Incidental Mutation 'R3038:Mrgpra1'
| ID |
264879 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mrgpra1
|
| Ensembl Gene |
ENSMUSG00000050650 |
| Gene Name |
MAS-related GPR, member A1 |
| Synonyms |
MrgA1 |
| MMRRC Submission |
040554-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.070)
|
| Stock # |
R3038 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
46984623-47003988 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
G to A
at 46984744 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamine to Stop codon
at position 312
(Q312*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000129978
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000098438]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably null
Transcript: ENSMUST00000098438
AA Change: Q312*
|
| SMART Domains |
Protein: ENSMUSP00000129978
Gene: ENSMUSG00000050650
AA Change: Q312*
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
14 |
27 |
N/A |
INTRINSIC |
|
low complexity region
|
36 |
49 |
N/A |
INTRINSIC |
|
Pfam:7tm_1
|
57 |
225 |
2.1e-8 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.7%
- 10x: 97.4%
- 20x: 95.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 17 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Bspry |
A |
G |
4: 62,415,220 (GRCm39) |
I468V |
probably benign |
Het |
| Cavin2 |
T |
A |
1: 51,340,416 (GRCm39) |
N364K |
possibly damaging |
Het |
| Ces1f |
T |
A |
8: 93,983,226 (GRCm39) |
N506I |
probably damaging |
Het |
| Dnhd1 |
A |
T |
7: 105,369,436 (GRCm39) |
Q4353L |
probably damaging |
Het |
| Dsc3 |
T |
C |
18: 20,124,617 (GRCm39) |
T36A |
possibly damaging |
Het |
| Hydin |
A |
G |
8: 111,309,321 (GRCm39) |
T4038A |
probably damaging |
Het |
| Kcnd3 |
C |
T |
3: 105,566,082 (GRCm39) |
A421V |
probably damaging |
Het |
| Kif1b |
T |
C |
4: 149,297,790 (GRCm39) |
I1083V |
probably benign |
Het |
| Lmo2 |
T |
C |
2: 103,811,407 (GRCm39) |
Y147H |
probably damaging |
Het |
| Pcdha1 |
T |
A |
18: 37,064,064 (GRCm39) |
F243I |
probably damaging |
Het |
| Ppp1r18 |
T |
C |
17: 36,179,274 (GRCm39) |
L383P |
probably damaging |
Het |
| Tmed9 |
A |
G |
13: 55,744,792 (GRCm39) |
K207E |
probably damaging |
Het |
| Tnfrsf19 |
A |
G |
14: 61,209,512 (GRCm39) |
S253P |
probably benign |
Het |
| Tspear |
T |
A |
10: 77,722,273 (GRCm39) |
Y624* |
probably null |
Het |
| Vmn2r16 |
G |
A |
5: 109,487,199 (GRCm39) |
C140Y |
probably damaging |
Het |
| Vwa7 |
T |
C |
17: 35,241,637 (GRCm39) |
V424A |
probably damaging |
Het |
| Zgpat |
TGGAGGAGGAGGAGGAGGA |
TGGAGGAGGAGGAGGA |
2: 181,007,811 (GRCm39) |
|
probably benign |
Het |
|
| Other mutations in Mrgpra1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00903:Mrgpra1
|
APN |
7 |
46,985,326 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01317:Mrgpra1
|
APN |
7 |
46,985,372 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01718:Mrgpra1
|
APN |
7 |
46,985,675 (GRCm39) |
splice site |
probably null |
|
|
IGL02252:Mrgpra1
|
APN |
7 |
46,984,912 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL03301:Mrgpra1
|
APN |
7 |
46,985,164 (GRCm39) |
missense |
probably benign |
|
|
R0012:Mrgpra1
|
UTSW |
7 |
46,985,218 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1019:Mrgpra1
|
UTSW |
7 |
46,984,833 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2224:Mrgpra1
|
UTSW |
7 |
46,984,854 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
R2520:Mrgpra1
|
UTSW |
7 |
46,985,020 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R2924:Mrgpra1
|
UTSW |
7 |
46,984,618 (GRCm39) |
splice site |
probably null |
|
|
R3900:Mrgpra1
|
UTSW |
7 |
46,985,275 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
R4692:Mrgpra1
|
UTSW |
7 |
46,985,446 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4783:Mrgpra1
|
UTSW |
7 |
46,985,218 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4784:Mrgpra1
|
UTSW |
7 |
46,985,218 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4785:Mrgpra1
|
UTSW |
7 |
46,985,218 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4981:Mrgpra1
|
UTSW |
7 |
46,984,959 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5031:Mrgpra1
|
UTSW |
7 |
46,984,985 (GRCm39) |
nonsense |
probably null |
|
|
R6760:Mrgpra1
|
UTSW |
7 |
46,984,789 (GRCm39) |
missense |
probably benign |
0.03 |
|
R7305:Mrgpra1
|
UTSW |
7 |
46,985,203 (GRCm39) |
missense |
probably benign |
0.38 |
|
R7348:Mrgpra1
|
UTSW |
7 |
46,985,157 (GRCm39) |
missense |
probably benign |
0.09 |
|
R7837:Mrgpra1
|
UTSW |
7 |
46,985,076 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R8158:Mrgpra1
|
UTSW |
7 |
46,985,204 (GRCm39) |
nonsense |
probably null |
|
|
R8856:Mrgpra1
|
UTSW |
7 |
46,985,583 (GRCm39) |
missense |
probably benign |
0.30 |
|
R9100:Mrgpra1
|
UTSW |
7 |
46,984,732 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R9694:Mrgpra1
|
UTSW |
7 |
46,985,268 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0012:Mrgpra1
|
UTSW |
7 |
46,985,623 (GRCm39) |
missense |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- AGGCATGCCAAGTCGGTAAG -3'
(R):5'- TGGGCAGATAAAGCTTACCAG -3'
Sequencing Primer
(F):5'- CATGCCAAGTCGGTAAGCTGAG -3'
(R):5'- GCATCCACTGGTTTCTGTTATTCAAG -3'
|
| Posted On |
2015-02-05 |
Incidental Mutation