Mutagenetix > Incidental Mutation

Incidental Mutation 'R1475:Zfp874b'

164016

Institutional Source

Beutler Lab

Gene Symbol

Zfp874b

Ensembl Gene

ENSMUSG00000059839

Gene Name

zinc finger protein 874b

Synonyms

9630025I21Rik

MMRRC Submission

039528-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R1475 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

67619632-67632377 bp(-) (GRCm39)

Type of Mutation

splice site (570 bp from exon)

DNA Base Change (assembly)

A to T at 67622211 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000153242 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019572] [ENSMUST00000223868]

AlphaFold

Q7M6X2

Predicted Effect

probably null
Transcript: ENSMUST00000019572
AA Change: C362*

SMART Domains

Protein: ENSMUSP00000019572
Gene: ENSMUSG00000059839
AA Change: C362*

Domain	Start	End	E-Value	Type
KRAB	5	65	4.48e-26	SMART
ZnF_C2H2	136	158	5.9e-3	SMART
ZnF_C2H2	164	186	3.16e-3	SMART
ZnF_C2H2	192	214	6.78e-3	SMART
ZnF_C2H2	220	242	2.4e-3	SMART
ZnF_C2H2	248	268	1.88e2	SMART
ZnF_C2H2	276	298	6.42e-4	SMART
ZnF_C2H2	304	326	2.12e-4	SMART
ZnF_C2H2	332	354	1.28e-3	SMART
ZnF_C2H2	360	382	8.47e-4	SMART
ZnF_C2H2	388	410	1.79e-2	SMART
ZnF_C2H2	416	438	1.47e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000223711

Predicted Effect

probably null
Transcript: ENSMUST00000223868

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000223925

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224308

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225027

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.5%
10x: 96.7%
20x: 93.8%

Validation Efficiency

96% (46/48)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700018B08Rik	A	T	8: 122,267,327 (GRCm39)		probably benign	Het
9230112D13Rik	T	A	14: 34,234,012 (GRCm39)	D93V	unknown	Het
Aatk	G	A	11: 119,901,714 (GRCm39)	T894M	probably damaging	Het
Acacb	T	C	5: 114,333,313 (GRCm39)	I479T	possibly damaging	Het
Acap3	T	C	4: 155,987,278 (GRCm39)	I431T	probably damaging	Het
Adgrl1	A	G	8: 84,664,979 (GRCm39)	K1267R	possibly damaging	Het
Bphl	A	C	13: 34,244,507 (GRCm39)	D208A	probably benign	Het
C2cd5	T	C	6: 143,018,298 (GRCm39)	D308G	possibly damaging	Het
Camsap3	C	T	8: 3,654,708 (GRCm39)	R782C	probably damaging	Het
Cdk11b	G	A	4: 155,718,674 (GRCm39)	R208H	probably damaging	Het
Cfap44	T	C	16: 44,254,175 (GRCm39)		probably benign	Het
Chrna4	T	C	2: 180,671,172 (GRCm39)	S195G	probably benign	Het
Cpsf2	T	C	12: 101,951,495 (GRCm39)	L144S	probably damaging	Het
Creld2	G	A	15: 88,704,834 (GRCm39)	W103*	probably null	Het
Dffa	T	A	4: 149,201,935 (GRCm39)	L171Q	probably damaging	Het
Emcn	C	T	3: 137,085,668 (GRCm39)	H89Y	possibly damaging	Het
Espn	G	A	4: 152,218,728 (GRCm39)	P452S	probably damaging	Het
Fam78b	T	A	1: 166,829,346 (GRCm39)	I71N	probably damaging	Het
Fam89b	A	G	19: 5,779,447 (GRCm39)	S37P	probably damaging	Het
Fat4	G	A	3: 38,942,472 (GRCm39)	R455H	probably damaging	Het
Fbxo6	A	G	4: 148,230,567 (GRCm39)	F232L	probably benign	Het
Fcamr	T	A	1: 130,742,221 (GRCm39)		probably null	Het
Fermt3	T	C	19: 6,996,242 (GRCm39)		probably null	Het
Fsip2	A	T	2: 82,817,539 (GRCm39)	D4424V	probably damaging	Het
Gaa	G	A	11: 119,165,142 (GRCm39)		probably null	Het
Glce	T	C	9: 61,968,210 (GRCm39)	T314A	possibly damaging	Het
Hdac5	T	C	11: 102,093,012 (GRCm39)	Q575R	possibly damaging	Het
Il23r	C	A	6: 67,429,280 (GRCm39)		probably null	Het
Kcnj4	T	A	15: 79,368,831 (GRCm39)	E383V	probably damaging	Het
Lrsam1	G	T	2: 32,844,277 (GRCm39)	Q115K	possibly damaging	Het
Lyst	T	A	13: 13,882,797 (GRCm39)		probably null	Het
Myf5	A	G	10: 107,320,515 (GRCm39)	V190A	probably benign	Het
Nmnat2	G	A	1: 152,950,441 (GRCm39)	R42H	probably damaging	Het
Or8b42	T	A	9: 38,342,160 (GRCm39)	V194D	probably benign	Het
Or8k20	A	T	2: 86,105,905 (GRCm39)	*309R	probably null	Het
Osbpl1a	T	A	18: 12,890,737 (GRCm39)	K380M	probably damaging	Het
Pgd	T	C	4: 149,241,232 (GRCm39)	T226A	probably benign	Het
Pitpnm3	T	C	11: 71,965,453 (GRCm39)	T127A	probably damaging	Het
Plekhm2	T	C	4: 141,355,165 (GRCm39)	D954G	possibly damaging	Het
Pramel14	T	C	4: 143,720,882 (GRCm39)	K20E	probably benign	Het
Rasal1	T	A	5: 120,801,047 (GRCm39)	F236I	possibly damaging	Het
Stab1	T	C	14: 30,885,785 (GRCm39)	N63S	probably benign	Het
Syf2	T	A	4: 134,662,745 (GRCm39)	M145K	possibly damaging	Het
Usp34	C	A	11: 23,423,253 (GRCm39)	L3152I	probably damaging	Het
Usp50	T	C	2: 126,611,787 (GRCm39)		probably null	Het
Wdfy4	A	T	14: 32,830,645 (GRCm39)	I929N	probably benign	Het

Other mutations in Zfp874b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02172:Zfp874b	APN	13	67,622,135 (GRCm39)	missense	probably damaging	1.00
R0432:Zfp874b	UTSW	13	67,629,955 (GRCm39)	missense	probably damaging	1.00
R0653:Zfp874b	UTSW	13	67,623,052 (GRCm39)	missense	possibly damaging	0.69
R0727:Zfp874b	UTSW	13	67,622,831 (GRCm39)	missense	probably damaging	1.00
R1726:Zfp874b	UTSW	13	67,622,839 (GRCm39)	missense	probably damaging	1.00
R5484:Zfp874b	UTSW	13	67,629,373 (GRCm39)	missense	possibly damaging	0.76
R6386:Zfp874b	UTSW	13	67,622,962 (GRCm39)	missense	possibly damaging	0.70
R7029:Zfp874b	UTSW	13	67,622,392 (GRCm39)	missense	probably damaging	0.98
R7150:Zfp874b	UTSW	13	67,622,622 (GRCm39)	nonsense	probably null
R7726:Zfp874b	UTSW	13	67,621,975 (GRCm39)	missense	probably benign	0.01
R7778:Zfp874b	UTSW	13	67,622,093 (GRCm39)	missense	probably benign	0.03
R7824:Zfp874b	UTSW	13	67,622,093 (GRCm39)	missense	probably benign	0.03
R7918:Zfp874b	UTSW	13	67,622,279 (GRCm39)	missense	possibly damaging	0.74
R7939:Zfp874b	UTSW	13	67,622,622 (GRCm39)	nonsense	probably null
R8053:Zfp874b	UTSW	13	67,622,217 (GRCm39)	missense	probably damaging	0.96
R8881:Zfp874b	UTSW	13	67,622,141 (GRCm39)	missense	probably damaging	0.99
X0028:Zfp874b	UTSW	13	67,622,179 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- GCTTTGTGACAGTCTTCGCATTGATATG -3'
(R):5'- GGTACAAGTGTCAAGAATGTGGCAAATC -3'

Sequencing Primer
(F):5'- ttcccacactcttcacacg -3'
(R):5'- agtatccctacaagtgcgaag -3'

Posted On

2014-03-28