Mutagenetix > Incidental Mutation

Incidental Mutation 'R0584:Ccl25'

56557

Institutional Source

Beutler Lab

Gene Symbol

Ccl25

Ensembl Gene

ENSMUSG00000023235

Gene Name

C-C motif chemokine ligand 25

Synonyms

Scya25, CKb15, TECK

MMRRC Submission

038774-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R0584 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

4375210-4410020 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

C to T at 4404085 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000140747 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024004] [ENSMUST00000110982] [ENSMUST00000127460] [ENSMUST00000136191] [ENSMUST00000155797]

AlphaFold

O35903

Predicted Effect

probably benign
Transcript: ENSMUST00000024004

SMART Domains

Protein: ENSMUSP00000024004
Gene: ENSMUSG00000023235

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
SCY	27	88	1.34e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000110982

SMART Domains

Protein: ENSMUSP00000106610
Gene: ENSMUSG00000023235

Domain	Start	End	E-Value	Type
SCY	27	88	1.34e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000127460

SMART Domains

Protein: ENSMUSP00000120719
Gene: ENSMUSG00000023235

Domain	Start	End	E-Value	Type
transmembrane domain	87	109	N/A	INTRINSIC
SCY	111	172	1.34e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000136191

SMART Domains

Protein: ENSMUSP00000117515
Gene: ENSMUSG00000023235

Domain	Start	End	E-Value	Type
Pfam:IL8	23	66	2.3e-8	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000155797

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.2%
20x: 93.7%

Validation Efficiency

98% (47/48)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired accumulation of antigen-specific CD8+ T lymphocytes within both lamina propria and epithelium of the small intestine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	T	C	10: 79,847,564 (GRCm39)	S1745P	probably damaging	Het
Agfg2	G	A	5: 137,665,992 (GRCm39)	T89I	probably damaging	Het
Agtr1a	A	G	13: 30,565,017 (GRCm39)	I27M	probably damaging	Het
Armh1	A	T	4: 117,087,047 (GRCm39)	L206Q	probably damaging	Het
Asxl2	A	T	12: 3,546,632 (GRCm39)	E472V	probably damaging	Het
Atp2c2	T	A	8: 120,465,157 (GRCm39)	V313E	probably benign	Het
Casp12	A	G	9: 5,352,268 (GRCm39)	I87V	probably null	Het
Col9a1	T	C	1: 24,263,571 (GRCm39)		probably benign	Het
Cyth4	A	G	15: 78,494,078 (GRCm39)		probably null	Het
Dnah9	A	G	11: 65,881,315 (GRCm39)	Y2587H	probably damaging	Het
Dppa3	T	C	6: 122,606,951 (GRCm39)	I147T	probably benign	Het
Fam120b	T	C	17: 15,622,384 (GRCm39)	S121P	probably damaging	Het
Fam209	C	T	2: 172,316,081 (GRCm39)	T152M	probably benign	Het
Fam98a	A	G	17: 75,851,772 (GRCm39)	L103P	probably damaging	Het
Fcho1	T	C	8: 72,168,369 (GRCm39)	Y218C	probably damaging	Het
Fitm1	T	C	14: 55,814,113 (GRCm39)	V203A	probably benign	Het
Gcn1	G	A	5: 115,733,074 (GRCm39)	R1037Q	probably damaging	Het
Gm11563	A	G	11: 99,549,451 (GRCm39)	I101T	unknown	Het
Gpd1l	A	G	9: 114,743,412 (GRCm39)	F163L	probably damaging	Het
Grp	C	A	18: 66,006,766 (GRCm39)	A30E	possibly damaging	Het
H1f7	C	A	15: 98,154,958 (GRCm39)	E64*	probably null	Het
Icosl	T	C	10: 77,907,709 (GRCm39)	Y90H	possibly damaging	Het
Itsn2	A	G	12: 4,747,180 (GRCm39)	T1194A	probably benign	Het
Lrp6	A	G	6: 134,433,039 (GRCm39)	S1431P	probably damaging	Het
Ltbp1	T	C	17: 75,670,467 (GRCm39)	S1503P	probably damaging	Het
Mef2a	G	T	7: 66,884,896 (GRCm39)	S406*	probably null	Het
Myh13	G	T	11: 67,251,200 (GRCm39)	E1360*	probably null	Het
Nop58	A	G	1: 59,745,919 (GRCm39)	D400G	probably benign	Het
Or5p79	T	C	7: 108,221,622 (GRCm39)	L201P	probably benign	Het
Or6c205	T	C	10: 129,086,817 (GRCm39)	V138A	probably benign	Het
Oscp1	A	G	4: 125,977,387 (GRCm39)		probably null	Het
Pde9a	A	T	17: 31,678,951 (GRCm39)	Y264F	probably damaging	Het
Pkhd1	T	A	1: 20,309,660 (GRCm39)	K2763*	probably null	Het
Prkci	T	A	3: 31,079,289 (GRCm39)	C42*	probably null	Het
Ptprr	T	C	10: 116,087,063 (GRCm39)	V270A	probably damaging	Het
Rsf1	T	A	7: 97,311,335 (GRCm39)	N688K	possibly damaging	Het
S1pr3	A	T	13: 51,573,697 (GRCm39)	M293L	probably benign	Het
Scn10a	A	C	9: 119,499,597 (GRCm39)	L232R	probably damaging	Het
Sirt5	T	A	13: 43,548,204 (GRCm39)		probably null	Het
Sp6	C	T	11: 96,913,091 (GRCm39)	T268M	probably damaging	Het
Spag5	A	G	11: 78,194,921 (GRCm39)	N76S	possibly damaging	Het
Tecta	T	C	9: 42,259,204 (GRCm39)	N1560D	possibly damaging	Het
Togaram1	T	C	12: 65,014,279 (GRCm39)	L510P	probably damaging	Het
Umps	A	G	16: 33,779,494 (GRCm39)	I401T	probably damaging	Het
Vars2	A	G	17: 35,977,578 (GRCm39)	V118A	possibly damaging	Het

Other mutations in Ccl25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02070:Ccl25	APN	8	4,398,700 (GRCm39)	intron	probably benign
IGL02188:Ccl25	APN	8	4,398,552 (GRCm39)	intron	probably benign
IGL03338:Ccl25	APN	8	4,399,898 (GRCm39)	intron	probably benign
R0613:Ccl25	UTSW	8	4,399,850 (GRCm39)	missense	probably benign	0.42
R1208:Ccl25	UTSW	8	4,407,631 (GRCm39)	missense	possibly damaging	0.92
R1208:Ccl25	UTSW	8	4,407,631 (GRCm39)	missense	possibly damaging	0.92
R1413:Ccl25	UTSW	8	4,403,892 (GRCm39)	makesense	probably null
R3844:Ccl25	UTSW	8	4,404,183 (GRCm39)	missense	possibly damaging	0.86
R4279:Ccl25	UTSW	8	4,399,829 (GRCm39)	missense	probably damaging	1.00
R4921:Ccl25	UTSW	8	4,403,913 (GRCm39)	missense	possibly damaging	0.92
R7021:Ccl25	UTSW	8	4,399,641 (GRCm39)	intron	probably benign
R7033:Ccl25	UTSW	8	4,399,641 (GRCm39)	intron	probably benign
R7630:Ccl25	UTSW	8	4,403,955 (GRCm39)	missense	probably damaging	1.00
R8317:Ccl25	UTSW	8	4,404,138 (GRCm39)	missense	probably benign	0.00
R8550:Ccl25	UTSW	8	4,377,890 (GRCm39)	missense	possibly damaging	0.72
R9799:Ccl25	UTSW	8	4,377,799 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGGGTTACCAGCACAGGATCAAATG -3'
(R):5'- GCTGAAGGACTCAATGCACAATGC -3'

Sequencing Primer
(F):5'- CCAGCACAGGATCAAATGGAATG -3'
(R):5'- CTCAATGCACAATGCTAAAAGGG -3'

Posted On

2013-07-11