Mutagenetix > Incidental Mutation

Incidental Mutation 'R1071:Zfp786'

86191

Institutional Source

Beutler Lab

Gene Symbol

Zfp786

Ensembl Gene

ENSMUSG00000051499

Gene Name

zinc finger protein 786

Synonyms

A730012O14Rik

MMRRC Submission

039157-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R1071 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

47796200-47807801 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 47798239 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 233 (D233G)

Ref Sequence

ENSEMBL: ENSMUSP00000057495 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058844]

AlphaFold

Q8BV42

Predicted Effect

possibly damaging
Transcript: ENSMUST00000058844
AA Change: D233G

PolyPhen 2 Score 0.906 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000057495
Gene: ENSMUSG00000051499
AA Change: D233G

Domain	Start	End	E-Value	Type
KRAB	9	69	2.6e-22	SMART
low complexity region	156	164	N/A	INTRINSIC
ZnF_C2H2	240	262	5.34e-1	SMART
ZnF_C2H2	268	295	1.41e2	SMART
ZnF_C2H2	420	440	1.21e2	SMART
ZnF_C2H2	448	470	3.52e-1	SMART
ZnF_C2H2	476	498	1.89e-1	SMART
ZnF_C2H2	504	526	1.69e-3	SMART
ZnF_C2H2	532	554	5.59e-4	SMART
ZnF_C2H2	560	582	4.79e-3	SMART
ZnF_C2H2	588	610	3.69e-4	SMART
ZnF_C2H2	616	638	3.63e-3	SMART
ZnF_C2H2	644	665	5.92e0	SMART
ZnF_C2H2	671	693	2.57e-3	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	1.45e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143979

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.3%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 13 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apc2	T	C	10: 80,147,336 (GRCm39)	S768P	probably damaging	Het
Bub1b	CAT	C	2: 118,462,928 (GRCm39)		probably null	Het
Cplane1	T	A	15: 8,247,910 (GRCm39)	H1486Q	probably benign	Het
Eml4	G	T	17: 83,785,468 (GRCm39)	E878*	probably null	Het
Plcb1	A	G	2: 135,167,577 (GRCm39)	D457G	possibly damaging	Het
Prepl	T	C	17: 85,377,940 (GRCm39)	Y480C	probably damaging	Het
Senp6	T	A	9: 80,044,011 (GRCm39)	Y708N	probably benign	Het
Ugt2b38	T	G	5: 87,560,232 (GRCm39)	N361H	probably damaging	Het
Ugt3a1	T	C	15: 9,367,454 (GRCm39)	V399A	possibly damaging	Het
Vmn1r30	A	T	6: 58,412,813 (GRCm39)	N6K	possibly damaging	Het
Vmn2r3	G	A	3: 64,182,697 (GRCm39)	T334I	possibly damaging	Het
Vtn	C	A	11: 78,392,602 (GRCm39)	N393K	probably benign	Het
Zmym2	A	G	14: 57,197,278 (GRCm39)	T1349A	possibly damaging	Het

Other mutations in Zfp786

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Zfp786	APN	6	47,796,605 (GRCm39)	makesense	probably null
IGL02442:Zfp786	APN	6	47,798,301 (GRCm39)	missense	probably benign	0.00
IGL02674:Zfp786	APN	6	47,797,427 (GRCm39)	missense	probably benign	0.22
IGL02814:Zfp786	APN	6	47,796,775 (GRCm39)	missense	probably damaging	1.00
IGL03072:Zfp786	APN	6	47,798,177 (GRCm39)	missense	probably benign	0.01
IGL03294:Zfp786	APN	6	47,798,258 (GRCm39)	nonsense	probably null
IGL03393:Zfp786	APN	6	47,798,458 (GRCm39)	missense	possibly damaging	0.88
3-1:Zfp786	UTSW	6	47,797,379 (GRCm39)	missense	probably damaging	1.00
PIT4581001:Zfp786	UTSW	6	47,796,756 (GRCm39)	missense	probably damaging	1.00
R1426:Zfp786	UTSW	6	47,802,013 (GRCm39)	missense	probably benign	0.17
R1976:Zfp786	UTSW	6	47,796,691 (GRCm39)	missense	probably damaging	1.00
R2114:Zfp786	UTSW	6	47,803,931 (GRCm39)	missense	probably damaging	1.00
R2115:Zfp786	UTSW	6	47,803,931 (GRCm39)	missense	probably damaging	1.00
R2117:Zfp786	UTSW	6	47,803,931 (GRCm39)	missense	probably damaging	1.00
R2176:Zfp786	UTSW	6	47,797,905 (GRCm39)	missense	possibly damaging	0.65
R2304:Zfp786	UTSW	6	47,797,633 (GRCm39)	missense	probably damaging	1.00
R3110:Zfp786	UTSW	6	47,797,160 (GRCm39)	missense	probably damaging	1.00
R3112:Zfp786	UTSW	6	47,797,160 (GRCm39)	missense	probably damaging	1.00
R3623:Zfp786	UTSW	6	47,798,357 (GRCm39)	missense	probably benign	0.00
R4654:Zfp786	UTSW	6	47,797,868 (GRCm39)	missense	probably benign	0.01
R4741:Zfp786	UTSW	6	47,797,625 (GRCm39)	missense	probably damaging	1.00
R4927:Zfp786	UTSW	6	47,797,087 (GRCm39)	missense	probably benign	0.43
R4936:Zfp786	UTSW	6	47,798,202 (GRCm39)	nonsense	probably null
R5084:Zfp786	UTSW	6	47,796,953 (GRCm39)	missense	probably benign	0.00
R5445:Zfp786	UTSW	6	47,796,619 (GRCm39)	missense	probably damaging	1.00
R6662:Zfp786	UTSW	6	47,803,920 (GRCm39)	missense	probably damaging	1.00
R7290:Zfp786	UTSW	6	47,796,929 (GRCm39)	missense	probably damaging	1.00
R7561:Zfp786	UTSW	6	47,796,667 (GRCm39)	missense	probably benign	0.02
R8038:Zfp786	UTSW	6	47,798,188 (GRCm39)	missense	probably benign	0.01
R8090:Zfp786	UTSW	6	47,796,943 (GRCm39)	missense	probably damaging	1.00
R8250:Zfp786	UTSW	6	47,797,729 (GRCm39)	missense	possibly damaging	0.85
R8438:Zfp786	UTSW	6	47,796,934 (GRCm39)	missense	probably damaging	1.00
R8516:Zfp786	UTSW	6	47,797,477 (GRCm39)	missense	probably damaging	1.00
R8868:Zfp786	UTSW	6	47,802,015 (GRCm39)	missense	probably damaging	0.99
R9596:Zfp786	UTSW	6	47,797,406 (GRCm39)	nonsense	probably null
R9788:Zfp786	UTSW	6	47,797,816 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGGGTACGTTCATGATGGAAGCAC -3'
(R):5'- TCTTGTAAACGCACAAACACGCTG -3'

Sequencing Primer
(F):5'- AGCACATTTTGCACTTAGGGC -3'
(R):5'- TCAGTGGTGCATAGCTCCAG -3'

Posted On

2013-11-18