Incidental Mutation 'R3442:Msmb'
ID 267425
Institutional Source Beutler Lab
Gene Symbol Msmb
Ensembl Gene ENSMUSG00000021907
Gene Name beta-microseminoprotein
Synonyms beta-MSP, PSP94, beta-inhibin, PIP, prostatic inhibin protein
MMRRC Submission 040660-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R3442 (G1)
Quality Score 225
Status Validated
Chromosome 14
Chromosomal Location 31863980-31880284 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 31872173 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Aspartic acid at position 55 (N55D)
Ref Sequence ENSEMBL: ENSMUSP00000022464 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022464] [ENSMUST00000130397]
AlphaFold O08540
Predicted Effect probably benign
Transcript: ENSMUST00000022464
AA Change: N55D

PolyPhen 2 Score 0.088 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000022464
Gene: ENSMUSG00000021907
AA Change: N55D

DomainStartEndE-ValueType
signal peptide 1 20 N/A INTRINSIC
Pfam:PSP94 21 113 6.4e-46 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130397
SMART Domains Protein: ENSMUSP00000121451
Gene: ENSMUSG00000021907

DomainStartEndE-ValueType
signal peptide 1 27 N/A INTRINSIC
Pfam:PSP94 28 120 9.9e-51 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000227860
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.3%
Validation Efficiency 100% (34/34)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer. Two alternatively spliced transcript variants encoding different isoforms are described for this gene. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-in allele exhibit early prostate cancer development progressing from intraepithelial neoplasia with microinvasion to well-differentiated prostate gland adenocarcinoma, and show enlargement of the prostate gland and the lymph nodes, and increased metastatic potential. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 28 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2900092C05Rik T A 7: 12,246,583 (GRCm39) Y26* probably null Het
Adam30 T C 3: 98,069,886 (GRCm39) I573T probably benign Het
Atp4a G A 7: 30,419,650 (GRCm39) R671Q probably benign Het
Cav3 G A 6: 112,449,402 (GRCm39) C140Y possibly damaging Het
Cdh15 G A 8: 123,588,763 (GRCm39) R279Q probably damaging Het
Cfap91 T C 16: 38,154,168 (GRCm39) M126V probably benign Het
Dbt T A 3: 116,341,840 (GRCm39) D480E probably benign Het
Dmbt1 G A 7: 130,707,979 (GRCm39) C1407Y probably damaging Het
Frem3 C T 8: 81,339,669 (GRCm39) P654L probably damaging Het
Glb1l2 C T 9: 26,692,038 (GRCm39) A74T probably damaging Het
Gpx1 C G 9: 108,216,549 (GRCm39) T13S probably benign Het
Grik3 C A 4: 125,587,763 (GRCm39) L628M probably damaging Het
Grik3 T A 4: 125,587,764 (GRCm39) L628Q probably damaging Het
Gsap A G 5: 21,483,125 (GRCm39) Y610C probably damaging Het
Gtf3c6 T A 10: 40,127,169 (GRCm39) E123V probably null Het
Htr3b T C 9: 48,856,815 (GRCm39) D221G probably benign Het
Mx1 T A 16: 97,257,431 (GRCm39) I109F probably damaging Het
Mynn T C 3: 30,667,712 (GRCm39) F471L probably damaging Het
Or9i2 T C 19: 13,816,370 (GRCm39) T56A possibly damaging Het
Otof T C 5: 30,529,033 (GRCm39) R1792G probably damaging Het
Sil1 A T 18: 35,458,449 (GRCm39) L182H probably damaging Het
Sla C T 15: 66,655,509 (GRCm39) G210D probably benign Het
Slc26a7 C T 4: 14,565,511 (GRCm39) V191M probably benign Het
Trrap A G 5: 144,729,062 (GRCm39) M659V probably benign Het
Ubxn6 G T 17: 56,376,049 (GRCm39) Q371K probably benign Het
Zfat A T 15: 67,956,402 (GRCm39) D1143E probably benign Het
Zfat C T 15: 67,973,430 (GRCm39) A1122T probably damaging Het
Zfp950 A T 19: 61,107,170 (GRCm39) C149* probably null Het
Other mutations in Msmb
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1942:Msmb UTSW 14 31,870,034 (GRCm39) missense probably benign 0.00
R8865:Msmb UTSW 14 31,872,217 (GRCm39) nonsense probably null
R9224:Msmb UTSW 14 31,880,060 (GRCm39) nonsense probably null
R9274:Msmb UTSW 14 31,870,130 (GRCm39) missense probably benign 0.01
RF024:Msmb UTSW 14 31,880,047 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCAGGACTCTAGCCCTTTTG -3'
(R):5'- ATGCTTTTCATACAGTGCACAC -3'

Sequencing Primer
(F):5'- GCAGGACTCTAGCCCTTTTGAATAC -3'
(R):5'- GCTTTTCATACAGTGCACACATTAC -3'
Posted On 2015-02-18