Incidental Mutation 'R0853:Tbx20'
| ID |
82619 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Tbx20
|
| Ensembl Gene |
ENSMUSG00000031965 |
| Gene Name |
T-box 20 |
| Synonyms |
Tbx12, 9430010M06Rik |
| MMRRC Submission |
039032-MU
|
| Accession Numbers |
|
| Essential gene? |
Essential
(E-score: 1.000)
|
| Stock # |
R0853 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
9 |
| Chromosomal Location |
24632108-24685580 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 24636908 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Methionine to Threonine
at position 393
(M393T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000052591
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000052946]
|
| AlphaFold |
Q9ES03 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000052946
AA Change: M393T
PolyPhen 2
Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)
|
| SMART Domains |
Protein: ENSMUSP00000052591
Gene: ENSMUSG00000031965
AA Change: M393T
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
68 |
78 |
N/A |
INTRINSIC |
|
low complexity region
|
81 |
95 |
N/A |
INTRINSIC |
|
TBOX
|
98 |
292 |
1.08e-122 |
SMART |
|
low complexity region
|
339 |
353 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.0590 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.1%
- 20x: 93.9%
|
| Validation Efficiency |
93% (42/45) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
PHENOTYPE: Homozygous null mice display embryonic lethality, impaired cardiac looping, a small hourglass shaped heart, and decreased cardiomyocyte proliferation. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 43 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Amotl1 |
G |
T |
9: 14,504,074 (GRCm39) |
P378Q |
probably damaging |
Het |
| Angpt4 |
A |
G |
2: 151,780,847 (GRCm39) |
E365G |
probably damaging |
Het |
| Asb6 |
G |
A |
2: 30,717,042 (GRCm39) |
P61L |
possibly damaging |
Het |
| Atp8a2 |
T |
C |
14: 60,097,719 (GRCm39) |
K770E |
probably benign |
Het |
| Atxn7 |
C |
A |
14: 14,089,465 (GRCm38) |
|
probably benign |
Het |
| Cacul1 |
A |
T |
19: 60,522,664 (GRCm39) |
I290N |
probably damaging |
Het |
| Ccser2 |
A |
C |
14: 36,662,367 (GRCm39) |
S272R |
probably benign |
Het |
| Cfh |
T |
A |
1: 140,033,228 (GRCm39) |
H772L |
probably damaging |
Het |
| Cldn6 |
T |
G |
17: 23,900,438 (GRCm39) |
I134S |
probably damaging |
Het |
| Col3a1 |
T |
A |
1: 45,382,484 (GRCm39) |
|
probably benign |
Het |
| Fam118a |
T |
C |
15: 84,932,726 (GRCm39) |
F156S |
possibly damaging |
Het |
| Fgd4 |
T |
A |
16: 16,292,251 (GRCm39) |
|
probably benign |
Het |
| Gckr |
G |
C |
5: 31,462,392 (GRCm39) |
A242P |
probably damaging |
Het |
| Gcnt4 |
A |
G |
13: 97,083,343 (GRCm39) |
D213G |
probably damaging |
Het |
| Hace1 |
T |
A |
10: 45,524,779 (GRCm39) |
V237E |
probably damaging |
Het |
| Herc3 |
T |
C |
6: 58,853,549 (GRCm39) |
L570P |
probably damaging |
Het |
| Hk1 |
T |
A |
10: 62,107,495 (GRCm39) |
K827* |
probably null |
Het |
| Hyal6 |
T |
C |
6: 24,734,072 (GRCm39) |
F2L |
probably benign |
Het |
| Jak2 |
C |
A |
19: 29,262,326 (GRCm39) |
Y382* |
probably null |
Het |
| Kat8 |
C |
T |
7: 127,524,396 (GRCm39) |
H425Y |
probably benign |
Het |
| Kcnj3 |
T |
C |
2: 55,327,235 (GRCm39) |
F8S |
possibly damaging |
Het |
| Klk1 |
T |
A |
7: 43,870,922 (GRCm39) |
|
probably benign |
Het |
| Klra8 |
T |
C |
6: 130,095,977 (GRCm39) |
Y205C |
probably damaging |
Het |
| Kpnb1 |
T |
C |
11: 97,078,237 (GRCm39) |
E26G |
probably damaging |
Het |
| Mroh2a |
A |
G |
1: 88,186,386 (GRCm39) |
S64G |
probably benign |
Het |
| Naip2 |
T |
C |
13: 100,298,362 (GRCm39) |
E558G |
probably benign |
Het |
| Naip2 |
C |
T |
13: 100,298,368 (GRCm39) |
G556D |
probably benign |
Het |
| Nphp3 |
T |
C |
9: 103,909,132 (GRCm39) |
S781P |
probably benign |
Het |
| Nqo2 |
A |
T |
13: 34,163,560 (GRCm39) |
H73L |
probably benign |
Het |
| P3h3 |
G |
T |
6: 124,831,896 (GRCm39) |
D296E |
probably benign |
Het |
| Pah |
G |
A |
10: 87,412,080 (GRCm39) |
|
probably null |
Het |
| Pcdhb4 |
T |
G |
18: 37,442,938 (GRCm39) |
Y749* |
probably null |
Het |
| Pdgfrb |
C |
A |
18: 61,213,399 (GRCm39) |
N914K |
probably damaging |
Het |
| Ralyl |
A |
G |
3: 14,011,566 (GRCm39) |
Y4C |
probably damaging |
Het |
| Rapgef6 |
T |
A |
11: 54,559,503 (GRCm39) |
I1052N |
probably damaging |
Het |
| Sdk2 |
G |
A |
11: 113,712,241 (GRCm39) |
T1642I |
probably benign |
Het |
| Siglec1 |
G |
A |
2: 130,926,942 (GRCm39) |
T207M |
probably damaging |
Het |
| Taf1b |
T |
C |
12: 24,564,827 (GRCm39) |
L148P |
probably benign |
Het |
| Tdp1 |
G |
A |
12: 99,901,326 (GRCm39) |
R536H |
probably damaging |
Het |
| Tubgcp5 |
T |
A |
7: 55,464,599 (GRCm39) |
|
probably benign |
Het |
| Vezf1 |
A |
T |
11: 88,068,435 (GRCm38) |
|
probably benign |
Het |
| Vmn1r58 |
G |
T |
7: 5,413,324 (GRCm39) |
T302K |
probably damaging |
Het |
| Zfp1002 |
A |
T |
2: 150,097,398 (GRCm39) |
S38R |
probably benign |
Het |
|
| Other mutations in Tbx20 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00332:Tbx20
|
APN |
9 |
24,670,044 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00572:Tbx20
|
APN |
9 |
24,636,984 (GRCm39) |
missense |
probably benign |
|
|
IGL01016:Tbx20
|
APN |
9 |
24,661,617 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01317:Tbx20
|
APN |
9 |
24,681,051 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02643:Tbx20
|
APN |
9 |
24,685,009 (GRCm39) |
unclassified |
probably benign |
|
|
IGL02690:Tbx20
|
APN |
9 |
24,685,033 (GRCm39) |
missense |
probably benign |
0.27 |
|
BB006:Tbx20
|
UTSW |
9 |
24,637,059 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
BB016:Tbx20
|
UTSW |
9 |
24,637,059 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R0855:Tbx20
|
UTSW |
9 |
24,636,908 (GRCm39) |
missense |
probably benign |
0.05 |
|
R0856:Tbx20
|
UTSW |
9 |
24,636,908 (GRCm39) |
missense |
probably benign |
0.05 |
|
R1781:Tbx20
|
UTSW |
9 |
24,636,795 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1840:Tbx20
|
UTSW |
9 |
24,636,972 (GRCm39) |
missense |
probably benign |
0.22 |
|
R1981:Tbx20
|
UTSW |
9 |
24,682,209 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R2063:Tbx20
|
UTSW |
9 |
24,681,067 (GRCm39) |
nonsense |
probably null |
|
|
R2357:Tbx20
|
UTSW |
9 |
24,681,072 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R4166:Tbx20
|
UTSW |
9 |
24,681,040 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4790:Tbx20
|
UTSW |
9 |
24,637,010 (GRCm39) |
missense |
probably benign |
0.34 |
|
R4904:Tbx20
|
UTSW |
9 |
24,670,129 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R5436:Tbx20
|
UTSW |
9 |
24,681,016 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5799:Tbx20
|
UTSW |
9 |
24,636,816 (GRCm39) |
nonsense |
probably null |
|
|
R5898:Tbx20
|
UTSW |
9 |
24,670,155 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6914:Tbx20
|
UTSW |
9 |
24,636,779 (GRCm39) |
missense |
probably benign |
0.19 |
|
R6962:Tbx20
|
UTSW |
9 |
24,681,036 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7556:Tbx20
|
UTSW |
9 |
24,661,573 (GRCm39) |
splice site |
probably null |
|
|
R7731:Tbx20
|
UTSW |
9 |
24,681,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7741:Tbx20
|
UTSW |
9 |
24,651,581 (GRCm39) |
splice site |
probably null |
|
|
R7832:Tbx20
|
UTSW |
9 |
24,685,108 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7929:Tbx20
|
UTSW |
9 |
24,637,059 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R7982:Tbx20
|
UTSW |
9 |
24,685,220 (GRCm39) |
unclassified |
probably benign |
|
|
R8110:Tbx20
|
UTSW |
9 |
24,636,821 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8974:Tbx20
|
UTSW |
9 |
24,681,082 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9058:Tbx20
|
UTSW |
9 |
24,681,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTCCTGGAGAGTCCTTGCTCCAAT -3'
(R):5'- GTCTCTCAGTTCCTGGGTATCGTCA -3'
Sequencing Primer
(F):5'- CATAGCCATTTGCACACTCTG -3'
(R):5'- AGTTCCTGGGTATCGTCATCTTC -3'
|
| Posted On |
2013-11-08 |
Incidental Mutation