Mutagenetix > Incidental Mutation

Incidental Mutation 'R0490:Tas2r116'

43438

Institutional Source

Beutler Lab

Gene Symbol

Tas2r116

Ensembl Gene

ENSMUSG00000030194

Gene Name

taste receptor, type 2, member 116

Synonyms

mGR16, TRB1, Tas2r7, Tas2r16, T2R16, mt2r56, Tas2r14, TRB4

MMRRC Submission

038688-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0490 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132832401-132833318 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 132832984 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 195 (V195A)

Ref Sequence

ENSEMBL: ENSMUSP00000032315 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032315]

AlphaFold

Q7M713

Predicted Effect

probably benign
Transcript: ENSMUST00000032315
AA Change: V195A

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000032315
Gene: ENSMUSG00000030194
AA Change: V195A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	300	2.8e-89	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203358

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 96.2%
20x: 92.2%

Validation Efficiency

100% (60/60)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9330159F19Rik	T	A	10: 29,103,338 (GRCm39)	Y640N	probably damaging	Het
Adamts9	T	A	6: 92,849,847 (GRCm39)	Q402L	probably benign	Het
Als2cl	A	G	9: 110,724,414 (GRCm39)	T750A	probably benign	Het
Ank1	C	A	8: 23,597,890 (GRCm39)		probably benign	Het
Ap4e1	T	A	2: 126,888,106 (GRCm39)	N404K	probably damaging	Het
Atf7ip	G	T	6: 136,586,190 (GRCm39)		probably benign	Het
Bean1	A	T	8: 104,941,660 (GRCm39)	T169S	possibly damaging	Het
Bod1l	G	T	5: 41,979,235 (GRCm39)	T693N	probably damaging	Het
Ccdc81	A	T	7: 89,536,970 (GRCm39)	V226D	probably benign	Het
Cd48	A	G	1: 171,532,445 (GRCm39)	*241W	probably null	Het
Cdon	C	A	9: 35,363,978 (GRCm39)	S32Y	probably damaging	Het
Cers3	A	G	7: 66,423,438 (GRCm39)	S128G	possibly damaging	Het
Cntn6	T	C	6: 104,810,879 (GRCm39)	V641A	possibly damaging	Het
Col6a4	G	A	9: 105,890,969 (GRCm39)	T1775I	probably damaging	Het
Dnah8	T	C	17: 30,919,393 (GRCm39)	V1122A	probably benign	Het
Dst	G	T	1: 34,346,449 (GRCm39)	G5102*	probably null	Het
Dvl3	C	T	16: 20,346,173 (GRCm39)		probably benign	Het
Epha5	G	T	5: 84,255,833 (GRCm39)		probably benign	Het
Fscb	G	A	12: 64,519,661 (GRCm39)	P602S	unknown	Het
Fxn	A	G	19: 24,254,543 (GRCm39)		probably null	Het
Gipc2	A	G	3: 151,808,291 (GRCm39)	L254P	possibly damaging	Het
Gm973	C	T	1: 59,597,393 (GRCm39)		probably benign	Het
Gng8	A	G	7: 16,628,908 (GRCm39)	T14A	probably benign	Het
Gsdmc3	C	T	15: 63,732,099 (GRCm39)	G309D	possibly damaging	Het
Gsr	T	A	8: 34,161,540 (GRCm39)		probably benign	Het
Gtdc1	A	T	2: 44,525,052 (GRCm39)	D152E	probably benign	Het
Herc1	A	G	9: 66,392,281 (GRCm39)	D4063G	probably damaging	Het
Hsdl2	C	T	4: 59,612,814 (GRCm39)		probably benign	Het
Iqgap3	T	C	3: 88,021,363 (GRCm39)		probably benign	Het
Kcnj10	A	G	1: 172,197,019 (GRCm39)	T178A	probably damaging	Het
Lama5	T	A	2: 179,821,962 (GRCm39)	I2958F	possibly damaging	Het
Lnx1	T	A	5: 74,781,008 (GRCm39)		probably null	Het
Lpl	A	G	8: 69,349,343 (GRCm39)	R290G	probably damaging	Het
Mamdc4	C	T	2: 25,453,593 (GRCm39)	R1196K	probably benign	Het
Mogat2	T	C	7: 98,872,351 (GRCm39)	S167G	probably benign	Het
Nek8	T	A	11: 78,058,555 (GRCm39)	I582F	probably benign	Het
Notch4	T	A	17: 34,801,864 (GRCm39)	D1237E	probably damaging	Het
Or2y8	A	G	11: 52,035,493 (GRCm39)	I288T	probably damaging	Het
Or5b104	A	G	19: 13,072,176 (GRCm39)	Y279H	probably damaging	Het
Or5b119	G	A	19: 13,456,857 (GRCm39)	A235V	probably damaging	Het
Or9i14	A	G	19: 13,792,219 (GRCm39)	L245P	probably damaging	Het
Pcdhb8	T	C	18: 37,489,833 (GRCm39)	S504P	probably damaging	Het
Pigs	T	C	11: 78,226,451 (GRCm39)	S223P	probably damaging	Het
Prkch	A	G	12: 73,806,450 (GRCm39)	I566V	probably damaging	Het
Ptpn22	T	C	3: 103,793,495 (GRCm39)	S549P	probably damaging	Het
Rita1	A	T	5: 120,749,630 (GRCm39)	F28I	probably damaging	Het
Rpgrip1l	A	T	8: 92,026,473 (GRCm39)		probably benign	Het
Slc1a1	A	G	19: 28,874,931 (GRCm39)	K170E	probably benign	Het
Spag17	C	T	3: 99,889,727 (GRCm39)	R199W	probably damaging	Het
Trav7d-3	C	A	14: 52,982,007 (GRCm39)		probably benign	Het
Trim15	T	C	17: 37,177,247 (GRCm39)	K138E	probably benign	Het
Ttn	T	A	2: 76,539,174 (GRCm39)	H34604L	probably benign	Het
Ttn	C	T	2: 76,577,876 (GRCm39)	R16012K	probably damaging	Het
Zfp948	T	C	17: 21,808,296 (GRCm39)	V496A	probably benign	Het
Zfy2	A	T	Y: 2,106,620 (GRCm39)	S671R	possibly damaging	Het
Zswim1	T	A	2: 164,667,203 (GRCm39)	Y152N	possibly damaging	Het

Other mutations in Tas2r116

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01305:Tas2r116	APN	6	132,832,406 (GRCm39)	missense	probably benign	0.02
IGL01656:Tas2r116	APN	6	132,832,396 (GRCm39)	unclassified	probably benign
IGL01970:Tas2r116	APN	6	132,832,632 (GRCm39)	missense	probably benign	0.07
ANU22:Tas2r116	UTSW	6	132,832,406 (GRCm39)	missense	probably benign	0.02
R2422:Tas2r116	UTSW	6	132,832,557 (GRCm39)	missense	possibly damaging	0.88
R4013:Tas2r116	UTSW	6	132,833,230 (GRCm39)	missense	probably damaging	1.00
R4516:Tas2r116	UTSW	6	132,833,113 (GRCm39)	missense	probably damaging	1.00
R4745:Tas2r116	UTSW	6	132,832,668 (GRCm39)	missense	probably benign	0.14
R4842:Tas2r116	UTSW	6	132,832,660 (GRCm39)	missense	probably benign	0.00
R7723:Tas2r116	UTSW	6	132,832,867 (GRCm39)	missense	probably benign	0.25
R8439:Tas2r116	UTSW	6	132,832,540 (GRCm39)	missense	probably damaging	0.99
R8551:Tas2r116	UTSW	6	132,832,993 (GRCm39)	missense	probably benign	0.01
R9009:Tas2r116	UTSW	6	132,832,963 (GRCm39)	missense	probably damaging	1.00
R9573:Tas2r116	UTSW	6	132,833,140 (GRCm39)	missense	probably benign	0.04
Z1088:Tas2r116	UTSW	6	132,832,911 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCTTTACCCAGGACAATGGATGAC -3'
(R):5'- AAAAGCTGTTCTTGCAACCTGTGTG -3'

Sequencing Primer
(F):5'- GGTTTGCTACAAGTCTCAGC -3'
(R):5'- TGCAACCTGTGTGATCAAAAGC -3'

Posted On

2013-05-23