Incidental Mutation 'R3961:Prss35'
| ID |
312087 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Prss35
|
| Ensembl Gene |
ENSMUSG00000033491 |
| Gene Name |
serine protease 35 |
| Synonyms |
6030424L22Rik |
| MMRRC Submission |
040836-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.099)
|
| Stock # |
R3961 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
9 |
| Chromosomal Location |
86625702-86640496 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 86637802 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Methionine to Valine
at position 191
(M191V)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000035271
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000036426]
[ENSMUST00000179574]
|
| AlphaFold |
Q8C0F9 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000036426
AA Change: M191V
PolyPhen 2
Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)
|
| SMART Domains |
Protein: ENSMUSP00000035271
Gene: ENSMUSG00000033491
AA Change: M191V
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
68 |
83 |
N/A |
INTRINSIC |
|
Tryp_SPc
|
132 |
399 |
4.13e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000179574
AA Change: M191V
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| SMART Domains |
Protein: ENSMUSP00000137445
Gene: ENSMUSG00000033491
AA Change: M191V
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
68 |
83 |
N/A |
INTRINSIC |
|
Tryp_SPc
|
132 |
399 |
4.13e-2 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.5%
- 10x: 97.0%
- 20x: 93.9%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 28 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 9930111J21Rik1 |
T |
C |
11: 48,838,803 (GRCm39) |
T595A |
possibly damaging |
Het |
| Bicral |
T |
C |
17: 47,135,751 (GRCm39) |
I486M |
probably damaging |
Het |
| Btbd1 |
C |
A |
7: 81,468,083 (GRCm39) |
E146* |
probably null |
Het |
| Cdcp1 |
T |
C |
9: 123,011,446 (GRCm39) |
T344A |
possibly damaging |
Het |
| Cenpm |
A |
T |
15: 82,118,574 (GRCm39) |
L180Q |
possibly damaging |
Het |
| Cers3 |
G |
T |
7: 66,435,823 (GRCm39) |
A261S |
probably benign |
Het |
| Dazl |
A |
G |
17: 50,595,161 (GRCm39) |
V91A |
probably damaging |
Het |
| Dsc2 |
C |
T |
18: 20,184,284 (GRCm39) |
V35I |
probably damaging |
Het |
| Fras1 |
T |
C |
5: 96,825,244 (GRCm39) |
|
probably null |
Het |
| Ltbp3 |
G |
A |
19: 5,804,050 (GRCm39) |
R854Q |
probably benign |
Het |
| Minar1 |
G |
A |
9: 89,483,963 (GRCm39) |
T478I |
probably damaging |
Het |
| Mme |
T |
A |
3: 63,252,613 (GRCm39) |
M419K |
probably damaging |
Het |
| Ncan |
G |
A |
8: 70,562,950 (GRCm39) |
T436M |
probably benign |
Het |
| Nphp3 |
G |
T |
9: 103,880,241 (GRCm39) |
E88* |
probably null |
Het |
| Or5ak23 |
T |
C |
2: 85,245,216 (GRCm39) |
I2M |
possibly damaging |
Het |
| Pdcl |
T |
C |
2: 37,242,199 (GRCm39) |
M184V |
probably benign |
Het |
| Polr3b |
T |
C |
10: 84,520,166 (GRCm39) |
M694T |
possibly damaging |
Het |
| Pramel12 |
T |
A |
4: 143,145,888 (GRCm39) |
N452K |
probably benign |
Het |
| Prkdc |
T |
G |
16: 15,647,475 (GRCm39) |
|
probably null |
Het |
| Rtn3 |
T |
C |
19: 7,435,510 (GRCm39) |
S142G |
probably damaging |
Het |
| Slc19a3 |
A |
T |
1: 83,000,678 (GRCm39) |
F113Y |
probably damaging |
Het |
| Taf7 |
A |
G |
18: 37,776,174 (GRCm39) |
V131A |
probably benign |
Het |
| Tesk1 |
A |
G |
4: 43,445,133 (GRCm39) |
|
probably null |
Het |
| Tmem131 |
C |
T |
1: 36,858,031 (GRCm39) |
D741N |
probably damaging |
Het |
| Tmem63a |
G |
A |
1: 180,790,679 (GRCm39) |
D446N |
possibly damaging |
Het |
| Tpte |
A |
G |
8: 22,849,431 (GRCm39) |
S553G |
probably damaging |
Het |
| Trpv3 |
A |
T |
11: 73,178,246 (GRCm39) |
K438* |
probably null |
Het |
| Vmn2r107 |
G |
A |
17: 20,595,717 (GRCm39) |
G757R |
probably damaging |
Het |
|
| Other mutations in Prss35 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01548:Prss35
|
APN |
9 |
86,637,327 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02749:Prss35
|
APN |
9 |
86,638,297 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0346:Prss35
|
UTSW |
9 |
86,637,404 (GRCm39) |
missense |
probably benign |
0.04 |
|
R0403:Prss35
|
UTSW |
9 |
86,638,090 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1664:Prss35
|
UTSW |
9 |
86,637,700 (GRCm39) |
missense |
probably benign |
0.29 |
|
R2016:Prss35
|
UTSW |
9 |
86,637,565 (GRCm39) |
missense |
probably benign |
0.37 |
|
R2017:Prss35
|
UTSW |
9 |
86,637,565 (GRCm39) |
missense |
probably benign |
0.37 |
|
R2325:Prss35
|
UTSW |
9 |
86,638,357 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2429:Prss35
|
UTSW |
9 |
86,637,398 (GRCm39) |
missense |
probably benign |
|
|
R2965:Prss35
|
UTSW |
9 |
86,637,635 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2966:Prss35
|
UTSW |
9 |
86,637,635 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4792:Prss35
|
UTSW |
9 |
86,637,722 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4902:Prss35
|
UTSW |
9 |
86,638,175 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6169:Prss35
|
UTSW |
9 |
86,637,491 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6446:Prss35
|
UTSW |
9 |
86,637,706 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6753:Prss35
|
UTSW |
9 |
86,638,153 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7008:Prss35
|
UTSW |
9 |
86,638,361 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7387:Prss35
|
UTSW |
9 |
86,637,974 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7523:Prss35
|
UTSW |
9 |
86,637,427 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7587:Prss35
|
UTSW |
9 |
86,637,427 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7652:Prss35
|
UTSW |
9 |
86,638,023 (GRCm39) |
missense |
probably benign |
|
|
R8013:Prss35
|
UTSW |
9 |
86,637,478 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8194:Prss35
|
UTSW |
9 |
86,637,666 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R8871:Prss35
|
UTSW |
9 |
86,637,244 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R9444:Prss35
|
UTSW |
9 |
86,638,157 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9462:Prss35
|
UTSW |
9 |
86,638,392 (GRCm39) |
missense |
|
|
|
R9695:Prss35
|
UTSW |
9 |
86,637,761 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CATCTTAGACAAAAGGTTCGCG -3'
(R):5'- TAGTCGTAGTCCAAGGCCAG -3'
Sequencing Primer
(F):5'- GCGACCAATTTCCCTTTTAATACAG -3'
(R):5'- CTCTTGACGCGGGTCCATTG -3'
|
| Posted On |
2015-04-29 |
Incidental Mutation