Mutagenetix > Incidental Mutation

Incidental Mutation 'R2516:Exosc9'

253753

Institutional Source

Beutler Lab

Gene Symbol

Exosc9

Ensembl Gene

ENSMUSG00000027714

Gene Name

exosome component 9

Synonyms

p5, PM/Scl-75, p6, Pmscl1, RRP45

MMRRC Submission

040420-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2516 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

36606755-36619876 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 36617311 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 355 (K355R)

Ref Sequence

ENSEMBL: ENSMUSP00000029269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029269] [ENSMUST00000029270] [ENSMUST00000147380] [ENSMUST00000155866] [ENSMUST00000196316]

AlphaFold

Q9JHI7

Predicted Effect

probably benign
Transcript: ENSMUST00000029269
AA Change: K355R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000029269
Gene: ENSMUSG00000027714
AA Change: K355R

Domain	Start	End	E-Value	Type
Pfam:RNase_PH	31	163	1.7e-25	PFAM
Pfam:RNase_PH_C	189	255	3.4e-14	PFAM
low complexity region	308	324	N/A	INTRINSIC
low complexity region	348	366	N/A	INTRINSIC
low complexity region	396	406	N/A	INTRINSIC
low complexity region	425	435	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000029270

SMART Domains

Protein: ENSMUSP00000029270
Gene: ENSMUSG00000027715

Domain	Start	End	E-Value	Type
Pfam:Cyclin_N2	22	157	7.2e-50	PFAM
CYCLIN	206	290	1.07e-28	SMART
Cyclin_C	299	417	4.09e-31	SMART
CYCLIN	303	386	1.02e-11	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000131316
AA Change: K53R

SMART Domains

Protein: ENSMUSP00000117235
Gene: ENSMUSG00000027714
AA Change: K53R

Domain	Start	End	E-Value	Type
low complexity region	7	23	N/A	INTRINSIC
low complexity region	47	65	N/A	INTRINSIC
low complexity region	95	105	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133854

Predicted Effect

probably benign
Transcript: ENSMUST00000147380

Predicted Effect

probably benign
Transcript: ENSMUST00000155866

SMART Domains

Protein: ENSMUSP00000122189
Gene: ENSMUSG00000027714

Domain	Start	End	E-Value	Type
Pfam:RNase_PH	31	163	2.6e-25	PFAM
Pfam:RNase_PH_C	189	241	1e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156100

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156575

Predicted Effect

probably benign
Transcript: ENSMUST00000196316

SMART Domains

Protein: ENSMUSP00000142946
Gene: ENSMUSG00000027715

Domain	Start	End	E-Value	Type
CYCLIN	69	153	6.6e-31	SMART
Cyclin_C	162	280	2e-35	SMART
CYCLIN	166	249	6.1e-14	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

98% (65/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a component of the human exosome, a exoribonuclease complex which processes and degrades RNA in the nucleus and cytoplasm. This component may play a role in mRNA degradation and the polymyositis/scleroderma autoantigen complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb11	A	T	2: 69,159,673 (GRCm39)	I6N	possibly damaging	Het
Aen	T	C	7: 78,555,616 (GRCm39)	V188A	probably damaging	Het
Afg3l1	A	G	8: 124,228,693 (GRCm39)	E753G	probably damaging	Het
Alas1	G	A	9: 106,115,859 (GRCm39)	T385I	probably damaging	Het
Alms1	C	A	6: 85,644,945 (GRCm39)		probably benign	Het
Ankrd65	A	G	4: 155,875,868 (GRCm39)	T30A	possibly damaging	Het
App	T	C	16: 84,775,117 (GRCm39)	S582G	probably damaging	Het
Arfgef1	A	T	1: 10,223,879 (GRCm39)	V1473E	possibly damaging	Het
Arhgap21	G	A	2: 20,859,809 (GRCm39)	P1196S	probably damaging	Het
Arhgap24	A	G	5: 103,039,776 (GRCm39)	T238A	probably benign	Het
Atf7	T	C	15: 102,437,439 (GRCm39)		probably benign	Het
Best1	G	T	19: 9,970,675 (GRCm39)	S55*	probably null	Het
Capn11	A	G	17: 45,944,725 (GRCm39)	V514A	probably damaging	Het
Cep104	T	A	4: 154,073,603 (GRCm39)	M52K	probably damaging	Het
Clca3a1	C	T	3: 144,443,619 (GRCm39)		probably null	Het
Cyp3a25	T	C	5: 145,939,837 (GRCm39)		probably null	Het
Dmxl2	G	A	9: 54,307,378 (GRCm39)	P2197S	probably damaging	Het
Drosha	T	C	15: 12,859,551 (GRCm39)		probably null	Het
Fut1	A	C	7: 45,268,622 (GRCm39)	H192P	probably benign	Het
Gm572	T	A	4: 148,748,841 (GRCm39)	V166D	possibly damaging	Het
Gm9966	C	T	7: 95,607,735 (GRCm39)	P19S	unknown	Het
Gmds	C	T	13: 32,284,456 (GRCm39)	V219I	probably damaging	Het
Gsn	G	A	2: 35,173,965 (GRCm39)	E25K	probably benign	Het
Il4i1	T	C	7: 44,489,315 (GRCm39)	F368S	probably damaging	Het
Irak1bp1	T	C	9: 82,712,373 (GRCm39)	L98P	probably damaging	Het
Khdrbs3	T	C	15: 68,896,544 (GRCm39)		probably benign	Het
Kndc1	T	C	7: 139,501,738 (GRCm39)	I925T	probably damaging	Het
Laptm4a	T	C	12: 8,988,151 (GRCm39)	I296T	probably benign	Het
Lpl	A	T	8: 69,340,170 (GRCm39)	H55L	probably benign	Het
Lrrk2	C	A	15: 91,640,130 (GRCm39)	N1558K	probably benign	Het
Mfsd2a	A	G	4: 122,844,280 (GRCm39)	L289P	probably damaging	Het
Mmrn2	T	A	14: 34,120,759 (GRCm39)	M543K	probably benign	Het
Mnat1	T	C	12: 73,228,550 (GRCm39)		probably benign	Het
Msto1	A	T	3: 88,819,200 (GRCm39)		probably null	Het
Mtus1	A	G	8: 41,535,776 (GRCm39)	Y647H	probably damaging	Het
Nars1	A	T	18: 64,638,087 (GRCm39)	V289E	probably damaging	Het
Oit3	T	A	10: 59,264,167 (GRCm39)	K322N	probably damaging	Het
Oit3	G	A	10: 59,277,507 (GRCm39)		probably benign	Het
Or4k1	T	C	14: 50,377,440 (GRCm39)	I219V	probably benign	Het
Or5b113	T	A	19: 13,342,557 (GRCm39)	C188*	probably null	Het
Or5m12	A	G	2: 85,734,900 (GRCm39)	I166T	probably benign	Het
Or6c211	G	A	10: 129,506,155 (GRCm39)	R78W	probably damaging	Het
Pecr	A	T	1: 72,316,469 (GRCm39)	C79S	probably damaging	Het
Plekhn1	T	C	4: 156,307,116 (GRCm39)	D478G	probably damaging	Het
Pls1	A	T	9: 95,658,616 (GRCm39)	M264K	probably benign	Het
Ptprj	C	A	2: 90,305,340 (GRCm39)		probably benign	Het
Pygm	A	G	19: 6,447,631 (GRCm39)	D646G	probably benign	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Scn8a	T	C	15: 100,867,043 (GRCm39)	V283A	probably benign	Het
Shisa5	T	C	9: 108,885,575 (GRCm39)		probably null	Het
Slc10a4	A	G	5: 73,165,848 (GRCm39)	I246V	possibly damaging	Het
Slc1a1	A	T	19: 28,870,312 (GRCm39)	I104F	probably benign	Het
Slc22a8	A	G	19: 8,587,559 (GRCm39)	Y511C	probably benign	Het
Slc6a5	A	G	7: 49,606,210 (GRCm39)	N706S	probably benign	Het
Sos2	T	C	12: 69,697,433 (GRCm39)	K96E	probably damaging	Het
Stom	G	A	2: 35,205,977 (GRCm39)	R251*	probably null	Het
Sycp1	T	C	3: 102,752,382 (GRCm39)	E800G	probably benign	Het
Tab2	G	A	10: 7,783,245 (GRCm39)	P679L	probably damaging	Het
Tiam2	G	A	17: 3,503,657 (GRCm39)	V945I	probably damaging	Het
Trpm5	G	T	7: 142,628,254 (GRCm39)	P1007Q	probably damaging	Het
Uchl1	T	G	5: 66,839,956 (GRCm39)	I139S	probably damaging	Het
Vmn1r232	A	G	17: 21,134,288 (GRCm39)	I104T	possibly damaging	Het
Vmn2r97	G	A	17: 19,167,814 (GRCm39)	M689I	probably benign	Het
Zc3h18	A	T	8: 123,129,904 (GRCm39)		probably benign	Het
Zfhx4	C	T	3: 5,468,418 (GRCm39)	P2859S	probably benign	Het
Zfp456	T	C	13: 67,510,491 (GRCm39)	K99R	probably benign	Het

Other mutations in Exosc9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00419:Exosc9	APN	3	36,607,288 (GRCm39)	unclassified	probably benign
IGL00949:Exosc9	APN	3	36,617,415 (GRCm39)	unclassified	probably benign
IGL01718:Exosc9	APN	3	36,608,078 (GRCm39)	unclassified	probably benign
IGL02072:Exosc9	APN	3	36,608,821 (GRCm39)	missense	probably damaging	1.00
IGL02217:Exosc9	APN	3	36,606,893 (GRCm39)	missense	probably damaging	0.99
IGL02439:Exosc9	APN	3	36,607,180 (GRCm39)	unclassified	probably benign
IGL02871:Exosc9	APN	3	36,619,430 (GRCm39)	missense	probably benign	0.00
IGL02994:Exosc9	APN	3	36,607,287 (GRCm39)	unclassified	probably benign
IGL03144:Exosc9	APN	3	36,608,284 (GRCm39)	missense	probably damaging	1.00
R0909:Exosc9	UTSW	3	36,608,853 (GRCm39)	missense	probably damaging	1.00
R1192:Exosc9	UTSW	3	36,606,904 (GRCm39)	unclassified	probably benign
R4288:Exosc9	UTSW	3	36,617,365 (GRCm39)	missense	probably benign
R4770:Exosc9	UTSW	3	36,607,984 (GRCm39)	missense	probably damaging	0.98
R5875:Exosc9	UTSW	3	36,615,342 (GRCm39)	critical splice donor site	probably null
R5928:Exosc9	UTSW	3	36,609,774 (GRCm39)	intron	probably benign
R6120:Exosc9	UTSW	3	36,608,821 (GRCm39)	missense	probably damaging	1.00
R7077:Exosc9	UTSW	3	36,607,205 (GRCm39)	missense	probably damaging	1.00
R7340:Exosc9	UTSW	3	36,615,297 (GRCm39)	missense	possibly damaging	0.86
R7443:Exosc9	UTSW	3	36,607,990 (GRCm39)	missense	probably damaging	1.00
R7917:Exosc9	UTSW	3	36,607,968 (GRCm39)	missense	probably damaging	0.99
R8735:Exosc9	UTSW	3	36,609,662 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAGTCTTCCAGGAAATCTGATC -3'
(R):5'- CTAAACAAGGCTGTTGGCAGG -3'

Sequencing Primer
(F):5'- AGGAAATCTGATCTCTTCTTGGTAGC -3'
(R):5'- TCTCCACAGTGCTGGGATCAAAG -3'

Posted On

2014-12-04