2H2
|
84 |
106 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
112 |
134 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
140 |
162 |
2.36e-2 |
SMART |
|
ZnF_C2H2
|
168 |
190 |
1.98e-4 |
SMART |
|
ZnF_C2H2
|
196 |
218 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
224 |
246 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
252 |
274 |
5.21e-4 |
SMART |
|
ZnF_C2H2
|
280 |
302 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
308 |
330 |
5.59e-4 |
SMART |
|
ZnF_C2H2
|
336 |
358 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
364 |
386 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
392 |
414 |
5.5e-3 |
SMART |
|
low complexity region
|
428 |
470 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
516 |
538 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
544 |
566 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
572 |
594 |
5.59e-4 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000106263
AA Change: R212C
PolyPhen 2
Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000101870
Gene: ENSMUSG00000049728
AA Change: R212C
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
22 |
44 |
7.67e-2 |
SMART |
|
ZnF_C2H2
|
84 |
106 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
112 |
134 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
140 |
162 |
2.36e-2 |
SMART |
|
ZnF_C2H2
|
168 |
190 |
1.98e-4 |
SMART |
|
ZnF_C2H2
|
196 |
218 |
2.91e-2 |
SMART |
|
ZnF_C2H2
|
224 |
246 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
252 |
274 |
5.21e-4 |
SMART |
|
ZnF_C2H2
|
280 |
302 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
308 |
330 |
5.59e-4 |
SMART |
|
ZnF_C2H2
|
336 |
358 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
364 |
386 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
392 |
414 |
5.5e-3 |
SMART |
|
low complexity region
|
428 |
470 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
516 |
538 |
3.63e-3 |
SMART |
|
ZnF_C2H2
|
544 |
566 |
1.3e-4 |
SMART |
|
ZnF_C2H2
|
572 |
594 |
5.59e-4 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000144721
|
| SMART Domains |
Protein: ENSMUSP00000116730
Gene: ENSMUSG00000049728
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
22 |
44 |
7.67e-2 |
SMART |
|
ZnF_C2H2
|
84 |
106 |
9.44e-2 |
SMART |
|
| Meta Mutation Damage Score |
0.2719 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.5%
- 10x: 97.9%
- 20x: 94.0%
|
| Validation Efficiency |
100% (32/32) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 32 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Ankrd17 |
A |
G |
5: 90,411,923 (GRCm39) |
S1314P |
probably damaging |
Het |
| Btbd3 |
T |
A |
2: 138,121,003 (GRCm39) |
I59K |
probably benign |
Het |
| Clcn2 |
A |
G |
16: 20,521,995 (GRCm39) |
*865R |
probably null |
Het |
| Col22a1 |
T |
C |
15: 71,691,908 (GRCm39) |
Q749R |
unknown |
Het |
| Cpsf2 |
T |
C |
12: 101,965,852 (GRCm39) |
Y606H |
probably damaging |
Het |
| Csn1s1 |
T |
C |
5: 87,823,599 (GRCm39) |
V154A |
probably benign |
Het |
| Cux1 |
T |
A |
5: 136,514,701 (GRCm39) |
E23V |
probably damaging |
Het |
| Cyp2j9 |
C |
A |
4: 96,467,679 (GRCm39) |
W269L |
probably benign |
Het |
| Dbf4 |
T |
C |
5: 8,453,184 (GRCm39) |
M273V |
probably benign |
Het |
| Dnhd1 |
A |
G |
7: 105,334,899 (GRCm39) |
|
probably null |
Het |
| Fezf1 |
A |
G |
6: 23,247,527 (GRCm39) |
S183P |
probably damaging |
Het |
| Irx2 |
A |
G |
13: 72,777,248 (GRCm39) |
Y23C |
probably damaging |
Het |
| Itga1 |
A |
T |
13: 115,110,641 (GRCm39) |
N983K |
probably benign |
Het |
| Kifc1 |
A |
G |
17: 34,100,430 (GRCm39) |
|
probably benign |
Het |
| Lrrc14b |
T |
C |
13: 74,509,480 (GRCm39) |
N309S |
probably damaging |
Het |
| Lyst |
A |
T |
13: 13,838,701 (GRCm39) |
|
probably null |
Het |
| Map1b |
T |
C |
13: 99,566,530 (GRCm39) |
T2064A |
unknown |
Het |
| Mark3 |
T |
A |
12: 111,541,517 (GRCm39) |
N11K |
probably benign |
Het |
| Med6 |
T |
A |
12: 81,628,649 (GRCm39) |
D80V |
possibly damaging |
Het |
| Mfhas1 |
A |
G |
8: 36,056,272 (GRCm39) |
E249G |
probably damaging |
Het |
| Mroh2b |
A |
G |
15: 4,977,417 (GRCm39) |
I1256M |
probably benign |
Het |
| Nkx2-9 |
G |
T |
12: 56,658,723 (GRCm39) |
R164S |
probably benign |
Het |
| Or4p20 |
T |
A |
2: 88,253,694 (GRCm39) |
N225I |
probably benign |
Het |
| Phf10 |
T |
C |
17: 15,179,774 (GRCm39) |
D33G |
probably null |
Het |
| Plekha5 |
A |
G |
6: 140,523,016 (GRCm39) |
M719V |
probably damaging |
Het |
| Pln |
A |
G |
10: 53,219,792 (GRCm39) |
|
probably benign |
Het |
| Prg4 |
G |
A |
1: 150,330,852 (GRCm39) |
|
probably benign |
Het |
| Slc35b3 |
A |
G |
13: 39,138,112 (GRCm39) |
L99P |
probably damaging |
Het |
| Tbccd1 |
AT |
ATGT |
16: 22,652,778 (GRCm39) |
|
probably null |
Het |
| Ube2j1 |
C |
T |
4: 33,045,198 (GRCm39) |
S157L |
probably damaging |
Het |
| Zfp462 |
A |
G |
4: 55,008,933 (GRCm39) |
T300A |
possibly damaging |
Het |
| Zup1 |
A |
G |
10: 33,825,431 (GRCm39) |
M17T |
possibly damaging |
Het |
|
| Other mutations in Zfp668 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00944:Zfp668
|
APN |
7 |
127,467,079 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01063:Zfp668
|
APN |
7 |
127,465,454 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01909:Zfp668
|
APN |
7 |
127,465,994 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02404:Zfp668
|
APN |
7 |
127,465,532 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02543:Zfp668
|
APN |
7 |
127,467,494 (GRCm39) |
nonsense |
probably null |
|
|
IGL02946:Zfp668
|
APN |
7 |
127,465,690 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
R0078:Zfp668
|
UTSW |
7 |
127,467,210 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R1521:Zfp668
|
UTSW |
7 |
127,466,252 (GRCm39) |
missense |
probably benign |
0.09 |
|
R1775:Zfp668
|
UTSW |
7 |
127,465,778 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R1873:Zfp668
|
UTSW |
7 |
127,465,654 (GRCm39) |
splice site |
probably null |
|
|
R1875:Zfp668
|
UTSW |
7 |
127,465,654 (GRCm39) |
splice site |
probably null |
|
|
R2066:Zfp668
|
UTSW |
7 |
127,466,203 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R2068:Zfp668
|
UTSW |
7 |
127,465,837 (GRCm39) |
missense |
probably benign |
0.06 |
|
R2278:Zfp668
|
UTSW |
7 |
127,465,998 (GRCm39) |
missense |
probably benign |
0.05 |
|
R5516:Zfp668
|
UTSW |
7 |
127,466,318 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5569:Zfp668
|
UTSW |
7 |
127,466,995 (GRCm39) |
nonsense |
probably null |
|
|
R5875:Zfp668
|
UTSW |
7 |
127,465,474 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R8543:Zfp668
|
UTSW |
7 |
127,466,392 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8822:Zfp668
|
UTSW |
7 |
127,466,315 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8905:Zfp668
|
UTSW |
7 |
127,465,598 (GRCm39) |
missense |
probably benign |
0.04 |
|
R9191:Zfp668
|
UTSW |
7 |
127,465,658 (GRCm39) |
missense |
probably benign |
|
|
R9217:Zfp668
|
UTSW |
7 |
127,465,804 (GRCm39) |
nonsense |
probably null |
|
|
R9655:Zfp668
|
UTSW |
7 |
127,466,113 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
| Predicted Primers |
PCR Primer
(F):5'- CGACTCCAAATACTGCCTGG -3'
(R):5'- ACTAAAGATCCACCAGCGTG -3'
Sequencing Primer
(F):5'- AAAGCCTTGCCTGCTCTCAG -3'
(R):5'- CACCAGCGTGGGCACAC -3'
|
| Posted On |
2018-07-23 |
Incidental Mutation