Mutagenetix > Incidental Mutation

Incidental Mutation 'R4009:Zfp618'

311593

Institutional Source

Beutler Lab

Gene Symbol

Zfp618

Ensembl Gene

ENSMUSG00000028358

Gene Name

zinc finger protein 618

Synonyms

Nedd10, 2810040O04Rik, D430033D05Rik, 2810031P15Rik

MMRRC Submission

040846-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.129) question?

Stock #

R4009 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

62883810-63057945 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 63051801 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 861 (A861T)

Ref Sequence

ENSEMBL: ENSMUSP00000103038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064814] [ENSMUST00000107415]

AlphaFold

Q80YY7

Predicted Effect

probably benign
Transcript: ENSMUST00000064814
AA Change: A768T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000069275
Gene: ENSMUSG00000028358
AA Change: A768T

Domain	Start	End	E-Value	Type
ZnF_C2H2	114	136	5.06e-2	SMART
ZnF_C2H2	155	177	8.81e-2	SMART
ZnF_C2H2	243	265	2.91e-2	SMART
low complexity region	288	295	N/A	INTRINSIC
ZnF_C2H2	298	320	2.53e-2	SMART
PDB:2BW3\|A	377	690	5e-8	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000107415
AA Change: A861T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000103038
Gene: ENSMUSG00000028358
AA Change: A861T

Domain	Start	End	E-Value	Type
ZnF_C2H2	146	168	5.06e-2	SMART
ZnF_C2H2	187	209	8.81e-2	SMART
ZnF_C2H2	255	277	2.91e-2	SMART
low complexity region	381	388	N/A	INTRINSIC
ZnF_C2H2	391	413	2.53e-2	SMART
PDB:2BW3\|A	479	783	9e-8	PDB

Meta Mutation Damage Score

0.0723

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 93.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	A	T	11: 109,980,933 (GRCm39)	L35Q	probably damaging	Het
Acss2	T	A	2: 155,399,548 (GRCm39)	L529Q	probably damaging	Het
Apc2	T	A	10: 80,149,426 (GRCm39)	D1464E	probably benign	Het
Atp8a2	A	G	14: 60,265,434 (GRCm39)	S301P	possibly damaging	Het
Cntln	A	G	4: 84,981,452 (GRCm39)	T877A	probably benign	Het
Cntn6	T	C	6: 104,810,783 (GRCm39)	I537T	probably damaging	Het
Cyp2d12	T	G	15: 82,440,493 (GRCm39)	L94R	probably damaging	Het
Dnah7a	A	G	1: 53,564,164 (GRCm39)	L1965P	probably damaging	Het
Dock10	T	C	1: 80,510,148 (GRCm39)	N1560S	probably damaging	Het
F830045P16Rik	A	T	2: 129,305,467 (GRCm39)	N302K	probably damaging	Het
Fxr1	G	T	3: 34,119,171 (GRCm39)	R580L	probably benign	Het
Gm5592	G	T	7: 40,938,934 (GRCm39)	V739L	probably benign	Het
Gna13	A	G	11: 109,286,843 (GRCm39)	D222G	probably damaging	Het
Grm7	T	A	6: 111,472,683 (GRCm39)	Y841N	probably damaging	Het
Kat2b	G	A	17: 53,951,769 (GRCm39)		probably null	Het
Kcng4	A	G	8: 120,352,824 (GRCm39)	V362A	probably damaging	Het
Kcnh1	T	A	1: 191,959,448 (GRCm39)	I334N	probably benign	Het
Mapkbp1	T	C	2: 119,854,086 (GRCm39)	S1222P	probably benign	Het
Or10a3m	A	T	7: 108,313,366 (GRCm39)	I269L	probably benign	Het
Or10ag57	A	T	2: 87,218,924 (GRCm39)	I292F	possibly damaging	Het
Or11g26	T	C	14: 50,753,419 (GRCm39)	S253P	possibly damaging	Het
Or5h24	G	A	16: 58,919,124 (GRCm39)	T77I	unknown	Het
Pik3cb	A	G	9: 98,922,982 (GRCm39)	Y1017H	probably damaging	Het
Plpp5	A	G	8: 26,210,338 (GRCm39)	E36G	probably damaging	Het
Pnma8a	A	T	7: 16,695,301 (GRCm39)	K385N	probably damaging	Het
Ptprd	T	G	4: 75,874,634 (GRCm39)	M1272L	possibly damaging	Het
Rad51ap2	A	G	12: 11,507,052 (GRCm39)	I325V	probably benign	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Skint4	T	C	4: 111,977,306 (GRCm39)	V232A	possibly damaging	Het
Slc30a5	G	A	13: 100,945,741 (GRCm39)	A537V	probably damaging	Het
Tcerg1	CAATTGAAAA	CAA	18: 42,697,201 (GRCm39)		probably null	Het
Tenm3	C	A	8: 48,802,258 (GRCm39)	K162N	probably damaging	Het
Vmn2r44	T	A	7: 8,380,987 (GRCm39)	Q302L	possibly damaging	Het

Other mutations in Zfp618

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01301:Zfp618	APN	4	63,051,063 (GRCm39)	missense	probably damaging	1.00
IGL01563:Zfp618	APN	4	62,998,133 (GRCm39)	missense	probably benign	0.38
IGL01726:Zfp618	APN	4	63,050,872 (GRCm39)	missense	probably damaging	1.00
IGL02139:Zfp618	APN	4	63,051,773 (GRCm39)	missense	probably damaging	1.00
IGL02182:Zfp618	APN	4	63,013,798 (GRCm39)	splice site	probably benign
IGL02533:Zfp618	APN	4	63,007,642 (GRCm39)	missense	probably damaging	1.00
IGL03231:Zfp618	APN	4	63,012,716 (GRCm39)	missense	probably damaging	1.00
IGL03257:Zfp618	APN	4	63,050,908 (GRCm39)	missense	probably damaging	1.00
ANU18:Zfp618	UTSW	4	63,051,063 (GRCm39)	missense	probably damaging	1.00
IGL03014:Zfp618	UTSW	4	62,998,325 (GRCm39)	missense	probably damaging	1.00
R0288:Zfp618	UTSW	4	63,051,171 (GRCm39)	missense	possibly damaging	0.57
R0408:Zfp618	UTSW	4	63,004,809 (GRCm39)	missense	probably damaging	0.97
R0685:Zfp618	UTSW	4	63,052,011 (GRCm39)	missense	probably benign	0.21
R1482:Zfp618	UTSW	4	63,033,685 (GRCm39)	missense	possibly damaging	0.64
R1585:Zfp618	UTSW	4	63,051,175 (GRCm39)	missense	probably damaging	1.00
R1649:Zfp618	UTSW	4	63,013,774 (GRCm39)	missense	probably damaging	1.00
R1744:Zfp618	UTSW	4	63,004,871 (GRCm39)	splice site	probably benign
R1793:Zfp618	UTSW	4	63,051,474 (GRCm39)	missense	probably damaging	0.97
R1952:Zfp618	UTSW	4	63,050,555 (GRCm39)	splice site	probably null
R1996:Zfp618	UTSW	4	63,049,452 (GRCm39)	splice site	probably null
R3792:Zfp618	UTSW	4	63,033,728 (GRCm39)	intron	probably benign
R3803:Zfp618	UTSW	4	63,051,256 (GRCm39)	missense	probably damaging	1.00
R3821:Zfp618	UTSW	4	63,051,801 (GRCm39)	missense	probably benign	0.00
R3838:Zfp618	UTSW	4	63,051,801 (GRCm39)	missense	probably benign	0.00
R4010:Zfp618	UTSW	4	63,051,801 (GRCm39)	missense	probably benign	0.00
R4565:Zfp618	UTSW	4	63,039,588 (GRCm39)	missense	probably damaging	1.00
R4611:Zfp618	UTSW	4	63,051,216 (GRCm39)	missense	probably damaging	1.00
R5019:Zfp618	UTSW	4	63,021,789 (GRCm39)	missense	probably damaging	1.00
R5154:Zfp618	UTSW	4	63,051,446 (GRCm39)	missense	probably damaging	1.00
R5183:Zfp618	UTSW	4	63,017,519 (GRCm39)	missense	probably benign
R5354:Zfp618	UTSW	4	62,998,265 (GRCm39)	missense	probably damaging	1.00
R5383:Zfp618	UTSW	4	63,013,729 (GRCm39)	missense	probably benign	0.33
R5774:Zfp618	UTSW	4	63,050,799 (GRCm39)	missense	probably damaging	1.00
R5932:Zfp618	UTSW	4	63,036,803 (GRCm39)	nonsense	probably null
R6101:Zfp618	UTSW	4	63,051,478 (GRCm39)	missense	probably benign	0.09
R6105:Zfp618	UTSW	4	63,051,478 (GRCm39)	missense	probably benign	0.09
R6478:Zfp618	UTSW	4	63,050,943 (GRCm39)	missense	probably damaging	1.00
R6598:Zfp618	UTSW	4	63,007,636 (GRCm39)	missense	probably damaging	1.00
R7386:Zfp618	UTSW	4	63,013,622 (GRCm39)	critical splice donor site	probably null
R7666:Zfp618	UTSW	4	63,050,954 (GRCm39)	nonsense	probably null
R7678:Zfp618	UTSW	4	63,004,858 (GRCm39)	missense	probably benign	0.07
R7975:Zfp618	UTSW	4	63,049,352 (GRCm39)	missense	possibly damaging	0.93
R8276:Zfp618	UTSW	4	63,051,193 (GRCm39)	missense	probably damaging	1.00
R8421:Zfp618	UTSW	4	63,051,483 (GRCm39)	missense	probably damaging	1.00
R8988:Zfp618	UTSW	4	63,012,708 (GRCm39)	missense	probably benign	0.09
R9022:Zfp618	UTSW	4	63,012,687 (GRCm39)	missense	probably damaging	1.00
R9150:Zfp618	UTSW	4	63,039,603 (GRCm39)	nonsense	probably null
R9163:Zfp618	UTSW	4	63,051,511 (GRCm39)	missense	probably damaging	1.00
R9364:Zfp618	UTSW	4	63,036,824 (GRCm39)	missense	probably damaging	0.99
R9382:Zfp618	UTSW	4	63,051,258 (GRCm39)	missense	probably damaging	0.97
R9424:Zfp618	UTSW	4	63,051,282 (GRCm39)	missense	probably benign	0.00
R9462:Zfp618	UTSW	4	63,051,510 (GRCm39)	missense	possibly damaging	0.94
R9576:Zfp618	UTSW	4	63,051,282 (GRCm39)	missense	probably benign	0.00
R9587:Zfp618	UTSW	4	63,051,916 (GRCm39)	missense
X0011:Zfp618	UTSW	4	62,998,243 (GRCm39)	missense	probably damaging	0.99
Z1176:Zfp618	UTSW	4	63,051,000 (GRCm39)	missense	probably benign	0.12
Z1176:Zfp618	UTSW	4	63,013,734 (GRCm39)	missense	probably benign	0.29

Predicted Primers

PCR Primer
(F):5'- CGCTCAAGGAGAACTTCAAGG -3'
(R):5'- ATATTTACACACCCGCTCCG -3'

Sequencing Primer
(F):5'- GGAGAACTTCAAGGTGCACCC -3'
(R):5'- GAGCCCCGACAGCAGGAAC -3'

Posted On

2015-04-29