Mutagenetix > Incidental Mutation

Incidental Mutation 'R7477:Or5w22'

579528

Institutional Source

Beutler Lab

Gene Symbol

Or5w22

Ensembl Gene

ENSMUSG00000061520

Gene Name

olfactory receptor family 5 subfamily W member 22

Synonyms

Olfr4-2, V5, GA_x6K02T2Q125-49033418-49034341, MOR177-5, Olfr153

MMRRC Submission

045551-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.108) question?

Stock #

R7477 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87362379-87363302 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 87362431 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 18 (N18S)

Ref Sequence

ENSEMBL: ENSMUSP00000149859 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000217113]

AlphaFold

Q7TR48

Predicted Effect

probably benign
Transcript: ENSMUST00000217113
AA Change: N18S

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

97% (66/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox3	G	T	5: 35,749,447 (GRCm39)	E191*	probably null	Het
Adam26a	T	A	8: 44,022,107 (GRCm39)	E461V	probably damaging	Het
Adamtsl1	G	A	4: 86,333,888 (GRCm39)	R1539Q	probably damaging	Het
Adipor2	A	T	6: 119,338,883 (GRCm39)	H123Q	probably benign	Het
Akap13	C	T	7: 75,398,995 (GRCm39)	S2691L	probably benign	Het
Aldh1l1	T	C	6: 90,575,369 (GRCm39)		probably null	Het
Ankrd11	A	T	8: 123,621,124 (GRCm39)	S909R	possibly damaging	Het
Bop1	T	C	15: 76,339,526 (GRCm39)	E273G	probably damaging	Het
Car1	G	A	3: 14,841,343 (GRCm39)	H97Y	probably damaging	Het
Casp14	T	C	10: 78,550,138 (GRCm39)	N170S	probably benign	Het
Ccdc158	A	T	5: 92,798,555 (GRCm39)	L382M	probably damaging	Het
Ccdc18	A	C	5: 108,368,716 (GRCm39)	Q1279H	probably damaging	Het
Cdh18	A	G	15: 23,410,811 (GRCm39)	N432S	probably benign	Het
Ctdp1	T	C	18: 80,483,929 (GRCm39)		probably null	Het
Dhx30	A	G	9: 109,916,208 (GRCm39)	I691T	probably damaging	Het
Dnah9	A	G	11: 65,883,557 (GRCm39)	I2562T	probably damaging	Het
Dsp	A	T	13: 38,356,839 (GRCm39)	I240F	probably damaging	Het
Ell	T	C	8: 71,037,868 (GRCm39)	S308P	probably benign	Het
Elmo1	A	G	13: 20,469,489 (GRCm39)	D26G		Het
Fam171a1	T	C	2: 3,226,676 (GRCm39)	V603A	probably benign	Het
Fam83e	G	A	7: 45,378,404 (GRCm39)	G476D	probably damaging	Het
Farp2	A	G	1: 93,508,750 (GRCm39)		probably null	Het
Fcer1a	C	T	1: 173,048,851 (GRCm39)		probably null	Het
Gpr179	G	T	11: 97,226,665 (GRCm39)	T1830K	possibly damaging	Het
Grin3a	G	A	4: 49,719,278 (GRCm39)	P823S	probably damaging	Het
Heatr4	T	A	12: 84,026,604 (GRCm39)	I218F	probably damaging	Het
Il36rn	T	A	2: 24,169,704 (GRCm39)	Y21*	probably null	Het
Jakmip1	A	G	5: 37,330,915 (GRCm39)	T532A	probably benign	Het
Klf11	A	G	12: 24,703,562 (GRCm39)	D16G	probably benign	Het
Lrp1	T	A	10: 127,404,789 (GRCm39)	I1971F	probably damaging	Het
Lrrc9	T	A	12: 72,550,301 (GRCm39)		probably null	Het
Lrrk2	C	T	15: 91,696,528 (GRCm39)	L2439F	probably damaging	Het
Lypd10	A	G	7: 24,413,673 (GRCm39)	T230A	probably benign	Het
Mapk14	G	A	17: 28,964,052 (GRCm39)	D313N	probably damaging	Het
Max	A	T	12: 76,999,960 (GRCm39)	S52T	probably benign	Het
Mrpl23	A	G	7: 142,091,018 (GRCm39)	R80G	possibly damaging	Het
Muc5ac	C	A	7: 141,370,019 (GRCm39)	N3186K	possibly damaging	Het
Mylk2	G	A	2: 152,762,261 (GRCm39)	V511I	probably damaging	Het
Mypn	T	C	10: 62,961,500 (GRCm39)	M1031V	possibly damaging	Het
Nbeal1	A	G	1: 60,300,743 (GRCm39)	T1488A	probably benign	Het
Ncbp1	A	T	4: 46,157,897 (GRCm39)	E378D	probably damaging	Het
Nedd9	A	T	13: 41,471,956 (GRCm39)	D174E	probably benign	Het
Nid2	T	A	14: 19,856,041 (GRCm39)	D1255E	probably benign	Het
Nlrc3	A	G	16: 3,782,675 (GRCm39)	C261R	probably damaging	Het
Pgam1	T	A	19: 41,905,255 (GRCm39)	H196Q	probably damaging	Het
Pja2	A	C	17: 64,616,640 (GRCm39)	V85G	possibly damaging	Het
Pkd2	G	A	5: 104,631,108 (GRCm39)	V511M	probably benign	Het
Ppp2cb	T	A	8: 34,105,502 (GRCm39)	S171T	probably benign	Het
Prkab2	T	C	3: 97,566,063 (GRCm39)	F45S	probably damaging	Het
Rabep2	A	T	7: 126,043,990 (GRCm39)		probably null	Het
Rnf41	T	A	10: 128,271,303 (GRCm39)	I71N	probably damaging	Het
Slc5a7	G	A	17: 54,588,787 (GRCm39)	P287S	probably damaging	Het
Smc6	A	G	12: 11,321,808 (GRCm39)	D25G	probably benign	Het
Sptb	A	T	12: 76,675,339 (GRCm39)	L225Q	probably damaging	Het
Tenm4	A	C	7: 96,495,015 (GRCm39)	I1148L	probably damaging	Het
Tnk2	C	A	16: 32,496,709 (GRCm39)		probably null	Het
Trank1	G	A	9: 111,194,025 (GRCm39)	S683N	probably benign	Het
Trp53bp1	A	C	2: 121,066,827 (GRCm39)	V633G	probably benign	Het
Ugt2a3	T	C	5: 87,484,479 (GRCm39)	K182E	possibly damaging	Het
Uncx	A	T	5: 139,533,017 (GRCm39)	T361S	probably benign	Het
Vmn2r11	A	T	5: 109,207,214 (GRCm39)	N35K	possibly damaging	Het
Vmn2r4	C	A	3: 64,305,850 (GRCm39)	R524L	probably benign	Het
Vmn2r5	C	T	3: 64,399,060 (GRCm39)	V640M	probably damaging	Het
Washc4	T	C	10: 83,410,307 (GRCm39)	Y632H	probably damaging	Het
Xkr6	T	C	14: 63,844,129 (GRCm39)	S51P	possibly damaging	Het
Zer1	C	A	2: 29,997,988 (GRCm39)	K408N	probably null	Het
Znhit2	A	G	19: 6,112,501 (GRCm39)		probably null	Het

Other mutations in Or5w22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01320:Or5w22	APN	2	87,362,629 (GRCm39)	missense	probably benign	0.01
IGL02102:Or5w22	APN	2	87,362,805 (GRCm39)	missense	probably benign
IGL02604:Or5w22	APN	2	87,362,949 (GRCm39)	missense	probably damaging	0.98
IGL02695:Or5w22	APN	2	87,362,461 (GRCm39)	missense	probably benign	0.00
IGL02961:Or5w22	APN	2	87,363,028 (GRCm39)	missense	probably damaging	0.98
PIT4696001:Or5w22	UTSW	2	87,363,124 (GRCm39)	missense	probably damaging	1.00
R0727:Or5w22	UTSW	2	87,363,245 (GRCm39)	nonsense	probably null
R1534:Or5w22	UTSW	2	87,363,016 (GRCm39)	missense	probably damaging	0.99
R1699:Or5w22	UTSW	2	87,362,427 (GRCm39)	missense	probably benign	0.07
R1885:Or5w22	UTSW	2	87,363,168 (GRCm39)	missense	probably damaging	0.99
R3705:Or5w22	UTSW	2	87,362,412 (GRCm39)	missense	probably benign	0.01
R5664:Or5w22	UTSW	2	87,363,178 (GRCm39)	missense	probably benign	0.35
R6492:Or5w22	UTSW	2	87,363,085 (GRCm39)	missense	possibly damaging	0.66
R6808:Or5w22	UTSW	2	87,363,285 (GRCm39)	missense	probably benign
R7432:Or5w22	UTSW	2	87,362,784 (GRCm39)	missense	probably damaging	1.00
R8014:Or5w22	UTSW	2	87,362,508 (GRCm39)	missense	probably benign	0.13
R8345:Or5w22	UTSW	2	87,362,691 (GRCm39)	missense	probably benign	0.01
R8887:Or5w22	UTSW	2	87,363,187 (GRCm39)	missense	possibly damaging	0.95
R8971:Or5w22	UTSW	2	87,362,580 (GRCm39)	missense	probably benign	0.19
R9311:Or5w22	UTSW	2	87,362,358 (GRCm39)	start gained	probably benign
R9690:Or5w22	UTSW	2	87,362,759 (GRCm39)	missense	probably benign	0.03
X0028:Or5w22	UTSW	2	87,362,383 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTGTCCATCCCAGATATTGGTC -3'
(R):5'- GGGACCAATTGCTGTGGAATAG -3'

Sequencing Primer
(F):5'- GTCCATCCCAGATATTGGTCATTTTG -3'
(R):5'- GCTGTGGAATAGCAAAGATCAC -3'

Posted On

2019-10-07