Mutagenetix > Incidental Mutation

Incidental Mutation 'R9241:Tmem200c'

700770

Institutional Source

Beutler Lab

Gene Symbol

Tmem200c

Ensembl Gene

ENSMUSG00000095407

Gene Name

transmembrane protein 200C

Synonyms

Gm6338

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.089) question?

Stock #

R9241 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

69144084-69150133 bp(+) (GRCm39)

Type of Mutation

start gained

DNA Base Change (assembly)

G to T at 69144161 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000137246 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000178545]

AlphaFold

J3QK46

Predicted Effect

probably benign
Transcript: ENSMUST00000178545

SMART Domains

Protein: ENSMUSP00000137246
Gene: ENSMUSG00000095407

Domain	Start	End	E-Value	Type
Pfam:DUF2371	14	209	4.1e-65	PFAM
SCOP:d1gkub1	227	258	2e-3	SMART
low complexity region	272	291	N/A	INTRINSIC
low complexity region	347	377	N/A	INTRINSIC
low complexity region	382	398	N/A	INTRINSIC
low complexity region	473	486	N/A	INTRINSIC
low complexity region	496	509	N/A	INTRINSIC
low complexity region	538	550	N/A	INTRINSIC
low complexity region	584	599	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (51/52)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030J22Rik	A	G	8: 117,697,937 (GRCm39)	L390P	probably damaging	Het
AL732309.1	T	A	2: 25,135,919 (GRCm39)	E98D	possibly damaging	Het
Aldh2	T	C	5: 121,710,220 (GRCm39)	I372V	probably benign	Het
Amph	G	T	13: 19,278,972 (GRCm39)	R149L	probably damaging	Het
Ano6	T	C	15: 95,688,887 (GRCm39)	V8A	probably benign	Het
Aox4	T	A	1: 58,291,345 (GRCm39)	V821E	probably damaging	Het
Baz2b	T	A	2: 59,743,993 (GRCm39)	Q1504L	probably benign	Het
Cdcp1	C	A	9: 123,014,301 (GRCm39)	G158W	probably damaging	Het
Cryge	T	G	1: 65,088,018 (GRCm39)	D97A	possibly damaging	Het
Dusp9	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	X: 72,684,217 (GRCm39)		probably benign	Het
Dyrk1b	A	G	7: 27,886,058 (GRCm39)	T594A	probably benign	Het
Emilin3	T	C	2: 160,750,177 (GRCm39)	D477G	possibly damaging	Het
Fat2	T	C	11: 55,147,566 (GRCm39)	H3892R	probably benign	Het
Fktn	G	A	4: 53,734,854 (GRCm39)	G125D	probably benign	Het
Gm9922	A	T	14: 101,967,220 (GRCm39)	S11T	unknown	Het
Hcfc2	T	C	10: 82,568,485 (GRCm39)	V43A	probably benign	Het
Hcn1	T	C	13: 117,793,249 (GRCm39)	F167S	probably benign	Het
Jag1	T	C	2: 136,926,507 (GRCm39)	I1012M	probably damaging	Het
Kash5	A	G	7: 44,833,313 (GRCm39)	I603T	probably benign	Het
Lepr	G	T	4: 101,671,788 (GRCm39)	M937I	probably benign	Het
Macf1	T	C	4: 123,271,952 (GRCm39)	D6532G	probably damaging	Het
Mapk13	A	T	17: 28,990,187 (GRCm39)	D102V	probably damaging	Het
Mapt	A	T	11: 104,189,797 (GRCm39)	T272S	probably benign	Het
Mfap3	A	G	11: 57,420,672 (GRCm39)	T218A	probably damaging	Het
Mki67	A	T	7: 135,297,653 (GRCm39)	H2460Q	possibly damaging	Het
Nalcn	G	T	14: 123,809,429 (GRCm39)	P241Q	probably benign	Het
Nup50	A	G	15: 84,822,611 (GRCm39)	T378A	possibly damaging	Het
Oprl1	C	T	2: 181,360,405 (GRCm39)	R154C	probably damaging	Het
Or2n1e	A	G	17: 38,585,781 (GRCm39)	M40V	probably benign	Het
Pcdha8	C	T	18: 37,127,008 (GRCm39)	R497W	probably damaging	Het
Pik3c2a	A	G	7: 116,017,115 (GRCm39)	V214A	probably benign	Het
Plekha5	T	C	6: 140,525,204 (GRCm39)		probably null	Het
Pole3	A	G	4: 62,442,845 (GRCm39)		probably benign	Het
Prkn	A	T	17: 11,456,382 (GRCm39)	I69L	probably benign	Het
Psd3	G	T	8: 68,415,967 (GRCm39)	N357K	probably benign	Het
Psme4	A	T	11: 30,815,576 (GRCm39)	D1696V	probably damaging	Het
Reln	C	T	5: 22,174,067 (GRCm39)	R2012Q	probably damaging	Het
Saxo2	T	C	7: 82,284,250 (GRCm39)	T203A	probably benign	Het
Scgb1a1	T	C	19: 9,065,293 (GRCm39)		probably benign	Het
Selplg	T	A	5: 113,957,647 (GRCm39)	N220Y	possibly damaging	Het
Septin9	C	A	11: 117,109,724 (GRCm39)	R15S	probably benign	Het
Slc4a11	C	T	2: 130,533,664 (GRCm39)	A100T	probably damaging	Het
Smarca4	T	G	9: 21,550,604 (GRCm39)	S446A	possibly damaging	Het
Smc1b	T	C	15: 84,976,209 (GRCm39)	N833D	probably benign	Het
Son	T	A	16: 91,454,122 (GRCm39)	D956E	probably damaging	Het
Sorl1	A	T	9: 41,885,420 (GRCm39)	Y2060*	probably null	Het
Tesmin	T	C	19: 3,439,010 (GRCm39)	F21L	probably benign	Het
Tnk2	A	C	16: 32,488,916 (GRCm39)	D252A	probably damaging	Het
Tpcn1	C	T	5: 120,691,558 (GRCm39)	V299I	probably benign	Het
Trpv1	A	G	11: 73,151,182 (GRCm39)	N790S	probably benign	Het
Zfhx4	C	T	3: 5,308,697 (GRCm39)	T641I	probably damaging	Het
Zfp563	A	T	17: 33,321,520 (GRCm39)	Q45L	probably benign	Het
Zswim9	A	T	7: 13,003,360 (GRCm39)	D163E	probably damaging	Het

Other mutations in Tmem200c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0360:Tmem200c	UTSW	17	69,147,543 (GRCm39)	missense	probably damaging	1.00
R0364:Tmem200c	UTSW	17	69,147,543 (GRCm39)	missense	probably damaging	1.00
R0599:Tmem200c	UTSW	17	69,147,506 (GRCm39)	missense	probably damaging	1.00
R0711:Tmem200c	UTSW	17	69,149,249 (GRCm39)	missense	probably damaging	1.00
R1311:Tmem200c	UTSW	17	69,147,758 (GRCm39)	missense	probably damaging	0.98
R1852:Tmem200c	UTSW	17	69,147,612 (GRCm39)	missense	probably damaging	1.00
R1951:Tmem200c	UTSW	17	69,147,983 (GRCm39)	missense	probably damaging	1.00
R1954:Tmem200c	UTSW	17	69,147,956 (GRCm39)	missense	probably damaging	1.00
R1955:Tmem200c	UTSW	17	69,147,956 (GRCm39)	missense	probably damaging	1.00
R2144:Tmem200c	UTSW	17	69,149,244 (GRCm39)	missense	possibly damaging	0.49
R2189:Tmem200c	UTSW	17	69,147,681 (GRCm39)	missense	probably damaging	0.98
R2397:Tmem200c	UTSW	17	69,147,942 (GRCm39)	missense	probably damaging	1.00
R4546:Tmem200c	UTSW	17	69,149,166 (GRCm39)	missense	probably benign
R4715:Tmem200c	UTSW	17	69,147,465 (GRCm39)	missense	probably damaging	1.00
R4752:Tmem200c	UTSW	17	69,149,235 (GRCm39)	missense	probably benign	0.05
R5214:Tmem200c	UTSW	17	69,148,122 (GRCm39)	missense	probably damaging	1.00
R5751:Tmem200c	UTSW	17	69,147,547 (GRCm39)	missense	probably damaging	1.00
R5827:Tmem200c	UTSW	17	69,149,004 (GRCm39)	missense	probably benign	0.00
R5989:Tmem200c	UTSW	17	69,144,431 (GRCm39)	start gained	probably benign
R6024:Tmem200c	UTSW	17	69,148,722 (GRCm39)	missense	possibly damaging	0.49
R6634:Tmem200c	UTSW	17	69,149,101 (GRCm39)	missense	probably benign	0.26
R7527:Tmem200c	UTSW	17	69,148,671 (GRCm39)	missense	probably benign	0.04
R8046:Tmem200c	UTSW	17	69,147,513 (GRCm39)	missense	probably benign	0.29
R8927:Tmem200c	UTSW	17	69,148,733 (GRCm39)	missense	probably benign	0.00
R8928:Tmem200c	UTSW	17	69,148,733 (GRCm39)	missense	probably benign	0.00
R9185:Tmem200c	UTSW	17	69,147,633 (GRCm39)	missense	probably damaging	1.00
R9374:Tmem200c	UTSW	17	69,148,682 (GRCm39)	missense	probably damaging	1.00
R9651:Tmem200c	UTSW	17	69,149,181 (GRCm39)	missense	probably benign	0.02
R9652:Tmem200c	UTSW	17	69,149,181 (GRCm39)	missense	probably benign	0.02
R9653:Tmem200c	UTSW	17	69,149,181 (GRCm39)	missense	probably benign	0.02
R9775:Tmem200c	UTSW	17	69,149,118 (GRCm39)	missense	probably damaging	1.00
Z1176:Tmem200c	UTSW	17	69,148,790 (GRCm39)	missense	probably damaging	0.99
Z1177:Tmem200c	UTSW	17	69,148,339 (GRCm39)	missense	probably benign	0.26
Z1177:Tmem200c	UTSW	17	69,148,332 (GRCm39)	missense	probably benign	0.13
Z1177:Tmem200c	UTSW	17	69,148,431 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACCTTGCCTTACACGATCG -3'
(R):5'- AAGAAGTGTCAGGTCCTCCG -3'

Sequencing Primer
(F):5'- ACCTGGTTCAAGCACGC -3'
(R):5'- GTGTCAGGTCCTCCGCATCC -3'

Posted On

2022-02-07