Mutagenetix > Incidental Mutation

Incidental Mutation 'R3427:Vmn2r63'

267915

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

040645-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R3427 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

42552675-42583213 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42576406 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 469 (F469S)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

probably benign
Transcript: ENSMUST00000163803
AA Change: F469S

PolyPhen 2 Score 0.040 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: F469S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actl11	T	A	9: 107,806,969 (GRCm39)	F431I	probably damaging	Het
Asb18	C	T	1: 89,896,315 (GRCm39)	G242S	probably damaging	Het
Atp13a3	T	C	16: 30,163,411 (GRCm39)	I582V	probably benign	Het
BC034090	T	C	1: 155,117,244 (GRCm39)	I291M	probably benign	Het
Bsph1	T	C	7: 13,206,168 (GRCm39)	Y78H	probably damaging	Het
Chd6	G	T	2: 160,832,175 (GRCm39)	T999N	probably damaging	Het
Cps1	T	A	1: 67,213,653 (GRCm39)	V795E	probably damaging	Het
Crhr1	A	G	11: 104,064,419 (GRCm39)		probably null	Het
Crygs	C	T	16: 22,624,301 (GRCm39)	G102D	possibly damaging	Het
Ctbp2	A	T	7: 132,593,321 (GRCm39)	H793Q	probably damaging	Het
Cul5	T	C	9: 53,529,190 (GRCm39)	M805V	probably benign	Het
Dctn3	T	C	4: 41,719,858 (GRCm39)	K83E	probably damaging	Het
Ep300	A	G	15: 81,485,480 (GRCm39)	N156D	unknown	Het
Epb42	A	T	2: 120,860,520 (GRCm39)	L160M	probably damaging	Het
Exosc2	T	C	2: 31,569,900 (GRCm39)	L237P	probably damaging	Het
Fiz1	A	G	7: 5,015,708 (GRCm39)	F94S	probably damaging	Het
Gm5422	A	G	10: 31,124,842 (GRCm39)		noncoding transcript	Het
Igsf9b	T	C	9: 27,245,873 (GRCm39)	F1280S	probably damaging	Het
Igtp	G	A	11: 58,097,419 (GRCm39)	V197I	probably damaging	Het
Kcnh1	T	C	1: 191,924,238 (GRCm39)	F151L	probably benign	Het
Nudc	C	T	4: 133,261,568 (GRCm39)	G239S	probably benign	Het
Or6c215	T	C	10: 129,637,720 (GRCm39)	K225E	possibly damaging	Het
Plekhb1	T	C	7: 100,294,857 (GRCm39)	Y172C	probably damaging	Het
Plscr4	T	C	9: 92,370,797 (GRCm39)	S255P	probably damaging	Het
Rbm26	A	G	14: 105,368,968 (GRCm39)	V737A	probably damaging	Het
Rps6ka4	C	A	19: 6,815,123 (GRCm39)		probably null	Het
Rsf1	GCG	GCGACGGCGTCG	7: 97,229,114 (GRCm39)		probably benign	Het
Rttn	G	A	18: 89,113,775 (GRCm39)		probably null	Het
Slc22a5	A	G	11: 53,760,152 (GRCm39)	V388A	probably benign	Het
Strip1	T	C	3: 107,524,138 (GRCm39)	H593R	possibly damaging	Het
Sycp1	A	T	3: 102,783,666 (GRCm39)	C603S	probably benign	Het
Tmem181a	T	A	17: 6,346,061 (GRCm39)	L185H	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Trpc1	C	T	9: 95,614,249 (GRCm39)	R5Q	probably benign	Het
Trpv3	A	T	11: 73,176,767 (GRCm39)	Y382F	probably damaging	Het
Unc80	G	A	1: 66,678,464 (GRCm39)	V2082I	probably benign	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42,553,543 (GRCm39)	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42,552,788 (GRCm39)	missense	probably benign
IGL02203:Vmn2r63	APN	7	42,553,432 (GRCm39)	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42,576,274 (GRCm39)	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42,552,878 (GRCm39)	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42,578,616 (GRCm39)	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42,577,368 (GRCm39)	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42,553,409 (GRCm39)	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42,576,514 (GRCm39)	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42,552,699 (GRCm39)	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42,553,042 (GRCm39)	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42,583,129 (GRCm39)	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42,577,952 (GRCm39)	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42,577,434 (GRCm39)	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42,577,459 (GRCm39)	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42,577,339 (GRCm39)	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42,578,015 (GRCm39)	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42,553,550 (GRCm39)	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42,577,743 (GRCm39)	missense	probably benign
R1698:Vmn2r63	UTSW	7	42,583,038 (GRCm39)	missense	probably benign
R1753:Vmn2r63	UTSW	7	42,577,669 (GRCm39)	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42,576,297 (GRCm39)	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42,583,004 (GRCm39)	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42,552,829 (GRCm39)	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42,577,537 (GRCm39)	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42,583,250 (GRCm39)	splice site	probably null
R4649:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42,576,314 (GRCm39)	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42,553,402 (GRCm39)	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42,553,169 (GRCm39)	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42,577,701 (GRCm39)	missense	probably benign
R5400:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42,583,104 (GRCm39)	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42,578,435 (GRCm39)	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42,578,059 (GRCm39)	splice site	probably null
R6362:Vmn2r63	UTSW	7	42,552,721 (GRCm39)	missense	probably benign
R6706:Vmn2r63	UTSW	7	42,578,001 (GRCm39)	missense	probably damaging	1.00
R6764:Vmn2r63	UTSW	7	42,552,695 (GRCm39)	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42,577,959 (GRCm39)	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42,583,014 (GRCm39)	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42,576,391 (GRCm39)	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42,574,693 (GRCm39)	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42,576,466 (GRCm39)	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42,577,553 (GRCm39)	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42,552,955 (GRCm39)	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42,576,441 (GRCm39)	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42,577,919 (GRCm39)	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42,577,874 (GRCm39)	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42,576,413 (GRCm39)	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42,553,361 (GRCm39)	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42,577,983 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- GAGTGTCCAATTAACACCATATCC -3'
(R):5'- TTCATCATGAGGGGATAAAAGACAC -3'

Sequencing Primer
(F):5'- GTGTCGGCCATTGTATCA -3'
(R):5'- GGGATAAAAGACACTGAGATTGTTTG -3'

Posted On

2015-02-18