Mutagenetix > Incidental Mutation

Incidental Mutation 'R6901:Zfp936'

538529

Institutional Source

Beutler Lab

Gene Symbol

Zfp936

Ensembl Gene

ENSMUSG00000064194

Gene Name

zinc finger protein 936

Synonyms

EG435970, Gm9272, I1C0022H11Rik

MMRRC Submission

044995-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.686) question?

Stock #

R6901 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

42763653-42841533 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 42839467 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 311 (H311Q)

Ref Sequence

ENSEMBL: ENSMUSP00000143800 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072829] [ENSMUST00000200973] [ENSMUST00000202535] [ENSMUST00000205912]

AlphaFold

Q3ULA8

Predicted Effect

probably damaging
Transcript: ENSMUST00000072829
AA Change: H310Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000072608
Gene: ENSMUSG00000064194
AA Change: H310Q

Domain	Start	End	E-Value	Type
KRAB	3	65	5.32e-19	SMART
ZnF_C2H2	148	170	5.9e-3	SMART
ZnF_C2H2	176	198	5.9e-3	SMART
ZnF_C2H2	204	226	1.4e-4	SMART
ZnF_C2H2	232	254	1.2e-3	SMART
ZnF_C2H2	260	282	7.37e-4	SMART
ZnF_C2H2	288	310	2.27e-4	SMART
ZnF_C2H2	316	338	2.09e-3	SMART
ZnF_C2H2	344	366	1.45e-2	SMART
ZnF_C2H2	372	394	1.82e-3	SMART
ZnF_C2H2	400	422	2.53e-2	SMART
ZnF_C2H2	428	450	4.54e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000200973

SMART Domains

Protein: ENSMUSP00000144191
Gene: ENSMUSG00000064194

Domain	Start	End	E-Value	Type
KRAB	4	66	1.4e-21	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000202535
AA Change: H311Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000143800
Gene: ENSMUSG00000064194
AA Change: H311Q

Domain	Start	End	E-Value	Type
KRAB	4	66	2.3e-21	SMART
ZnF_C2H2	149	171	2.6e-5	SMART
ZnF_C2H2	177	199	2.5e-5	SMART
ZnF_C2H2	205	227	5.9e-7	SMART
ZnF_C2H2	233	255	5.1e-6	SMART
ZnF_C2H2	261	283	3.1e-6	SMART
ZnF_C2H2	289	311	9.4e-7	SMART
ZnF_C2H2	317	339	8.7e-6	SMART
ZnF_C2H2	345	367	6.3e-5	SMART
ZnF_C2H2	373	395	7.7e-6	SMART
ZnF_C2H2	401	423	1.1e-4	SMART
ZnF_C2H2	429	451	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205912

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd5	T	A	9: 122,197,220 (GRCm39)	S136T	probably benign	Het
Anxa10	T	C	8: 62,549,816 (GRCm39)	T10A	probably damaging	Het
Ap3b1	A	G	13: 94,554,650 (GRCm39)	E262G	probably benign	Het
Ap3b2	T	C	7: 81,134,660 (GRCm39)		probably null	Het
Arhgef40	A	G	14: 52,234,825 (GRCm39)	T1043A	probably damaging	Het
Arhgef5	T	A	6: 43,250,232 (GRCm39)	S328T	probably benign	Het
Arid4a	A	G	12: 71,113,911 (GRCm39)	K166R	probably damaging	Het
Cdh12	A	G	15: 21,583,872 (GRCm39)	I571V	probably benign	Het
Cdh4	G	A	2: 179,501,987 (GRCm39)	V316I	probably benign	Het
Cebpg	A	T	7: 34,750,202 (GRCm39)	H20Q	probably benign	Het
Ces4a	G	A	8: 105,873,330 (GRCm39)	V392I	probably benign	Het
CN725425	C	A	15: 91,124,966 (GRCm39)	P169T	possibly damaging	Het
Cyp2c54	A	G	19: 40,058,703 (GRCm39)	F243S	probably damaging	Het
Dkk2	T	A	3: 131,880,887 (GRCm39)		probably null	Het
Dync2h1	A	G	9: 7,131,855 (GRCm39)	Y1651H	probably damaging	Het
Dyrk1b	T	A	7: 27,884,542 (GRCm39)	L346Q	probably damaging	Het
Ephx4	T	C	5: 107,561,427 (GRCm39)	V85A	probably benign	Het
Etv6	A	G	6: 134,243,421 (GRCm39)	E392G	probably benign	Het
Fli1	T	A	9: 32,341,221 (GRCm39)	N202I	probably benign	Het
Gpc6	G	T	14: 118,188,629 (GRCm39)	R421L	possibly damaging	Het
Gse1	A	G	8: 120,956,561 (GRCm39)		probably benign	Het
Hdac1-ps	A	G	17: 78,800,089 (GRCm39)	E360G	probably benign	Het
Hnrnpab	T	C	11: 51,492,675 (GRCm39)		probably benign	Het
Hyal4	A	G	6: 24,756,190 (GRCm39)	E136G	probably damaging	Het
Ift56	G	A	6: 38,378,079 (GRCm39)	R297H	possibly damaging	Het
Kcna3	C	T	3: 106,943,884 (GRCm39)	A49V	probably benign	Het
Krt42	C	T	11: 100,160,542 (GRCm39)	M52I	probably benign	Het
Lyrm4	A	T	13: 36,301,107 (GRCm39)	Y13N	probably damaging	Het
Map2	C	T	1: 66,460,932 (GRCm39)	S1576L	possibly damaging	Het
Map3k12	G	T	15: 102,409,064 (GRCm39)	P817Q	possibly damaging	Het
Map3k12	G	T	15: 102,409,065 (GRCm39)	P817T	possibly damaging	Het
Masp1	T	C	16: 23,332,584 (GRCm39)	K84E	probably damaging	Het
Mki67	A	T	7: 135,310,489 (GRCm39)		probably null	Het
Mrap	C	G	16: 90,546,193 (GRCm39)	S94C	probably damaging	Het
Mybpc2	C	A	7: 44,154,779 (GRCm39)	C986F	probably damaging	Het
Nbea	A	C	3: 55,926,836 (GRCm39)	M789R	probably damaging	Het
Or2ag1b	T	C	7: 106,288,396 (GRCm39)	I181V	probably benign	Het
Or6b2	G	T	1: 92,408,327 (GRCm39)	N5K	probably damaging	Het
Pde4a	A	G	9: 21,116,266 (GRCm39)	N475S	probably benign	Het
Pex7	C	T	10: 19,736,740 (GRCm39)	V297I	probably benign	Het
Pkd1l3	A	T	8: 110,341,246 (GRCm39)	H33L	unknown	Het
Prl8a6	A	T	13: 27,621,030 (GRCm39)	Y67N	possibly damaging	Het
Rcor1	A	T	12: 111,075,322 (GRCm39)	E383V	probably damaging	Het
Slc22a27	A	T	19: 7,903,944 (GRCm39)	S64R	probably damaging	Het
Snx7	G	A	3: 117,623,285 (GRCm39)	Q292*	probably null	Het
Tgm3	C	T	2: 129,883,890 (GRCm39)	T516M	possibly damaging	Het
Trim9	T	C	12: 70,393,413 (GRCm39)	E177G	probably damaging	Het
Ttc28	C	A	5: 111,424,891 (GRCm39)	T1541N	possibly damaging	Het
Ttc8	T	A	12: 98,927,735 (GRCm39)	L202H	probably damaging	Het
Ttll13	T	C	7: 79,899,930 (GRCm39)	Y90H	probably damaging	Het
Uba1y	T	G	Y: 825,496 (GRCm39)	I286S	probably benign	Het
Ubxn11	G	A	4: 133,853,575 (GRCm39)	A125T	probably damaging	Het
Vmn1r48	A	G	6: 90,013,550 (GRCm39)	Y92H	possibly damaging	Het
Vmn1r74	T	C	7: 11,581,368 (GRCm39)	F223L	probably benign	Het
Vwa5b1	G	A	4: 138,313,880 (GRCm39)	T696I	probably benign	Het
Zfp266	G	T	9: 20,410,895 (GRCm39)	Y427*	probably null	Het
Zfp362	C	T	4: 128,679,808 (GRCm39)	C273Y	probably damaging	Het
Zfp385c	T	C	11: 100,523,585 (GRCm39)	N123S	probably benign	Het

Other mutations in Zfp936

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02231:Zfp936	APN	7	42,836,909 (GRCm39)	splice site	probably null
IGL02245:Zfp936	APN	7	42,836,722 (GRCm39)	critical splice donor site	probably null
IGL02335:Zfp936	APN	7	42,836,691 (GRCm39)	missense	probably damaging	1.00
FR4340:Zfp936	UTSW	7	42,838,913 (GRCm39)	missense	possibly damaging	0.63
R0437:Zfp936	UTSW	7	42,838,734 (GRCm39)	missense	probably benign	0.00
R3899:Zfp936	UTSW	7	42,839,158 (GRCm39)	missense	possibly damaging	0.93
R4120:Zfp936	UTSW	7	42,839,630 (GRCm39)	missense	probably benign	0.10
R4406:Zfp936	UTSW	7	42,839,748 (GRCm39)	missense	possibly damaging	0.82
R4959:Zfp936	UTSW	7	42,839,034 (GRCm39)	missense	probably damaging	1.00
R5023:Zfp936	UTSW	7	42,836,681 (GRCm39)	missense	probably damaging	1.00
R5163:Zfp936	UTSW	7	42,839,664 (GRCm39)	missense	probably damaging	1.00
R5182:Zfp936	UTSW	7	42,839,331 (GRCm39)	missense	probably damaging	1.00
R5292:Zfp936	UTSW	7	42,838,759 (GRCm39)	nonsense	probably null
R5668:Zfp936	UTSW	7	42,839,858 (GRCm39)	missense	possibly damaging	0.93
R6057:Zfp936	UTSW	7	42,839,787 (GRCm39)	missense	probably benign	0.00
R7139:Zfp936	UTSW	7	42,839,715 (GRCm39)	missense	possibly damaging	0.54
R7258:Zfp936	UTSW	7	42,839,803 (GRCm39)	missense	probably damaging	1.00
R7440:Zfp936	UTSW	7	42,836,685 (GRCm39)	missense	probably damaging	1.00
R7537:Zfp936	UTSW	7	42,839,239 (GRCm39)	nonsense	probably null
R7561:Zfp936	UTSW	7	42,839,339 (GRCm39)	missense	probably damaging	0.98
R7662:Zfp936	UTSW	7	42,839,336 (GRCm39)	nonsense	probably null
R7775:Zfp936	UTSW	7	42,839,720 (GRCm39)	missense	possibly damaging	0.70
R7778:Zfp936	UTSW	7	42,839,720 (GRCm39)	missense	possibly damaging	0.70
R8016:Zfp936	UTSW	7	42,838,848 (GRCm39)	missense	possibly damaging	0.61
R8121:Zfp936	UTSW	7	42,839,547 (GRCm39)	missense	possibly damaging	0.55
R9012:Zfp936	UTSW	7	42,839,416 (GRCm39)	nonsense	probably null
R9058:Zfp936	UTSW	7	42,839,196 (GRCm39)	missense	probably benign	0.32
R9188:Zfp936	UTSW	7	42,839,768 (GRCm39)	missense	probably benign	0.00
R9236:Zfp936	UTSW	7	42,836,922 (GRCm39)	missense	probably benign	0.00
R9596:Zfp936	UTSW	7	42,839,834 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTAATAAAGCCTACCCACAGCAG -3'
(R):5'- AAGCCTTACCACATTGATCACATTT -3'

Sequencing Primer
(F):5'- AGCAGAATTATCTCCGAAAACATG -3'
(R):5'- TTTCATGTACCCGAAGACAGCTGAG -3'

Posted On

2018-11-06