Mutagenetix > Incidental Mutation

Incidental Mutation 'R6995:Vmn2r50'

544122

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r50

Ensembl Gene

ENSMUSG00000094606

Gene Name

vomeronasal 2, receptor 50

Synonyms

EG434117

MMRRC Submission

045101-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.117) question?

Stock #

R6995 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

9771162-9787105 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 9779964 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 472 (Y472*)

Ref Sequence

ENSEMBL: ENSMUSP00000083478 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074943] [ENSMUST00000086298]

AlphaFold

E9PW61

Predicted Effect

probably null
Transcript: ENSMUST00000074943
AA Change: Y488*

SMART Domains

Protein: ENSMUSP00000074476
Gene: ENSMUSG00000094606
AA Change: Y488*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	469	1.4e-32	PFAM
Pfam:NCD3G	512	565	2.9e-20	PFAM
Pfam:7tm_3	597	833	1.3e-55	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000086298
AA Change: Y472*

SMART Domains

Protein: ENSMUSP00000083478
Gene: ENSMUSG00000094606
AA Change: Y472*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	452	7e-31	PFAM
Pfam:NCD3G	496	549	5.3e-19	PFAM
Pfam:7tm_3	579	818	3.9e-78	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930433I11Rik	A	G	7: 40,644,149 (GRCm39)	Y606C	probably benign	Het
Acad9	A	G	3: 36,139,630 (GRCm39)	Y410C	probably damaging	Het
Acot6	C	T	12: 84,156,149 (GRCm39)	P366S	probably damaging	Het
Adrm1b	T	C	3: 92,336,315 (GRCm39)		probably benign	Het
Anks1	G	T	17: 28,273,273 (GRCm39)	G964V	probably damaging	Het
Apol7a	A	T	15: 77,274,176 (GRCm39)		probably benign	Het
Arid3c	G	A	4: 41,725,087 (GRCm39)	A320V	probably damaging	Het
Bnip3l	A	T	14: 67,237,101 (GRCm39)	N50K	probably benign	Het
C1qtnf4	C	A	2: 90,720,297 (GRCm39)	A190D	probably benign	Het
Calhm5	A	T	10: 33,972,189 (GRCm39)	M82K	probably benign	Het
Cdcp3	G	T	7: 130,824,400 (GRCm39)	W165L	probably damaging	Het
Cdh1	G	T	8: 107,387,545 (GRCm39)	V482L	probably benign	Het
Cdrt4	A	C	11: 62,883,486 (GRCm39)	I63L	probably benign	Het
Cic	A	T	7: 24,970,736 (GRCm39)	T156S	possibly damaging	Het
Ckm	A	G	7: 19,154,156 (GRCm39)	N301S	probably benign	Het
Colgalt1	C	T	8: 72,076,165 (GRCm39)	R539C	probably damaging	Het
Cop1	A	G	1: 159,134,154 (GRCm39)	D132G	probably damaging	Het
Cpd	G	T	11: 76,675,881 (GRCm39)	N1257K	probably benign	Het
Cyp2c67	T	C	19: 39,604,123 (GRCm39)	H411R	probably damaging	Het
Cyp2r1	A	T	7: 114,152,316 (GRCm39)	F5I	probably damaging	Het
Dclk3	T	A	9: 111,296,768 (GRCm39)	L104Q	possibly damaging	Het
Dnah7c	A	T	1: 46,494,973 (GRCm39)	Q67L	probably benign	Het
Dnm1l	C	A	16: 16,147,671 (GRCm39)	E365*	probably null	Het
Dst	A	T	1: 34,205,315 (GRCm39)	I502F	probably damaging	Het
Espl1	T	C	15: 102,212,535 (GRCm39)	V547A	possibly damaging	Het
Foxa1	C	T	12: 57,589,264 (GRCm39)	A319T	probably benign	Het
Frem1	G	T	4: 82,904,838 (GRCm39)	H859N	probably damaging	Het
Frem3	T	A	8: 81,339,208 (GRCm39)	D500E	probably damaging	Het
Gpatch2l	G	A	12: 86,290,958 (GRCm39)	R47H	probably damaging	Het
Grip1	A	G	10: 119,822,375 (GRCm39)	D394G	probably damaging	Het
Gsap	C	A	5: 21,476,235 (GRCm39)	T589K	possibly damaging	Het
Gsg1l	A	G	7: 125,522,658 (GRCm39)	V190A	probably damaging	Het
Kcnmb1	A	T	11: 33,920,131 (GRCm39)	T115S	probably benign	Het
Kirrel2	A	C	7: 30,154,604 (GRCm39)	F169C	probably damaging	Het
L3mbtl1	C	T	2: 162,803,368 (GRCm39)	T397M	probably damaging	Het
Lama1	G	A	17: 68,060,820 (GRCm39)	C716Y		Het
Lamc2	G	A	1: 153,012,508 (GRCm39)	T722M	probably benign	Het
Lrig1	T	C	6: 94,588,610 (GRCm39)	D513G	possibly damaging	Het
Lrrk1	T	C	7: 65,942,090 (GRCm39)	D716G	probably damaging	Het
Matn4	G	A	2: 164,231,584 (GRCm39)	R582*	probably null	Het
Mdn1	A	T	4: 32,733,374 (GRCm39)	R3133W	probably benign	Het
Mmp7	A	G	9: 7,695,489 (GRCm39)	D122G	probably damaging	Het
Naaladl1	A	G	19: 6,165,578 (GRCm39)	D744G	possibly damaging	Het
Nampt	T	G	12: 32,898,742 (GRCm39)	Y453D	probably benign	Het
Nlrp12	A	G	7: 3,288,481 (GRCm39)	V677A	probably benign	Het
Ntng2	T	C	2: 29,087,080 (GRCm39)	N414S	probably damaging	Het
Or1e16	A	G	11: 73,286,410 (GRCm39)	V146A	probably benign	Het
Or4g17	A	G	2: 111,209,708 (GRCm39)	D121G	probably damaging	Het
Or5p52	A	G	7: 107,502,829 (GRCm39)	R302G	probably benign	Het
Or9m1b	A	C	2: 87,836,529 (GRCm39)	F189V	probably benign	Het
Parp4	A	C	14: 56,851,196 (GRCm39)	Q733P	probably damaging	Het
Pdcl3	G	A	1: 39,034,417 (GRCm39)	V56I	probably benign	Het
Pepd	A	G	7: 34,721,144 (GRCm39)	Y256C	probably damaging	Het
Plg	G	T	17: 12,637,938 (GRCm39)	R788L	probably benign	Het
Plod1	A	T	4: 148,000,675 (GRCm39)		probably benign	Het
Ptprc	A	G	1: 138,016,482 (GRCm39)	V513A	probably damaging	Het
Pxdn	T	C	12: 30,045,370 (GRCm39)	I373T	possibly damaging	Het
Qrfprl	A	T	6: 65,418,285 (GRCm39)	K151I	probably damaging	Het
Rbbp8	G	A	18: 11,851,965 (GRCm39)	G262E	probably damaging	Het
Rfc2	T	A	5: 134,623,104 (GRCm39)	Y240*	probably null	Het
Rps15	A	G	10: 80,129,598 (GRCm39)	E71G	possibly damaging	Het
Rsrc1	C	T	3: 66,901,982 (GRCm39)	P44L	unknown	Het
Ryr1	A	C	7: 28,793,607 (GRCm39)	M1287R	probably damaging	Het
Scart2	G	A	7: 139,841,514 (GRCm39)	D273N	probably benign	Het
Serpina9	A	G	12: 103,967,495 (GRCm39)	L300P	probably damaging	Het
Sh3rf2	G	A	18: 42,234,606 (GRCm39)	A130T	probably damaging	Het
Slc12a8	G	A	16: 33,355,263 (GRCm39)	W26*	probably null	Het
Snx18	A	T	13: 113,731,265 (GRCm39)	H576Q	probably damaging	Het
Tagln3	G	A	16: 45,543,321 (GRCm39)	T107I	probably benign	Het
Thap1	C	A	8: 26,652,679 (GRCm39)	T139K	probably damaging	Het
Tle4	T	A	19: 14,541,817 (GRCm39)		probably null	Het
Tmem87a	A	T	2: 120,193,409 (GRCm39)	M502K	possibly damaging	Het
Tpcn2	A	T	7: 144,810,522 (GRCm39)	V605E	probably benign	Het
Trgj1	C	A	13: 19,394,529 (GRCm39)		probably benign	Het
Tsc2	A	T	17: 24,847,028 (GRCm39)	V179E	probably damaging	Het
Ubap2l	T	C	3: 89,916,548 (GRCm39)	T909A	probably damaging	Het
Ubl4b	T	G	3: 107,462,140 (GRCm39)	Q40P	probably damaging	Het
Vmn1r229	A	G	17: 21,035,277 (GRCm39)	D174G	probably damaging	Het
Vmn2r74	T	A	7: 85,601,943 (GRCm39)	D565V	probably benign	Het
Vmn2r74	T	A	7: 85,606,860 (GRCm39)		probably null	Het
Vmn2r82	T	A	10: 79,232,377 (GRCm39)	V792E	probably damaging	Het
Zfp335	G	A	2: 164,735,210 (GRCm39)	Q1179*	probably null	Het
Zmynd12	A	C	4: 119,310,772 (GRCm39)	K327Q	probably benign	Het
Zwilch	G	A	9: 64,072,731 (GRCm39)	Q27*	probably null	Het

Other mutations in Vmn2r50

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01536:Vmn2r50	APN	7	9,771,610 (GRCm39)	missense	probably damaging	1.00
IGL01739:Vmn2r50	APN	7	9,771,364 (GRCm39)	missense	probably damaging	1.00
IGL02351:Vmn2r50	APN	7	9,787,002 (GRCm39)	missense	probably benign	0.01
IGL02358:Vmn2r50	APN	7	9,787,002 (GRCm39)	missense	probably benign	0.01
IGL02871:Vmn2r50	APN	7	9,781,714 (GRCm39)	missense	possibly damaging	0.91
IGL02962:Vmn2r50	APN	7	9,784,252 (GRCm39)	missense	probably damaging	1.00
IGL03187:Vmn2r50	APN	7	9,771,368 (GRCm39)	missense	probably damaging	1.00
IGL03346:Vmn2r50	APN	7	9,779,929 (GRCm39)	missense	probably damaging	0.99
PIT4651001:Vmn2r50	UTSW	7	9,771,659 (GRCm39)	missense	probably benign	0.30
R0530:Vmn2r50	UTSW	7	9,781,644 (GRCm39)	missense	possibly damaging	0.82
R1291:Vmn2r50	UTSW	7	9,771,404 (GRCm39)	missense	probably damaging	0.98
R1438:Vmn2r50	UTSW	7	9,784,062 (GRCm39)	nonsense	probably null
R1713:Vmn2r50	UTSW	7	9,771,731 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r50	UTSW	7	9,781,605 (GRCm39)	missense	probably benign	0.20
R1750:Vmn2r50	UTSW	7	9,786,915 (GRCm39)	missense	possibly damaging	0.69
R1918:Vmn2r50	UTSW	7	9,781,610 (GRCm39)	missense	probably benign	0.03
R2435:Vmn2r50	UTSW	7	9,787,026 (GRCm39)	missense	probably benign	0.27
R2511:Vmn2r50	UTSW	7	9,781,640 (GRCm39)	missense	possibly damaging	0.81
R3795:Vmn2r50	UTSW	7	9,771,851 (GRCm39)	missense	probably benign	0.18
R4156:Vmn2r50	UTSW	7	9,774,309 (GRCm39)	missense	probably benign	0.12
R4332:Vmn2r50	UTSW	7	9,786,922 (GRCm39)	missense	probably benign	0.32
R4399:Vmn2r50	UTSW	7	9,781,834 (GRCm39)	missense	possibly damaging	0.81
R4411:Vmn2r50	UTSW	7	9,784,235 (GRCm39)	missense	probably damaging	0.99
R4412:Vmn2r50	UTSW	7	9,784,235 (GRCm39)	missense	probably damaging	0.99
R4413:Vmn2r50	UTSW	7	9,784,235 (GRCm39)	missense	probably damaging	0.99
R4645:Vmn2r50	UTSW	7	9,771,162 (GRCm39)	makesense	probably null
R5151:Vmn2r50	UTSW	7	9,786,970 (GRCm39)	missense	probably benign	0.00
R5175:Vmn2r50	UTSW	7	9,771,644 (GRCm39)	missense	probably damaging	1.00
R5291:Vmn2r50	UTSW	7	9,781,752 (GRCm39)	missense	probably damaging	1.00
R5457:Vmn2r50	UTSW	7	9,781,873 (GRCm39)	missense	probably damaging	0.98
R5559:Vmn2r50	UTSW	7	9,771,253 (GRCm39)	missense	probably damaging	1.00
R5579:Vmn2r50	UTSW	7	9,784,016 (GRCm39)	missense	probably benign	0.00
R5711:Vmn2r50	UTSW	7	9,774,299 (GRCm39)	missense	possibly damaging	0.81
R5759:Vmn2r50	UTSW	7	9,781,905 (GRCm39)	missense	probably damaging	1.00
R6004:Vmn2r50	UTSW	7	9,783,986 (GRCm39)	missense	probably benign	0.00
R6394:Vmn2r50	UTSW	7	9,774,253 (GRCm39)	missense	probably damaging	1.00
R6488:Vmn2r50	UTSW	7	9,771,644 (GRCm39)	missense	probably damaging	1.00
R6762:Vmn2r50	UTSW	7	9,787,010 (GRCm39)	missense	probably benign	0.04
R6998:Vmn2r50	UTSW	7	9,771,684 (GRCm39)	missense	probably benign	0.03
R7019:Vmn2r50	UTSW	7	9,784,172 (GRCm39)	missense	probably benign	0.01
R7027:Vmn2r50	UTSW	7	9,781,539 (GRCm39)	missense	probably damaging	1.00
R7231:Vmn2r50	UTSW	7	9,787,010 (GRCm39)	missense	probably benign	0.04
R7343:Vmn2r50	UTSW	7	9,784,277 (GRCm39)	critical splice acceptor site	probably null
R7554:Vmn2r50	UTSW	7	9,784,066 (GRCm39)	missense	probably null	0.00
R7704:Vmn2r50	UTSW	7	9,781,665 (GRCm39)	missense	probably benign	0.05
R7768:Vmn2r50	UTSW	7	9,771,298 (GRCm39)	missense	probably damaging	0.99
R7773:Vmn2r50	UTSW	7	9,771,562 (GRCm39)	missense	possibly damaging	0.70
R7975:Vmn2r50	UTSW	7	9,771,272 (GRCm39)	missense	probably benign	0.39
R7987:Vmn2r50	UTSW	7	9,772,016 (GRCm39)	missense	probably benign	0.14
R7996:Vmn2r50	UTSW	7	9,781,795 (GRCm39)	missense	probably damaging	0.99
R8062:Vmn2r50	UTSW	7	9,774,240 (GRCm39)	critical splice donor site	probably null
R8396:Vmn2r50	UTSW	7	9,781,639 (GRCm39)	nonsense	probably null
R8466:Vmn2r50	UTSW	7	9,783,997 (GRCm39)	missense	probably damaging	0.97
R8985:Vmn2r50	UTSW	7	9,779,974 (GRCm39)	missense	probably damaging	1.00
R9068:Vmn2r50	UTSW	7	9,772,061 (GRCm39)	missense	possibly damaging	0.46
R9155:Vmn2r50	UTSW	7	9,781,571 (GRCm39)	missense	probably damaging	1.00
R9238:Vmn2r50	UTSW	7	9,781,503 (GRCm39)	missense	probably benign	0.01
R9576:Vmn2r50	UTSW	7	9,771,190 (GRCm39)	missense	probably benign
R9626:Vmn2r50	UTSW	7	9,771,960 (GRCm39)	nonsense	probably null
R9631:Vmn2r50	UTSW	7	9,786,990 (GRCm39)	nonsense	probably null
X0067:Vmn2r50	UTSW	7	9,786,954 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r50	UTSW	7	9,780,086 (GRCm39)	missense	probably benign	0.01
Z1088:Vmn2r50	UTSW	7	9,771,427 (GRCm39)	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- CTGTTCCAGAGGTTTAATTAAGGG -3'
(R):5'- CAGAATGCATTTTATGACTTGAAGG -3'

Sequencing Primer
(F):5'- TTCCAGAGGTTTAATTAAGGGAGGAG -3'
(R):5'- CTCTTGGGGACAAAGTGA -3'

Posted On

2019-05-13