Mutagenetix > Incidental Mutation

Incidental Mutation 'R4882:Irf9'

375351

Institutional Source

Beutler Lab

Gene Symbol

Irf9

Ensembl Gene

ENSMUSG00000002325

Gene Name

interferon regulatory factor 9

Synonyms

p48, Isgf3g, Irf-9

MMRRC Submission

042490-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.210) question?

Stock #

R4882 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

55841442-55847487 bp(+) (GRCm39)

Type of Mutation

utr 3 prime

DNA Base Change (assembly)

G to T at 55846496 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000130697] [ENSMUST00000134863] [ENSMUST00000138037]

AlphaFold

Q61179

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126269

Predicted Effect

probably benign
Transcript: ENSMUST00000130697

SMART Domains

Protein: ENSMUSP00000120359
Gene: ENSMUSG00000002325

Domain	Start	End	E-Value	Type
IRF	5	117	1.19e-53	SMART
low complexity region	158	182	N/A	INTRINSIC
low complexity region	185	194	N/A	INTRINSIC
IRF-3	211	377	1.13e-59	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000134863

SMART Domains

Protein: ENSMUSP00000120525
Gene: ENSMUSG00000002325

Domain	Start	End	E-Value	Type
low complexity region	34	58	N/A	INTRINSIC
IRF	71	183	1.19e-53	SMART
low complexity region	224	248	N/A	INTRINSIC
low complexity region	251	260	N/A	INTRINSIC
IRF-3	277	443	1.13e-59	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136109

Predicted Effect

probably benign
Transcript: ENSMUST00000138037

SMART Domains

Protein: ENSMUSP00000119477
Gene: ENSMUSG00000002325

Domain	Start	End	E-Value	Type
IRF	23	135	1.19e-53	SMART
low complexity region	176	200	N/A	INTRINSIC
low complexity region	203	212	N/A	INTRINSIC
IRF-3	229	395	1.13e-59	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145680

Predicted Effect

probably benign
Transcript: ENSMUST00000226275

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 96.8%
20x: 93.9%

Validation Efficiency

98% (59/60)

MGI Phenotype

PHENOTYPE: Mice homozygous for disruptions in this gene display an apparently normal phenotype. However, antivirus response induced by Ifn alfpha and Ifn gamma are impaired. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acap3	A	G	4: 155,990,112 (GRCm39)	D764G	probably damaging	Het
Adgrg6	T	A	10: 14,310,081 (GRCm39)	I775F	possibly damaging	Het
Ahnak	T	A	19: 8,983,261 (GRCm39)	M1515K	probably damaging	Het
Aqp4	A	G	18: 15,531,311 (GRCm39)	V150A	possibly damaging	Het
Axdnd1	A	T	1: 156,223,129 (GRCm39)		probably null	Het
Bap1	C	T	14: 30,973,678 (GRCm39)		probably benign	Het
BB014433	A	G	8: 15,092,016 (GRCm39)	V279A	probably benign	Het
Cacng8	T	C	7: 3,460,669 (GRCm39)	Y151H	probably damaging	Het
Caskin1	T	C	17: 24,723,389 (GRCm39)	S726P	probably damaging	Het
Cd200	T	C	16: 45,217,380 (GRCm39)	T104A	probably benign	Het
Cdk12	C	T	11: 98,101,272 (GRCm39)	R377C	unknown	Het
Ceacam13	A	G	7: 17,746,997 (GRCm39)	H150R	probably benign	Het
Cebpzos	T	C	17: 79,227,220 (GRCm39)	Y65H	probably benign	Het
Cgnl1	T	A	9: 71,624,683 (GRCm39)	M630L	probably benign	Het
Dop1b	G	T	16: 93,549,802 (GRCm39)	R247L	possibly damaging	Het
Dqx1	A	G	6: 83,043,069 (GRCm39)		probably null	Het
Etaa1	T	C	11: 17,896,174 (GRCm39)	S648G	probably benign	Het
Flnb	A	G	14: 7,929,936 (GRCm38)	D2022G	possibly damaging	Het
Gpr158	T	A	2: 21,830,059 (GRCm39)	N701K	probably damaging	Het
H2-Eb2	C	T	17: 34,553,230 (GRCm39)	H139Y	probably benign	Het
Hbb-bh2	A	T	7: 103,488,455 (GRCm39)	V114E	probably damaging	Het
Ifna9	T	A	4: 88,510,540 (GRCm39)	Q28L	probably benign	Het
Inca1	T	C	11: 70,579,566 (GRCm39)	T188A	probably benign	Het
Kdm1b	T	A	13: 47,214,369 (GRCm39)	H238Q	probably benign	Het
Lpin1	G	C	12: 16,588,537 (GRCm39)	F851L	probably damaging	Het
Map3k9	C	T	12: 81,770,936 (GRCm39)	R884Q	probably damaging	Het
Mcm3ap	C	T	10: 76,320,495 (GRCm39)	Q818*	probably null	Het
Mcm7	A	T	5: 138,164,173 (GRCm39)		probably null	Het
Npdc1	G	A	2: 25,298,957 (GRCm39)	D284N	probably damaging	Het
Nppa	G	T	4: 148,085,544 (GRCm39)	M50I	probably benign	Het
Opcml	G	A	9: 28,812,886 (GRCm39)	E193K	probably damaging	Het
Phf14	T	A	6: 11,988,756 (GRCm39)	N665K	possibly damaging	Het
Plekhh3	G	A	11: 101,056,009 (GRCm39)	A47V	probably damaging	Het
Plekhh3	T	A	11: 101,058,764 (GRCm39)	E156V	probably null	Het
Prpf19	T	C	19: 10,876,323 (GRCm39)		probably benign	Het
Rev3l	T	A	10: 39,697,456 (GRCm39)	V651E	possibly damaging	Het
Sgpl1	T	C	10: 60,948,044 (GRCm39)	N171S	probably damaging	Het
Shroom3	G	T	5: 93,090,945 (GRCm39)	V1151F	probably damaging	Het
Slx4	T	C	16: 3,798,860 (GRCm39)		probably null	Het
Smchd1	T	A	17: 71,665,234 (GRCm39)		probably benign	Het
Snca	A	G	6: 60,792,719 (GRCm39)	V63A	probably benign	Het
Taok3	A	T	5: 117,390,695 (GRCm39)	Q92L	probably damaging	Het
Uhrf1	T	C	17: 56,616,401 (GRCm39)	V73A	probably damaging	Het
Usp38	G	A	8: 81,708,606 (GRCm39)	Q991*	probably null	Het
Vmn2r96	T	A	17: 18,817,866 (GRCm39)	V673E	probably damaging	Het
Zan	A	G	5: 137,436,710 (GRCm39)	Y2048H	unknown	Het
Zfp759	T	A	13: 67,287,354 (GRCm39)	Y302N	probably damaging	Het

Other mutations in Irf9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Irf9	APN	14	55,845,058 (GRCm39)	missense	probably damaging	0.98
IGL02283:Irf9	APN	14	55,845,196 (GRCm39)	missense	probably damaging	1.00
IGL02317:Irf9	APN	14	55,845,196 (GRCm39)	missense	probably damaging	1.00
IGL02407:Irf9	APN	14	55,842,678 (GRCm39)	missense	possibly damaging	0.92
Adjunctive	UTSW	14	55,843,255 (GRCm39)	nonsense	probably null
Long_lost	UTSW	14	55,843,367 (GRCm39)	splice site	probably null
supportive	UTSW	14	55,846,247 (GRCm39)	missense	probably damaging	1.00
R0233:Irf9	UTSW	14	55,843,551 (GRCm39)	missense	probably benign	0.00
R0233:Irf9	UTSW	14	55,843,551 (GRCm39)	missense	probably benign	0.00
R1959:Irf9	UTSW	14	55,845,174 (GRCm39)	missense	possibly damaging	0.93
R2324:Irf9	UTSW	14	55,843,367 (GRCm39)	splice site	probably null
R4669:Irf9	UTSW	14	55,843,223 (GRCm39)	missense	probably benign
R5393:Irf9	UTSW	14	55,843,914 (GRCm39)	unclassified	probably benign
R6072:Irf9	UTSW	14	55,843,284 (GRCm39)	missense	probably damaging	1.00
R6277:Irf9	UTSW	14	55,845,109 (GRCm39)	missense	probably benign	0.04
R6337:Irf9	UTSW	14	55,843,799 (GRCm39)	missense	possibly damaging	0.62
R6545:Irf9	UTSW	14	55,842,684 (GRCm39)	missense	probably damaging	1.00
R6993:Irf9	UTSW	14	55,846,414 (GRCm39)	missense	probably benign	0.06
R7956:Irf9	UTSW	14	55,846,481 (GRCm39)	missense	probably benign	0.00
R8145:Irf9	UTSW	14	55,843,255 (GRCm39)	nonsense	probably null
R8326:Irf9	UTSW	14	55,843,210 (GRCm39)	missense	probably benign	0.02
R9002:Irf9	UTSW	14	55,845,140 (GRCm39)	missense	possibly damaging	0.79
R9293:Irf9	UTSW	14	55,846,247 (GRCm39)	missense	probably damaging	1.00
R9672:Irf9	UTSW	14	55,842,129 (GRCm39)	missense	probably benign	0.05
R9747:Irf9	UTSW	14	55,844,045 (GRCm39)	missense	probably benign	0.00
RF014:Irf9	UTSW	14	55,843,334 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTCAAATAACTGTCCCTGAGGG -3'
(R):5'- AGTTCCTTGGAGCTGAGAGAG -3'

Sequencing Primer
(F):5'- CCTGAGGGGAAGGGGCC -3'
(R):5'- GGAGATAAAAGTCTCTTCCAGGTTG -3'

Posted On

2016-03-17