Mutagenetix > Incidental Mutation

Incidental Mutation 'R2242:Vmn2r57'

240697

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r57

Ensembl Gene

ENSMUSG00000066537

Gene Name

vomeronasal 2, receptor 57

Synonyms

EG269902

MMRRC Submission

040242-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.106) question?

Stock #

R2242 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41049156-41098065 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 41077498 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 223 (T223A)

Ref Sequence

ENSEMBL: ENSMUSP00000125817 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094532] [ENSMUST00000165029]

AlphaFold

L7N269

Predicted Effect

probably benign
Transcript: ENSMUST00000094532

Predicted Effect

probably benign
Transcript: ENSMUST00000165029
AA Change: T223A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000125817
Gene: ENSMUSG00000066537
AA Change: T223A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.4e-44	PFAM
Pfam:NCD3G	514	567	2.7e-23	PFAM
Pfam:7tm_3	600	835	1.8e-52	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy2	A	T	13: 68,837,460 (GRCm39)	S630T	probably benign	Het
Afap1l2	T	C	19: 56,902,900 (GRCm39)	I760V	possibly damaging	Het
Cdc20	A	G	4: 118,290,722 (GRCm39)	V426A	probably benign	Het
Clca3a2	T	C	3: 144,796,551 (GRCm39)	S219G	probably damaging	Het
Corin	A	T	5: 72,490,054 (GRCm39)	D603E	probably damaging	Het
Dctn1	A	G	6: 83,176,687 (GRCm39)	Y1205C	probably damaging	Het
Dync2h1	A	T	9: 7,037,828 (GRCm39)		probably null	Het
Dysf	G	A	6: 84,163,491 (GRCm39)		probably null	Het
Eif1ad	CGAGGAGGAGGAGGAGGAGG	CGAGGAGGAGGAGGAGG	19: 5,420,086 (GRCm39)		probably benign	Het
Fes	T	G	7: 80,031,473 (GRCm39)	E467A	probably damaging	Het
Ftsj3	T	A	11: 106,141,604 (GRCm39)	Q548L	probably benign	Het
Gpc6	A	T	14: 117,424,199 (GRCm39)	T96S	probably benign	Het
Hivep2	C	A	10: 14,004,713 (GRCm39)	T437K	probably benign	Het
Jarid2	T	A	13: 45,059,752 (GRCm39)	N661K	probably damaging	Het
Lama4	G	A	10: 38,902,689 (GRCm39)	C221Y	probably damaging	Het
Malrd1	T	C	2: 16,106,755 (GRCm39)	C1856R	unknown	Het
Mfsd6	G	T	1: 52,748,757 (GRCm39)	P36Q	probably benign	Het
Ofcc1	A	G	13: 40,296,263 (GRCm39)	S524P	probably benign	Het
Or13c3	A	G	4: 52,855,769 (GRCm39)	V248A	probably damaging	Het
Or2n1b	A	G	17: 38,459,613 (GRCm39)	I45V	possibly damaging	Het
Ripor2	A	G	13: 24,855,755 (GRCm39)	E65G	probably benign	Het
Sardh	A	T	2: 27,125,527 (GRCm39)	V329E	possibly damaging	Het
Slc37a3	A	G	6: 39,315,739 (GRCm39)	S446P	probably benign	Het
Wdr47	A	G	3: 108,526,431 (GRCm39)	D318G	probably damaging	Het
Zic4	C	T	9: 91,260,706 (GRCm39)		probably benign	Het

Other mutations in Vmn2r57

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Vmn2r57	APN	7	41,078,209 (GRCm39)	missense	probably benign
IGL01108:Vmn2r57	APN	7	41,077,008 (GRCm39)	missense	probably benign	0.01
IGL01112:Vmn2r57	APN	7	41,074,467 (GRCm39)	missense	probably damaging	1.00
IGL01516:Vmn2r57	APN	7	41,049,370 (GRCm39)	missense	probably damaging	1.00
IGL01880:Vmn2r57	APN	7	41,049,619 (GRCm39)	missense	possibly damaging	0.73
IGL02117:Vmn2r57	APN	7	41,049,874 (GRCm39)	missense	probably benign	0.00
IGL02500:Vmn2r57	APN	7	41,077,650 (GRCm39)	missense	probably benign
IGL02801:Vmn2r57	APN	7	41,098,056 (GRCm39)	missense	probably benign	0.13
IGL02993:Vmn2r57	APN	7	41,077,498 (GRCm39)	missense	probably benign	0.04
IGL02996:Vmn2r57	APN	7	41,049,165 (GRCm39)	missense	probably benign	0.02
R0008:Vmn2r57	UTSW	7	41,050,076 (GRCm39)	missense	probably damaging	1.00
R0032:Vmn2r57	UTSW	7	41,049,157 (GRCm39)	splice site	probably null
R0305:Vmn2r57	UTSW	7	41,076,967 (GRCm39)	missense	probably benign	0.00
R0469:Vmn2r57	UTSW	7	41,077,216 (GRCm39)	missense	possibly damaging	0.58
R0510:Vmn2r57	UTSW	7	41,077,216 (GRCm39)	missense	possibly damaging	0.58
R0847:Vmn2r57	UTSW	7	41,078,225 (GRCm39)	missense	probably benign	0.00
R1025:Vmn2r57	UTSW	7	41,077,228 (GRCm39)	missense	probably benign	0.24
R1081:Vmn2r57	UTSW	7	41,077,635 (GRCm39)	missense	possibly damaging	0.47
R1479:Vmn2r57	UTSW	7	41,077,254 (GRCm39)	missense	possibly damaging	0.45
R1579:Vmn2r57	UTSW	7	41,049,548 (GRCm39)	missense	probably benign	0.38
R1764:Vmn2r57	UTSW	7	41,050,067 (GRCm39)	missense	probably damaging	1.00
R1848:Vmn2r57	UTSW	7	41,077,531 (GRCm39)	missense	probably damaging	1.00
R2006:Vmn2r57	UTSW	7	41,098,001 (GRCm39)	missense	probably benign	0.00
R2197:Vmn2r57	UTSW	7	41,078,249 (GRCm39)	critical splice acceptor site	probably null
R2394:Vmn2r57	UTSW	7	41,049,619 (GRCm39)	missense	possibly damaging	0.73
R3937:Vmn2r57	UTSW	7	41,077,554 (GRCm39)	missense	probably damaging	0.97
R4193:Vmn2r57	UTSW	7	41,077,663 (GRCm39)	missense	probably benign
R4423:Vmn2r57	UTSW	7	41,076,064 (GRCm39)	missense	probably damaging	1.00
R4865:Vmn2r57	UTSW	7	41,049,892 (GRCm39)	missense	probably damaging	1.00
R4947:Vmn2r57	UTSW	7	41,049,919 (GRCm39)	missense	probably damaging	1.00
R5042:Vmn2r57	UTSW	7	41,078,086 (GRCm39)	missense	probably benign	0.06
R5084:Vmn2r57	UTSW	7	41,075,974 (GRCm39)	critical splice donor site	probably null
R5177:Vmn2r57	UTSW	7	41,049,664 (GRCm39)	missense	probably benign	0.31
R5192:Vmn2r57	UTSW	7	41,077,363 (GRCm39)	missense	probably damaging	0.96
R5289:Vmn2r57	UTSW	7	41,049,398 (GRCm39)	missense	probably damaging	0.99
R5745:Vmn2r57	UTSW	7	41,097,895 (GRCm39)	missense	possibly damaging	0.51
R6051:Vmn2r57	UTSW	7	41,097,896 (GRCm39)	missense	probably benign	0.00
R6155:Vmn2r57	UTSW	7	41,078,114 (GRCm39)	missense	probably benign	0.14
R6248:Vmn2r57	UTSW	7	41,049,284 (GRCm39)	missense	probably benign
R6381:Vmn2r57	UTSW	7	41,078,242 (GRCm39)	missense	probably benign	0.08
R7019:Vmn2r57	UTSW	7	41,078,089 (GRCm39)	missense	probably damaging	1.00
R7126:Vmn2r57	UTSW	7	41,049,218 (GRCm39)	missense	possibly damaging	0.93
R7146:Vmn2r57	UTSW	7	41,097,895 (GRCm39)	missense	possibly damaging	0.51
R7215:Vmn2r57	UTSW	7	41,049,710 (GRCm39)	missense	probably benign	0.00
R7432:Vmn2r57	UTSW	7	41,076,148 (GRCm39)	missense	probably benign	0.01
R7633:Vmn2r57	UTSW	7	41,074,513 (GRCm39)	missense	possibly damaging	0.76
R7811:Vmn2r57	UTSW	7	41,074,439 (GRCm39)	nonsense	probably null
R8025:Vmn2r57	UTSW	7	41,076,183 (GRCm39)	missense	probably benign	0.00
R8332:Vmn2r57	UTSW	7	41,049,677 (GRCm39)	missense	probably benign	0.01
R8345:Vmn2r57	UTSW	7	41,076,968 (GRCm39)	missense	possibly damaging	0.81
R8360:Vmn2r57	UTSW	7	41,049,640 (GRCm39)	missense	probably damaging	1.00
R8738:Vmn2r57	UTSW	7	41,077,020 (GRCm39)	missense	probably benign	0.00
R8758:Vmn2r57	UTSW	7	41,078,163 (GRCm39)	missense	probably damaging	1.00
R8955:Vmn2r57	UTSW	7	41,049,571 (GRCm39)	missense	possibly damaging	0.64
R8985:Vmn2r57	UTSW	7	41,049,259 (GRCm39)	missense	probably benign
R9108:Vmn2r57	UTSW	7	41,078,192 (GRCm39)	missense	possibly damaging	0.87
R9160:Vmn2r57	UTSW	7	41,076,159 (GRCm39)	missense	possibly damaging	0.48
R9354:Vmn2r57	UTSW	7	41,049,663 (GRCm39)	missense	probably benign	0.01
R9566:Vmn2r57	UTSW	7	41,077,089 (GRCm39)	missense	probably benign	0.32
R9633:Vmn2r57	UTSW	7	41,076,006 (GRCm39)	missense	probably benign	0.00
X0026:Vmn2r57	UTSW	7	41,077,985 (GRCm39)	missense	possibly damaging	0.91
X0026:Vmn2r57	UTSW	7	41,077,549 (GRCm39)	missense	probably benign	0.03
X0065:Vmn2r57	UTSW	7	41,077,395 (GRCm39)	missense	probably benign	0.09
Z1176:Vmn2r57	UTSW	7	41,049,922 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CTTGTAAAGAAACAGTGTCCCC -3'
(R):5'- ATTGACACTGTGTTTGATGCC -3'

Sequencing Primer
(F):5'- ACAGTGTCCCCATAAATAATAATCAC -3'
(R):5'- GACACTGTGTTTGATGCCAATATTC -3'

Posted On

2014-10-15