Incidental Mutation 'R1212:Il25'
ID 99454
Institutional Source Beutler Lab
Gene Symbol Il25
Ensembl Gene ENSMUSG00000040770
Gene Name interleukin 25
Synonyms Il17e, IL-17E
MMRRC Submission 039281-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R1212 (G1)
Quality Score 122
Status Not validated
Chromosome 14
Chromosomal Location 55170152-55173294 bp(+) (GRCm39)
Type of Mutation unclassified
DNA Base Change (assembly) G to A at 55170212 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000153874 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000037814] [ENSMUST00000037863] [ENSMUST00000227441]
AlphaFold Q8VHH8
Predicted Effect probably benign
Transcript: ENSMUST00000037814
SMART Domains Protein: ENSMUSP00000036138
Gene: ENSMUSG00000040759

DomainStartEndE-ValueType
transmembrane domain 35 56 N/A INTRINSIC
transmembrane domain 60 82 N/A INTRINSIC
transmembrane domain 95 114 N/A INTRINSIC
transmembrane domain 119 136 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000037863
SMART Domains Protein: ENSMUSP00000039641
Gene: ENSMUSG00000040770

DomainStartEndE-ValueType
signal peptide 1 16 N/A INTRINSIC
Pfam:IL17 77 163 4.2e-23 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000227441
Coding Region Coverage
  • 1x: 98.7%
  • 3x: 97.4%
  • 10x: 93.2%
  • 20x: 82.9%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
PHENOTYPE: Mice homozygous for a null allele have increased susceptibility to parasitic infection. Male homozygous mutant mice exhibit notably increased mean serum triglyceride levels in another null allele. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 14 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ambra1 G A 2: 91,599,381 (GRCm39) R167H possibly damaging Het
Ankrd50 G A 3: 38,509,836 (GRCm39) R844C probably damaging Het
Arfgef1 T C 1: 10,286,784 (GRCm39) E42G probably benign Het
Arhgap42 T A 9: 9,015,313 (GRCm39) I444F probably damaging Het
Cspg4b G A 13: 113,505,951 (GRCm39) probably benign Het
Ift56 A G 6: 38,387,728 (GRCm39) D393G probably damaging Het
Ift70a2 A T 2: 75,806,823 (GRCm39) I563N probably damaging Het
Jak1 A G 4: 101,046,291 (GRCm39) L105P probably damaging Het
Or2ad1 C T 13: 21,326,337 (GRCm39) V297I probably benign Het
Ralgapa2 A G 2: 146,199,902 (GRCm39) V1214A probably benign Het
Shtn1 T C 19: 59,039,322 (GRCm39) D39G probably damaging Het
Supt16 G A 14: 52,411,581 (GRCm39) R601* probably null Het
Vmn1r113 A T 7: 20,521,356 (GRCm39) R49S probably benign Het
Vmn1r64 C T 7: 5,887,210 (GRCm39) S111N probably damaging Het
Other mutations in Il25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03327:Il25 APN 14 55,172,817 (GRCm39) unclassified probably benign
R0035:Il25 UTSW 14 55,170,553 (GRCm39) missense probably damaging 0.99
R0368:Il25 UTSW 14 55,172,631 (GRCm39) splice site probably null
R6733:Il25 UTSW 14 55,170,490 (GRCm39) missense probably benign 0.07
R7044:Il25 UTSW 14 55,170,615 (GRCm39) missense probably benign
R7464:Il25 UTSW 14 55,170,679 (GRCm39) missense probably null 1.00
R7488:Il25 UTSW 14 55,170,459 (GRCm39) missense probably benign
Z1177:Il25 UTSW 14 55,172,664 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ATGCCATCCCACAGAGACAGAATTG -3'
(R):5'- AATGAGAAAGCTACTCGCAGGTCAC -3'

Sequencing Primer
(F):5'- TACAACTTCCATGAGGGTAGC -3'
(R):5'- CGCAGGTCACTTGCAGTTC -3'
Posted On 2014-01-15