Mutagenetix > Incidental Mutation

Incidental Mutation 'R5766:Or13a25'

446278

Institutional Source

Beutler Lab

Gene Symbol

Or13a25

Ensembl Gene

ENSMUSG00000059136

Gene Name

olfactory receptor family 13 subfamily A member 25

Synonyms

GA_x6K02T2PBJ9-42813436-42814368, Olfr539, MOR253-4

MMRRC Submission

044424-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R5766 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

140247202-140248161 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 140247266 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 22 (V22E)

Ref Sequence

ENSEMBL: ENSMUSP00000077990 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078967] [ENSMUST00000210973] [ENSMUST00000218865]

AlphaFold

Q8VGL9

Predicted Effect

probably benign
Transcript: ENSMUST00000078967
AA Change: V22E

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000077990
Gene: ENSMUSG00000059136
AA Change: V22E

Domain	Start	End	E-Value	Type
Pfam:7tm_4	40	316	6.5e-50	PFAM
Pfam:7tm_1	50	299	4.9e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210499

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210973
AA Change: V15E

Predicted Effect

probably benign
Transcript: ENSMUST00000218865
AA Change: V15E

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219579

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219853

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl3	A	G	4: 144,182,439 (GRCm39)	V343A	probably damaging	Het
AI429214	TCCCTGATGAAC	TC	8: 37,461,383 (GRCm39)		probably null	Het
Arid2	A	G	15: 96,270,086 (GRCm39)	T1400A	probably benign	Het
Arrdc1	G	A	2: 24,816,417 (GRCm39)	T184I	probably damaging	Het
Aspm	T	C	1: 139,406,740 (GRCm39)	F1876L	probably damaging	Het
Calhm1	C	T	19: 47,132,142 (GRCm39)	V158I	probably benign	Het
Caly	T	C	7: 139,650,310 (GRCm39)	K211E	probably benign	Het
Cenpe	T	C	3: 134,954,174 (GRCm39)	L1677P	probably damaging	Het
Cttnbp2	A	G	6: 18,381,032 (GRCm39)	V1388A	possibly damaging	Het
Dgkd	C	T	1: 87,808,171 (GRCm39)	R80*	probably null	Het
Dnah3	A	T	7: 119,577,445 (GRCm39)	I2072N	probably damaging	Het
Dnah8	A	G	17: 30,909,235 (GRCm39)	I1020M	probably benign	Het
Drc3	T	C	11: 60,284,647 (GRCm39)	V484A	probably benign	Het
Efcab8	A	G	2: 153,622,912 (GRCm39)	D27G	possibly damaging	Het
Fat4	G	A	3: 38,943,617 (GRCm39)	G837R	probably damaging	Het
Fcgbpl1	G	A	7: 27,836,754 (GRCm39)	W224*	probably null	Het
Fgb	A	T	3: 82,953,483 (GRCm39)	C96S	probably damaging	Het
Flt4	G	C	11: 49,517,513 (GRCm39)	W278C	possibly damaging	Het
Fmnl2	T	C	2: 52,991,466 (GRCm39)	V327A	probably damaging	Het
Fras1	A	T	5: 96,879,548 (GRCm39)	Y2455F	possibly damaging	Het
Gm10549	C	A	18: 33,597,358 (GRCm39)		probably benign	Het
Gm13872	T	A	2: 102,568,254 (GRCm39)	I88N	probably damaging	Het
Hoxa6	A	G	6: 52,185,394 (GRCm39)	S71P	probably benign	Het
Hunk	T	A	16: 90,250,627 (GRCm39)	C148S	probably damaging	Het
Ighv1-18	A	C	12: 114,646,527 (GRCm39)	S26A	probably damaging	Het
Impg2	T	G	16: 56,080,183 (GRCm39)	D553E	possibly damaging	Het
Ints1	A	G	5: 139,757,900 (GRCm39)	I266T	probably benign	Het
Kpna3	G	T	14: 61,640,463 (GRCm39)	T33K	probably benign	Het
Lamb1	A	G	12: 31,349,930 (GRCm39)	D622G	probably damaging	Het
Lamp3	T	C	16: 19,520,067 (GRCm39)	T39A	probably damaging	Het
Mnt	C	T	11: 74,733,904 (GRCm39)		probably benign	Het
Msh4	A	G	3: 153,573,477 (GRCm39)	S726P	probably damaging	Het
Pah	A	C	10: 87,403,209 (GRCm39)	K195Q	probably damaging	Het
Pdc	T	A	1: 150,209,251 (GRCm39)	*245K	probably null	Het
Pdzd8	A	T	19: 59,288,972 (GRCm39)	H809Q	possibly damaging	Het
Plekhd1	A	T	12: 80,769,140 (GRCm39)	I467L	probably benign	Het
Ppp2ca	A	G	11: 52,004,014 (GRCm39)	D57G	probably damaging	Het
Ralgapa1	A	T	12: 55,867,551 (GRCm39)	M1K	probably null	Het
Rfx2	T	A	17: 57,110,587 (GRCm39)	D133V	probably benign	Het
Scfd2	A	T	5: 74,623,312 (GRCm39)	L407Q	probably damaging	Het
Scp2d1	T	A	2: 144,665,957 (GRCm39)	S99T	possibly damaging	Het
Sec63	A	T	10: 42,677,677 (GRCm39)	N261I	probably damaging	Het
Slc8a1	T	C	17: 81,956,390 (GRCm39)	Y216C	probably damaging	Het
Son	T	A	16: 91,461,875 (GRCm39)		probably benign	Het
Syce1	T	C	7: 140,357,894 (GRCm39)	E285G	probably damaging	Het
Taar7a	G	T	10: 23,869,260 (GRCm39)	S40R	probably benign	Het
Ube3a	A	T	7: 58,925,807 (GRCm39)	D216V	possibly damaging	Het
Vgll2	A	G	10: 51,903,659 (GRCm39)	D174G	probably damaging	Het
Vmn2r17	A	T	5: 109,575,139 (GRCm39)	T149S	possibly damaging	Het
Zbtb39	G	T	10: 127,578,557 (GRCm39)	C377F	probably damaging	Het
Zfp820	T	A	17: 22,038,983 (GRCm39)	N115I	probably damaging	Het
Zfp948	T	C	17: 21,805,078 (GRCm39)	S23P	probably benign	Het

Other mutations in Or13a25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00264:Or13a25	APN	7	140,247,854 (GRCm39)	missense	probably benign	0.01
IGL01610:Or13a25	APN	7	140,247,584 (GRCm39)	missense	probably damaging	1.00
IGL02959:Or13a25	APN	7	140,247,463 (GRCm39)	missense	probably damaging	1.00
IGL03406:Or13a25	APN	7	140,247,424 (GRCm39)	missense	probably damaging	1.00
R0671:Or13a25	UTSW	7	140,247,590 (GRCm39)	missense	probably damaging	1.00
R1771:Or13a25	UTSW	7	140,248,048 (GRCm39)	missense	probably benign
R1934:Or13a25	UTSW	7	140,247,951 (GRCm39)	nonsense	probably null
R1985:Or13a25	UTSW	7	140,247,734 (GRCm39)	missense	probably damaging	1.00
R2962:Or13a25	UTSW	7	140,247,862 (GRCm39)	missense	probably benign
R4239:Or13a25	UTSW	7	140,247,496 (GRCm39)	missense	probably benign	0.07
R4240:Or13a25	UTSW	7	140,247,496 (GRCm39)	missense	probably benign	0.07
R4360:Or13a25	UTSW	7	140,247,730 (GRCm39)	missense	probably damaging	0.98
R4841:Or13a25	UTSW	7	140,247,502 (GRCm39)	missense	probably damaging	1.00
R4842:Or13a25	UTSW	7	140,247,502 (GRCm39)	missense	probably damaging	1.00
R4851:Or13a25	UTSW	7	140,247,226 (GRCm39)	missense	probably benign
R5325:Or13a25	UTSW	7	140,247,705 (GRCm39)	missense	probably benign	0.33
R6363:Or13a25	UTSW	7	140,247,995 (GRCm39)	missense	possibly damaging	0.93
R6836:Or13a25	UTSW	7	140,248,093 (GRCm39)	missense	possibly damaging	0.86
R7777:Or13a25	UTSW	7	140,247,854 (GRCm39)	missense	probably benign	0.01
R7920:Or13a25	UTSW	7	140,247,814 (GRCm39)	missense	possibly damaging	0.92
R8134:Or13a25	UTSW	7	140,247,680 (GRCm39)	missense	possibly damaging	0.90
R8712:Or13a25	UTSW	7	140,248,052 (GRCm39)	missense	possibly damaging	0.89
R9095:Or13a25	UTSW	7	140,247,813 (GRCm39)	missense	probably damaging	1.00
R9158:Or13a25	UTSW	7	140,247,547 (GRCm39)	missense	possibly damaging	0.76
R9603:Or13a25	UTSW	7	140,247,794 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCTTACACGAAGGAAATACATTTG -3'
(R):5'- TGTTTTCCTCAGACACTAGGCC -3'

Sequencing Primer
(F):5'- CGAAGGAAATACATTTGTTCCGAGCC -3'
(R):5'- TACAGATAATGTCCATGGTGGCC -3'

Posted On

2016-11-21