Mutagenetix > Incidental Mutation

Incidental Mutation 'R1577:Or2t26'

171235

Institutional Source

Beutler Lab

Gene Symbol

Or2t26

Ensembl Gene

ENSMUSG00000050763

Gene Name

olfactory receptor family 2 subfamily T member 26

Synonyms

GA_x6K02T2QP88-6286247-6285294, Olfr1395, MOR277-1

MMRRC Submission

039615-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R1577 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

49039086-49040039 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 49040016 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 311 (C311S)

Ref Sequence

ENSEMBL: ENSMUSP00000150679 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059379] [ENSMUST00000203377] [ENSMUST00000214195]

AlphaFold

Q8VGD7

Predicted Effect

probably benign
Transcript: ENSMUST00000059379
AA Change: C311S

PolyPhen 2 Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000050142
Gene: ENSMUSG00000050763
AA Change: C311S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	1e-50	PFAM
Pfam:7TM_GPCR_Srsx	36	252	4.8e-6	PFAM
Pfam:7tm_1	42	291	1.9e-28	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203377
AA Change: C311S

PolyPhen 2 Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000145357
Gene: ENSMUSG00000050763
AA Change: C311S

Domain	Start	End	E-Value	Type
Pfam:7tm_1	1	219	1.9e-18	PFAM
Pfam:7tm_4	1	237	2.4e-38	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214195
AA Change: C311S

PolyPhen 2 Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)

Coding Region Coverage

1x: 98.9%
3x: 97.8%
10x: 94.6%
20x: 87.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb3	T	C	1: 25,133,264 (GRCm39)	N404S	possibly damaging	Het
Cep85	T	C	4: 133,879,599 (GRCm39)	E383G	probably damaging	Het
Chst4	A	T	8: 110,756,476 (GRCm39)	H379Q	probably benign	Het
Clca3b	A	T	3: 144,529,280 (GRCm39)	I798N	probably damaging	Het
Cldnd1	T	G	16: 58,553,016 (GRCm39)	L159R	possibly damaging	Het
Cntnap3	T	C	13: 64,906,104 (GRCm39)	E834G	probably damaging	Het
Col2a1	C	T	15: 97,877,083 (GRCm39)	R1065Q	probably damaging	Het
Dchs1	T	C	7: 105,415,162 (GRCm39)	D674G	probably damaging	Het
Dntt	A	G	19: 41,044,224 (GRCm39)	Y463C	probably damaging	Het
Egfr	A	G	11: 16,819,241 (GRCm39)	E257G	probably benign	Het
Eif4b	C	T	15: 101,998,336 (GRCm39)	R339*	probably null	Het
Fat1	C	T	8: 45,476,420 (GRCm39)	T1822M	probably benign	Het
Fgd3	T	C	13: 49,435,413 (GRCm39)	N282D	probably damaging	Het
Fmo4	T	C	1: 162,631,269 (GRCm39)	M233V	possibly damaging	Het
Gabbr2	T	A	4: 46,684,319 (GRCm39)	M652L	probably benign	Het
Gal3st2c	G	A	1: 93,934,650 (GRCm39)	V13M	probably damaging	Het
Gapvd1	C	T	2: 34,599,240 (GRCm39)	G686D	probably damaging	Het
Gdap1l1	T	G	2: 163,280,524 (GRCm39)	L20R	probably damaging	Het
Gm5800	A	T	14: 51,952,016 (GRCm39)	M82K	probably benign	Het
Grm6	T	A	11: 50,753,972 (GRCm39)	C759S	probably damaging	Het
Hs3st1	A	T	5: 39,772,393 (GRCm39)	D83E	probably benign	Het
Il12rb1	A	G	8: 71,263,250 (GRCm39)	D39G	probably damaging	Het
Ldhb	C	A	6: 142,438,324 (GRCm39)	K244N	possibly damaging	Het
Lypd6	C	T	2: 50,080,710 (GRCm39)	R133*	probably null	Het
Med13l	G	A	5: 118,859,457 (GRCm39)	G215S	probably damaging	Het
Ncoa1	T	C	12: 4,345,196 (GRCm39)	D606G	probably damaging	Het
Noc2l	C	T	4: 156,325,079 (GRCm39)	T151M	probably damaging	Het
Or10q3	T	C	19: 11,847,741 (GRCm39)	T280A	probably damaging	Het
Or2ag2	T	C	7: 106,485,217 (GRCm39)	K269R	probably benign	Het
Or2at4	T	C	7: 99,384,563 (GRCm39)	L71P	probably damaging	Het
Or5aq1b	T	C	2: 86,901,741 (GRCm39)	T246A	probably benign	Het
Ppm1l	G	A	3: 69,460,403 (GRCm39)	G327R	probably damaging	Het
Rapgef5	C	T	12: 117,558,911 (GRCm39)	A282V	probably benign	Het
Rnf139	T	C	15: 58,771,367 (GRCm39)	V464A	probably damaging	Het
Rprd2	C	T	3: 95,672,047 (GRCm39)	E1119K	probably damaging	Het
Sipa1l2	G	A	8: 126,219,001 (GRCm39)	T112I	probably benign	Het
Skint6	T	C	4: 113,005,720 (GRCm39)	T363A	possibly damaging	Het
Slc22a16	G	T	10: 40,479,811 (GRCm39)	E607*	probably null	Het
Slc24a2	T	C	4: 86,909,648 (GRCm39)	Y690C	probably damaging	Het
Slc25a23	T	C	17: 57,354,306 (GRCm39)	S115G	probably benign	Het
Slc4a3	T	C	1: 75,527,535 (GRCm39)	L168P	probably damaging	Het
Spats2	T	A	15: 99,076,333 (GRCm39)	I137N	possibly damaging	Het
Stard4	A	T	18: 33,338,151 (GRCm39)	V133D	probably damaging	Het
Syn3	A	G	10: 86,284,728 (GRCm39)		probably null	Het
Tfpi	A	G	2: 84,263,447 (GRCm39)	I305T	probably damaging	Het
Tmtc1	T	A	6: 148,314,318 (GRCm39)		probably null	Het
Tpcn1	A	C	5: 120,682,485 (GRCm39)	W508G	probably damaging	Het
Ubr5	A	T	15: 38,030,974 (GRCm39)	N406K	possibly damaging	Het
Xrcc1	T	A	7: 24,265,052 (GRCm39)	L118*	probably null	Het
Zbtb5	T	C	4: 44,995,129 (GRCm39)	Y85C	probably damaging	Het
Zfand4	C	A	6: 116,306,373 (GRCm39)	Y735*	probably null	Het
Zfp180	G	T	7: 23,805,333 (GRCm39)	C584F	probably damaging	Het

Other mutations in Or2t26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02413:Or2t26	APN	11	49,039,384 (GRCm39)	missense	probably damaging	1.00
IGL02946:Or2t26	APN	11	49,039,719 (GRCm39)	missense	probably damaging	1.00
R0030:Or2t26	UTSW	11	49,039,867 (GRCm39)	missense	possibly damaging	0.81
R1013:Or2t26	UTSW	11	49,039,977 (GRCm39)	missense	probably damaging	1.00
R1908:Or2t26	UTSW	11	49,039,101 (GRCm39)	missense	possibly damaging	0.95
R3715:Or2t26	UTSW	11	49,039,642 (GRCm39)	missense	probably damaging	0.99
R4386:Or2t26	UTSW	11	49,039,842 (GRCm39)	missense	probably damaging	1.00
R4496:Or2t26	UTSW	11	49,039,214 (GRCm39)	missense	possibly damaging	0.86
R5030:Or2t26	UTSW	11	49,039,188 (GRCm39)	missense	probably benign	0.01
R6044:Or2t26	UTSW	11	49,039,522 (GRCm39)	missense	probably benign	0.29
R6226:Or2t26	UTSW	11	49,039,660 (GRCm39)	missense	possibly damaging	0.81
R7177:Or2t26	UTSW	11	49,040,012 (GRCm39)	nonsense	probably null
R7282:Or2t26	UTSW	11	49,039,945 (GRCm39)	missense	probably damaging	1.00
R7391:Or2t26	UTSW	11	49,039,806 (GRCm39)	missense	probably damaging	1.00
R7411:Or2t26	UTSW	11	49,039,821 (GRCm39)	missense	probably benign	0.01
R7888:Or2t26	UTSW	11	49,039,266 (GRCm39)	missense	probably damaging	1.00
R8869:Or2t26	UTSW	11	49,039,483 (GRCm39)	missense	probably damaging	1.00
R8944:Or2t26	UTSW	11	49,039,266 (GRCm39)	missense	probably damaging	1.00
R9048:Or2t26	UTSW	11	49,039,830 (GRCm39)	missense	probably damaging	1.00
R9253:Or2t26	UTSW	11	49,039,822 (GRCm39)	missense	probably damaging	0.98
R9445:Or2t26	UTSW	11	49,039,879 (GRCm39)	missense	probably benign	0.25
R9752:Or2t26	UTSW	11	49,039,681 (GRCm39)	missense	probably benign	0.27

Predicted Primers

PCR Primer
(F):5'- GCCATGATCACATACATGAGGCCAC -3'
(R):5'- TCCATCCACAGTCTAAGGGAAAGGG -3'

Sequencing Primer
(F):5'- TACACCATGATCACGCCCAT -3'
(R):5'- tgtgagaccttttcaaaaatggac -3'

Posted On

2014-04-13