Mutagenetix > Incidental Mutation

Incidental Mutation 'R7533:Zfp61'

583413

Institutional Source

Beutler Lab

Gene Symbol

Zfp61

Ensembl Gene

ENSMUSG00000050605

Gene Name

zinc finger protein 61

Synonyms

MMRRC Submission

045605-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R7533 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23990464-24000657 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23990504 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Arginine at position 549 (Q549R)

Ref Sequence

ENSEMBL: ENSMUSP00000076954 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077780] [ENSMUST00000145131] [ENSMUST00000165241]

AlphaFold

Q923D1

Predicted Effect

probably benign
Transcript: ENSMUST00000077780
AA Change: Q549R

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000076954
Gene: ENSMUSG00000050605
AA Change: Q549R

Domain	Start	End	E-Value	Type
KRAB	4	63	2.28e-27	SMART
ZnF_C2H2	311	333	1.4e-4	SMART
ZnF_C2H2	339	361	5.5e-3	SMART
ZnF_C2H2	367	389	2.99e-4	SMART
ZnF_C2H2	395	417	1.18e-2	SMART
ZnF_C2H2	423	445	2.4e-3	SMART
ZnF_C2H2	451	473	2.95e-3	SMART
ZnF_C2H2	479	501	9.88e-5	SMART
ZnF_C2H2	507	529	1.5e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000145131

SMART Domains

Protein: ENSMUSP00000117077
Gene: ENSMUSG00000050605

Domain	Start	End	E-Value	Type
KRAB	4	64	4.63e-19	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000165241
AA Change: Q549R

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000132551
Gene: ENSMUSG00000050605
AA Change: Q549R

Domain	Start	End	E-Value	Type
KRAB	4	63	2.28e-27	SMART
ZnF_C2H2	311	333	1.4e-4	SMART
ZnF_C2H2	339	361	5.5e-3	SMART
ZnF_C2H2	367	389	2.99e-4	SMART
ZnF_C2H2	395	417	1.18e-2	SMART
ZnF_C2H2	423	445	2.4e-3	SMART
ZnF_C2H2	451	473	2.95e-3	SMART
ZnF_C2H2	479	501	9.88e-5	SMART
ZnF_C2H2	507	529	1.5e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agr2	T	C	12: 36,046,128 (GRCm39)		probably null	Het
Alpk2	A	T	18: 65,437,674 (GRCm39)	S1240T	probably damaging	Het
Atp8b2	G	A	3: 89,852,831 (GRCm39)	L144F		Het
Cck	T	C	9: 121,319,201 (GRCm39)	D94G	probably damaging	Het
Ccser1	A	T	6: 61,615,474 (GRCm39)	T659S	probably benign	Het
Cfap74	C	A	4: 155,500,200 (GRCm39)	D15E		Het
Commd5	G	A	15: 76,784,776 (GRCm39)	A58T	probably benign	Het
Dctn2	A	G	10: 127,103,347 (GRCm39)	D34G	possibly damaging	Het
Ecpas	T	C	4: 58,809,411 (GRCm39)	I1654M	probably benign	Het
Epha6	T	C	16: 60,025,925 (GRCm39)	T506A	probably damaging	Het
Fat4	A	C	3: 39,061,406 (GRCm39)	I4330L	probably benign	Het
Fcho2	G	T	13: 98,921,307 (GRCm39)	Q193K	probably benign	Het
Hace1	A	T	10: 45,587,570 (GRCm39)	I890L	probably benign	Het
Igsf11	T	G	16: 38,829,236 (GRCm39)	N102K	probably benign	Het
Il7r	G	A	15: 9,508,047 (GRCm39)	S425L	probably benign	Het
Itga3	G	T	11: 94,937,344 (GRCm39)	Q1011K	probably benign	Het
Kif3c	T	A	12: 3,416,510 (GRCm39)	I177N	probably damaging	Het
Neb	G	A	2: 52,114,578 (GRCm39)	A1048V		Het
Neu4	C	T	1: 93,950,122 (GRCm39)	T27M	probably benign	Het
Or52b3	T	C	7: 102,203,718 (GRCm39)	S76P	probably damaging	Het
P4htm	T	C	9: 108,474,136 (GRCm39)	H134R	probably benign	Het
Padi6	T	C	4: 140,458,506 (GRCm39)	D462G	probably damaging	Het
Pi4ka	T	C	16: 17,115,525 (GRCm39)	D1375G		Het
Ptgr1	C	T	4: 58,971,011 (GRCm39)	R247H	possibly damaging	Het
Rad50	T	C	11: 53,589,746 (GRCm39)	N152D	probably damaging	Het
Serpina1c	T	A	12: 103,863,566 (GRCm39)	K211N	probably damaging	Het
Sgsh	T	C	11: 119,238,696 (GRCm39)	E256G	probably damaging	Het
Tnrc6b	T	C	15: 80,811,942 (GRCm39)		probably null	Het
Ube3d	A	G	9: 86,304,990 (GRCm39)	F284S	probably damaging	Het
Usp13	A	G	3: 32,973,091 (GRCm39)	D757G	probably damaging	Het
Vmn1r173	C	T	7: 23,402,071 (GRCm39)	A102V	probably benign	Het
Vmn2r29	G	T	7: 7,244,656 (GRCm39)	A406D	probably damaging	Het
Wdfy3	G	A	5: 102,030,354 (GRCm39)	R2385C	probably benign	Het
Zfp112	A	T	7: 23,824,752 (GRCm39)	K244M	possibly damaging	Het

Other mutations in Zfp61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00588:Zfp61	APN	7	23,990,520 (GRCm39)	missense	probably benign	0.00
R0771:Zfp61	UTSW	7	23,992,779 (GRCm39)	missense	probably benign	0.00
R1574:Zfp61	UTSW	7	23,990,635 (GRCm39)	missense	probably damaging	0.98
R1574:Zfp61	UTSW	7	23,990,635 (GRCm39)	missense	probably damaging	0.98
R2029:Zfp61	UTSW	7	23,991,714 (GRCm39)	missense	probably benign	0.00
R2443:Zfp61	UTSW	7	23,991,194 (GRCm39)	missense	probably benign	0.01
R3771:Zfp61	UTSW	7	23,995,406 (GRCm39)	start codon destroyed	probably null	0.01
R3773:Zfp61	UTSW	7	23,995,406 (GRCm39)	start codon destroyed	probably null	0.01
R4093:Zfp61	UTSW	7	23,990,700 (GRCm39)	splice site	probably null
R4095:Zfp61	UTSW	7	23,990,700 (GRCm39)	splice site	probably null
R8423:Zfp61	UTSW	7	23,991,459 (GRCm39)	missense	probably benign	0.18
R9057:Zfp61	UTSW	7	23,990,702 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCTCTTGCAAAGGAAGAACCC -3'
(R):5'- CTCTGAATTCAAATCTCCAAGTCC -3'

Sequencing Primer
(F):5'- TCCCAAACCAAACTATTGTAAAGTG -3'
(R):5'- GGAAAGGCTTCAGTCGCTCTTC -3'

Posted On

2019-10-17