Incidental Mutation 'R1642:Oas3'
ID 173596
Institutional Source Beutler Lab
Gene Symbol Oas3
Ensembl Gene ENSMUSG00000032661
Gene Name 2'-5' oligoadenylate synthetase 3
Synonyms Oasl10, 2'-5' oligoadenylate synthetase-like 10
MMRRC Submission 039678-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.092) question?
Stock # R1642 (G1)
Quality Score 166
Status Not validated
Chromosome 5
Chromosomal Location 120891163-120915726 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 120915639 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Histidine to Glutamine at position 17 (H17Q)
Ref Sequence ENSEMBL: ENSMUSP00000035588 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000044833]
AlphaFold Q8VI93
Predicted Effect possibly damaging
Transcript: ENSMUST00000044833
AA Change: H17Q

PolyPhen 2 Score 0.897 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000035588
Gene: ENSMUSG00000032661
AA Change: H17Q

DomainStartEndE-ValueType
Pfam:OAS1_C 159 341 6.3e-83 PFAM
Pfam:OAS1_C 610 795 3.1e-78 PFAM
Pfam:NTP_transf_2 831 920 4.5e-11 PFAM
Pfam:OAS1_C 954 1136 9e-85 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000166703
Predicted Effect noncoding transcript
Transcript: ENSMUST00000201006
Coding Region Coverage
  • 1x: 99.0%
  • 3x: 98.1%
  • 10x: 95.4%
  • 20x: 89.7%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2310057J18Rik A G 10: 28,862,233 (GRCm39) V19A probably benign Het
6820408C15Rik T C 2: 152,282,774 (GRCm39) Y210H probably damaging Het
Aars1 T A 8: 111,769,882 (GRCm39) I327N possibly damaging Het
Abca6 T A 11: 110,109,107 (GRCm39) N688Y possibly damaging Het
Ablim3 T C 18: 61,947,382 (GRCm39) K457R probably benign Het
Acsm2 A T 7: 119,162,860 (GRCm39) N45Y probably damaging Het
Agxt2 T C 15: 10,373,917 (GRCm39) S108P probably damaging Het
Akr1cl A T 1: 65,060,588 (GRCm39) M174K probably benign Het
Armh4 T C 14: 50,005,867 (GRCm39) probably null Het
Bfsp1 G A 2: 143,683,683 (GRCm39) R214W probably damaging Het
Cby3 A G 11: 50,250,343 (GRCm39) D183G probably damaging Het
Clip2 T C 5: 134,532,107 (GRCm39) D566G possibly damaging Het
Colgalt1 A G 8: 72,073,401 (GRCm39) I341V probably benign Het
Cyp2c38 T A 19: 39,390,153 (GRCm39) D349V probably damaging Het
Cyp3a16 T A 5: 145,406,399 (GRCm39) I18F unknown Het
Degs2 C T 12: 108,658,451 (GRCm39) C176Y probably benign Het
Dhx16 A T 17: 36,201,957 (GRCm39) T995S probably damaging Het
Dicer1 A T 12: 104,679,415 (GRCm39) C521S probably damaging Het
Dop1b T C 16: 93,559,203 (GRCm39) S532P probably benign Het
Dpysl4 T G 7: 138,670,254 (GRCm39) M124R probably damaging Het
Eml6 A G 11: 29,727,001 (GRCm39) probably null Het
Erbb4 A T 1: 68,370,393 (GRCm39) V395D probably damaging Het
Esf1 T C 2: 140,000,406 (GRCm39) D460G possibly damaging Het
F830045P16Rik G A 2: 129,305,634 (GRCm39) H247Y probably benign Het
Fsbp T A 4: 11,583,965 (GRCm39) S221R probably benign Het
Fstl5 T A 3: 76,317,929 (GRCm39) N198K possibly damaging Het
Gemin5 G T 11: 58,029,906 (GRCm39) H855Q probably damaging Het
Gjd3 T C 11: 98,873,535 (GRCm39) E103G probably benign Het
I0C0044D17Rik A G 4: 98,708,471 (GRCm39) probably benign Het
Iqca1l C A 5: 24,757,686 (GRCm39) R177L probably damaging Het
Itgb4 A T 11: 115,898,183 (GRCm39) R1646W probably damaging Het
Klri2 A T 6: 129,715,837 (GRCm39) C121S probably benign Het
Lamc3 A G 2: 31,806,008 (GRCm39) Y703C probably damaging Het
Lrrc10 A G 10: 116,881,788 (GRCm39) N154S probably damaging Het
Lrriq1 A G 10: 103,050,317 (GRCm39) F812L probably benign Het
Naip2 C T 13: 100,298,489 (GRCm39) A516T possibly damaging Het
Nav1 T C 1: 135,380,010 (GRCm39) Y1564C probably damaging Het
Ndufc1 T C 3: 51,315,664 (GRCm39) T25A probably benign Het
Neurl4 A G 11: 69,794,485 (GRCm39) M23V probably benign Het
Nnt A G 13: 119,541,086 (GRCm39) probably null Het
Nolc1 G C 19: 46,067,461 (GRCm39) probably null Het
Nrg1 A T 8: 32,314,536 (GRCm39) M289K probably benign Het
Or10h1 A G 17: 33,418,430 (GRCm39) Y132C probably damaging Het
Or2z2 A G 11: 58,346,664 (GRCm39) I37T probably benign Het
Or5m5 A G 2: 85,814,201 (GRCm39) K6E probably benign Het
Or8g36 C T 9: 39,422,650 (GRCm39) R122H possibly damaging Het
Parp9 T C 16: 35,788,067 (GRCm39) Y612H probably benign Het
Pcdh1 A T 18: 38,332,283 (GRCm39) M240K possibly damaging Het
Pcdhb9 A G 18: 37,533,987 (GRCm39) probably benign Het
Plpp2 A G 10: 79,366,518 (GRCm39) V42A probably damaging Het
Ppic C T 18: 53,540,134 (GRCm39) V172M probably damaging Het
Ppp1r9b A G 11: 94,892,150 (GRCm39) silent Het
Prop1 A G 11: 50,844,152 (GRCm39) V27A possibly damaging Het
Psmc5 A G 11: 106,153,242 (GRCm39) T295A probably benign Het
Rpa1 A G 11: 75,203,517 (GRCm39) probably null Het
Rrp12 A G 19: 41,860,176 (GRCm39) F1016L probably damaging Het
Scn2a A T 2: 65,514,041 (GRCm39) I242F probably damaging Het
Slco1a6 T A 6: 142,032,160 (GRCm39) H655L probably benign Het
Sp140 G A 1: 85,538,545 (GRCm39) probably null Het
Syne1 A G 10: 5,298,694 (GRCm39) I1071T possibly damaging Het
Tbc1d9b A G 11: 50,040,659 (GRCm39) D392G probably damaging Het
Tcaim G A 9: 122,647,838 (GRCm39) probably null Het
Tgm3 T A 2: 129,889,702 (GRCm39) V632E probably damaging Het
Triobp C A 15: 78,886,348 (GRCm39) R1830S probably damaging Het
Ttn A T 2: 76,641,587 (GRCm39) L5176Q possibly damaging Het
Vps8 A G 16: 21,400,329 (GRCm39) T1266A probably benign Het
Znfx1 T C 2: 166,880,930 (GRCm39) I285V possibly damaging Het
Other mutations in Oas3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00547:Oas3 APN 5 120,915,507 (GRCm39) splice site probably benign
IGL01095:Oas3 APN 5 120,910,954 (GRCm39) missense probably damaging 1.00
IGL01835:Oas3 APN 5 120,904,193 (GRCm39) nonsense probably null
IGL02006:Oas3 APN 5 120,907,300 (GRCm39) missense probably benign 0.00
IGL02811:Oas3 APN 5 120,902,387 (GRCm39) missense unknown
IGL03194:Oas3 APN 5 120,897,018 (GRCm39) missense probably damaging 1.00
R0066:Oas3 UTSW 5 120,896,940 (GRCm39) missense probably damaging 1.00
R0195:Oas3 UTSW 5 120,894,210 (GRCm39) missense probably damaging 1.00
R0196:Oas3 UTSW 5 120,894,210 (GRCm39) missense probably damaging 1.00
R0197:Oas3 UTSW 5 120,894,210 (GRCm39) missense probably damaging 1.00
R0445:Oas3 UTSW 5 120,894,210 (GRCm39) missense probably damaging 1.00
R0523:Oas3 UTSW 5 120,904,209 (GRCm39) missense unknown
R0592:Oas3 UTSW 5 120,909,214 (GRCm39) missense probably damaging 1.00
R0946:Oas3 UTSW 5 120,907,128 (GRCm39) missense unknown
R1354:Oas3 UTSW 5 120,908,065 (GRCm39) missense possibly damaging 0.94
R1681:Oas3 UTSW 5 120,907,973 (GRCm39) missense probably benign 0.22
R1844:Oas3 UTSW 5 120,898,045 (GRCm39) missense probably damaging 0.99
R1981:Oas3 UTSW 5 120,899,900 (GRCm39) splice site probably benign
R2443:Oas3 UTSW 5 120,915,553 (GRCm39) missense probably benign 0.35
R2902:Oas3 UTSW 5 120,896,982 (GRCm39) missense probably damaging 1.00
R3034:Oas3 UTSW 5 120,909,121 (GRCm39) missense probably damaging 1.00
R4565:Oas3 UTSW 5 120,909,104 (GRCm39) missense probably damaging 1.00
R4692:Oas3 UTSW 5 120,907,420 (GRCm39) missense probably benign 0.03
R4723:Oas3 UTSW 5 120,904,321 (GRCm39) missense unknown
R4812:Oas3 UTSW 5 120,899,212 (GRCm39) unclassified probably benign
R5288:Oas3 UTSW 5 120,895,055 (GRCm39) missense probably damaging 1.00
R5343:Oas3 UTSW 5 120,894,303 (GRCm39) missense possibly damaging 0.70
R5494:Oas3 UTSW 5 120,899,709 (GRCm39) missense unknown
R5688:Oas3 UTSW 5 120,896,867 (GRCm39) missense probably benign 0.31
R5894:Oas3 UTSW 5 120,895,019 (GRCm39) missense probably damaging 1.00
R5921:Oas3 UTSW 5 120,908,046 (GRCm39) missense probably damaging 1.00
R6037:Oas3 UTSW 5 120,907,384 (GRCm39) missense probably benign 0.41
R6037:Oas3 UTSW 5 120,907,384 (GRCm39) missense probably benign 0.41
R6066:Oas3 UTSW 5 120,910,989 (GRCm39) missense probably damaging 0.97
R6104:Oas3 UTSW 5 120,899,758 (GRCm39) missense unknown
R6134:Oas3 UTSW 5 120,907,113 (GRCm39) missense unknown
R6255:Oas3 UTSW 5 120,909,295 (GRCm39) missense probably benign 0.04
R6257:Oas3 UTSW 5 120,899,200 (GRCm39) unclassified probably benign
R6776:Oas3 UTSW 5 120,896,939 (GRCm39) missense probably damaging 1.00
R8022:Oas3 UTSW 5 120,895,031 (GRCm39) missense possibly damaging 0.91
R8137:Oas3 UTSW 5 120,915,565 (GRCm39) missense probably benign 0.07
R8967:Oas3 UTSW 5 120,896,907 (GRCm39) missense probably damaging 0.99
R9124:Oas3 UTSW 5 120,912,170 (GRCm39) missense probably damaging 1.00
R9287:Oas3 UTSW 5 120,892,754 (GRCm39) missense probably damaging 1.00
R9661:Oas3 UTSW 5 120,904,230 (GRCm39) missense unknown
R9745:Oas3 UTSW 5 120,899,284 (GRCm39) missense unknown
RF006:Oas3 UTSW 5 120,912,165 (GRCm39) missense probably damaging 1.00
X0024:Oas3 UTSW 5 120,899,793 (GRCm39) missense unknown
Predicted Primers PCR Primer
(F):5'- TCCCTCAAGAAGACCCTGAAGGTG -3'
(R):5'- TCCCCAAATAGGAGGTCAGGAAGC -3'

Sequencing Primer
(F):5'- AAGACCCTGAAGGTGTTTGCC -3'
(R):5'- CCCAGAGGGTCAGAAGTCAC -3'
Posted On 2014-04-24