Mutagenetix > Incidental Mutation

Incidental Mutation 'R0855:Vmn2r109'

82703

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

039034-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R0855 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

20760779-20785018 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 20761670 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 562 (Y562*)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably null
Transcript: ENSMUST00000167093
AA Change: Y562*

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: Y562*

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 94.9%

Validation Efficiency

97% (35/36)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700046A07Rik	A	G	18: 62,886,414 (GRCm39)		noncoding transcript	Het
Ak3	T	C	19: 29,000,345 (GRCm39)	K189E	probably benign	Het
Anks3	A	T	16: 4,773,811 (GRCm39)		probably benign	Het
Ash1l	C	A	3: 88,961,761 (GRCm39)	H2378N	possibly damaging	Het
Baz1a	A	G	12: 54,947,348 (GRCm39)		probably benign	Het
Bicra	A	G	7: 15,705,929 (GRCm39)	F1504S	probably damaging	Het
Blzf1	T	C	1: 164,119,950 (GRCm39)	T353A	possibly damaging	Het
Btn1a1	C	A	13: 23,648,489 (GRCm39)	V115F	probably damaging	Het
Cd38	T	A	5: 44,060,927 (GRCm39)		probably null	Het
Cep250	T	C	2: 155,806,031 (GRCm39)	C109R	probably damaging	Het
Cnksr1	C	T	4: 133,960,377 (GRCm39)		probably benign	Het
Dmxl2	T	A	9: 54,273,724 (GRCm39)	N3048I	probably benign	Het
Impdh1	T	A	6: 29,206,971 (GRCm39)	H116L	probably damaging	Het
Kank4	C	A	4: 98,659,681 (GRCm39)	W799L	probably damaging	Het
Kcnk7	C	T	19: 5,756,103 (GRCm39)	H110Y	probably benign	Het
Mak	T	C	13: 41,223,640 (GRCm39)	E25G	probably damaging	Het
Mrpl54	G	A	10: 81,102,759 (GRCm39)		probably benign	Het
Myh10	A	C	11: 68,702,627 (GRCm39)	D1767A	possibly damaging	Het
Naip2	T	C	13: 100,298,362 (GRCm39)	E558G	probably benign	Het
Naip2	C	T	13: 100,298,368 (GRCm39)	G556D	probably benign	Het
Ndufaf6	A	T	4: 11,051,169 (GRCm39)	H310Q	probably damaging	Het
Osbpl6	G	T	2: 76,415,477 (GRCm39)	G467V	probably damaging	Het
Osbpl6	A	G	2: 76,422,183 (GRCm39)	E673G	probably damaging	Het
Picalm	T	A	7: 89,840,356 (GRCm39)	D458E	possibly damaging	Het
Ppp2ca	G	A	11: 52,012,752 (GRCm39)	R294H	probably benign	Het
Prdm14	T	A	1: 13,195,761 (GRCm39)	N100I	probably benign	Het
Rbbp6	A	G	7: 122,591,471 (GRCm39)	T510A	probably benign	Het
Sars1	C	A	3: 108,334,248 (GRCm39)	E503D	probably benign	Het
Smtn	T	C	11: 3,471,880 (GRCm39)	D853G	probably damaging	Het
Tbx20	A	G	9: 24,636,908 (GRCm39)	M393T	probably benign	Het
Thada	T	C	17: 84,744,083 (GRCm39)	T742A	probably damaging	Het
Tmem63a	T	C	1: 180,788,625 (GRCm39)	S321P	possibly damaging	Het
Trim24	T	A	6: 37,892,137 (GRCm39)	C223*	probably null	Het
Usp48	T	C	4: 137,335,465 (GRCm39)	F213L	probably damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20,770,419 (GRCm39)	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20,761,383 (GRCm39)	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20,761,671 (GRCm39)	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20,774,654 (GRCm39)	missense	probably benign
IGL01864:Vmn2r109	APN	17	20,761,396 (GRCm39)	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20,761,342 (GRCm39)	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20,774,603 (GRCm39)	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20,774,422 (GRCm39)	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20,761,150 (GRCm39)	missense	probably benign
IGL02490:Vmn2r109	APN	17	20,761,246 (GRCm39)	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20,760,963 (GRCm39)	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20,774,518 (GRCm39)	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20,774,062 (GRCm39)	missense	probably benign
IGL02745:Vmn2r109	APN	17	20,761,512 (GRCm39)	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20,774,839 (GRCm39)	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20,773,148 (GRCm39)	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20,760,937 (GRCm39)	missense	probably damaging	0.99
R0882:Vmn2r109	UTSW	17	20,774,842 (GRCm39)	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20,775,503 (GRCm39)	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20,761,002 (GRCm39)	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20,774,072 (GRCm39)	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20,774,185 (GRCm39)	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20,761,448 (GRCm39)	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20,784,974 (GRCm39)	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20,774,798 (GRCm39)	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20,761,248 (GRCm39)	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20,774,704 (GRCm39)	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20,774,074 (GRCm39)	missense	probably benign
R4428:Vmn2r109	UTSW	17	20,773,286 (GRCm39)	missense	probably benign
R4584:Vmn2r109	UTSW	17	20,774,820 (GRCm39)	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20,761,656 (GRCm39)	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20,761,605 (GRCm39)	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20,774,153 (GRCm39)	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20,761,494 (GRCm39)	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20,770,348 (GRCm39)	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20,775,451 (GRCm39)	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20,774,603 (GRCm39)	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20,761,189 (GRCm39)	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20,760,933 (GRCm39)	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20,760,781 (GRCm39)	makesense	probably null
R5702:Vmn2r109	UTSW	17	20,774,407 (GRCm39)	missense	probably benign	0.42
R5706:Vmn2r109	UTSW	17	20,774,567 (GRCm39)	missense	probably benign	0.16
R5714:Vmn2r109	UTSW	17	20,773,121 (GRCm39)	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20,761,318 (GRCm39)	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20,760,981 (GRCm39)	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20,761,440 (GRCm39)	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20,784,796 (GRCm39)	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20,774,785 (GRCm39)	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20,760,932 (GRCm39)	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20,760,973 (GRCm39)	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20,785,006 (GRCm39)	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20,761,225 (GRCm39)	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20,760,945 (GRCm39)	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20,761,700 (GRCm39)	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20,761,043 (GRCm39)	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20,761,536 (GRCm39)	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20,774,665 (GRCm39)	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20,760,942 (GRCm39)	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20,773,117 (GRCm39)	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20,761,436 (GRCm39)	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20,760,782 (GRCm39)	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20,774,729 (GRCm39)	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20,774,531 (GRCm39)	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20,775,332 (GRCm39)	missense
Z1176:Vmn2r109	UTSW	17	20,773,256 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TGTGATGAGCAAAGTGTAACTGAGACC -3'
(R):5'- TCAGGCCCAATGTCATTATCTGCAAG -3'

Sequencing Primer
(F):5'- GTGTAACTGAGACCTCTATTATTGGC -3'
(R):5'- GCTAGTTCAAGTCACTATAAAGAAGG -3'

Posted On

2013-11-08