Mutagenetix > Incidental Mutation

Incidental Mutation 'R0409:Or5m9b'

36499

Institutional Source

Beutler Lab

Gene Symbol

Or5m9b

Ensembl Gene

ENSMUSG00000099820

Gene Name

olfactory receptor family 5 subfamily M member 9B

Synonyms

Olfr1036, GA_x6K02T2Q125-47549689-47550621, MOR262-13, MOR245-25

MMRRC Submission

038611-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.284) question?

Stock #

R0409 (G1)

Quality Score

183

Status

Validated

Chromosome

Chromosomal Location

85905048-85906062 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 85905646 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 187 (C187*)

Ref Sequence

ENSEMBL: ENSMUSP00000130843 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164985]

AlphaFold

Q7TR85

Predicted Effect

probably null
Transcript: ENSMUST00000164985
AA Change: C187*

SMART Domains

Protein: ENSMUSP00000130843
Gene: ENSMUSG00000099820
AA Change: C187*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	2.2e-51	PFAM
Pfam:7tm_1	39	288	1.1e-21	PFAM

Meta Mutation Damage Score

0.9661

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.9%
20x: 94.8%

Validation Efficiency

100% (55/55)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	G	T	10: 87,061,816 (GRCm39)	A89S	probably damaging	Het
Alkbh3	T	A	2: 93,831,793 (GRCm39)	I146F	possibly damaging	Het
Aox1	A	T	1: 58,375,783 (GRCm39)	I871F	possibly damaging	Het
Birc2	A	C	9: 7,819,385 (GRCm39)	V509G	possibly damaging	Het
Car7	G	A	8: 105,275,056 (GRCm39)	A165T	probably damaging	Het
Ccdc81	A	G	7: 89,535,423 (GRCm39)	V271A	probably benign	Het
Cdc40	G	T	10: 40,723,164 (GRCm39)	H302N	probably damaging	Het
Cep104	C	T	4: 154,067,510 (GRCm39)		probably benign	Het
Cfap54	C	A	10: 92,612,075 (GRCm39)	S3161I	probably benign	Het
Chil5	A	G	3: 105,942,282 (GRCm39)		probably benign	Het
Chil6	C	T	3: 106,311,492 (GRCm39)	G96D	probably benign	Het
Cnot1	T	C	8: 96,475,483 (GRCm39)	K531E	probably damaging	Het
Colgalt2	G	T	1: 152,384,312 (GRCm39)	A551S	possibly damaging	Het
Disp3	T	G	4: 148,356,416 (GRCm39)	E148A	probably damaging	Het
Eps8l2	A	G	7: 140,922,893 (GRCm39)	Y52C	probably damaging	Het
Exph5	C	A	9: 53,285,643 (GRCm39)	T908K	probably benign	Het
Fat4	C	T	3: 39,031,562 (GRCm39)	S2449F	probably damaging	Het
Faxc	T	A	4: 21,948,751 (GRCm39)	N154K	probably benign	Het
Fbxo43	C	T	15: 36,162,503 (GRCm39)	A235T	probably benign	Het
Fnip1	A	G	11: 54,371,180 (GRCm39)		probably null	Het
Fsd1l	T	C	4: 53,679,932 (GRCm39)	L210P	probably benign	Het
Gm6420	A	C	1: 23,295,119 (GRCm39)	S123R	unknown	Het
Gm8801	T	G	17: 36,258,268 (GRCm39)		noncoding transcript	Het
Gmfb	T	C	14: 47,053,679 (GRCm39)	I36V	probably benign	Het
Gsap	G	A	5: 21,427,443 (GRCm39)		probably benign	Het
Hectd1	T	A	12: 51,829,339 (GRCm39)	I969L	possibly damaging	Het
Il21r	G	T	7: 125,229,012 (GRCm39)		probably benign	Het
Lrrc7	A	G	3: 157,867,063 (GRCm39)	F893L	possibly damaging	Het
Map2	A	G	1: 66,472,739 (GRCm39)	I1715V	probably damaging	Het
Mlh3	A	G	12: 85,287,628 (GRCm39)	I1339T	possibly damaging	Het
Nacad	T	C	11: 6,549,810 (GRCm39)	D1127G	probably benign	Het
Noc3l	A	G	19: 38,806,371 (GRCm39)		probably benign	Het
Nup93	A	G	8: 95,030,293 (GRCm39)	D384G	probably damaging	Het
Or5p54	T	C	7: 107,554,433 (GRCm39)	I195T	probably benign	Het
Or8b40	C	T	9: 38,027,547 (GRCm39)	L152F	probably benign	Het
Pls1	A	T	9: 95,668,972 (GRCm39)		probably benign	Het
Prkcb	A	T	7: 122,024,200 (GRCm39)	H75L	probably damaging	Het
Rev1	A	T	1: 38,113,449 (GRCm39)	Y539*	probably null	Het
Rnf10	A	T	5: 115,393,506 (GRCm39)		probably benign	Het
Rnpepl1	A	G	1: 92,843,582 (GRCm39)	Y234C	probably damaging	Het
Sdk2	T	C	11: 113,741,717 (GRCm39)		probably benign	Het
Sec23b	T	A	2: 144,409,832 (GRCm39)	M240K	probably benign	Het
Sema5a	A	T	15: 32,681,755 (GRCm39)	N945Y	probably damaging	Het
Snapc4	C	A	2: 26,257,228 (GRCm39)	R799L	probably benign	Het
Spata31g1	A	C	4: 42,972,203 (GRCm39)	K512T	probably damaging	Het
Tctn3	T	C	19: 40,599,860 (GRCm39)		probably benign	Het
Tex56	A	T	13: 35,108,532 (GRCm39)	I5L	probably benign	Het
Tfpt	G	A	7: 3,623,898 (GRCm39)	Q50*	probably null	Het
Trim80	T	C	11: 115,332,039 (GRCm39)	V77A	probably damaging	Het
Trp73	T	A	4: 154,148,841 (GRCm39)	D256V	possibly damaging	Het
Utrn	G	T	10: 12,519,345 (GRCm39)	N2202K	probably benign	Het
Vps13c	T	A	9: 67,858,926 (GRCm39)	F2792Y	probably benign	Het

Other mutations in Or5m9b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02260:Or5m9b	APN	2	85,905,816 (GRCm39)	missense	possibly damaging	0.90
IGL02370:Or5m9b	APN	2	85,905,132 (GRCm39)	missense	probably damaging	0.98
R0269:Or5m9b	UTSW	2	85,905,485 (GRCm39)	missense	probably benign	0.03
R0412:Or5m9b	UTSW	2	85,905,435 (GRCm39)	missense	probably benign	0.16
R0617:Or5m9b	UTSW	2	85,905,485 (GRCm39)	missense	probably benign	0.03
R0846:Or5m9b	UTSW	2	85,905,510 (GRCm39)	missense	possibly damaging	0.66
R1251:Or5m9b	UTSW	2	85,905,164 (GRCm39)	missense	probably benign	0.20
R1646:Or5m9b	UTSW	2	85,905,960 (GRCm39)	missense	probably damaging	0.98
R1670:Or5m9b	UTSW	2	85,905,594 (GRCm39)	missense	probably benign
R1775:Or5m9b	UTSW	2	85,905,104 (GRCm39)	missense	possibly damaging	0.88
R1968:Or5m9b	UTSW	2	85,905,549 (GRCm39)	missense	probably damaging	1.00
R2877:Or5m9b	UTSW	2	85,905,675 (GRCm39)	missense	possibly damaging	0.82
R4639:Or5m9b	UTSW	2	85,905,923 (GRCm39)	missense	probably benign	0.24
R4957:Or5m9b	UTSW	2	85,905,854 (GRCm39)	missense	probably damaging	1.00
R5267:Or5m9b	UTSW	2	85,905,882 (GRCm39)	missense	probably benign	0.28
R5862:Or5m9b	UTSW	2	85,905,990 (GRCm39)	missense	probably benign	0.00
R6235:Or5m9b	UTSW	2	85,905,510 (GRCm39)	missense	possibly damaging	0.66
R6355:Or5m9b	UTSW	2	85,905,216 (GRCm39)	missense	probably benign	0.04
R6943:Or5m9b	UTSW	2	85,905,264 (GRCm39)	missense	probably damaging	1.00
R8033:Or5m9b	UTSW	2	85,905,219 (GRCm39)	missense	possibly damaging	0.61
R8796:Or5m9b	UTSW	2	85,905,518 (GRCm39)	missense	possibly damaging	0.94
R8861:Or5m9b	UTSW	2	85,905,960 (GRCm39)	missense	probably damaging	0.98
Z1088:Or5m9b	UTSW	2	85,905,667 (GRCm39)	missense	possibly damaging	0.66
Z1177:Or5m9b	UTSW	2	85,905,768 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATGTCCAGGGTTGTCTGTATCCGC -3'
(R):5'- GGGGTTCAGCATAGGAATCACACTG -3'

Sequencing Primer
(F):5'- TGTATCCGCCTCATTTCTGTG -3'
(R):5'- CTCAGATACATGAATATGAGGGTTCC -3'

Posted On

2013-05-09