Incidental Mutation 'R6757:Gstt1'
| ID |
531093 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gstt1
|
| Ensembl Gene |
ENSMUSG00000001663 |
| Gene Name |
glutathione S-transferase, theta 1 |
| Synonyms |
Gstt1-1 |
| MMRRC Submission |
044873-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.182)
|
| Stock # |
R6757 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
10 |
| Chromosomal Location |
75619647-75634418 bp(-) (GRCm39) |
| Type of Mutation |
critical splice donor site (2 bp from exon) |
| DNA Base Change (assembly) |
A to T
at 75634217 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000001713
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000001713]
[ENSMUST00000120177]
[ENSMUST00000139724]
|
| AlphaFold |
Q64471 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000001713
|
| SMART Domains |
Protein: ENSMUSP00000001713
Gene: ENSMUSG00000001663
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:GST_N
|
1 |
76 |
7.5e-13 |
PFAM |
|
Pfam:GST_N_3
|
5 |
83 |
1.5e-11 |
PFAM |
|
Pfam:GST_N_2
|
12 |
77 |
1.1e-7 |
PFAM |
|
Pfam:GST_C_3
|
88 |
200 |
6.2e-10 |
PFAM |
|
Pfam:GST_C
|
111 |
201 |
3.7e-10 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000120177
|
| SMART Domains |
Protein: ENSMUSP00000113839
Gene: ENSMUSG00000001663
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:GST_N
|
8 |
68 |
5.8e-11 |
PFAM |
|
Pfam:GST_N_3
|
20 |
77 |
7.3e-9 |
PFAM |
|
Pfam:GST_C
|
103 |
193 |
5e-11 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000139724
|
| SMART Domains |
Protein: ENSMUSP00000117130
Gene: ENSMUSG00000001663
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:GST_N
|
1 |
26 |
4.6e-7 |
PFAM |
|
Pfam:GST_C
|
61 |
126 |
8.1e-7 |
PFAM |
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.6%
- 10x: 98.4%
- 20x: 95.6%
|
| Validation Efficiency |
|
| MGI Phenotype |
PHENOTYPE: Mis homozygous for disruption of this gene appear to be normal and fertile with the exception of a reduced ability to clear 11,2-bis(2-chloroethyl)-1-nitrosourea from the plasma after a single i.p. dose. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 22 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| A830018L16Rik |
A |
T |
1: 11,666,558 (GRCm39) |
*288Y |
probably null |
Het |
| Art2a |
G |
T |
7: 101,204,221 (GRCm39) |
L106I |
probably benign |
Het |
| Bmi1 |
C |
T |
2: 18,688,840 (GRCm39) |
T203M |
probably damaging |
Het |
| Cpm |
A |
G |
10: 117,507,543 (GRCm39) |
D220G |
probably damaging |
Het |
| Cyp2a22 |
A |
C |
7: 26,638,629 (GRCm39) |
D52E |
probably benign |
Het |
| Dag1 |
A |
C |
9: 108,095,216 (GRCm39) |
I92S |
probably damaging |
Het |
| Dntt |
A |
T |
19: 41,025,601 (GRCm39) |
H73L |
probably damaging |
Het |
| Epha5 |
A |
C |
5: 84,253,737 (GRCm39) |
I716S |
probably damaging |
Het |
| Fpr-rs4 |
C |
T |
17: 18,242,394 (GRCm39) |
Q134* |
probably null |
Het |
| Fzd8 |
T |
A |
18: 9,213,238 (GRCm39) |
C107S |
possibly damaging |
Het |
| Garre1 |
G |
A |
7: 33,938,502 (GRCm39) |
A799V |
possibly damaging |
Het |
| Gnptab |
T |
C |
10: 88,273,364 (GRCm39) |
L1047P |
probably damaging |
Het |
| Kdm2a |
T |
C |
19: 4,369,271 (GRCm39) |
R1115G |
probably damaging |
Het |
| Myl10 |
G |
C |
5: 136,726,825 (GRCm39) |
V70L |
probably benign |
Het |
| Myo1b |
C |
T |
1: 51,852,207 (GRCm39) |
E179K |
probably damaging |
Het |
| Nrp1 |
T |
A |
8: 129,152,349 (GRCm39) |
I186N |
probably damaging |
Het |
| Or10g3 |
A |
G |
14: 52,610,172 (GRCm39) |
C113R |
probably damaging |
Het |
| Pole |
T |
C |
5: 110,451,476 (GRCm39) |
V835A |
probably damaging |
Het |
| Shprh |
A |
G |
10: 11,057,252 (GRCm39) |
|
probably null |
Het |
| Slc39a14 |
A |
T |
14: 70,548,333 (GRCm39) |
L238Q |
probably damaging |
Het |
| Spata31e5 |
A |
C |
1: 28,819,191 (GRCm39) |
I30S |
probably damaging |
Het |
| Usp40 |
A |
T |
1: 87,907,759 (GRCm39) |
I619N |
probably damaging |
Het |
|
| Other mutations in Gstt1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01635:Gstt1
|
APN |
10 |
75,629,951 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1460:Gstt1
|
UTSW |
10 |
75,620,004 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1646:Gstt1
|
UTSW |
10 |
75,619,940 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R4603:Gstt1
|
UTSW |
10 |
75,629,969 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7896:Gstt1
|
UTSW |
10 |
75,619,976 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8459:Gstt1
|
UTSW |
10 |
75,629,943 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9792:Gstt1
|
UTSW |
10 |
75,634,391 (GRCm39) |
unclassified |
probably benign |
|
|
R9795:Gstt1
|
UTSW |
10 |
75,634,391 (GRCm39) |
unclassified |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- TCAGTTTCAGCAGGTCCTGG -3'
(R):5'- CACACTTGGCTTGATAGGGATCC -3'
Sequencing Primer
(F):5'- GCCGGCGTCTATTCCACAAAC -3'
(R):5'- TAGGGATCCCTACTAGCCAGC -3'
|
| Posted On |
2018-08-01 |
Incidental Mutation