Mutagenetix > Incidental Mutation

Incidental Mutation 'R9795:Gstt1'

734892

Institutional Source

Beutler Lab

Gene Symbol

Gstt1

Ensembl Gene

ENSMUSG00000001663

Gene Name

glutathione S-transferase, theta 1

Synonyms

Gstt1-1

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.182) question?

Stock #

R9795 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

75619647-75634418 bp(-) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 75634391 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000117130 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001713] [ENSMUST00000120177] [ENSMUST00000139724]

AlphaFold

Q64471

Predicted Effect

probably benign
Transcript: ENSMUST00000001713

SMART Domains

Protein: ENSMUSP00000001713
Gene: ENSMUSG00000001663

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	76	7.5e-13	PFAM
Pfam:GST_N_3	5	83	1.5e-11	PFAM
Pfam:GST_N_2	12	77	1.1e-7	PFAM
Pfam:GST_C_3	88	200	6.2e-10	PFAM
Pfam:GST_C	111	201	3.7e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000120177

SMART Domains

Protein: ENSMUSP00000113839
Gene: ENSMUSG00000001663

Domain	Start	End	E-Value	Type
Pfam:GST_N	8	68	5.8e-11	PFAM
Pfam:GST_N_3	20	77	7.3e-9	PFAM
Pfam:GST_C	103	193	5e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000139724

SMART Domains

Protein: ENSMUSP00000117130
Gene: ENSMUSG00000001663

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	26	4.6e-7	PFAM
Pfam:GST_C	61	126	8.1e-7	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.6%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mis homozygous for disruption of this gene appear to be normal and fertile with the exception of a reduced ability to clear 11,2-bis(2-chloroethyl)-1-nitrosourea from the plasma after a single i.p. dose. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts20	C	T	15: 94,301,180 (GRCm39)	G115R	possibly damaging	Het
Adgrl3	T	C	5: 81,837,421 (GRCm39)	V701A	probably damaging	Het
Afap1l1	T	C	18: 61,874,822 (GRCm39)	D453G	possibly damaging	Het
Ano1	A	T	7: 144,175,434 (GRCm39)	W495R	probably damaging	Het
Apc	T	A	18: 34,447,628 (GRCm39)	L1508Q	probably damaging	Het
Bbs12	C	T	3: 37,374,224 (GRCm39)	T224I	possibly damaging	Het
Cald1	A	T	6: 34,723,071 (GRCm39)	M52L		Het
Calhm6	T	C	10: 34,002,544 (GRCm39)	I180V	probably damaging	Het
Ccdc9b	T	C	2: 118,587,784 (GRCm39)	S517G	unknown	Het
Cdk12	T	A	11: 98,102,051 (GRCm39)	D636E	unknown	Het
Cfap53	A	G	18: 74,438,741 (GRCm39)	D306G	probably benign	Het
Cntnap4	T	C	8: 113,608,357 (GRCm39)	V1259A	probably benign	Het
Cntnap5c	A	T	17: 58,409,192 (GRCm39)	T477S	probably benign	Het
Epha3	T	C	16: 63,372,910 (GRCm39)	E931G	probably benign	Het
Foxred1	CGGG	CGG	9: 35,122,152 (GRCm39)		probably null	Het
Hao1	T	A	2: 134,372,552 (GRCm39)	Y152F	possibly damaging	Het
Hcn4	C	T	9: 58,760,762 (GRCm39)	Q436*	probably null	Het
Hmcn1	G	A	1: 150,608,689 (GRCm39)	P1498S	possibly damaging	Het
Igkv4-79	A	G	6: 69,020,169 (GRCm39)	S49P	probably damaging	Het
Krt73	C	A	15: 101,710,725 (GRCm39)	R3L	probably damaging	Het
Lrriq3	T	C	3: 154,893,313 (GRCm39)	M338T	probably benign	Het
Ltbp2	A	T	12: 84,876,128 (GRCm39)	I493N	probably damaging	Het
Ly6e	G	T	15: 74,830,390 (GRCm39)	C80F	probably damaging	Het
Map1a	T	C	2: 121,121,304 (GRCm39)		probably null	Het
Mitf	A	G	6: 97,970,143 (GRCm39)	H137R	probably benign	Het
Nr1h4	T	G	10: 89,314,651 (GRCm39)	T286P	probably benign	Het
Nsd2	A	G	5: 34,003,489 (GRCm39)	D213G	possibly damaging	Het
Nuggc	T	C	14: 65,847,345 (GRCm39)	S131P	probably damaging	Het
Nwd2	A	G	5: 63,964,232 (GRCm39)	E1272G	probably damaging	Het
Or2y1	A	T	11: 49,385,882 (GRCm39)	N174I	probably damaging	Het
Or4a78	T	C	2: 89,497,811 (GRCm39)	I140V	probably benign	Het
Or4f14c	T	C	2: 111,941,330 (GRCm39)	H89R	probably benign	Het
Or5ac17	T	A	16: 59,036,938 (GRCm39)	I13F	possibly damaging	Het
Or5p79	A	G	7: 108,221,869 (GRCm39)	I283M	probably benign	Het
Or7e170	T	A	9: 19,795,347 (GRCm39)	M85L	probably benign	Het
Or7g32	T	C	9: 19,408,412 (GRCm39)	Y123H	probably damaging	Het
Or8h9	T	A	2: 86,789,119 (GRCm39)	I228F	probably damaging	Het
Pacsin3	C	A	2: 91,094,160 (GRCm39)	A363D	probably benign	Het
Pcdh17	C	T	14: 84,770,350 (GRCm39)	R943*	probably null	Het
Pkhd1l1	T	C	15: 44,406,983 (GRCm39)	S2407P	probably benign	Het
Ppip5k2	A	T	1: 97,671,822 (GRCm39)	Y489*	probably null	Het
Qrich1	T	A	9: 108,411,089 (GRCm39)	S205T	probably benign	Het
Rasgrp1	T	C	2: 117,118,429 (GRCm39)	D520G	probably benign	Het
Rbm20	C	A	19: 53,852,551 (GRCm39)	T1177K	probably benign	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Sel1l3	C	A	5: 53,329,924 (GRCm39)	R477L	probably benign	Het
Slc27a3	C	T	3: 90,296,875 (GRCm39)	W32*	probably null	Het
Slc5a8	T	C	10: 88,757,591 (GRCm39)	I527T	possibly damaging	Het
Slc6a7	C	A	18: 61,138,866 (GRCm39)	R214L	probably benign	Het
Stx7	T	C	10: 24,057,475 (GRCm39)	L167P	probably damaging	Het
Sult1d1	T	A	5: 87,712,655 (GRCm39)	N63I	probably damaging	Het
Ttc23l	T	A	15: 10,537,731 (GRCm39)	I180F	probably benign	Het
Usp31	A	G	7: 121,247,499 (GRCm39)	S1315P	probably benign	Het
Usp9y	T	C	Y: 1,364,679 (GRCm39)	M1045V	probably benign	Het
Vmn2r118	T	C	17: 55,899,496 (GRCm39)	T803A	probably damaging	Het
Vmn2r97	T	A	17: 19,167,561 (GRCm39)	V605D	probably damaging	Het
Wdr26	A	G	1: 181,036,812 (GRCm39)	F143S	probably damaging	Het
Zfp51	T	G	17: 21,682,051 (GRCm39)		probably null	Het

Other mutations in Gstt1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01635:Gstt1	APN	10	75,629,951 (GRCm39)	missense	probably damaging	1.00
R1460:Gstt1	UTSW	10	75,620,004 (GRCm39)	missense	probably damaging	0.99
R1646:Gstt1	UTSW	10	75,619,940 (GRCm39)	missense	possibly damaging	0.91
R4603:Gstt1	UTSW	10	75,629,969 (GRCm39)	missense	probably damaging	1.00
R6757:Gstt1	UTSW	10	75,634,217 (GRCm39)	critical splice donor site	probably null
R7896:Gstt1	UTSW	10	75,619,976 (GRCm39)	missense	probably damaging	1.00
R8459:Gstt1	UTSW	10	75,629,943 (GRCm39)	missense	probably damaging	1.00
R9792:Gstt1	UTSW	10	75,634,391 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- TCAGATCCCCAAAGCCGTAG -3'
(R):5'- TCTGCTTTCAACTAAACCGGCC -3'

Sequencing Primer
(F):5'- AAAGCCGTAGCCCTGCC -3'
(R):5'- CGGCCACAAGAGGACTAGC -3'

Posted On

2022-11-14