Mutagenetix > Incidental Mutation

Incidental Mutation 'R6575:Zfp938'

523406

Institutional Source

Beutler Lab

Gene Symbol

Zfp938

Ensembl Gene

ENSMUSG00000062931

Gene Name

zinc finger protein 938

Synonyms

B230315N10Rik

MMRRC Submission

044699-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R6575 (G1)

Quality Score

134.008

Status

Not validated

Chromosome

Chromosomal Location

82060684-82077114 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 82061160 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Stop codon at position 487 (G487*)

Ref Sequence

ENSEMBL: ENSMUSP00000047110 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041264] [ENSMUST00000156218]

AlphaFold

E9Q9G3

Predicted Effect

probably null
Transcript: ENSMUST00000041264
AA Change: G487*

SMART Domains

Protein: ENSMUSP00000047110
Gene: ENSMUSG00000062931
AA Change: G487*

Domain	Start	End	E-Value	Type
KRAB	4	64	5.28e-14	SMART
ZnF_C2H2	161	188	2.82e1	SMART
ZnF_C2H2	267	289	1.23e0	SMART
ZnF_C2H2	295	317	2.91e-2	SMART
ZnF_C2H2	323	345	2.4e-3	SMART
ZnF_C2H2	351	373	7.26e-3	SMART
ZnF_C2H2	379	401	4.65e-1	SMART
ZnF_C2H2	407	429	1.47e-3	SMART
ZnF_C2H2	435	457	5.59e-4	SMART
ZnF_C2H2	463	485	1.82e-3	SMART
ZnF_C2H2	491	513	3.63e-3	SMART
ZnF_C2H2	519	541	7.67e-2	SMART
ZnF_C2H2	547	569	2.4e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000156218

SMART Domains

Protein: ENSMUSP00000121613
Gene: ENSMUSG00000062931

Domain	Start	End	E-Value	Type
KRAB	4	64	5.28e-14	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	T	C	5: 113,330,683 (GRCm39)	Y1177C	probably damaging	Het
Acnat1	A	G	4: 49,450,785 (GRCm39)	Y109H	possibly damaging	Het
Adgrf3	T	A	5: 30,401,522 (GRCm39)	K835N	possibly damaging	Het
Afg1l	T	C	10: 42,194,712 (GRCm39)	D360G	probably damaging	Het
Ap3s1	C	T	18: 46,887,448 (GRCm39)	T27M	probably benign	Het
Atp8b4	A	G	2: 126,256,284 (GRCm39)	L225P	probably damaging	Het
Bicra	G	A	7: 15,713,056 (GRCm39)	T997I	probably benign	Het
Bod1l	T	C	5: 41,995,411 (GRCm39)	R112G	probably damaging	Het
Cabin1	T	C	10: 75,561,535 (GRCm39)	T989A	possibly damaging	Het
Cog1	C	A	11: 113,546,887 (GRCm39)	Q494K	probably benign	Het
Cyb5rl	T	C	4: 106,942,550 (GRCm39)	Y160H	probably benign	Het
Dnajb8	A	G	6: 88,200,057 (GRCm39)	N198D	probably damaging	Het
Eif1ad11	T	C	12: 87,994,117 (GRCm39)	I115T	probably damaging	Het
Eif3l	C	A	15: 78,970,778 (GRCm39)	Q351K	possibly damaging	Het
Esr1	T	C	10: 4,916,301 (GRCm39)		probably benign	Het
Fam3c	G	T	6: 22,329,607 (GRCm39)	A40D	probably damaging	Het
Fcrla	A	G	1: 170,749,797 (GRCm39)	S87P	probably damaging	Het
Glra1	A	G	11: 55,411,822 (GRCm39)	Y246H	probably damaging	Het
Gm45871	T	A	18: 90,609,844 (GRCm39)	C361S	probably damaging	Het
Greb1l	A	G	18: 10,547,347 (GRCm39)	N1522D	possibly damaging	Het
Hcn4	T	C	9: 58,731,435 (GRCm39)	M214T	unknown	Het
Heatr5b	A	T	17: 79,070,418 (GRCm39)	V1665E	probably damaging	Het
Hemgn	C	A	4: 46,395,990 (GRCm39)	M415I	possibly damaging	Het
Hibadh	G	A	6: 52,524,013 (GRCm39)	T295I	probably damaging	Het
Iars1	T	C	13: 49,878,745 (GRCm39)	L947P	probably damaging	Het
Icam2	G	A	11: 106,269,585 (GRCm39)	T178I	probably damaging	Het
Lbr	A	G	1: 181,663,763 (GRCm39)	S86P	probably damaging	Het
Lipe	G	A	7: 25,082,749 (GRCm39)	T801I	probably benign	Het
Lrp6	T	C	6: 134,518,934 (GRCm39)	T44A	possibly damaging	Het
Malrd1	A	G	2: 15,847,439 (GRCm39)	H1193R	probably benign	Het
Mroh2a	GCCC	GC	1: 88,159,979 (GRCm39)		probably null	Het
Myh10	A	G	11: 68,699,676 (GRCm39)	I1671V	probably benign	Het
Or2c1	A	G	16: 3,656,894 (GRCm39)	D19G	probably benign	Het
Osbpl9	T	C	4: 108,930,129 (GRCm39)	D334G	possibly damaging	Het
Pdcd11	A	G	19: 47,098,117 (GRCm39)	D801G	probably damaging	Het
Pfdn4	C	A	2: 170,358,556 (GRCm39)	D16E	probably benign	Het
Pmp22	C	T	11: 63,049,099 (GRCm39)	A114V	probably damaging	Het
Rrp9	C	T	9: 106,360,778 (GRCm39)	T253I	probably damaging	Het
Speer1m	A	T	5: 11,970,612 (GRCm39)	K62*	probably null	Het
Tnrc6a	A	G	7: 122,769,133 (GRCm39)	T308A	probably damaging	Het
Trgv4	T	A	13: 19,369,250 (GRCm39)	C34S	probably benign	Het
Trhr	A	G	15: 44,092,602 (GRCm39)	M280V	possibly damaging	Het
Trp53bp1	G	A	2: 121,059,084 (GRCm39)	H926Y	probably damaging	Het
Trpv5	A	T	6: 41,652,903 (GRCm39)	I90K	probably benign	Het
Ttn	T	C	2: 76,719,259 (GRCm39)		probably benign	Het
Vav1	A	C	17: 57,612,280 (GRCm39)	R513S	probably damaging	Het
Vmn2r100	A	T	17: 19,741,671 (GRCm39)	T128S	probably benign	Het
Zfp687	T	C	3: 94,915,700 (GRCm39)	Y1024C	probably damaging	Het
Znrf2	A	G	6: 54,855,430 (GRCm39)	Y73C	probably damaging	Het

Other mutations in Zfp938

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00659:Zfp938	APN	10	82,063,355 (GRCm39)	utr 3 prime	probably benign
IGL00743:Zfp938	APN	10	82,062,317 (GRCm39)	missense	probably benign
IGL01764:Zfp938	APN	10	82,063,624 (GRCm39)	splice site	probably benign
IGL01814:Zfp938	APN	10	82,062,052 (GRCm39)	missense	probably benign
IGL02244:Zfp938	APN	10	82,061,906 (GRCm39)	missense	possibly damaging	0.86
IGL02865:Zfp938	APN	10	82,062,026 (GRCm39)	missense	probably benign	0.33
R0372:Zfp938	UTSW	10	82,063,662 (GRCm39)	missense	probably damaging	1.00
R0666:Zfp938	UTSW	10	82,061,606 (GRCm39)	missense	probably damaging	1.00
R0964:Zfp938	UTSW	10	82,061,253 (GRCm39)	missense	probably benign	0.00
R1453:Zfp938	UTSW	10	82,063,632 (GRCm39)	critical splice donor site	probably null
R1672:Zfp938	UTSW	10	82,060,982 (GRCm39)	missense	probably benign
R1929:Zfp938	UTSW	10	82,061,381 (GRCm39)	missense	probably damaging	1.00
R1959:Zfp938	UTSW	10	82,061,465 (GRCm39)	missense	probably damaging	1.00
R2127:Zfp938	UTSW	10	82,061,876 (GRCm39)	missense	probably benign
R2271:Zfp938	UTSW	10	82,061,381 (GRCm39)	missense	probably damaging	1.00
R2900:Zfp938	UTSW	10	82,061,340 (GRCm39)	missense	possibly damaging	0.92
R4502:Zfp938	UTSW	10	82,062,105 (GRCm39)	missense	possibly damaging	0.73
R4503:Zfp938	UTSW	10	82,062,105 (GRCm39)	missense	possibly damaging	0.73
R4886:Zfp938	UTSW	10	82,061,957 (GRCm39)	missense	probably benign	0.33
R4934:Zfp938	UTSW	10	82,062,012 (GRCm39)	missense	possibly damaging	0.86
R5174:Zfp938	UTSW	10	82,061,838 (GRCm39)	missense	possibly damaging	0.53
R5410:Zfp938	UTSW	10	82,061,092 (GRCm39)	missense	possibly damaging	0.89
R6284:Zfp938	UTSW	10	82,063,400 (GRCm39)	missense	possibly damaging	0.73
R6491:Zfp938	UTSW	10	82,063,363 (GRCm39)	makesense	probably null
R6649:Zfp938	UTSW	10	82,061,232 (GRCm39)	missense	probably damaging	0.99
R7992:Zfp938	UTSW	10	82,061,777 (GRCm39)	missense	possibly damaging	0.53
R8211:Zfp938	UTSW	10	82,062,419 (GRCm39)	missense	possibly damaging	0.53
R8313:Zfp938	UTSW	10	82,061,422 (GRCm39)	missense	possibly damaging	0.75
R8963:Zfp938	UTSW	10	82,061,287 (GRCm39)	missense	possibly damaging	0.61
X0066:Zfp938	UTSW	10	82,061,931 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- ATCTCATTTACTATCCTTCAGGGG -3'
(R):5'- CCTTTGCACGTAACAGCCATG -3'

Sequencing Primer
(F):5'- TACCAAGATGTGCAAAGGATATACC -3'
(R):5'- CAGCCATGTTAAAATGCATGAAAG -3'

Posted On

2018-06-22