Mutagenetix > Incidental Mutation

Incidental Mutation 'R2000:Or10g6'

225843

Institutional Source

Beutler Lab

Gene Symbol

Or10g6

Ensembl Gene

ENSMUSG00000046678

Gene Name

olfactory receptor family 10 subfamily G member 6

Synonyms

MOR223-8, GA_x6K02T2PVTD-33720892-33721824, Olfr981

MMRRC Submission

040010-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R2000 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

39933691-39934623 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 39933985 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 99 (I99V)

Ref Sequence

ENSEMBL: ENSMUSP00000149436 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059859] [ENSMUST00000215956]

AlphaFold

Q8VEU2

Predicted Effect

probably benign
Transcript: ENSMUST00000059859
AA Change: I99V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000057952
Gene: ENSMUSG00000046678
AA Change: I99V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	304	1.4e-54	PFAM
Pfam:7tm_1	41	288	8.8e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215956
AA Change: I99V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219526

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000220338

Meta Mutation Damage Score

0.0653

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

98% (62/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atf2	T	C	2: 73,693,584 (GRCm39)		probably null	Het
Atrn	T	C	2: 130,777,508 (GRCm39)	Y186H	probably damaging	Het
Birc6	T	C	17: 74,911,614 (GRCm39)	V1528A	possibly damaging	Het
Brpf3	A	C	17: 29,040,531 (GRCm39)	E984A	probably benign	Het
Btnl9	T	C	11: 49,059,948 (GRCm39)	N600S	probably benign	Het
Camk1d	A	T	2: 5,366,836 (GRCm39)	Y126*	probably null	Het
Casp8ap2	T	A	4: 32,634,874 (GRCm39)	L136H	probably damaging	Het
Cd46	A	G	1: 194,760,012 (GRCm39)	I280T	probably benign	Het
Cip2a	A	T	16: 48,835,332 (GRCm39)	Q699L	probably damaging	Het
Crebbp	A	T	16: 3,902,116 (GRCm39)	H2374Q	probably damaging	Het
Dock3	A	G	9: 106,870,160 (GRCm39)		probably benign	Het
Dph7	T	A	2: 24,861,653 (GRCm39)	D355E	probably benign	Het
Dync1li1	C	A	9: 114,542,631 (GRCm39)	F264L	probably benign	Het
Fer1l6	G	A	15: 58,474,160 (GRCm39)		probably benign	Het
Flt3	T	C	5: 147,278,048 (GRCm39)	D842G	probably damaging	Het
Fosl1	A	G	19: 5,500,383 (GRCm39)		probably benign	Het
Gabrr2	T	A	4: 33,084,400 (GRCm39)	I162N	probably damaging	Het
Gata6	T	C	18: 11,054,113 (GRCm39)	F14S	probably benign	Het
Gm15446	T	C	5: 110,090,677 (GRCm39)	S310P	possibly damaging	Het
Gmppa	A	C	1: 75,418,172 (GRCm39)	D190A	probably damaging	Het
Gpr108	C	T	17: 57,543,712 (GRCm39)	G455S	probably benign	Het
Gstm1	A	G	3: 107,922,127 (GRCm39)	F170S	probably damaging	Het
Gvin-ps6	G	A	7: 106,022,438 (GRCm39)	S188L	probably benign	Het
Gzf1	T	C	2: 148,526,531 (GRCm39)	I334T	probably benign	Het
Hadh	A	T	3: 131,038,888 (GRCm39)	I156K	probably benign	Het
Htr5a	G	A	5: 28,055,887 (GRCm39)	V293M	possibly damaging	Het
Ice1	C	T	13: 70,750,546 (GRCm39)	V47M	possibly damaging	Het
Ifnl3	G	T	7: 28,222,354 (GRCm39)	A32S	possibly damaging	Het
Itsn2	T	A	12: 4,716,176 (GRCm39)	Y978*	probably null	Het
Kif1a	G	T	1: 92,982,051 (GRCm39)	T792N	probably damaging	Het
Lgals9	T	C	11: 78,863,996 (GRCm39)	N50D	probably benign	Het
Lrp2	A	G	2: 69,297,434 (GRCm39)	Y3176H	probably damaging	Het
Lvrn	A	T	18: 47,038,374 (GRCm39)	N976I	probably benign	Het
Magea10	A	T	X: 71,426,379 (GRCm39)	I205K	probably benign	Het
Myo1c	A	T	11: 75,561,405 (GRCm39)	M820L	probably damaging	Het
Neb	A	T	2: 52,102,982 (GRCm39)	C4222*	probably null	Het
Nkain2	T	A	10: 32,766,281 (GRCm39)		probably benign	Het
Or3a1b	A	G	11: 74,012,406 (GRCm39)	Y97C	probably benign	Het
Or5d16	G	A	2: 87,773,490 (GRCm39)	L161F	probably benign	Het
Parp4	C	T	14: 56,851,181 (GRCm39)	T728M	probably damaging	Het
Pnma8a	T	A	7: 16,694,964 (GRCm39)	V273D	probably benign	Het
Ppp1r9b	A	G	11: 94,887,446 (GRCm39)	E486G	probably damaging	Het
Pth2	C	A	7: 44,831,146 (GRCm39)	R98S	possibly damaging	Het
Ramacl	A	T	13: 67,056,214 (GRCm39)	N69I	possibly damaging	Het
Rif1	T	A	2: 51,971,310 (GRCm39)	F263I	probably damaging	Het
Rnf213	A	T	11: 119,326,848 (GRCm39)	I1613F	probably damaging	Het
Rsbn1l	G	A	5: 21,107,368 (GRCm39)	H549Y	probably damaging	Het
Rtl1	C	A	12: 109,560,321 (GRCm39)	W506L	probably damaging	Het
Slc4a4	C	T	5: 89,176,206 (GRCm39)	P59L	probably damaging	Het
Smarcad1	A	G	6: 65,050,200 (GRCm39)	E273G	probably damaging	Het
Tectb	C	G	19: 55,169,431 (GRCm39)		probably benign	Het
Ttf1	T	A	2: 28,955,197 (GRCm39)	L187Q	possibly damaging	Het
Ttn	A	T	2: 76,800,047 (GRCm39)	I387N	probably damaging	Het
Ufsp1	T	C	5: 137,293,166 (GRCm39)		probably null	Het
Uox	A	C	3: 146,316,154 (GRCm39)	K30Q	possibly damaging	Het
Ush1c	A	G	7: 45,870,857 (GRCm39)	S327P	probably damaging	Het
Wwc1	A	T	11: 35,767,374 (GRCm39)	L419Q	probably damaging	Het
Xrn1	C	T	9: 95,927,616 (GRCm39)	Q1463*	probably null	Het
Yeats2	G	A	16: 20,005,141 (GRCm39)	A393T	probably benign	Het
Zfp101	C	T	17: 33,600,491 (GRCm39)	A422T	possibly damaging	Het

Other mutations in Or10g6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01481:Or10g6	APN	9	39,934,574 (GRCm39)	missense	possibly damaging	0.61
IGL02089:Or10g6	APN	9	39,934,066 (GRCm39)	missense	probably damaging	1.00
R0722:Or10g6	UTSW	9	39,934,295 (GRCm39)	missense	probably damaging	1.00
R1117:Or10g6	UTSW	9	39,934,058 (GRCm39)	missense	probably damaging	0.99
R1781:Or10g6	UTSW	9	39,934,541 (GRCm39)	missense	probably damaging	1.00
R1874:Or10g6	UTSW	9	39,934,151 (GRCm39)	missense	possibly damaging	0.86
R1893:Or10g6	UTSW	9	39,934,270 (GRCm39)	missense	possibly damaging	0.64
R1942:Or10g6	UTSW	9	39,934,048 (GRCm39)	missense	probably damaging	1.00
R1942:Or10g6	UTSW	9	39,934,031 (GRCm39)	missense	probably damaging	1.00
R1999:Or10g6	UTSW	9	39,933,985 (GRCm39)	missense	probably benign
R2173:Or10g6	UTSW	9	39,934,550 (GRCm39)	missense	probably damaging	0.97
R2760:Or10g6	UTSW	9	39,933,692 (GRCm39)	start codon destroyed	probably null	0.00
R4300:Or10g6	UTSW	9	39,934,435 (GRCm39)	missense	probably benign	0.02
R4530:Or10g6	UTSW	9	39,934,589 (GRCm39)	missense	probably benign
R4614:Or10g6	UTSW	9	39,934,255 (GRCm39)	missense	probably damaging	1.00
R4661:Or10g6	UTSW	9	39,933,823 (GRCm39)	missense	probably damaging	1.00
R4777:Or10g6	UTSW	9	39,933,994 (GRCm39)	missense	possibly damaging	0.90
R5017:Or10g6	UTSW	9	39,933,672 (GRCm39)	start gained	probably benign
R5065:Or10g6	UTSW	9	39,934,546 (GRCm39)	missense	probably benign	0.44
R5467:Or10g6	UTSW	9	39,933,733 (GRCm39)	missense	probably benign	0.00
R5668:Or10g6	UTSW	9	39,933,964 (GRCm39)	missense	probably damaging	1.00
R5687:Or10g6	UTSW	9	39,933,731 (GRCm39)	missense	probably damaging	1.00
R6432:Or10g6	UTSW	9	39,933,824 (GRCm39)	missense	probably damaging	1.00
R6620:Or10g6	UTSW	9	39,934,225 (GRCm39)	missense	probably damaging	1.00
R6992:Or10g6	UTSW	9	39,933,896 (GRCm39)	nonsense	probably null
R7156:Or10g6	UTSW	9	39,934,526 (GRCm39)	missense	probably benign	0.23
R7250:Or10g6	UTSW	9	39,934,050 (GRCm39)	nonsense	probably null
R7273:Or10g6	UTSW	9	39,933,961 (GRCm39)	missense	probably benign	0.04
R8006:Or10g6	UTSW	9	39,933,770 (GRCm39)	missense	probably damaging	1.00
R8701:Or10g6	UTSW	9	39,933,815 (GRCm39)	missense	probably damaging	1.00
R8835:Or10g6	UTSW	9	39,934,171 (GRCm39)	missense	possibly damaging	0.48
R9497:Or10g6	UTSW	9	39,934,616 (GRCm39)	missense	probably benign
R9733:Or10g6	UTSW	9	39,934,171 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AATGGGCTCATCATCCTCACTG -3'
(R):5'- GTAGTCAACTCGGTTTGGGC -3'

Sequencing Primer
(F):5'- CACTGTCTTGGTGGACATCCG -3'
(R):5'- CAGCCTGAATATGAAACTCGTTTGG -3'

Posted On

2014-08-25