Mutagenetix > Incidental Mutation

Incidental Mutation 'R1282:Vmn2r28'

151057

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

039348-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R1282 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

5483455-5496850 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 5484301 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 633 (M633K)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably damaging
Transcript: ENSMUST00000086297
AA Change: M633K

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: M633K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.4%
20x: 93.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acmsd	T	C	1: 127,666,297 (GRCm39)	Y23H	probably damaging	Het
Alkbh2	C	T	5: 114,262,287 (GRCm39)	E148K	probably damaging	Het
Atp2a2	G	T	5: 122,629,817 (GRCm39)	T84K	probably benign	Het
Cfap65	T	A	1: 74,964,263 (GRCm39)	T562S	probably benign	Het
Cyp2b10	G	A	7: 25,625,505 (GRCm39)	C436Y	probably damaging	Het
Dnajc7	T	C	11: 100,475,467 (GRCm39)	D381G	probably damaging	Het
Dnase1l3	T	C	14: 7,983,117 (GRCm38)	D129G	probably benign	Het
Espl1	A	G	15: 102,223,826 (GRCm39)	T1126A	probably benign	Het
Exoc3	T	C	13: 74,330,411 (GRCm39)	N506S	probably benign	Het
Fastkd3	C	A	13: 68,732,676 (GRCm39)	N332K	possibly damaging	Het
Fbxw15	G	A	9: 109,387,314 (GRCm39)	S227F	probably damaging	Het
Foxp4	G	A	17: 48,186,568 (GRCm39)	P404S	unknown	Het
Gjb3	G	A	4: 127,220,224 (GRCm39)	R103W	probably damaging	Het
Gm12789	T	A	4: 101,845,487 (GRCm39)	W59R	probably damaging	Het
Greb1l	T	A	18: 10,547,289 (GRCm39)	N1502K	probably benign	Het
H2-K2	A	C	17: 34,218,421 (GRCm39)	I165S	probably damaging	Het
Ipo9	A	G	1: 135,330,030 (GRCm39)	I470T	possibly damaging	Het
Kcnu1	A	T	8: 26,395,985 (GRCm39)	I657F	probably benign	Het
Lrp1b	A	T	2: 40,750,773 (GRCm39)	N2930K	probably damaging	Het
Or56a5	T	A	7: 104,792,859 (GRCm39)	I220F	probably benign	Het
Prag1	T	C	8: 36,567,068 (GRCm39)	V73A	probably damaging	Het
Prrc2b	T	C	2: 32,113,456 (GRCm39)	I1963T	probably damaging	Het
Rep15	C	T	6: 146,934,727 (GRCm39)	R189*	probably null	Het
Resf1	C	T	6: 149,230,670 (GRCm39)	Q1239*	probably null	Het
Rtp3	T	C	9: 110,815,988 (GRCm39)	K188E	probably benign	Het
Scd2	T	C	19: 44,283,620 (GRCm39)	L101P	probably damaging	Het
Scn7a	A	T	2: 66,531,193 (GRCm39)	H561Q	probably damaging	Het
Sfr1	T	C	19: 47,721,407 (GRCm39)	S118P	probably damaging	Het
Slf1	C	T	13: 77,191,959 (GRCm39)	M958I	probably damaging	Het
Snrk	G	T	9: 121,989,586 (GRCm39)	R310L	possibly damaging	Het
Snx11	T	C	11: 96,663,987 (GRCm39)	Y35C	probably damaging	Het
Supt6	T	C	11: 78,119,594 (GRCm39)	T404A	possibly damaging	Het
Svep1	C	A	4: 58,100,032 (GRCm39)	L1337F	possibly damaging	Het
Traf3ip2	C	T	10: 39,502,401 (GRCm39)	T183M	probably damaging	Het
Vmn2r67	A	G	7: 84,785,932 (GRCm39)	V691A	probably benign	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5,491,068 (GRCm39)	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5,491,183 (GRCm39)	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5,489,477 (GRCm39)	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5,484,087 (GRCm39)	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5,489,463 (GRCm39)	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5,491,161 (GRCm39)	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5,484,302 (GRCm39)	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5,491,123 (GRCm39)	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5,493,568 (GRCm39)	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5,484,003 (GRCm39)	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5,487,015 (GRCm39)	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5,491,020 (GRCm39)	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5,496,715 (GRCm39)	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5,491,513 (GRCm39)	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5,493,456 (GRCm39)	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5,491,689 (GRCm39)	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5,484,544 (GRCm39)	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5,496,810 (GRCm39)	missense	probably benign
R1853:Vmn2r28	UTSW	7	5,484,246 (GRCm39)	nonsense	probably null
R1869:Vmn2r28	UTSW	7	5,489,345 (GRCm39)	missense	probably benign	0.00
R1887:Vmn2r28	UTSW	7	5,491,288 (GRCm39)	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5,484,070 (GRCm39)	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5,491,313 (GRCm39)	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5,487,130 (GRCm39)	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5,491,701 (GRCm39)	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5,484,391 (GRCm39)	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5,491,357 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5,493,707 (GRCm39)	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5,483,671 (GRCm39)	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5,489,376 (GRCm39)	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5,490,943 (GRCm39)	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5,487,011 (GRCm39)	missense	possibly damaging	0.61
R5731:Vmn2r28	UTSW	7	5,491,668 (GRCm39)	missense	probably benign	0.41
R6091:Vmn2r28	UTSW	7	5,496,790 (GRCm39)	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5,491,003 (GRCm39)	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5,493,730 (GRCm39)	missense	probably benign	0.01
R6441:Vmn2r28	UTSW	7	5,491,474 (GRCm39)	missense	probably benign	0.00
R6463:Vmn2r28	UTSW	7	5,489,435 (GRCm39)	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5,493,684 (GRCm39)	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5,484,229 (GRCm39)	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5,483,806 (GRCm39)	missense	probably damaging	1.00
R7110:Vmn2r28	UTSW	7	5,493,733 (GRCm39)	missense	probably benign	0.06
R7146:Vmn2r28	UTSW	7	5,484,495 (GRCm39)	missense	probably benign	0.05
R7407:Vmn2r28	UTSW	7	5,484,308 (GRCm39)	missense	probably damaging	1.00
R7563:Vmn2r28	UTSW	7	5,491,200 (GRCm39)	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5,484,255 (GRCm39)	missense	probably benign	0.02
R7808:Vmn2r28	UTSW	7	5,496,678 (GRCm39)	missense	probably damaging	0.98
R7862:Vmn2r28	UTSW	7	5,493,613 (GRCm39)	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5,483,818 (GRCm39)	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5,491,147 (GRCm39)	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5,487,059 (GRCm39)	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5,489,347 (GRCm39)	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5,493,473 (GRCm39)	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5,491,596 (GRCm39)	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5,483,747 (GRCm39)	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5,487,118 (GRCm39)	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5,491,220 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- TGGCTTCATTGAATGCGTCAGGTAG -3'
(R):5'- GCAGAATTGCAAGGCTCCTTTCAAC -3'

Sequencing Primer
(F):5'- ATGCAGTAACTGAGCCCTTG -3'
(R):5'- TTGTCCAGAATACCAGTATGCC -3'

Posted On

2014-01-29