Incidental Mutation 'R2159:Ptp4a3'
ID 235076
Institutional Source Beutler Lab
Gene Symbol Ptp4a3
Ensembl Gene ENSMUSG00000059895
Gene Name protein tyrosine phosphatase 4a3
Synonyms Prl-3
MMRRC Submission 040162-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.121) question?
Stock # R2159 (G1)
Quality Score 225
Status Not validated
Chromosome 15
Chromosomal Location 73594991-73629075 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 73623865 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 32 (T32A)
Ref Sequence ENSEMBL: ENSMUSP00000155773 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053232] [ENSMUST00000163582] [ENSMUST00000165541] [ENSMUST00000167582] [ENSMUST00000230044] [ENSMUST00000230177] [ENSMUST00000230307] [ENSMUST00000231209]
AlphaFold Q9D658
Predicted Effect probably benign
Transcript: ENSMUST00000053232
AA Change: T32A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000060956
Gene: ENSMUSG00000059895
AA Change: T32A

DomainStartEndE-ValueType
Pfam:DSPc 26 155 7.1e-10 PFAM
Pfam:Y_phosphatase 27 153 3.9e-11 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000163582
AA Change: T32A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000131281
Gene: ENSMUSG00000059895
AA Change: T32A

DomainStartEndE-ValueType
Pfam:DSPc 26 155 7.1e-10 PFAM
Pfam:Y_phosphatase 27 153 3.9e-11 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000165541
AA Change: T32A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000132097
Gene: ENSMUSG00000059895
AA Change: T32A

DomainStartEndE-ValueType
Pfam:Y_phosphatase 13 152 5.1e-9 PFAM
Pfam:DSPc 34 154 4.8e-9 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000167582
SMART Domains Protein: ENSMUSP00000131036
Gene: ENSMUSG00000059895

DomainStartEndE-ValueType
Pfam:Y_phosphatase 5 134 7.8e-12 PFAM
Pfam:DSPc 5 136 1.8e-10 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000229339
Predicted Effect probably benign
Transcript: ENSMUST00000230044
AA Change: T32A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
Predicted Effect probably benign
Transcript: ENSMUST00000230177
AA Change: T32A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
Predicted Effect probably benign
Transcript: ENSMUST00000230307
AA Change: T32A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
Predicted Effect probably benign
Transcript: ENSMUST00000231209
AA Change: T32A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230864
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.4%
  • 20x: 95.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the protein-tyrosine phosphatase family. Protein tyrosine phosphatases are cell signaling molecules that play regulatory roles in a variety of cellular processes. Studies of this class of protein tyrosine phosphatase in mice demonstrates that they are prenylated in vivo, suggesting their association with cell plasma membrane. The encoded protein may enhance cell proliferation, and overexpression of this gene has been implicated in tumor metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit partial preweaning lethality and decreased incidence of AOM-DDS induced tumors. Mice homozygous for a different targeted allele exhibit decreased circulating glucose levels in an intraperitoneal glucose tolerance test. [provided by MGI curators]
Allele List at MGI

All alleles(11) : Targeted(2) Gene trapped(9)
Other mutations in this stock
Total: 60 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam21 G A 12: 81,607,241 (GRCm39) P174S probably benign Het
Aida C T 1: 183,103,234 (GRCm39) P185S probably benign Het
Alg11 T G 8: 22,555,861 (GRCm39) I374R probably benign Het
Ano9 T C 7: 140,688,030 (GRCm39) I229V probably benign Het
Apob A T 12: 8,060,081 (GRCm39) L2821F probably benign Het
Atp10b C T 11: 43,042,680 (GRCm39) T80I possibly damaging Het
BC024139 T C 15: 76,005,688 (GRCm39) H478R probably damaging Het
Btbd1 A T 7: 81,450,804 (GRCm39) D325E possibly damaging Het
Camk2a G A 18: 61,090,257 (GRCm39) C199Y probably damaging Het
Casp3 A G 8: 47,087,323 (GRCm39) D90G probably damaging Het
Ccnt2 A G 1: 127,702,891 (GRCm39) H71R probably benign Het
Cdk2ap1 G A 5: 124,486,667 (GRCm39) R65* probably null Het
Cebpb G T 2: 167,531,173 (GRCm39) A78S probably benign Het
Col5a3 A G 9: 20,682,606 (GRCm39) F1613L unknown Het
Cpd T C 11: 76,688,467 (GRCm39) D935G probably damaging Het
Csmd3 CCTTTGCGCTT CCTT 15: 47,604,632 (GRCm39) probably null Het
Cwc27 A G 13: 104,940,837 (GRCm39) I174T probably damaging Het
Cyp21a1 C A 17: 35,021,378 (GRCm39) R331L probably benign Het
Dnah5 A G 15: 28,252,691 (GRCm39) T795A probably benign Het
Eif2ak2 A G 17: 79,181,447 (GRCm39) V100A possibly damaging Het
Foxa3 A G 7: 18,748,109 (GRCm39) V339A probably benign Het
Gp2 T C 7: 119,051,507 (GRCm39) D236G probably benign Het
Gprc6a CAAA CA 10: 51,491,776 (GRCm39) probably null Het
Gzmd T A 14: 56,368,153 (GRCm39) H102L probably damaging Het
Klk1b1 A T 7: 43,619,857 (GRCm39) I139F probably damaging Het
Lcmt2 G A 2: 120,969,766 (GRCm39) P439L probably damaging Het
Loxl4 C T 19: 42,588,446 (GRCm39) A570T probably damaging Het
Mga T A 2: 119,750,124 (GRCm39) H674Q probably damaging Het
Mybpc3 A T 2: 90,955,715 (GRCm39) K583M probably damaging Het
Ncoa6 G T 2: 155,249,633 (GRCm39) P1224T probably damaging Het
Nostrin A G 2: 69,011,266 (GRCm39) probably null Het
Or13e8 T C 4: 43,697,110 (GRCm39) H21R probably benign Het
Or4c3 A T 2: 89,851,882 (GRCm39) V176E probably damaging Het
Oxsr1 A C 9: 119,133,880 (GRCm39) D58E possibly damaging Het
Parm1 A G 5: 91,760,923 (GRCm39) Y265C probably damaging Het
Phf10 C T 17: 15,172,926 (GRCm39) E304K probably damaging Het
Prmt5 T C 14: 54,752,795 (GRCm39) T139A probably benign Het
Ptgir A G 7: 16,640,794 (GRCm39) M29V possibly damaging Het
Pwwp2b A T 7: 138,834,844 (GRCm39) D95V possibly damaging Het
Rad51ap2 T C 12: 11,507,752 (GRCm39) L558S possibly damaging Het
Rapgef4 A G 2: 72,005,225 (GRCm39) D80G probably damaging Het
Sec24a T G 11: 51,603,177 (GRCm39) H757P probably damaging Het
Sema4d T C 13: 51,874,571 (GRCm39) N129D probably damaging Het
Serpini1 A G 3: 75,531,251 (GRCm39) T323A probably benign Het
Setd1a G A 7: 127,384,661 (GRCm39) R504H possibly damaging Het
Sftpb T C 6: 72,286,770 (GRCm39) C226R probably damaging Het
Sp8 T C 12: 118,812,441 (GRCm39) S99P possibly damaging Het
Srgap3 A G 6: 112,748,339 (GRCm39) F438L probably damaging Het
Stim2 T C 5: 54,267,156 (GRCm39) Y365H probably damaging Het
Stk36 A G 1: 74,673,896 (GRCm39) Q1263R probably benign Het
Tectb C G 19: 55,169,431 (GRCm39) probably benign Het
Troap G T 15: 98,975,467 (GRCm39) A184S probably damaging Het
Ttn G A 2: 76,729,671 (GRCm39) probably benign Het
Vmn2r108 C A 17: 20,689,363 (GRCm39) A531S probably benign Het
Vmn2r12 T A 5: 109,239,340 (GRCm39) I408F probably benign Het
Vmn2r8 T A 5: 108,950,169 (GRCm39) E226V probably benign Het
Vmo1 A G 11: 70,404,608 (GRCm39) F131S probably benign Het
Vwf T C 6: 125,603,304 (GRCm39) F885L probably damaging Het
Wdpcp A T 11: 21,807,476 (GRCm39) M618L probably benign Het
Wdr90 T C 17: 26,070,715 (GRCm39) E1072G probably benign Het
Other mutations in Ptp4a3
AlleleSourceChrCoordTypePredicted EffectPPH Score
P0005:Ptp4a3 UTSW 15 73,627,160 (GRCm39) missense possibly damaging 0.95
R2033:Ptp4a3 UTSW 15 73,625,618 (GRCm39) missense probably damaging 1.00
R5976:Ptp4a3 UTSW 15 73,627,885 (GRCm39) missense possibly damaging 0.89
R6874:Ptp4a3 UTSW 15 73,595,259 (GRCm39) unclassified probably benign
R7894:Ptp4a3 UTSW 15 73,628,756 (GRCm39) missense probably benign 0.02
R8168:Ptp4a3 UTSW 15 73,628,695 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCTTTGTGGACAAGCACGGG -3'
(R):5'- AATCTGCCTGTCTCTGAGCC -3'

Sequencing Primer
(F):5'- ACGGGGATCTCACTGGACTTG -3'
(R):5'- CTGTCTCTGCAGAGGCTAAG -3'
Posted On 2014-10-01