Mutagenetix > Incidental Mutation

Incidental Mutation 'R0583:Zfta'

56541

Institutional Source

Beutler Lab

Gene Symbol

Zfta

Ensembl Gene

ENSMUSG00000053080

Gene Name

zinc finger translocation associated

Synonyms

2700081O15Rik

MMRRC Submission

038773-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R0583 (G1)

Quality Score

165

Status

Not validated

Chromosome

Chromosomal Location

7394990-7403269 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 7397639 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 62 (D62G)

Ref Sequence

ENSEMBL: ENSMUSP00000139847 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159348] [ENSMUST00000161907]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000159346

SMART Domains

Protein: ENSMUSP00000124442
Gene: ENSMUSG00000053080

Domain	Start	End	E-Value	Type
coiled coil region	14	41	N/A	INTRINSIC
low complexity region	42	67	N/A	INTRINSIC
low complexity region	72	97	N/A	INTRINSIC
low complexity region	178	215	N/A	INTRINSIC
ZnF_C2H2	231	256	2.06e1	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000159348
AA Change: D116G

SMART Domains

Protein: ENSMUSP00000123773
Gene: ENSMUSG00000053080
AA Change: D116G

Domain	Start	End	E-Value	Type
low complexity region	3	20	N/A	INTRINSIC
low complexity region	46	58	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	99	108	N/A	INTRINSIC
ZnF_C2H2	124	149	3.38e1	SMART
low complexity region	171	201	N/A	INTRINSIC
low complexity region	205	230	N/A	INTRINSIC
low complexity region	235	260	N/A	INTRINSIC
ZnF_C2H2	283	308	1.06e2	SMART
low complexity region	382	419	N/A	INTRINSIC
ZnF_C2H2	435	460	2.06e1	SMART
low complexity region	491	522	N/A	INTRINSIC
low complexity region	540	547	N/A	INTRINSIC
low complexity region	557	572	N/A	INTRINSIC
ZnF_C2H2	600	625	2.54e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160561

SMART Domains

Protein: ENSMUSP00000124645
Gene: ENSMUSG00000053080

Domain	Start	End	E-Value	Type
ZnF_C2H2	10	35	1.06e2	SMART
low complexity region	109	146	N/A	INTRINSIC
ZnF_C2H2	162	187	2.06e1	SMART
low complexity region	218	249	N/A	INTRINSIC
low complexity region	267	274	N/A	INTRINSIC
low complexity region	284	299	N/A	INTRINSIC
ZnF_C2H2	327	352	2.54e1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000161907
AA Change: D62G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000139847
Gene: ENSMUSG00000053080
AA Change: D62G

Domain	Start	End	E-Value	Type
low complexity region	12	24	N/A	INTRINSIC
low complexity region	45	54	N/A	INTRINSIC
ZnF_C2H2	70	95	1.4e-1	SMART
coiled coil region	124	151	N/A	INTRINSIC
low complexity region	173	180	N/A	INTRINSIC
low complexity region	190	205	N/A	INTRINSIC
ZnF_C2H2	233	258	1.1e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183837

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000189779

Predicted Effect

probably benign
Transcript: ENSMUST00000191580

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.1%
20x: 93.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	A	G	9: 57,164,926 (GRCm39)	S483P	probably benign	Het
5730480H06Rik	A	G	5: 48,537,470 (GRCm39)	H169R	probably damaging	Het
Actn1	T	A	12: 80,245,803 (GRCm39)	I127F	probably damaging	Het
Cadm3	T	G	1: 173,168,738 (GRCm39)	T277P	probably benign	Het
Cast	T	C	13: 74,861,797 (GRCm39)	T629A	probably damaging	Het
Cblc	C	A	7: 19,526,486 (GRCm39)	C201F	probably benign	Het
Ccdc154	T	A	17: 25,387,398 (GRCm39)	D375E	possibly damaging	Het
Cdk6	A	G	5: 3,523,183 (GRCm39)	D201G	probably damaging	Het
Cep95	T	C	11: 106,705,449 (GRCm39)	V478A	probably benign	Het
Ciita	T	C	16: 10,341,668 (GRCm39)		probably null	Het
Clec4e	A	G	6: 123,260,653 (GRCm39)	F135S	probably damaging	Het
Cntn6	A	G	6: 104,753,275 (GRCm39)	D337G	possibly damaging	Het
Crlf3	A	T	11: 79,950,107 (GRCm39)	H174Q	probably damaging	Het
Cyb5r1	T	A	1: 134,335,339 (GRCm39)	F93I	probably damaging	Het
Dop1b	T	C	16: 93,552,374 (GRCm39)	I271T	probably benign	Het
Duxf1	G	A	10: 58,059,210 (GRCm39)	L515F	probably damaging	Het
Fcho1	T	C	8: 72,168,369 (GRCm39)	Y218C	probably damaging	Het
Fhad1	C	T	4: 141,631,301 (GRCm39)	M1297I	probably benign	Het
Igdcc4	T	C	9: 65,029,095 (GRCm39)	V244A	possibly damaging	Het
Ikzf5	A	G	7: 130,993,514 (GRCm39)		probably null	Het
Ilvbl	T	A	10: 78,419,101 (GRCm39)	V450E	probably damaging	Het
Kcns3	T	G	12: 11,141,479 (GRCm39)	N407H	probably damaging	Het
Klhl11	T	C	11: 100,355,150 (GRCm39)	K224E	possibly damaging	Het
Klra17	T	A	6: 129,845,656 (GRCm39)	D186V	probably damaging	Het
Lrrc37a	T	C	11: 103,389,263 (GRCm39)	D2054G	probably benign	Het
Mef2a	G	T	7: 66,884,896 (GRCm39)	S406*	probably null	Het
Mrgbp	A	G	2: 180,226,239 (GRCm39)	N104S	probably benign	Het
Mroh2a	GT	GTT	1: 88,183,888 (GRCm39)		probably null	Het
Muc5ac	C	A	7: 141,361,345 (GRCm39)	T1552N	probably damaging	Het
Muc5b	T	A	7: 141,410,435 (GRCm39)	Y1269*	probably null	Het
Myef2	T	C	2: 124,939,901 (GRCm39)		probably null	Het
Myg1	C	T	15: 102,246,225 (GRCm39)	Q367*	probably null	Het
Nalcn	T	C	14: 123,531,755 (GRCm39)	N1365S	possibly damaging	Het
Nfu1	T	C	6: 86,986,934 (GRCm39)	C18R	probably benign	Het
Nkx2-6	A	T	14: 69,412,228 (GRCm39)	Q132L	probably damaging	Het
Or10a3b	C	T	7: 108,444,621 (GRCm39)	A199T	possibly damaging	Het
Or8k38	T	A	2: 86,488,704 (GRCm39)	I33F	probably benign	Het
Pak3	TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	TTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	X: 142,526,889 (GRCm39)		probably benign	Het
Prh1	A	T	6: 132,548,796 (GRCm39)	Q101L	unknown	Het
Ribc2	A	T	15: 85,017,115 (GRCm39)		probably null	Het
Rnf19a	C	A	15: 36,253,151 (GRCm39)	R396L	probably damaging	Het
Sdad1	A	G	5: 92,452,923 (GRCm39)	I105T	probably damaging	Het
Sec24b	G	T	3: 129,834,960 (GRCm39)	Y79*	probably null	Het
Tatdn2	A	G	6: 113,679,486 (GRCm39)	E277G	possibly damaging	Het
Tex10	A	C	4: 48,451,952 (GRCm39)	F725V	probably damaging	Het
Themis3	T	C	17: 66,866,748 (GRCm39)	D164G	probably benign	Het
Ubxn7	T	C	16: 32,194,732 (GRCm39)	W220R	probably damaging	Het
Usp33	C	A	3: 152,073,891 (GRCm39)	R246S	probably damaging	Het
Vmn2r102	T	A	17: 19,897,043 (GRCm39)	V130E	probably benign	Het
Vmn2r112	C	T	17: 22,837,930 (GRCm39)	P797L	probably damaging	Het
Vmn2r114	ATTT	ATT	17: 23,509,906 (GRCm39)		probably null	Het
Yme1l1	T	C	2: 23,076,262 (GRCm39)	V340A	probably damaging	Het

Other mutations in Zfta

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02376:Zfta	APN	19	7,399,741 (GRCm39)	missense	probably damaging	1.00
IGL02814:Zfta	APN	19	7,397,787 (GRCm39)	missense	possibly damaging	0.94
H8562:Zfta	UTSW	19	7,400,286 (GRCm39)	missense	probably benign	0.01
R0034:Zfta	UTSW	19	7,397,724 (GRCm39)	missense	probably damaging	1.00
R2351:Zfta	UTSW	19	7,399,609 (GRCm39)	missense	probably damaging	1.00
R4326:Zfta	UTSW	19	7,398,591 (GRCm39)	intron	probably benign
R4329:Zfta	UTSW	19	7,398,591 (GRCm39)	intron	probably benign
R5474:Zfta	UTSW	19	7,397,524 (GRCm39)	missense	probably damaging	1.00
R5735:Zfta	UTSW	19	7,400,161 (GRCm39)	missense	probably benign
R6168:Zfta	UTSW	19	7,400,305 (GRCm39)	missense	probably benign	0.40
R6739:Zfta	UTSW	19	7,398,712 (GRCm39)	nonsense	probably null
R7780:Zfta	UTSW	19	7,399,737 (GRCm39)	missense	probably damaging	0.96
R7855:Zfta	UTSW	19	7,399,621 (GRCm39)	missense	probably damaging	1.00
R8550:Zfta	UTSW	19	7,400,320 (GRCm39)	missense	probably benign	0.00
R9423:Zfta	UTSW	19	7,397,624 (GRCm39)	missense	probably damaging	1.00
R9429:Zfta	UTSW	19	7,399,594 (GRCm39)	missense	probably damaging	1.00
Z1176:Zfta	UTSW	19	7,400,112 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CTTTCATTGCTCTTGCCACCCCTCT -3'
(R):5'- CCCTTGCCTCTCCATATTCTGTATTACCT -3'

Sequencing Primer
(F):5'- TAGGAAATTGAGAGCCTGTCTCC -3'
(R):5'- cccctcctcctcctcttc -3'

Posted On

2013-07-11