Mutagenetix > Incidental Mutation

Incidental Mutation 'R0018:Stk24'

64954

Institutional Source

Beutler Lab

Gene Symbol

Stk24

Ensembl Gene

ENSMUSG00000063410

Gene Name

serine/threonine kinase 24

Synonyms

1810013H02Rik, STE20

MMRRC Submission

038313-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.348) question?

Stock #

R0018 (G1)

Quality Score

139

Status

Validated

Chromosome

Chromosomal Location

121523755-121617423 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 121545419 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000078746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079817]

AlphaFold

Q99KH8

Predicted Effect

probably benign
Transcript: ENSMUST00000079817

SMART Domains

Protein: ENSMUSP00000078746
Gene: ENSMUSG00000063410

Domain	Start	End	E-Value	Type
S_TKc	24	274	3.18e-99	SMART
low complexity region	297	324	N/A	INTRINSIC
PDB:3W8H\|B	356	422	1e-20	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000226161

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227273

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 99.0%
10x: 97.9%
20x: 96.6%

Validation Efficiency

100% (64/64)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
PHENOTYPE: A hypomorphic mutation increases degranulation of, and exocytosis by, neutrophils. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	T	C	17: 24,532,162 (GRCm39)		probably null	Het
Afp	A	C	5: 90,654,600 (GRCm39)	Q546P	probably damaging	Het
Api5	A	T	2: 94,251,329 (GRCm39)		probably null	Het
Atp2b4	T	A	1: 133,645,609 (GRCm39)	I982F	probably damaging	Het
BC024139	G	A	15: 76,005,087 (GRCm39)	Q592*	probably null	Het
Capn7	T	A	14: 31,076,069 (GRCm39)	C290*	probably null	Het
Ccn1	A	G	3: 145,355,186 (GRCm39)	L23P	probably damaging	Het
Celsr1	T	A	15: 85,915,243 (GRCm39)	D910V	possibly damaging	Het
Chga	T	C	12: 102,524,764 (GRCm39)	S45P	probably damaging	Het
Cpne2	T	A	8: 95,282,681 (GRCm39)	C59S	possibly damaging	Het
Cyp2b13	G	A	7: 25,785,375 (GRCm39)	R248H	probably benign	Het
Dennd1a	T	A	2: 37,748,472 (GRCm39)	T336S	possibly damaging	Het
Drc7	A	G	8: 95,800,862 (GRCm39)	Y628C	probably damaging	Het
Dse	A	G	10: 34,029,464 (GRCm39)	V542A	probably benign	Het
Dspp	A	T	5: 104,326,096 (GRCm39)	S820C	unknown	Het
Efcab3	G	A	11: 104,612,378 (GRCm39)		probably null	Het
Eif5b	T	C	1: 38,057,970 (GRCm39)	S91P	unknown	Het
Epop	A	G	11: 97,519,017 (GRCm39)	V364A	probably benign	Het
Ext2	G	A	2: 93,626,037 (GRCm39)	P341L	probably damaging	Het
Galns	T	C	8: 123,311,724 (GRCm39)	T429A	probably benign	Het
Gsx2	A	G	5: 75,237,828 (GRCm39)	K260R	probably damaging	Het
H2-M10.6	A	C	17: 37,124,941 (GRCm39)	H286P	probably damaging	Het
Hectd4	A	G	5: 121,392,242 (GRCm39)	N169D	possibly damaging	Het
Helz2	C	A	2: 180,874,552 (GRCm39)	G1981C	probably damaging	Het
Hmcn1	T	C	1: 150,528,302 (GRCm39)	D3282G	probably benign	Het
Hnmt	T	A	2: 23,893,640 (GRCm39)	N285Y	possibly damaging	Het
Hr	A	G	14: 70,795,717 (GRCm39)	R450G	probably benign	Het
Kat6a	A	G	8: 23,419,289 (GRCm39)	D684G	possibly damaging	Het
Kif27	T	G	13: 58,435,867 (GRCm39)	I1309L	probably benign	Het
Mab21l4	T	A	1: 93,082,327 (GRCm39)	D264V	probably benign	Het
Man2b1	T	A	8: 85,824,118 (GRCm39)	V1005E	probably damaging	Het
Me2	A	T	18: 73,924,923 (GRCm39)	F265I	possibly damaging	Het
Myo9a	A	T	9: 59,779,007 (GRCm39)	T1588S	probably benign	Het
Neu4	T	A	1: 93,953,060 (GRCm39)	D476E	probably benign	Het
Nlrp9c	T	A	7: 26,071,423 (GRCm39)	Q895L	possibly damaging	Het
Or1e35	T	C	11: 73,797,452 (GRCm39)	I289V	probably damaging	Het
Or1p1c	A	C	11: 74,160,934 (GRCm39)	T240P	probably benign	Het
Pdzd8	C	T	19: 59,289,105 (GRCm39)	R765H	probably damaging	Het
Plk1	T	C	7: 121,768,208 (GRCm39)		probably null	Het
Ppfia2	A	G	10: 106,678,647 (GRCm39)		probably benign	Het
Prkdc	T	C	16: 15,544,406 (GRCm39)	Y1799H	probably benign	Het
Psmc1	T	C	12: 100,082,951 (GRCm39)		probably benign	Het
Ptchd3	A	C	11: 121,733,170 (GRCm39)	I687L	probably benign	Het
Ptprh	A	G	7: 4,604,845 (GRCm39)		probably null	Het
Pus3	A	G	9: 35,477,920 (GRCm39)	D384G	probably benign	Het
Rab44	C	T	17: 29,358,354 (GRCm39)	P181S	probably benign	Het
Rasa2	A	G	9: 96,454,016 (GRCm39)	S307P	probably damaging	Het
Rbpms2	A	G	9: 65,558,360 (GRCm39)	D142G	probably damaging	Het
Reln	A	T	5: 22,130,369 (GRCm39)	D2647E	probably benign	Het
Ryr2	G	A	13: 11,610,109 (GRCm39)	T4239I	possibly damaging	Het
Sctr	C	A	1: 119,971,286 (GRCm39)		probably benign	Het
Serpinb6e	A	T	13: 34,021,828 (GRCm39)	Y167N	probably damaging	Het
Slc13a5	T	C	11: 72,157,301 (GRCm39)	I31V	probably benign	Het
Slc15a4	A	T	5: 127,679,074 (GRCm39)	I422N	probably damaging	Het
Vmn1r213	T	A	13: 23,196,311 (GRCm39)	V298D	probably damaging	Het
Xdh	C	T	17: 74,232,020 (GRCm39)	R230H	probably benign	Het
Zfp418	A	T	7: 7,185,449 (GRCm39)	S471C	probably benign	Het

Other mutations in Stk24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01432:Stk24	APN	14	121,540,218 (GRCm39)	missense	probably damaging	1.00
IGL02886:Stk24	APN	14	121,529,527 (GRCm39)	missense	probably null	1.00
IGL03278:Stk24	APN	14	121,540,182 (GRCm39)	missense	possibly damaging	0.88
Megatron	UTSW	14	121,545,419 (GRCm39)	splice site	probably benign
R1309:Stk24	UTSW	14	121,540,198 (GRCm39)	missense	probably damaging	0.99
R1446:Stk24	UTSW	14	121,545,456 (GRCm39)	missense	probably damaging	1.00
R1567:Stk24	UTSW	14	121,545,468 (GRCm39)	missense	probably benign	0.00
R1673:Stk24	UTSW	14	121,574,983 (GRCm39)	missense	probably damaging	1.00
R2131:Stk24	UTSW	14	121,539,623 (GRCm39)	missense	probably damaging	1.00
R4302:Stk24	UTSW	14	121,529,494 (GRCm39)	missense	probably benign	0.07
R4716:Stk24	UTSW	14	121,532,130 (GRCm39)	missense	possibly damaging	0.85
R4865:Stk24	UTSW	14	121,530,866 (GRCm39)	nonsense	probably null
R5381:Stk24	UTSW	14	121,531,645 (GRCm39)	missense	possibly damaging	0.80
R5540:Stk24	UTSW	14	121,531,693 (GRCm39)	missense	possibly damaging	0.69
R6017:Stk24	UTSW	14	121,539,657 (GRCm39)	missense	probably benign	0.15
R6913:Stk24	UTSW	14	121,540,221 (GRCm39)	missense	probably damaging	1.00
R7081:Stk24	UTSW	14	121,531,706 (GRCm39)	missense	probably benign	0.01
R7251:Stk24	UTSW	14	121,545,434 (GRCm39)	missense	probably damaging	1.00
R7586:Stk24	UTSW	14	121,539,699 (GRCm39)	missense	probably damaging	0.99
R7587:Stk24	UTSW	14	121,539,699 (GRCm39)	missense	probably damaging	0.99
R7771:Stk24	UTSW	14	121,575,045 (GRCm39)	missense	probably damaging	1.00
R8288:Stk24	UTSW	14	121,530,841 (GRCm39)	missense	possibly damaging	0.47
R8528:Stk24	UTSW	14	121,529,447 (GRCm39)	missense	probably benign	0.01
RF008:Stk24	UTSW	14	121,532,172 (GRCm39)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- TATAGTGAAATGGCCCGCTCTCCC -3'
(R):5'- TTCAAGACGGAAACGCCCTCAG -3'

Sequencing Primer
(F):5'- TCCCAGTGGCCTCACAAG -3'
(R):5'- TCAGTCCTGCCCTGAAGATAC -3'

Posted On

2013-08-06