Mutagenetix > Incidental Mutation

Incidental Mutation 'R5220:Zdhhc25'

402286

Institutional Source

Beutler Lab

Gene Symbol

Zdhhc25

Ensembl Gene

ENSMUSG00000054117

Gene Name

zinc finger, DHHC domain containing 25

Synonyms

1700030J15Rik

MMRRC Submission

042793-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R5220 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

88484505-88485872 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 88485365 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 233 (Y233*)

Ref Sequence

ENSEMBL: ENSMUSP00000136289 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000066949]

AlphaFold

Q810M4

Predicted Effect

probably null
Transcript: ENSMUST00000066949
AA Change: Y233*

SMART Domains

Protein: ENSMUSP00000136289
Gene: ENSMUSG00000054117
AA Change: Y233*

Domain	Start	End	E-Value	Type
transmembrane domain	35	55	N/A	INTRINSIC
transmembrane domain	65	87	N/A	INTRINSIC
Pfam:zf-DHHC	105	235	2.4e-32	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.5%
20x: 95.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	A	G	7: 78,738,045 (GRCm39)	E250G	probably damaging	Het
Adgra1	A	G	7: 139,455,512 (GRCm39)	N380S	probably benign	Het
Ap3d1	G	A	10: 80,563,001 (GRCm39)	P160L	probably damaging	Het
Atg9a	C	T	1: 75,162,372 (GRCm39)	V505M	probably damaging	Het
Chsy3	A	T	18: 59,543,102 (GRCm39)	I747F	probably damaging	Het
Clec2g	A	G	6: 128,958,269 (GRCm39)	S100G	probably benign	Het
Cmya5	G	T	13: 93,228,804 (GRCm39)	P2095T	probably damaging	Het
Dcaf10	T	C	4: 45,373,909 (GRCm39)	W445R	possibly damaging	Het
Espl1	T	A	15: 102,207,012 (GRCm39)	L159M	probably benign	Het
Fbxw8	T	C	5: 118,233,776 (GRCm39)	D285G	possibly damaging	Het
Fras1	C	T	5: 96,916,222 (GRCm39)	R3419W	probably damaging	Het
Galc	T	A	12: 98,197,672 (GRCm39)		probably null	Het
Ghdc	T	C	11: 100,660,543 (GRCm39)	E110G	probably damaging	Het
Gm4787	G	C	12: 81,424,604 (GRCm39)	T518S	probably benign	Het
H2-T24	T	C	17: 36,325,562 (GRCm39)	T309A	probably benign	Het
Helb	A	C	10: 119,937,391 (GRCm39)	F618V	probably damaging	Het
Hycc1	A	C	5: 24,170,220 (GRCm39)	S376R	possibly damaging	Het
Ifi211	A	G	1: 173,735,262 (GRCm39)	F56L	probably damaging	Het
Kntc1	T	C	5: 123,950,160 (GRCm39)	F1988L	probably damaging	Het
Mapk13	G	A	17: 28,997,465 (GRCm39)	S361N	probably benign	Het
Mapk3	A	G	7: 126,363,408 (GRCm39)	I146V	probably benign	Het
Megf6	G	A	4: 154,338,295 (GRCm39)		probably null	Het
Mug1	G	T	6: 121,838,092 (GRCm39)	V441F	probably benign	Het
Mymk	A	G	2: 26,952,226 (GRCm39)	S173P	probably benign	Het
Nox4	A	G	7: 87,023,616 (GRCm39)	T501A	possibly damaging	Het
Or13c7	C	A	4: 43,854,624 (GRCm39)	S105Y	possibly damaging	Het
Or1e16	AGCGGTCGTAGGC	AGC	11: 73,286,480 (GRCm39)		probably null	Het
Or52n2	T	C	7: 104,542,104 (GRCm39)	T244A	possibly damaging	Het
Prkcb	C	A	7: 121,888,678 (GRCm39)	H37Q	probably damaging	Het
Rabggtb	A	G	3: 153,615,024 (GRCm39)	F189L	probably damaging	Het
Setd1b	T	C	5: 123,281,471 (GRCm39)	I75T	unknown	Het
Slc10a5	T	A	3: 10,400,148 (GRCm39)	R171*	probably null	Het
Slc12a4	A	G	8: 106,680,484 (GRCm39)	F211L	probably damaging	Het
Slc1a5	T	A	7: 16,527,759 (GRCm39)	W352R	probably damaging	Het
Slc22a27	C	G	19: 7,843,303 (GRCm39)	A359P	probably damaging	Het
St8sia4	A	G	1: 95,555,460 (GRCm39)	M190T	probably damaging	Het
Tmeff2	A	G	1: 51,018,476 (GRCm39)	M153V	probably benign	Het
Trappc12	T	C	12: 28,796,696 (GRCm39)	T279A	probably damaging	Het
Ubn1	G	T	16: 4,895,818 (GRCm39)	A955S	probably benign	Het
Usp24	T	A	4: 106,239,500 (GRCm39)	H1147Q	possibly damaging	Het
Vrk1	T	A	12: 106,039,865 (GRCm39)		probably null	Het
Zfp109	A	G	7: 23,928,179 (GRCm39)	V418A	probably benign	Het

Other mutations in Zdhhc25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01999:Zdhhc25	APN	15	88,485,217 (GRCm39)	missense	probably damaging	0.98
IGL02448:Zdhhc25	APN	15	88,485,045 (GRCm39)	missense	probably damaging	1.00
IGL02576:Zdhhc25	APN	15	88,485,472 (GRCm39)	missense	probably benign	0.01
R0001:Zdhhc25	UTSW	15	88,485,112 (GRCm39)	missense	probably benign	0.00
R0394:Zdhhc25	UTSW	15	88,485,123 (GRCm39)	missense	probably damaging	0.96
R0622:Zdhhc25	UTSW	15	88,485,310 (GRCm39)	missense	probably damaging	0.98
R1116:Zdhhc25	UTSW	15	88,484,823 (GRCm39)	missense	probably benign	0.25
R1132:Zdhhc25	UTSW	15	88,484,926 (GRCm39)	missense	probably damaging	1.00
R1854:Zdhhc25	UTSW	15	88,484,689 (GRCm39)	missense	probably benign
R1994:Zdhhc25	UTSW	15	88,485,027 (GRCm39)	missense	probably benign	0.00
R2102:Zdhhc25	UTSW	15	88,484,962 (GRCm39)	missense	probably benign	0.10
R3749:Zdhhc25	UTSW	15	88,485,226 (GRCm39)	missense	probably benign	0.00
R5144:Zdhhc25	UTSW	15	88,485,259 (GRCm39)	missense	probably damaging	0.98
R9573:Zdhhc25	UTSW	15	88,485,307 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCATTGGTGAAGACAACCAG -3'
(R):5'- CTTTGCTCTGGACACCCATG -3'

Sequencing Primer
(F):5'- CAATGTACATTGGACTTACCTCGAC -3'
(R):5'- CTGGACACCCATGAATGACTTTAGG -3'

Posted On

2016-07-22