Mutagenetix > Incidental Mutation

Incidental Mutation 'R6811:Atrnl1'

533829

Institutional Source

Beutler Lab

Gene Symbol

Atrnl1

Ensembl Gene

ENSMUSG00000054843

Gene Name

attractin like 1

Synonyms

Alp

MMRRC Submission

045018-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.182) question?

Stock #

R6811 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

57599466-58121775 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 57643393 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Arginine at position 427 (M427R)

Ref Sequence

ENSEMBL: ENSMUSP00000076514 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077282]

AlphaFold

Q6A051

Predicted Effect

probably damaging
Transcript: ENSMUST00000077282
AA Change: M427R

PolyPhen 2 Score 0.983 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000076514
Gene: ENSMUSG00000054843
AA Change: M427R

Domain	Start	End	E-Value	Type
low complexity region	25	32	N/A	INTRINSIC
EGF	61	90	5.71e-1	SMART
CUB	92	208	1.43e-11	SMART
EGF	209	244	1.95e1	SMART
Pfam:EGF_2	248	279	5.8e-7	PFAM
Pfam:Kelch_5	350	391	2.1e-9	PFAM
Pfam:Kelch_6	354	401	5.8e-8	PFAM
Pfam:Kelch_4	465	517	4.3e-7	PFAM
Pfam:Kelch_1	519	573	2.7e-6	PFAM
PSI	613	656	3.38e-1	SMART
PSI	665	708	2e-3	SMART
PSI	714	759	1.72e-2	SMART
CLECT	747	872	2.86e-20	SMART
PSI	888	938	6.26e-5	SMART
PSI	941	1011	1.73e-7	SMART
EGF_Lam	1013	1056	1.07e-5	SMART
low complexity region	1157	1173	N/A	INTRINSIC
transmembrane domain	1229	1251	N/A	INTRINSIC
low complexity region	1261	1272	N/A	INTRINSIC
low complexity region	1326	1339	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 99.1%
20x: 97.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit normal coat coloring and normal brain morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb1	T	C	15: 74,401,210 (GRCm39)	S69P	probably damaging	Het
Alkbh7	G	T	17: 57,304,392 (GRCm39)	R10L	probably benign	Het
Ano3	T	C	2: 110,711,212 (GRCm39)	E84G	probably benign	Het
Asxl3	A	G	18: 22,655,968 (GRCm39)	E1326G	possibly damaging	Het
Cenpf	T	C	1: 189,386,739 (GRCm39)	E1847G	probably benign	Het
Csf1r	T	C	18: 61,252,125 (GRCm39)	Y536H	probably damaging	Het
Dnm3	T	C	1: 162,148,652 (GRCm39)	K240E	probably damaging	Het
Dsc1	T	G	18: 20,222,711 (GRCm39)	E587A	probably benign	Het
Gm19410	C	A	8: 36,239,733 (GRCm39)	A143E	probably damaging	Het
Gm19965	G	T	1: 116,731,809 (GRCm39)	L38F	probably damaging	Het
Helz	T	C	11: 107,510,144 (GRCm39)		probably null	Het
Herc2	C	T	7: 55,763,181 (GRCm39)	R929*	probably null	Het
Iqcn	T	C	8: 71,169,422 (GRCm39)	S1171P	probably benign	Het
Krt31	A	G	11: 99,939,242 (GRCm39)	L225P	probably damaging	Het
Lrp1b	T	C	2: 40,605,512 (GRCm39)		probably null	Het
Lrp1b	A	G	2: 41,339,206 (GRCm39)	V765A	probably benign	Het
Lsm5	A	G	6: 56,679,127 (GRCm39)	I34T	possibly damaging	Het
Ly6g6c	T	C	17: 35,288,386 (GRCm39)	L86P	probably damaging	Het
Meak7	A	T	8: 120,495,029 (GRCm39)	I243N	possibly damaging	Het
Megf11	A	G	9: 64,451,923 (GRCm39)	T116A	probably damaging	Het
Megf6	T	C	4: 154,336,618 (GRCm39)	C190R	probably damaging	Het
Mroh9	A	G	1: 162,873,610 (GRCm39)	V515A	possibly damaging	Het
Mtbp	C	A	15: 55,469,942 (GRCm39)		probably null	Het
Myo9b	T	A	8: 71,809,222 (GRCm39)	F1810L	probably damaging	Het
Nacad	T	G	11: 6,549,400 (GRCm39)	K180Q	possibly damaging	Het
Ncf2	G	A	1: 152,711,791 (GRCm39)	V502I	probably benign	Het
Npsr1	T	A	9: 24,046,105 (GRCm39)	C75S	probably benign	Het
Oog3	T	C	4: 143,886,152 (GRCm39)	T149A	probably benign	Het
Pank1	T	A	19: 34,818,422 (GRCm39)	Q39L	probably benign	Het
Pdx1	T	C	5: 147,211,474 (GRCm39)	S232P	possibly damaging	Het
Peg10	T	TCCA	6: 4,756,451 (GRCm39)		probably benign	Het
Pirb	A	T	7: 3,722,641 (GRCm39)	V117E	possibly damaging	Het
Ppl	G	A	16: 4,907,008 (GRCm39)	L1096F	probably damaging	Het
Rev3l	T	A	10: 39,706,917 (GRCm39)	Y2223*	probably null	Het
Slc16a4	A	G	3: 107,206,233 (GRCm39)	Y101C	probably benign	Het
Slc17a3	A	T	13: 24,039,924 (GRCm39)	I321F	possibly damaging	Het
Sufu	T	A	19: 46,438,317 (GRCm39)	D168E	probably damaging	Het
Tenm4	G	A	7: 96,202,703 (GRCm39)	R106H	probably benign	Het
Vmn1r237	T	G	17: 21,534,648 (GRCm39)	S124A	probably benign	Het
Vmn2r60	T	A	7: 41,844,310 (GRCm39)	C558S	probably damaging	Het
Vwa5b1	G	A	4: 138,319,414 (GRCm39)	T414I	probably benign	Het
Zbtb47	T	C	9: 121,595,595 (GRCm39)	S573P	probably damaging	Het
Zfp971	T	G	2: 177,675,674 (GRCm39)	C424W	possibly damaging	Het

Other mutations in Atrnl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Atrnl1	APN	19	57,680,249 (GRCm39)	missense	probably benign	0.02
IGL00707:Atrnl1	APN	19	57,661,697 (GRCm39)	missense	probably damaging	0.96
IGL00921:Atrnl1	APN	19	57,690,585 (GRCm39)	missense	probably damaging	1.00
IGL01410:Atrnl1	APN	19	58,119,536 (GRCm39)	missense	probably damaging	1.00
IGL01468:Atrnl1	APN	19	57,688,144 (GRCm39)	missense	probably benign	0.02
IGL01756:Atrnl1	APN	19	57,641,380 (GRCm39)	missense	probably benign
IGL01971:Atrnl1	APN	19	57,741,715 (GRCm39)	missense	probably damaging	1.00
IGL02019:Atrnl1	APN	19	57,680,195 (GRCm39)	splice site	probably benign
IGL02580:Atrnl1	APN	19	57,703,008 (GRCm39)	splice site	probably benign
IGL02649:Atrnl1	APN	19	57,638,873 (GRCm39)	splice site	probably benign
IGL02676:Atrnl1	APN	19	57,680,316 (GRCm39)	missense	probably damaging	1.00
IGL03276:Atrnl1	APN	19	57,641,359 (GRCm39)	missense	probably damaging	0.99
IGL03379:Atrnl1	APN	19	57,630,973 (GRCm39)	missense	probably benign	0.02
Magnetogorsk	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
polar	UTSW	19	57,641,382 (GRCm39)	missense	probably benign	0.00
PIT4812001:Atrnl1	UTSW	19	57,720,055 (GRCm39)	missense	probably benign	0.08
R0109:Atrnl1	UTSW	19	57,743,949 (GRCm39)	missense	possibly damaging	0.78
R0308:Atrnl1	UTSW	19	57,741,720 (GRCm39)	missense	probably benign	0.04
R0394:Atrnl1	UTSW	19	57,661,608 (GRCm39)	missense	probably benign	0.10
R0734:Atrnl1	UTSW	19	57,643,293 (GRCm39)	missense	probably damaging	1.00
R0811:Atrnl1	UTSW	19	57,661,573 (GRCm39)	missense	probably benign	0.07
R0812:Atrnl1	UTSW	19	57,661,573 (GRCm39)	missense	probably benign	0.07
R1183:Atrnl1	UTSW	19	57,638,725 (GRCm39)	missense	probably damaging	0.97
R1213:Atrnl1	UTSW	19	57,626,894 (GRCm39)	missense	probably benign	0.25
R1344:Atrnl1	UTSW	19	57,924,137 (GRCm39)	critical splice donor site	probably null
R1418:Atrnl1	UTSW	19	57,924,137 (GRCm39)	critical splice donor site	probably null
R1707:Atrnl1	UTSW	19	57,675,169 (GRCm39)	missense	probably benign	0.00
R1748:Atrnl1	UTSW	19	57,703,134 (GRCm39)	missense	probably damaging	0.99
R2051:Atrnl1	UTSW	19	57,680,281 (GRCm39)	missense	probably benign	0.01
R2113:Atrnl1	UTSW	19	57,744,048 (GRCm39)	nonsense	probably null
R2130:Atrnl1	UTSW	19	57,643,426 (GRCm39)	missense	probably damaging	1.00
R3710:Atrnl1	UTSW	19	57,645,546 (GRCm39)	missense	probably damaging	1.00
R3916:Atrnl1	UTSW	19	57,924,084 (GRCm39)	missense	possibly damaging	0.82
R4524:Atrnl1	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
R4707:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4712:Atrnl1	UTSW	19	57,641,382 (GRCm39)	missense	probably benign	0.00
R4784:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4785:Atrnl1	UTSW	19	57,617,590 (GRCm39)	missense	probably damaging	0.97
R4798:Atrnl1	UTSW	19	58,030,793 (GRCm39)	missense	probably benign
R5172:Atrnl1	UTSW	19	57,673,945 (GRCm39)	nonsense	probably null
R5226:Atrnl1	UTSW	19	57,638,767 (GRCm39)	missense	probably benign
R5289:Atrnl1	UTSW	19	57,645,514 (GRCm39)	missense	probably damaging	1.00
R5372:Atrnl1	UTSW	19	57,743,968 (GRCm39)	missense	probably benign
R5737:Atrnl1	UTSW	19	57,766,320 (GRCm39)	missense	possibly damaging	0.84
R5782:Atrnl1	UTSW	19	57,741,718 (GRCm39)	missense	possibly damaging	0.95
R5826:Atrnl1	UTSW	19	57,618,724 (GRCm39)	nonsense	probably null
R6169:Atrnl1	UTSW	19	57,630,895 (GRCm39)	missense	probably benign	0.00
R6242:Atrnl1	UTSW	19	57,630,910 (GRCm39)	missense	probably benign	0.02
R6342:Atrnl1	UTSW	19	57,626,942 (GRCm39)	missense	probably damaging	1.00
R6372:Atrnl1	UTSW	19	57,638,764 (GRCm39)	missense	probably benign	0.01
R6897:Atrnl1	UTSW	19	58,030,800 (GRCm39)	missense	probably benign	0.01
R7024:Atrnl1	UTSW	19	57,626,882 (GRCm39)	critical splice acceptor site	probably null
R7085:Atrnl1	UTSW	19	57,680,289 (GRCm39)	missense	probably damaging	1.00
R7144:Atrnl1	UTSW	19	58,030,784 (GRCm39)	missense	probably damaging	1.00
R7259:Atrnl1	UTSW	19	57,924,038 (GRCm39)	nonsense	probably null
R7289:Atrnl1	UTSW	19	57,638,846 (GRCm39)	missense	probably benign	0.13
R7310:Atrnl1	UTSW	19	57,630,856 (GRCm39)	missense	possibly damaging	0.69
R7372:Atrnl1	UTSW	19	57,924,078 (GRCm39)	missense	possibly damaging	0.47
R7432:Atrnl1	UTSW	19	57,743,956 (GRCm39)	missense	probably damaging	1.00
R7478:Atrnl1	UTSW	19	57,684,744 (GRCm39)	missense	possibly damaging	0.89
R7556:Atrnl1	UTSW	19	57,643,278 (GRCm39)	missense	probably benign
R7567:Atrnl1	UTSW	19	57,687,955 (GRCm39)	missense	probably damaging	0.98
R7608:Atrnl1	UTSW	19	57,703,119 (GRCm39)	missense	probably damaging	1.00
R7632:Atrnl1	UTSW	19	57,618,738 (GRCm39)	missense	probably damaging	1.00
R7655:Atrnl1	UTSW	19	57,599,811 (GRCm39)	nonsense	probably null
R7656:Atrnl1	UTSW	19	57,599,811 (GRCm39)	nonsense	probably null
R7718:Atrnl1	UTSW	19	57,728,615 (GRCm39)	nonsense	probably null
R7721:Atrnl1	UTSW	19	57,684,763 (GRCm39)	missense	probably benign	0.00
R7726:Atrnl1	UTSW	19	57,690,504 (GRCm39)	missense	probably damaging	1.00
R7733:Atrnl1	UTSW	19	57,690,420 (GRCm39)	missense	probably benign	0.00
R7774:Atrnl1	UTSW	19	57,688,103 (GRCm39)	missense	probably damaging	1.00
R8010:Atrnl1	UTSW	19	57,670,878 (GRCm39)	missense	probably benign	0.14
R8119:Atrnl1	UTSW	19	57,630,895 (GRCm39)	missense	probably benign	0.00
R9242:Atrnl1	UTSW	19	57,645,660 (GRCm39)	missense	probably benign	0.07
R9265:Atrnl1	UTSW	19	57,766,359 (GRCm39)	missense	probably benign	0.11
R9272:Atrnl1	UTSW	19	57,643,420 (GRCm39)	missense	probably benign	0.00
R9480:Atrnl1	UTSW	19	57,690,420 (GRCm39)	missense	possibly damaging	0.61
R9526:Atrnl1	UTSW	19	57,617,551 (GRCm39)	missense	probably damaging	0.99
R9672:Atrnl1	UTSW	19	57,618,695 (GRCm39)	missense	possibly damaging	0.87
R9673:Atrnl1	UTSW	19	57,599,786 (GRCm39)	start codon destroyed	probably null	0.04
RF021:Atrnl1	UTSW	19	57,630,905 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTTTATGTACGGAGGCAGAATGG -3'
(R):5'- TGGCTGAGAGATGATACCCAAG -3'

Sequencing Primer
(F):5'- TGGAGACCAGTGATGGCAAC -3'
(R):5'- GCTGAGAGATGATACCCAAGTATTC -3'

Posted On

2018-09-12