Mutagenetix > Incidental Mutation

Incidental Mutation 'R4159:Vmn2r3'

315650

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

MMRRC Submission

041002-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4159 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64166225-64197130 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 64194850 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 23 (Q23*)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

probably benign
Transcript: ENSMUST00000170244

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000176328
AA Change: Q23*

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: Q23*

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.6%

Validation Efficiency

96% (45/47)

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam4	T	G	12: 81,466,806 (GRCm39)	D605A	probably damaging	Het
Adcy1	A	G	11: 7,013,889 (GRCm39)	H97R	probably damaging	Het
Amdhd1	A	G	10: 93,370,512 (GRCm39)	Y109H	probably damaging	Het
Ankhd1	A	G	18: 36,722,593 (GRCm39)	N372S	possibly damaging	Het
Aoc2	G	A	11: 101,216,122 (GRCm39)	M68I	probably damaging	Het
Aspscr1	G	A	11: 120,599,502 (GRCm39)	A377T	probably damaging	Het
Bltp1	T	A	3: 36,985,232 (GRCm39)	M854K	probably benign	Het
Bltp3a	A	G	17: 28,103,061 (GRCm39)	Y365C	probably damaging	Het
Cacnb1	G	C	11: 97,903,100 (GRCm39)	C154W	probably damaging	Het
Ccdc186	G	A	19: 56,781,924 (GRCm39)	R27*	probably null	Het
Clnk	C	A	5: 38,899,138 (GRCm39)		probably benign	Het
Dse	A	G	10: 34,029,330 (GRCm39)	F587L	probably damaging	Het
Fut8	T	C	12: 77,440,523 (GRCm39)	L170P	probably damaging	Het
G3bp2	T	C	5: 92,212,260 (GRCm39)	H217R	probably benign	Het
Gm19345	G	A	7: 19,588,886 (GRCm39)		probably benign	Het
Hjurp	G	C	1: 88,204,937 (GRCm39)		probably benign	Het
Kcne4	A	G	1: 78,795,819 (GRCm39)	N156D	probably benign	Het
Met	G	T	6: 17,562,271 (GRCm39)		probably null	Het
Mfsd3	T	C	15: 76,585,945 (GRCm39)	L26P	probably damaging	Het
Oasl1	A	G	5: 115,075,073 (GRCm39)	K378E	possibly damaging	Het
Pde8a	A	G	7: 80,970,407 (GRCm39)	I510V	probably benign	Het
Pds5a	T	C	5: 65,821,839 (GRCm39)	T120A	possibly damaging	Het
Phf8-ps	G	A	17: 33,285,023 (GRCm39)	T593I	probably benign	Het
Phkb	T	A	8: 86,748,162 (GRCm39)		probably null	Het
Ptprz1	A	T	6: 23,001,683 (GRCm39)	K1258*	probably null	Het
Senp7	C	A	16: 55,973,832 (GRCm39)	P351Q	possibly damaging	Het
Slc15a5	G	T	6: 138,049,938 (GRCm39)	T159K	possibly damaging	Het
Slc39a6	A	G	18: 24,730,885 (GRCm39)	V362A	possibly damaging	Het
Spef2	A	T	15: 9,676,407 (GRCm39)	D721E	probably damaging	Het
Sting1	A	T	18: 35,872,272 (GRCm39)	Y77N	probably damaging	Het
Tex11	C	A	X: 99,977,021 (GRCm39)	A487S	possibly damaging	Het
Tnxb	A	G	17: 34,930,491 (GRCm39)	T2059A	probably damaging	Het
Ttll8	T	C	15: 88,801,444 (GRCm39)	N415D	probably benign	Het
Ube2d2a	A	T	18: 35,903,577 (GRCm39)		probably benign	Het
Unc79	A	G	12: 103,036,512 (GRCm39)		probably benign	Het
Ush2a	G	A	1: 188,460,907 (GRCm39)	V2723M	probably damaging	Het
Vmn2r102	T	A	17: 19,898,088 (GRCm39)	C368S	probably damaging	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64,167,304 (GRCm39)	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64,182,382 (GRCm39)	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64,182,476 (GRCm39)	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64,178,620 (GRCm39)	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64,194,816 (GRCm39)	missense	probably benign
IGL02719:Vmn2r3	APN	3	64,183,031 (GRCm39)	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64,167,239 (GRCm39)	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64,186,256 (GRCm39)	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64,182,767 (GRCm39)	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64,182,787 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64,183,054 (GRCm39)	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64,182,697 (GRCm39)	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64,182,538 (GRCm39)	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64,194,810 (GRCm39)	missense	possibly damaging	0.73
R1806:Vmn2r3	UTSW	3	64,182,893 (GRCm39)	missense	probably benign	0.03
R1852:Vmn2r3	UTSW	3	64,166,815 (GRCm39)	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64,166,537 (GRCm39)	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64,182,493 (GRCm39)	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64,166,483 (GRCm39)	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64,182,733 (GRCm39)	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64,183,138 (GRCm39)	missense	probably damaging	0.99
R4494:Vmn2r3	UTSW	3	64,182,692 (GRCm39)	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64,183,022 (GRCm39)	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64,167,182 (GRCm39)	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64,166,618 (GRCm39)	missense	probably damaging	1.00
R5018:Vmn2r3	UTSW	3	64,178,774 (GRCm39)	missense	probably benign
R5033:Vmn2r3	UTSW	3	64,167,220 (GRCm39)	missense	probably benign	0.09
R5126:Vmn2r3	UTSW	3	64,166,740 (GRCm39)	missense	probably damaging	1.00
R5148:Vmn2r3	UTSW	3	64,186,247 (GRCm39)	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64,166,978 (GRCm39)	nonsense	probably null
R5785:Vmn2r3	UTSW	3	64,166,444 (GRCm39)	missense	possibly damaging	0.89
R5905:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R5992:Vmn2r3	UTSW	3	64,167,068 (GRCm39)	missense	probably damaging	1.00
R6028:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64,186,182 (GRCm39)	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64,182,517 (GRCm39)	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64,183,039 (GRCm39)	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64,182,327 (GRCm39)	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64,166,927 (GRCm39)	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64,166,668 (GRCm39)	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64,194,732 (GRCm39)	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64,167,364 (GRCm39)	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64,168,825 (GRCm39)	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64,182,953 (GRCm39)	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64,182,770 (GRCm39)	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64,182,939 (GRCm39)	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64,186,235 (GRCm39)	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64,182,871 (GRCm39)	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64,178,714 (GRCm39)	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64,182,347 (GRCm39)	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64,178,617 (GRCm39)	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64,186,370 (GRCm39)	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64,182,311 (GRCm39)	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64,166,896 (GRCm39)	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64,182,383 (GRCm39)	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64,194,892 (GRCm39)	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64,166,695 (GRCm39)	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64,166,673 (GRCm39)	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64,168,803 (GRCm39)	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64,186,403 (GRCm39)	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64,178,735 (GRCm39)	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64,178,724 (GRCm39)	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64,182,389 (GRCm39)	missense	probably damaging	1.00
X0022:Vmn2r3	UTSW	3	64,178,669 (GRCm39)	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- CTAGGCTCCAAGGTATCCACTC -3'
(R):5'- GCAATCAGGTTAGGCACTCAC -3'

Sequencing Primer
(F):5'- AAGGTATCCACTCTTTTCTGGC -3'
(R):5'- GGTTAGGCACTCACACACTC -3'

Posted On

2015-05-14