Incidental Mutation 'PIT4519001:Zfp608'
ID 555146
Institutional Source Beutler Lab
Gene Symbol Zfp608
Ensembl Gene ENSMUSG00000052713
Gene Name zinc finger protein 608
Synonyms 4932417D18Rik
Accession Numbers
Essential gene? Probably essential (E-score: 0.899) question?
Stock # PIT4519001 (G1)
Quality Score 225.009
Status Not validated
Chromosome 18
Chromosomal Location 55021120-55125627 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 55079783 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Serine at position 334 (N334S)
Ref Sequence ENSEMBL: ENSMUSP00000068192 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000064763]
AlphaFold Q56A10
Predicted Effect possibly damaging
Transcript: ENSMUST00000064763
AA Change: N334S

PolyPhen 2 Score 0.710 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000068192
Gene: ENSMUSG00000052713
AA Change: N334S

DomainStartEndE-ValueType
low complexity region 51 61 N/A INTRINSIC
low complexity region 150 184 N/A INTRINSIC
low complexity region 214 234 N/A INTRINSIC
low complexity region 252 266 N/A INTRINSIC
low complexity region 309 326 N/A INTRINSIC
low complexity region 428 445 N/A INTRINSIC
low complexity region 459 471 N/A INTRINSIC
ZnF_C2H2 552 577 1.86e0 SMART
low complexity region 624 636 N/A INTRINSIC
low complexity region 706 718 N/A INTRINSIC
low complexity region 734 773 N/A INTRINSIC
low complexity region 807 822 N/A INTRINSIC
low complexity region 833 846 N/A INTRINSIC
low complexity region 934 956 N/A INTRINSIC
coiled coil region 1028 1054 N/A INTRINSIC
low complexity region 1182 1193 N/A INTRINSIC
low complexity region 1255 1268 N/A INTRINSIC
low complexity region 1439 1450 N/A INTRINSIC
low complexity region 1458 1466 N/A INTRINSIC
low complexity region 1486 1502 N/A INTRINSIC
Coding Region Coverage
  • 1x: 93.5%
  • 3x: 90.9%
  • 10x: 84.7%
  • 20x: 70.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2700049A03Rik T A 12: 71,217,440 (GRCm39) M803K probably benign Het
4930590J08Rik C A 6: 91,894,038 (GRCm39) N218K probably damaging Het
Abcc2 G A 19: 43,807,836 (GRCm39) V826M possibly damaging Het
Abcg2 T C 6: 58,651,792 (GRCm39) S395P probably damaging Het
Adam1b C T 5: 121,640,010 (GRCm39) G345D probably damaging Het
Alx4 T A 2: 93,505,773 (GRCm39) C292S probably benign Het
Amd2 C A 10: 35,586,627 (GRCm39) C310F possibly damaging Het
BC051665 A T 13: 60,931,989 (GRCm39) S137T possibly damaging Het
Bmp1 A G 14: 70,727,469 (GRCm39) F705L possibly damaging Het
Coil C T 11: 88,863,552 (GRCm39) probably benign Het
Col6a6 G A 9: 105,609,462 (GRCm39) P1609S probably benign Het
Cpt1b A T 15: 89,303,066 (GRCm39) F633I probably damaging Het
Dcun1d2 A T 8: 13,311,406 (GRCm39) D194E probably benign Het
Defb4 A G 8: 19,248,752 (GRCm39) R2G possibly damaging Het
Dll4 T C 2: 119,162,897 (GRCm39) V506A probably benign Het
Dop1b A G 16: 93,558,942 (GRCm39) S563G probably benign Het
Dpp7 C A 2: 25,242,460 (GRCm39) G498W probably damaging Het
Fam210a A T 18: 68,409,020 (GRCm39) S97T possibly damaging Het
Fastkd1 T A 2: 69,520,501 (GRCm39) D767V probably damaging Het
Fgg A T 3: 82,920,246 (GRCm39) N342Y probably damaging Het
Gimap6 T G 6: 48,684,995 (GRCm39) R30S probably benign Het
Gm10840 T C 11: 106,051,959 (GRCm39) V95A unknown Het
Herc3 T A 6: 58,853,796 (GRCm39) I614N probably damaging Het
Kdr A T 5: 76,097,556 (GRCm39) S1233R possibly damaging Het
Lrp1 C G 10: 127,443,843 (GRCm39) Q141H possibly damaging Het
Magi2 A T 5: 20,866,344 (GRCm39) K1078N probably damaging Het
Meioc A T 11: 102,570,783 (GRCm39) E838V probably damaging Het
Mmp20 A G 9: 7,628,302 (GRCm39) K17E probably benign Het
Mtor C T 4: 148,608,957 (GRCm39) R1538W probably damaging Het
Notch2 C T 3: 98,005,424 (GRCm39) T296I probably damaging Het
Nup98 T C 7: 101,784,171 (GRCm39) S1054G probably benign Het
Or10ac1 T C 6: 42,515,534 (GRCm39) T141A probably damaging Het
Or2a12 T C 6: 42,904,578 (GRCm39) C138R probably damaging Het
Or5b109 A C 19: 13,212,216 (GRCm39) I201L probably benign Het
Or6f1 T C 7: 85,970,941 (GRCm39) Y73C probably damaging Het
Pipox C A 11: 77,774,001 (GRCm39) W205L probably damaging Het
Plin4 T G 17: 56,410,828 (GRCm39) T1068P probably benign Het
Pou6f2 A G 13: 18,414,149 (GRCm39) S209P unknown Het
Ptpdc1 T C 13: 48,736,632 (GRCm39) T713A probably benign Het
Rgsl1 T C 1: 153,701,716 (GRCm39) Y246C possibly damaging Het
Rnf19b C T 4: 128,969,446 (GRCm39) A354V probably damaging Het
Rsph4a A G 10: 33,785,126 (GRCm39) T346A probably benign Het
Rsrc2 T C 5: 123,883,135 (GRCm39) T16A unknown Het
Scrn3 T C 2: 73,148,768 (GRCm39) V113A possibly damaging Het
Scrn3 T A 2: 73,161,347 (GRCm39) I311K possibly damaging Het
Sfmbt1 T C 14: 30,506,148 (GRCm39) probably null Het
Sin3a A G 9: 57,002,740 (GRCm39) I211V possibly damaging Het
Strn3 T C 12: 51,680,491 (GRCm39) T370A probably benign Het
Sulf1 T G 1: 12,918,395 (GRCm39) N786K probably damaging Het
Taf1b T A 12: 24,597,118 (GRCm39) Y352* probably null Het
Tas2r120 T A 6: 132,634,297 (GRCm39) N126K probably benign Het
Tjap1 C A 17: 46,572,432 (GRCm39) R68L probably benign Het
Tnfsf12 C T 11: 69,586,230 (GRCm39) R66Q probably benign Het
Traj45 A G 14: 54,410,303 (GRCm39) D6G Het
Trappc9 T C 15: 72,824,943 (GRCm39) T541A probably benign Het
Ttc29 A C 8: 79,052,106 (GRCm39) E329A probably benign Het
Vmn1r117 T G 7: 20,617,160 (GRCm39) D296A possibly damaging Het
Vmn1r203 A G 13: 22,708,765 (GRCm39) N182S probably benign Het
Vps53 C A 11: 76,007,999 (GRCm39) R287L probably damaging Het
Vwce G A 19: 10,641,946 (GRCm39) E891K possibly damaging Het
Zfp648 A G 1: 154,080,687 (GRCm39) H282R probably damaging Het
Other mutations in Zfp608
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00500:Zfp608 APN 18 55,121,405 (GRCm39) missense probably benign
IGL00920:Zfp608 APN 18 55,022,903 (GRCm39) missense probably benign 0.00
IGL01088:Zfp608 APN 18 55,031,159 (GRCm39) missense probably benign 0.03
IGL01447:Zfp608 APN 18 55,032,083 (GRCm39) missense possibly damaging 0.75
IGL01534:Zfp608 APN 18 55,032,004 (GRCm39) missense probably damaging 0.99
IGL01547:Zfp608 APN 18 55,027,521 (GRCm39) critical splice donor site probably null
IGL01933:Zfp608 APN 18 55,120,871 (GRCm39) missense probably benign
IGL01998:Zfp608 APN 18 55,024,890 (GRCm39) missense probably damaging 0.99
IGL02167:Zfp608 APN 18 55,121,296 (GRCm39) missense probably damaging 1.00
IGL02266:Zfp608 APN 18 55,030,653 (GRCm39) missense probably benign 0.08
IGL02335:Zfp608 APN 18 55,030,509 (GRCm39) nonsense probably null
IGL02455:Zfp608 APN 18 55,032,405 (GRCm39) missense probably damaging 1.00
IGL02612:Zfp608 APN 18 55,031,273 (GRCm39) missense probably damaging 1.00
IGL02900:Zfp608 APN 18 55,079,865 (GRCm39) missense probably damaging 1.00
PIT4243001:Zfp608 UTSW 18 55,031,096 (GRCm39) missense probably damaging 1.00
R0005:Zfp608 UTSW 18 55,028,592 (GRCm39) missense possibly damaging 0.88
R0010:Zfp608 UTSW 18 55,028,286 (GRCm39) splice site probably benign
R0010:Zfp608 UTSW 18 55,028,286 (GRCm39) splice site probably benign
R0064:Zfp608 UTSW 18 55,031,888 (GRCm39) missense probably benign
R0401:Zfp608 UTSW 18 55,032,066 (GRCm39) missense probably benign
R0722:Zfp608 UTSW 18 55,033,306 (GRCm39) missense probably damaging 1.00
R1351:Zfp608 UTSW 18 55,031,463 (GRCm39) missense probably benign 0.00
R1512:Zfp608 UTSW 18 55,079,738 (GRCm39) missense probably damaging 1.00
R1554:Zfp608 UTSW 18 55,031,126 (GRCm39) missense probably damaging 1.00
R1622:Zfp608 UTSW 18 55,121,366 (GRCm39) missense probably benign 0.07
R1669:Zfp608 UTSW 18 55,120,811 (GRCm39) missense probably benign 0.09
R1690:Zfp608 UTSW 18 55,120,706 (GRCm39) missense possibly damaging 0.62
R1721:Zfp608 UTSW 18 55,032,321 (GRCm39) missense probably benign
R1826:Zfp608 UTSW 18 55,031,648 (GRCm39) missense probably benign 0.03
R1864:Zfp608 UTSW 18 55,030,983 (GRCm39) missense probably benign 0.00
R1952:Zfp608 UTSW 18 55,030,851 (GRCm39) nonsense probably null
R2049:Zfp608 UTSW 18 55,028,637 (GRCm39) missense probably damaging 1.00
R2051:Zfp608 UTSW 18 55,121,386 (GRCm39) missense probably benign
R2168:Zfp608 UTSW 18 55,031,125 (GRCm39) nonsense probably null
R2218:Zfp608 UTSW 18 55,120,756 (GRCm39) missense probably benign 0.14
R2283:Zfp608 UTSW 18 55,121,446 (GRCm39) missense probably damaging 1.00
R2399:Zfp608 UTSW 18 55,030,974 (GRCm39) missense probably damaging 1.00
R2520:Zfp608 UTSW 18 55,121,578 (GRCm39) missense possibly damaging 0.92
R2961:Zfp608 UTSW 18 55,031,544 (GRCm39) missense possibly damaging 0.60
R4074:Zfp608 UTSW 18 55,031,180 (GRCm39) missense probably damaging 1.00
R4076:Zfp608 UTSW 18 55,031,180 (GRCm39) missense probably damaging 1.00
R4206:Zfp608 UTSW 18 55,121,267 (GRCm39) missense probably damaging 1.00
R4756:Zfp608 UTSW 18 55,027,544 (GRCm39) missense probably damaging 1.00
R4771:Zfp608 UTSW 18 55,121,372 (GRCm39) missense probably benign
R4820:Zfp608 UTSW 18 55,120,756 (GRCm39) missense probably benign 0.14
R4825:Zfp608 UTSW 18 55,031,041 (GRCm39) missense probably benign 0.01
R4912:Zfp608 UTSW 18 55,079,663 (GRCm39) missense probably damaging 1.00
R4975:Zfp608 UTSW 18 55,022,962 (GRCm39) missense probably damaging 1.00
R5138:Zfp608 UTSW 18 55,024,871 (GRCm39) missense probably damaging 1.00
R5192:Zfp608 UTSW 18 55,031,569 (GRCm39) missense probably damaging 1.00
R5557:Zfp608 UTSW 18 55,120,942 (GRCm39) missense possibly damaging 0.81
R5624:Zfp608 UTSW 18 55,031,344 (GRCm39) missense probably damaging 1.00
R5818:Zfp608 UTSW 18 55,028,468 (GRCm39) missense probably benign 0.02
R5840:Zfp608 UTSW 18 55,031,978 (GRCm39) missense probably damaging 1.00
R5992:Zfp608 UTSW 18 55,032,320 (GRCm39) missense probably benign 0.15
R6106:Zfp608 UTSW 18 55,120,944 (GRCm39) missense possibly damaging 0.54
R6174:Zfp608 UTSW 18 55,121,616 (GRCm39) start gained probably benign
R6181:Zfp608 UTSW 18 55,028,700 (GRCm39) missense possibly damaging 0.56
R6517:Zfp608 UTSW 18 55,032,150 (GRCm39) missense possibly damaging 0.94
R6567:Zfp608 UTSW 18 55,030,628 (GRCm39) missense probably damaging 1.00
R6668:Zfp608 UTSW 18 55,031,091 (GRCm39) missense probably damaging 1.00
R6920:Zfp608 UTSW 18 55,121,337 (GRCm39) missense probably damaging 1.00
R7061:Zfp608 UTSW 18 55,121,069 (GRCm39) missense probably benign
R7074:Zfp608 UTSW 18 55,030,454 (GRCm39) missense possibly damaging 0.69
R7087:Zfp608 UTSW 18 55,032,469 (GRCm39) missense probably damaging 1.00
R7391:Zfp608 UTSW 18 55,030,619 (GRCm39) missense possibly damaging 0.49
R7600:Zfp608 UTSW 18 55,121,092 (GRCm39) missense probably damaging 1.00
R7723:Zfp608 UTSW 18 55,030,673 (GRCm39) missense probably damaging 1.00
R8054:Zfp608 UTSW 18 55,032,618 (GRCm39) missense probably benign 0.28
R8236:Zfp608 UTSW 18 55,032,281 (GRCm39) missense probably damaging 1.00
R8260:Zfp608 UTSW 18 55,030,821 (GRCm39) missense possibly damaging 0.60
R8732:Zfp608 UTSW 18 55,121,072 (GRCm39) missense probably benign 0.06
R8781:Zfp608 UTSW 18 55,031,801 (GRCm39) missense probably damaging 1.00
R8851:Zfp608 UTSW 18 55,032,194 (GRCm39) missense possibly damaging 0.94
R8940:Zfp608 UTSW 18 55,033,301 (GRCm39) missense possibly damaging 0.93
R9051:Zfp608 UTSW 18 55,032,266 (GRCm39) missense probably damaging 1.00
R9091:Zfp608 UTSW 18 55,032,190 (GRCm39) missense probably damaging 1.00
R9092:Zfp608 UTSW 18 55,031,648 (GRCm39) missense probably benign 0.03
R9236:Zfp608 UTSW 18 55,032,354 (GRCm39) missense probably benign 0.39
R9270:Zfp608 UTSW 18 55,032,190 (GRCm39) missense probably damaging 1.00
R9283:Zfp608 UTSW 18 55,030,913 (GRCm39) missense possibly damaging 0.50
R9288:Zfp608 UTSW 18 55,033,341 (GRCm39) missense probably benign 0.39
R9463:Zfp608 UTSW 18 55,030,274 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCTCACAGATGGACAGAGATAC -3'
(R):5'- AGCGATGTAAGCAGATTGCATTAC -3'

Sequencing Primer
(F):5'- TCACAGATGGACAGAGATACATCATG -3'
(R):5'- GCAGATTGCATTACAGAGAACTC -3'
Posted On 2019-06-07