Incidental Mutation 'R3118:Rgs10'
ID 263127
Institutional Source Beutler Lab
Gene Symbol Rgs10
Ensembl Gene ENSMUSG00000030844
Gene Name regulator of G-protein signalling 10
Synonyms 2310010N19Rik
MMRRC Submission 040591-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.271) question?
Stock # R3118 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 127975345-128020482 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 128004955 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Glycine at position 65 (E65G)
Ref Sequence ENSEMBL: ENSMUSP00000146128 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033133] [ENSMUST00000145739] [ENSMUST00000147840]
AlphaFold Q9CQE5
Predicted Effect probably benign
Transcript: ENSMUST00000033133
AA Change: E65G

PolyPhen 2 Score 0.259 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000033133
Gene: ENSMUSG00000030844
AA Change: E65G

DomainStartEndE-ValueType
low complexity region 16 31 N/A INTRINSIC
RGS 41 156 7.05e-46 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129120
Predicted Effect probably damaging
Transcript: ENSMUST00000145739
AA Change: E65G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
Predicted Effect probably damaging
Transcript: ENSMUST00000147840
AA Change: E69G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000122030
Gene: ENSMUSG00000030844
AA Change: E69G

DomainStartEndE-ValueType
low complexity region 24 32 N/A INTRINSIC
Pfam:RGS 45 85 1.5e-15 PFAM
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: A gene trap mutation of this gene results in impaired glucose tolerance and increased fasting glucose levels whereas a targeted knockout shows defects in osteoclast differentiation and in the skeleton. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 28 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adamts6 T G 13: 104,450,787 (GRCm39) D323E possibly damaging Het
Ccdc125 T C 13: 100,826,827 (GRCm39) V228A possibly damaging Het
Chrna2 A G 14: 66,388,442 (GRCm39) I486V probably damaging Het
Cpxm1 T C 2: 130,235,493 (GRCm39) T500A possibly damaging Het
Crebbp G A 16: 3,927,062 (GRCm39) R628C probably damaging Het
Cxcl1 T A 5: 91,039,454 (GRCm39) probably null Het
Dab1 G A 4: 104,537,266 (GRCm39) probably null Het
Ddx11 T A 17: 66,456,272 (GRCm39) M751K probably damaging Het
Ece1 A G 4: 137,675,855 (GRCm39) T410A probably benign Het
Eml5 C T 12: 98,831,753 (GRCm39) V402I probably damaging Het
Fam241b A T 10: 61,944,635 (GRCm39) *121R probably null Het
Fras1 G A 5: 96,919,571 (GRCm39) A3595T probably damaging Het
Gpr149 A G 3: 62,502,443 (GRCm39) V471A probably benign Het
Lemd3 A G 10: 120,783,156 (GRCm39) S557P probably benign Het
Mkrn3 CGGCATTGGCACTGGCATTGGCACTGGCATTGGCA CGGCATTGGCACTGGCATTGGCA 7: 62,068,962 (GRCm39) probably benign Het
Mmp7 T C 9: 7,697,693 (GRCm39) Y243H probably benign Het
Obscn T C 11: 59,022,472 (GRCm39) R758G possibly damaging Het
Pak6 T C 2: 118,520,222 (GRCm39) V71A probably damaging Het
Pira2 T A 7: 3,844,676 (GRCm39) R452* probably null Het
Plxna1 A G 6: 89,333,958 (GRCm39) S224P possibly damaging Het
Prpf39 T C 12: 65,104,651 (GRCm39) V572A possibly damaging Het
Prss12 A T 3: 123,298,976 (GRCm39) T583S possibly damaging Het
Rnf19a T A 15: 36,242,045 (GRCm39) K665* probably null Het
Rufy4 T C 1: 74,186,822 (GRCm39) C537R probably damaging Het
Tbx15 T C 3: 99,259,470 (GRCm39) I447T probably damaging Het
Tmem135 A G 7: 88,797,005 (GRCm39) S364P probably benign Het
Ugt1a9 T C 1: 87,998,562 (GRCm39) V4A probably benign Het
Zfp595 T C 13: 67,468,963 (GRCm39) I95M probably benign Het
Other mutations in Rgs10
AlleleSourceChrCoordTypePredicted EffectPPH Score
comptroller UTSW 7 127,975,733 (GRCm39) missense probably damaging 1.00
R1682:Rgs10 UTSW 7 127,975,694 (GRCm39) missense probably benign
R1714:Rgs10 UTSW 7 128,004,946 (GRCm39) missense probably damaging 0.98
R1801:Rgs10 UTSW 7 128,006,201 (GRCm39) missense possibly damaging 0.67
R2200:Rgs10 UTSW 7 127,990,761 (GRCm39) missense probably damaging 0.99
R3119:Rgs10 UTSW 7 128,004,955 (GRCm39) missense probably damaging 1.00
R6903:Rgs10 UTSW 7 127,990,797 (GRCm39) missense probably damaging 1.00
R8504:Rgs10 UTSW 7 128,019,793 (GRCm39) missense probably benign 0.38
R8834:Rgs10 UTSW 7 127,990,809 (GRCm39) missense probably damaging 1.00
R9153:Rgs10 UTSW 7 127,975,733 (GRCm39) missense probably damaging 1.00
R9709:Rgs10 UTSW 7 127,975,729 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TGGTACCAGTGAGAATGATCTG -3'
(R):5'- TGTGTCTTGCAATGTCAAAACC -3'

Sequencing Primer
(F):5'- CCAGTGAGAATGATCTGGGCAC -3'
(R):5'- GTCTTGCAATGTCAAAACCATTAAGG -3'
Posted On 2015-02-05