Mutagenetix > Incidental Mutation

Incidental Mutation 'R2154:Smg1'

234620

Institutional Source

Beutler Lab

Gene Symbol

Smg1

Ensembl Gene

ENSMUSG00000030655

Gene Name

SMG1 nonsense mediated mRNA decay associated PI3K related kinase

Synonyms

5430435M13Rik, SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans), 2610207I05Rik, C130002K18Rik

MMRRC Submission

040157-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R2154 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

117730531-117842893 bp(-) (GRCm39)

Type of Mutation

utr 3 prime

DNA Base Change (assembly)

A to T at 117757299 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032891]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000032891
AA Change: M2286K

SMART Domains

Protein: ENSMUSP00000032891
Gene: ENSMUSG00000030655
AA Change: M2286K

Domain	Start	End	E-Value	Type
low complexity region	42	55	N/A	INTRINSIC
SCOP:d1gw5a_	147	621	7e-7	SMART
Pfam:SMG1	629	1240	9.8e-249	PFAM
low complexity region	1540	1551	N/A	INTRINSIC
SCOP:d1gw5a_	1680	1942	8e-3	SMART
low complexity region	2125	2141	N/A	INTRINSIC
PI3Kc	2149	2493	7.93e-50	SMART
low complexity region	2759	2770	N/A	INTRINSIC
low complexity region	3425	3442	N/A	INTRINSIC
FATC	3626	3658	8.66e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000083940

Predicted Effect

probably benign
Transcript: ENSMUST00000179331

SMART Domains

Protein: ENSMUSP00000137592
Gene: ENSMUSG00000030655

Domain	Start	End	E-Value	Type
low complexity region	18	31	N/A	INTRINSIC
SCOP:d1gw5a_	71	545	1e-6	SMART
low complexity region	602	612	N/A	INTRINSIC
low complexity region	631	646	N/A	INTRINSIC
low complexity region	698	718	N/A	INTRINSIC
low complexity region	898	915	N/A	INTRINSIC
low complexity region	1135	1147	N/A	INTRINSIC
low complexity region	1464	1475	N/A	INTRINSIC
low complexity region	2049	2065	N/A	INTRINSIC
PI3Kc	2073	2417	7.93e-50	SMART
low complexity region	2683	2694	N/A	INTRINSIC
low complexity region	3349	3366	N/A	INTRINSIC
FATC	3550	3582	8.66e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208025

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for a gene trap allele exhibit early embryonic lethality. Mice heteroygous for a gene trap allele exhibit abnormal tooth development, chronic inflammation, increased body weight, increased incidence of tumor formation and premature death. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030K09Rik	A	G	8: 73,208,959 (GRCm39)	E363G	probably benign	Het
Abca3	A	G	17: 24,596,693 (GRCm39)	Y382C	probably damaging	Het
Abca5	A	G	11: 110,183,000 (GRCm39)	I1019T	probably benign	Het
Acox3	A	T	5: 35,762,568 (GRCm39)	S481C	probably damaging	Het
Ankrd7	T	A	6: 18,870,030 (GRCm39)	M261K	probably benign	Het
Aqr	A	C	2: 113,967,485 (GRCm39)	M510R	probably damaging	Het
Arpc1a	A	T	5: 145,029,369 (GRCm39)	T56S	probably benign	Het
Asap2	A	T	12: 21,162,084 (GRCm39)	T14S	probably damaging	Het
Aspg	A	G	12: 112,087,408 (GRCm39)	E288G	probably benign	Het
Atp2c2	A	G	8: 120,482,841 (GRCm39)	N901S	probably benign	Het
Cabcoco1	A	G	10: 68,267,092 (GRCm39)	L205P	probably damaging	Het
Cant1	A	G	11: 118,302,263 (GRCm39)	L18P	probably damaging	Het
Cdca2	G	A	14: 67,914,425 (GRCm39)	P945S	probably damaging	Het
Cfap74	A	G	4: 155,513,753 (GRCm39)	K522E	possibly damaging	Het
Chek1	C	A	9: 36,635,279 (GRCm39)	V35F	probably damaging	Het
Cpa6	A	T	1: 10,407,547 (GRCm39)	M330K	probably damaging	Het
Creb3l4	G	T	3: 90,145,792 (GRCm39)	N246K	probably damaging	Het
Cyp2c55	T	A	19: 39,022,819 (GRCm39)	V319D	probably damaging	Het
Dapk1	T	A	13: 60,877,317 (GRCm39)	S519T	probably benign	Het
Dhx38	T	C	8: 110,287,306 (GRCm39)	S221G	probably benign	Het
Dis3l	T	A	9: 64,214,545 (GRCm39)	N981I	probably benign	Het
Dock4	T	A	12: 40,870,661 (GRCm39)	V1467E	probably damaging	Het
Dock4	ACCTGCTCTGCC	ACCTGCTCTGCCTGCTCTGCC	12: 40,894,547 (GRCm39)		probably benign	Het
Dok5	G	A	2: 170,642,816 (GRCm39)	G38D	probably damaging	Het
Ercc6l2	T	C	13: 64,013,821 (GRCm39)	S631P	probably damaging	Het
Fat4	A	T	3: 38,941,688 (GRCm39)	S194C	probably damaging	Het
Fcsk	T	A	8: 111,615,704 (GRCm39)	T542S	probably benign	Het
Garem2	T	C	5: 30,313,297 (GRCm39)	S54P	probably damaging	Het
Gdf10	T	C	14: 33,656,346 (GRCm39)	I436T	probably damaging	Het
Gfod1	T	C	13: 43,456,946 (GRCm39)	T10A	possibly damaging	Het
Gucy1a1	A	G	3: 82,018,458 (GRCm39)		probably null	Het
Heatr5b	G	T	17: 79,138,873 (GRCm39)	Q90K	probably benign	Het
Ikzf3	T	A	11: 98,376,475 (GRCm39)	K211*	probably null	Het
Itgam	A	C	7: 127,684,749 (GRCm39)	D373A	probably damaging	Het
Itpripl2	A	G	7: 118,089,107 (GRCm39)	F484S	probably damaging	Het
Kat6b	A	T	14: 21,718,735 (GRCm39)	H1138L	probably benign	Het
Kif16b	T	C	2: 142,532,500 (GRCm39)	K1213R	probably damaging	Het
Klra3	G	C	6: 130,310,107 (GRCm39)	R138G	probably benign	Het
Mmp25	T	A	17: 23,850,048 (GRCm39)	Y504F	probably damaging	Het
Mtf2	A	G	5: 108,228,797 (GRCm39)	K38E	possibly damaging	Het
Myh14	A	C	7: 44,301,853 (GRCm39)		probably null	Het
Nedd4l	T	A	18: 65,343,401 (GRCm39)	H820Q	probably damaging	Het
Nfkb1	T	C	3: 135,307,240 (GRCm39)	T562A	probably benign	Het
Or8b49	G	T	9: 38,505,707 (GRCm39)	L63F	probably damaging	Het
Pds5a	A	T	5: 65,807,841 (GRCm39)	V464E	probably damaging	Het
Peak1	C	T	9: 56,114,496 (GRCm39)	V452M	probably damaging	Het
Phf2	T	C	13: 48,973,549 (GRCm39)	Y372C	unknown	Het
Poc1a	T	A	9: 106,162,773 (GRCm39)		probably null	Het
Prss33	C	T	17: 24,053,817 (GRCm39)	V87M	probably damaging	Het
Psmc2	C	G	5: 22,008,127 (GRCm39)	L344V	possibly damaging	Het
Ptpn12	C	A	5: 21,207,466 (GRCm39)	Q297H	probably damaging	Het
Rabgap1	T	C	2: 37,365,453 (GRCm39)	V242A	probably damaging	Het
Rad1	C	A	15: 10,486,721 (GRCm39)	H39Q	possibly damaging	Het
Rad51ap2	A	G	12: 11,507,986 (GRCm39)	H636R	probably benign	Het
Samd9l	C	T	6: 3,372,945 (GRCm39)	D1439N	possibly damaging	Het
Sbno1	A	T	5: 124,516,574 (GRCm39)	D1266E	probably benign	Het
Sidt2	T	C	9: 45,856,638 (GRCm39)	D477G	probably damaging	Het
Slc22a13	T	C	9: 119,037,753 (GRCm39)	K125R	probably benign	Het
Slc6a1	G	T	6: 114,284,731 (GRCm39)	G263V	probably damaging	Het
Slmap	A	G	14: 26,139,402 (GRCm39)	Y771H	probably damaging	Het
Spinkl	A	T	18: 44,302,194 (GRCm39)	N32K	probably benign	Het
Stxbp1	T	A	2: 32,692,868 (GRCm39)	I383F	probably damaging	Het
Taf3	C	T	2: 9,956,377 (GRCm39)	E597K	possibly damaging	Het
Tln2	T	C	9: 67,209,842 (GRCm39)	T432A	probably damaging	Het
Tmco6	T	C	18: 36,874,740 (GRCm39)	V409A	probably benign	Het
Tstd2	T	C	4: 46,129,235 (GRCm39)	T198A	probably damaging	Het
Vmn1r230	T	A	17: 21,067,063 (GRCm39)	M84K	probably damaging	Het
Vmn1r45	T	A	6: 89,910,965 (GRCm39)	S2C	possibly damaging	Het
Vmn2r25	T	A	6: 123,816,805 (GRCm39)	T259S	probably benign	Het
Vmn2r70	A	G	7: 85,212,923 (GRCm39)	S495P	possibly damaging	Het
Vmn2r97	A	T	17: 19,167,584 (GRCm39)	R613*	probably null	Het
Yme1l1	T	C	2: 23,052,520 (GRCm39)	L58P	probably damaging	Het
Zan	G	A	5: 137,412,511 (GRCm39)		probably benign	Het
Zfhx4	A	G	3: 5,466,801 (GRCm39)	T2320A	possibly damaging	Het
Zfp407	T	C	18: 84,227,774 (GRCm39)	D1945G	possibly damaging	Het

Other mutations in Smg1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Smg1	APN	7	117,797,494 (GRCm39)	utr 3 prime	probably benign
IGL00481:Smg1	APN	7	117,810,017 (GRCm39)	missense	possibly damaging	0.67
IGL00503:Smg1	APN	7	117,784,706 (GRCm39)	utr 3 prime	probably benign
IGL00927:Smg1	APN	7	117,739,855 (GRCm39)	missense	probably damaging	1.00
IGL01333:Smg1	APN	7	117,762,601 (GRCm39)	splice site	probably benign
IGL01344:Smg1	APN	7	117,790,059 (GRCm39)	utr 3 prime	probably benign
IGL01397:Smg1	APN	7	117,762,444 (GRCm39)	utr 3 prime	probably benign
IGL01403:Smg1	APN	7	117,757,355 (GRCm39)	utr 3 prime	probably benign
IGL01573:Smg1	APN	7	117,767,185 (GRCm39)	utr 3 prime	probably benign
IGL01872:Smg1	APN	7	117,748,167 (GRCm39)	utr 3 prime	probably benign
IGL02010:Smg1	APN	7	117,785,369 (GRCm39)	utr 3 prime	probably benign
IGL02158:Smg1	APN	7	117,812,169 (GRCm39)	missense	possibly damaging	0.77
IGL02268:Smg1	APN	7	117,781,764 (GRCm39)	missense	probably benign	0.19
IGL02314:Smg1	APN	7	117,753,932 (GRCm39)	utr 3 prime	probably benign
IGL02552:Smg1	APN	7	117,795,117 (GRCm39)	utr 3 prime	probably benign
IGL02577:Smg1	APN	7	117,802,345 (GRCm39)	missense	probably damaging	0.99
IGL02859:Smg1	APN	7	117,748,156 (GRCm39)	utr 3 prime	probably benign
IGL02890:Smg1	APN	7	117,784,724 (GRCm39)	utr 3 prime	probably benign
IGL02892:Smg1	APN	7	117,767,178 (GRCm39)	utr 3 prime	probably benign
IGL03119:Smg1	APN	7	117,794,336 (GRCm39)	utr 3 prime	probably benign
IGL03123:Smg1	APN	7	117,756,404 (GRCm39)	utr 3 prime	probably benign
IGL03128:Smg1	APN	7	117,802,282 (GRCm39)	missense	probably benign	0.03
IGL03184:Smg1	APN	7	117,779,603 (GRCm39)	missense	possibly damaging	0.86
PIT4508001:Smg1	UTSW	7	117,784,764 (GRCm39)	missense	unknown
R0010:Smg1	UTSW	7	117,771,082 (GRCm39)	utr 3 prime	probably benign
R0010:Smg1	UTSW	7	117,771,082 (GRCm39)	utr 3 prime	probably benign
R0025:Smg1	UTSW	7	117,811,666 (GRCm39)	missense	possibly damaging	0.92
R0025:Smg1	UTSW	7	117,811,666 (GRCm39)	missense	possibly damaging	0.92
R0098:Smg1	UTSW	7	117,744,690 (GRCm39)	missense	probably benign	0.02
R0139:Smg1	UTSW	7	117,751,898 (GRCm39)	critical splice donor site	probably null
R0371:Smg1	UTSW	7	117,767,523 (GRCm39)	utr 3 prime	probably benign
R0415:Smg1	UTSW	7	117,781,691 (GRCm39)	missense	probably benign	0.34
R0416:Smg1	UTSW	7	117,783,684 (GRCm39)	splice site	probably benign
R0423:Smg1	UTSW	7	117,776,103 (GRCm39)	missense	possibly damaging	0.53
R0600:Smg1	UTSW	7	117,759,606 (GRCm39)	utr 3 prime	probably benign
R0626:Smg1	UTSW	7	117,781,606 (GRCm39)	missense	possibly damaging	0.82
R0627:Smg1	UTSW	7	117,767,084 (GRCm39)	utr 3 prime	probably benign
R0727:Smg1	UTSW	7	117,765,645 (GRCm39)	utr 3 prime	probably benign
R0729:Smg1	UTSW	7	117,745,512 (GRCm39)	utr 3 prime	probably benign
R0841:Smg1	UTSW	7	117,742,524 (GRCm39)	missense	possibly damaging	0.96
R1114:Smg1	UTSW	7	117,759,013 (GRCm39)	utr 3 prime	probably benign
R1256:Smg1	UTSW	7	117,802,310 (GRCm39)	missense	probably damaging	1.00
R1298:Smg1	UTSW	7	117,767,434 (GRCm39)	utr 3 prime	probably benign
R1370:Smg1	UTSW	7	117,758,975 (GRCm39)	utr 3 prime	probably benign
R1591:Smg1	UTSW	7	117,756,142 (GRCm39)	utr 3 prime	probably benign
R1736:Smg1	UTSW	7	117,765,190 (GRCm39)	splice site	probably null
R1755:Smg1	UTSW	7	117,802,287 (GRCm39)	nonsense	probably null
R1765:Smg1	UTSW	7	117,738,938 (GRCm39)	missense	probably benign	0.03
R1789:Smg1	UTSW	7	117,745,021 (GRCm39)	missense	possibly damaging	0.73
R1845:Smg1	UTSW	7	117,753,845 (GRCm39)	utr 3 prime	probably benign
R1908:Smg1	UTSW	7	117,753,422 (GRCm39)	utr 3 prime	probably benign
R1909:Smg1	UTSW	7	117,753,422 (GRCm39)	utr 3 prime	probably benign
R1942:Smg1	UTSW	7	117,757,326 (GRCm39)	utr 3 prime	probably benign
R2064:Smg1	UTSW	7	117,756,090 (GRCm39)	utr 3 prime	probably benign
R2072:Smg1	UTSW	7	117,762,389 (GRCm39)	utr 3 prime	probably benign
R2895:Smg1	UTSW	7	117,788,366 (GRCm39)	utr 3 prime	probably benign
R2915:Smg1	UTSW	7	117,810,102 (GRCm39)	splice site	probably benign
R3416:Smg1	UTSW	7	117,748,076 (GRCm39)	utr 3 prime	probably benign
R3417:Smg1	UTSW	7	117,748,076 (GRCm39)	utr 3 prime	probably benign
R3873:Smg1	UTSW	7	117,753,885 (GRCm39)	utr 3 prime	probably benign
R4082:Smg1	UTSW	7	117,759,469 (GRCm39)	utr 3 prime	probably benign
R4230:Smg1	UTSW	7	117,747,956 (GRCm39)	critical splice donor site	probably null
R4304:Smg1	UTSW	7	117,738,741 (GRCm39)	missense	probably benign	0.03
R4549:Smg1	UTSW	7	117,758,906 (GRCm39)	utr 3 prime	probably benign
R4571:Smg1	UTSW	7	117,738,688 (GRCm39)	missense	possibly damaging	0.72
R4638:Smg1	UTSW	7	117,795,149 (GRCm39)	utr 3 prime	probably benign
R4642:Smg1	UTSW	7	117,753,487 (GRCm39)	utr 3 prime	probably benign
R4656:Smg1	UTSW	7	117,812,174 (GRCm39)	missense	probably benign	0.00
R4754:Smg1	UTSW	7	117,755,954 (GRCm39)	utr 3 prime	probably benign
R4798:Smg1	UTSW	7	117,779,697 (GRCm39)	missense	probably benign	0.32
R4906:Smg1	UTSW	7	117,751,631 (GRCm39)	utr 3 prime	probably benign
R4978:Smg1	UTSW	7	117,753,470 (GRCm39)	utr 3 prime	probably benign
R4989:Smg1	UTSW	7	117,807,274 (GRCm39)	missense	probably benign
R4989:Smg1	UTSW	7	117,757,323 (GRCm39)	utr 3 prime	probably benign
R5026:Smg1	UTSW	7	117,792,768 (GRCm39)	utr 3 prime	probably benign
R5124:Smg1	UTSW	7	117,812,235 (GRCm39)	missense	probably benign	0.00
R5318:Smg1	UTSW	7	117,759,427 (GRCm39)	utr 3 prime	probably benign
R5356:Smg1	UTSW	7	117,794,356 (GRCm39)	utr 3 prime	probably benign
R5404:Smg1	UTSW	7	117,806,131 (GRCm39)	missense	probably damaging	1.00
R5423:Smg1	UTSW	7	117,745,294 (GRCm39)	missense	possibly damaging	0.70
R5441:Smg1	UTSW	7	117,794,304 (GRCm39)	utr 3 prime	probably benign
R5490:Smg1	UTSW	7	117,738,659 (GRCm39)	missense	possibly damaging	0.86
R5541:Smg1	UTSW	7	117,756,386 (GRCm39)	utr 3 prime	probably benign
R5564:Smg1	UTSW	7	117,789,042 (GRCm39)	utr 3 prime	probably benign
R5580:Smg1	UTSW	7	117,748,125 (GRCm39)	utr 3 prime	probably benign
R5600:Smg1	UTSW	7	117,767,107 (GRCm39)	utr 3 prime	probably benign
R5628:Smg1	UTSW	7	117,753,924 (GRCm39)	utr 3 prime	probably benign
R5646:Smg1	UTSW	7	117,811,782 (GRCm39)	missense	probably benign	0.42
R5656:Smg1	UTSW	7	117,753,887 (GRCm39)	utr 3 prime	probably benign
R5660:Smg1	UTSW	7	117,742,570 (GRCm39)	missense	probably benign	0.33
R5706:Smg1	UTSW	7	117,744,813 (GRCm39)	missense	possibly damaging	0.86
R5786:Smg1	UTSW	7	117,812,120 (GRCm39)	missense	probably benign	0.12
R5890:Smg1	UTSW	7	117,789,809 (GRCm39)	utr 3 prime	probably benign
R5912:Smg1	UTSW	7	117,753,809 (GRCm39)	utr 3 prime	probably benign
R5977:Smg1	UTSW	7	117,740,580 (GRCm39)	utr 3 prime	probably benign
R5993:Smg1	UTSW	7	117,739,732 (GRCm39)	missense	probably benign	0.33
R6161:Smg1	UTSW	7	117,762,553 (GRCm39)	utr 3 prime	probably benign
R6187:Smg1	UTSW	7	117,788,386 (GRCm39)	utr 3 prime	probably benign
R6264:Smg1	UTSW	7	117,765,310 (GRCm39)	utr 3 prime	probably benign
R6331:Smg1	UTSW	7	117,753,500 (GRCm39)	utr 3 prime	probably benign
R6561:Smg1	UTSW	7	117,765,300 (GRCm39)	utr 3 prime	probably benign
R6571:Smg1	UTSW	7	117,783,737 (GRCm39)	utr 3 prime	probably benign
R6736:Smg1	UTSW	7	117,756,389 (GRCm39)	utr 3 prime	probably benign
R6752:Smg1	UTSW	7	117,762,539 (GRCm39)	utr 3 prime	probably benign
R6777:Smg1	UTSW	7	117,788,340 (GRCm39)	utr 3 prime	probably benign
R6788:Smg1	UTSW	7	117,783,794 (GRCm39)	utr 3 prime	probably benign
R6883:Smg1	UTSW	7	117,767,403 (GRCm39)	utr 3 prime	probably benign
R6991:Smg1	UTSW	7	117,767,091 (GRCm39)	utr 3 prime	probably benign
R7056:Smg1	UTSW	7	117,745,623 (GRCm39)	splice site	probably benign
R7058:Smg1	UTSW	7	117,797,502 (GRCm39)	utr 3 prime	probably benign
R7100:Smg1	UTSW	7	117,783,743 (GRCm39)	missense	unknown
R7133:Smg1	UTSW	7	117,752,131 (GRCm39)	missense	unknown
R7221:Smg1	UTSW	7	117,782,020 (GRCm39)	missense	possibly damaging	0.86
R7229:Smg1	UTSW	7	117,776,178 (GRCm39)	missense	probably benign	0.03
R7293:Smg1	UTSW	7	117,765,322 (GRCm39)	missense	unknown
R7361:Smg1	UTSW	7	117,784,200 (GRCm39)	missense	unknown
R7438:Smg1	UTSW	7	117,795,116 (GRCm39)	missense	unknown
R7686:Smg1	UTSW	7	117,767,081 (GRCm39)	missense	unknown
R7798:Smg1	UTSW	7	117,771,162 (GRCm39)	missense	possibly damaging	0.73
R7908:Smg1	UTSW	7	117,785,357 (GRCm39)	missense	unknown
R7923:Smg1	UTSW	7	117,742,545 (GRCm39)	missense	possibly damaging	0.96
R7978:Smg1	UTSW	7	117,792,878 (GRCm39)	missense	unknown
R7997:Smg1	UTSW	7	117,772,365 (GRCm39)	missense	unknown
R7997:Smg1	UTSW	7	117,772,364 (GRCm39)	missense	unknown
R8025:Smg1	UTSW	7	117,806,212 (GRCm39)	nonsense	probably null
R8056:Smg1	UTSW	7	117,759,589 (GRCm39)	missense	unknown
R8061:Smg1	UTSW	7	117,751,610 (GRCm39)	missense	unknown
R8095:Smg1	UTSW	7	117,772,285 (GRCm39)	missense	unknown
R8198:Smg1	UTSW	7	117,744,829 (GRCm39)	missense	probably benign	0.03
R8399:Smg1	UTSW	7	117,789,794 (GRCm39)	missense	unknown
R8445:Smg1	UTSW	7	117,736,200 (GRCm39)	missense	possibly damaging	0.72
R8519:Smg1	UTSW	7	117,770,982 (GRCm39)	utr 3 prime	probably benign
R8817:Smg1	UTSW	7	117,758,887 (GRCm39)	missense	unknown
R8832:Smg1	UTSW	7	117,739,006 (GRCm39)	missense	probably benign	0.33
R8855:Smg1	UTSW	7	117,806,122 (GRCm39)	missense	unknown
R8866:Smg1	UTSW	7	117,806,122 (GRCm39)	missense	unknown
R8946:Smg1	UTSW	7	117,751,900 (GRCm39)	missense	probably null
R8954:Smg1	UTSW	7	117,806,215 (GRCm39)	missense	probably damaging	1.00
R8967:Smg1	UTSW	7	117,765,739 (GRCm39)	missense	unknown
R9072:Smg1	UTSW	7	117,783,032 (GRCm39)	missense	unknown
R9090:Smg1	UTSW	7	117,811,786 (GRCm39)	missense	unknown
R9156:Smg1	UTSW	7	117,753,884 (GRCm39)	missense	unknown
R9198:Smg1	UTSW	7	117,795,179 (GRCm39)	missense	unknown
R9240:Smg1	UTSW	7	117,739,031 (GRCm39)	missense	probably benign	0.18
R9271:Smg1	UTSW	7	117,811,786 (GRCm39)	missense	unknown
R9289:Smg1	UTSW	7	117,744,639 (GRCm39)	missense	possibly damaging	0.53
R9378:Smg1	UTSW	7	117,777,998 (GRCm39)	nonsense	probably null
R9396:Smg1	UTSW	7	117,807,303 (GRCm39)	missense	unknown
R9469:Smg1	UTSW	7	117,739,774 (GRCm39)	missense	possibly damaging	0.72
R9539:Smg1	UTSW	7	117,744,976 (GRCm39)	missense	probably benign	0.03
R9549:Smg1	UTSW	7	117,795,254 (GRCm39)	missense	unknown
R9563:Smg1	UTSW	7	117,812,208 (GRCm39)	missense	unknown
R9564:Smg1	UTSW	7	117,812,208 (GRCm39)	missense	unknown
R9597:Smg1	UTSW	7	117,812,270 (GRCm39)	missense	unknown
R9643:Smg1	UTSW	7	117,755,933 (GRCm39)	missense	unknown
R9703:Smg1	UTSW	7	117,739,744 (GRCm39)	missense	possibly damaging	0.73
R9730:Smg1	UTSW	7	117,783,004 (GRCm39)	missense	unknown
Z1088:Smg1	UTSW	7	117,777,622 (GRCm39)	missense	possibly damaging	0.96
Z1088:Smg1	UTSW	7	117,767,884 (GRCm39)	nonsense	probably null
Z1088:Smg1	UTSW	7	117,753,858 (GRCm39)	utr 3 prime	probably benign
Z1176:Smg1	UTSW	7	117,806,130 (GRCm39)	missense	unknown
Z1176:Smg1	UTSW	7	117,806,110 (GRCm39)	missense	unknown
Z1177:Smg1	UTSW	7	117,812,256 (GRCm39)	missense	unknown
Z1177:Smg1	UTSW	7	117,767,831 (GRCm39)	missense	probably null

Predicted Primers

PCR Primer
(F):5'- TTGACCAGCCGAGAAGACTAG -3'
(R):5'- TTAAGGCCCAAGATTCCTACC -3'

Sequencing Primer
(F):5'- TACAGGAATCAACTACAAAGTCTGG -3'
(R):5'- GATTCCTACCAAACTCCTCAGAATC -3'

Posted On

2014-10-01