Mutagenetix > Incidental Mutation

Incidental Mutation 'R8706:Dynap'

669286

Institutional Source

Beutler Lab

Gene Symbol

Dynap

Ensembl Gene

ENSMUSG00000024512

Gene Name

dynactin associated protein

Synonyms

2310002L13Rik

MMRRC Submission

068560-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R8706 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

70373500-70377653 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 70374062 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 155 (E155K)

Ref Sequence

ENSEMBL: ENSMUSP00000025390 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025390]

AlphaFold

Q9D7M5

Predicted Effect

unknown
Transcript: ENSMUST00000025390
AA Change: E155K

SMART Domains

Protein: ENSMUSP00000025390
Gene: ENSMUSG00000024512
AA Change: E155K

Domain	Start	End	E-Value	Type
Pfam:CLLAC	51	80	2.5e-16	PFAM
low complexity region	104	148	N/A	INTRINSIC

Meta Mutation Damage Score

0.0852

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adpgk	C	T	9: 59,222,456 (GRCm39)	T461I	probably benign	Het
Adra2b	A	G	2: 127,206,487 (GRCm39)	T335A	probably benign	Het
Ap3b1	T	C	13: 94,545,353 (GRCm39)		probably null	Het
C1rl	T	C	6: 124,470,191 (GRCm39)		probably null	Het
Ccdc142	A	G	6: 83,080,678 (GRCm39)	E470G	probably damaging	Het
Ccm2	T	A	11: 6,539,447 (GRCm39)	I163N	possibly damaging	Het
Cdk5rap2	A	T	4: 70,157,562 (GRCm39)	F1596L	probably benign	Het
Chrna4	A	T	2: 180,679,307 (GRCm39)	F47I	probably damaging	Het
Cx3cl1	A	G	8: 95,506,876 (GRCm39)	T294A	probably benign	Het
Dcun1d4	C	T	5: 73,714,658 (GRCm39)	T275M	probably damaging	Het
Dpp4	T	C	2: 62,208,647 (GRCm39)	I166V	probably benign	Het
Fam171b	A	G	2: 83,690,864 (GRCm39)	T276A	probably benign	Het
Fmo4	G	A	1: 162,621,592 (GRCm39)	Q540*	probably null	Het
Gtf2i	C	A	5: 134,278,733 (GRCm39)	V633F	probably damaging	Het
Kmt5c	G	T	7: 4,749,153 (GRCm39)	R262L	probably damaging	Het
Lgr5	A	G	10: 115,288,610 (GRCm39)	L678P	probably benign	Het
Mrps27	T	C	13: 99,541,508 (GRCm39)	V163A	probably damaging	Het
Myh7b	G	A	2: 155,453,669 (GRCm39)		probably null	Het
Myo15a	A	G	11: 60,370,443 (GRCm39)	T1068A	probably benign	Het
Ncor2	T	C	5: 125,145,010 (GRCm39)	E369G	unknown	Het
Neb	T	A	2: 52,181,326 (GRCm39)	M1094L	probably benign	Het
Osbpl10	T	C	9: 115,036,688 (GRCm39)	L228P	probably damaging	Het
Phip	T	A	9: 82,787,765 (GRCm39)	R799S	possibly damaging	Het
Prkn	T	C	17: 11,456,472 (GRCm39)	S99P	probably benign	Het
Rassf5	G	T	1: 131,172,782 (GRCm39)	T29N	probably benign	Het
Spata31g1	A	G	4: 42,971,776 (GRCm39)	K370E	probably benign	Het
Tmprss11g	T	A	5: 86,644,404 (GRCm39)	S147C	probably damaging	Het
Ttn	G	A	2: 76,569,134 (GRCm39)	T27253I	probably damaging	Het
Vmn1r59	A	T	7: 5,457,715 (GRCm39)	V15E	possibly damaging	Het
Wnt6	A	C	1: 74,821,947 (GRCm39)	D176A	possibly damaging	Het

Other mutations in Dynap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0041:Dynap	UTSW	18	70,375,105 (GRCm39)	missense	possibly damaging	0.73
R0528:Dynap	UTSW	18	70,375,165 (GRCm39)	splice site	probably benign
R0720:Dynap	UTSW	18	70,374,055 (GRCm39)	missense	unknown
R2268:Dynap	UTSW	18	70,374,218 (GRCm39)	missense	probably benign	0.02
R4739:Dynap	UTSW	18	70,374,296 (GRCm39)	missense	possibly damaging	0.93
R5802:Dynap	UTSW	18	70,374,073 (GRCm39)	missense	unknown
R6841:Dynap	UTSW	18	70,374,253 (GRCm39)	missense	probably damaging	0.98
R7271:Dynap	UTSW	18	70,374,320 (GRCm39)	missense	possibly damaging	0.71
R7354:Dynap	UTSW	18	70,374,371 (GRCm39)	missense	possibly damaging	0.71
R8018:Dynap	UTSW	18	70,375,093 (GRCm39)	missense	possibly damaging	0.53
R8499:Dynap	UTSW	18	70,374,044 (GRCm39)	missense	unknown
R8975:Dynap	UTSW	18	70,375,093 (GRCm39)	missense	possibly damaging	0.53
Z1177:Dynap	UTSW	18	70,374,101 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGCAAGACTATCACCACATGG -3'
(R):5'- CACATCCATTACAGCTTTGGC -3'

Sequencing Primer
(F):5'- CCACATGGAAAGCAGCATAGATTAG -3'
(R):5'- TATTCACACAGATGGAAGGTCCTGC -3'

Posted On

2021-04-30